BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,555	C→A	5.9%	V48L (GTA→TTA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,555	0	C	A	5.9%	469.1 / 3.9	183	V48L (GTA→TTA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base C (34/130); new base A (13/5); total (48/135)
Fisher's exact test for biased strand distribution p-value = 1.70e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

AGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTA  >  NZ_CP034943/2214419‑2214692

aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:3542323/140‑1 (MQ=255)

aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:3787346/140‑1 (MQ=255)

aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:565423/140‑1 (MQ=255)

aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:2847678/140‑1 (MQ=255)

aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:2428454/140‑1 (MQ=255)

aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:2529736/140‑1 (MQ=255)

aGTTTTTGGATGTATTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:3119348/140‑1 (MQ=255)

aGTTTTTGGATGATTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:2050912/140‑1 (MQ=255)

  tttttGGATGTTTTTCTCTTTAGTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTATTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2500383/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATCTTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:1754957/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:2153007/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:3702632/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:3051725/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:693936/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:1416911/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:360624/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:650718/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2506995/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:774746/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:274550/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2587228/140‑1 (MQ=255)

  tttttGGATGTTTTTCGCTTTATTACGAAACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:2016093/140‑1 (MQ=255)

  tttttGGATGTTTTTAGCTTTATTACGATACAAACAGATTTATTTAATATGCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2558616/140‑1 (MQ=255)

  ttttgGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTGAATATCCGGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:56653/140‑1 (MQ=255)

  ttgctGGCTGTTTTTCGCAGTCTCACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCCGCGAAACACCCAAATAGTCCTCCGGGCGTTTATATTGTTGAACTAATGTTGTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2929503/136‑1 (MQ=255)

  gttttGGATCTTGTTCGCTTTATTACGATACAATCCGATTGATTTAATATGATGCTGAAGCTATTACAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATTTTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2960914/139‑1 (MQ=255)

     ttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCAt                                                                                                                                   <  2:3272109/140‑1 (MQ=255)

       ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                                                 <  1:2713242/140‑1 (MQ=255)

       ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                                                 <  1:3378929/140‑1 (MQ=255)

       ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                                                 >  2:948477/1‑140 (MQ=255)

       ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGATCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAACTGCATCt                                                                                                                                 >  2:1278030/1‑140 (MQ=255)

            tttttCGCTTTATTACGATACAATCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAAATTTTTGAAATAATTTTTTTGTCACTATGTTTAAAAGTTCATCTGTACa                                                                                                                            >  2:2007273/1‑140 (MQ=255)

            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCCGCGAAACAGCCAAATCGTCTTCCGGCCGTTTATATTTTTGACATAATTTTATGGTGACTCTGTTTAGAAGTGCATCTGTTCa                                                                                                                            >  2:1717870/1‑140 (MQ=255)

            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCTGCCGTTTATATTTTTGAACTAAAGGTTTGGTCACACTGTTTACAAGTGCATCTTTACa                                                                                                                            >  2:1050354/1‑140 (MQ=255)

            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATTTTTTTTTAATAATTTTTTGGTCACTCTGTTTAAAAATTCATCTGTACa                                                                                                                            >  1:1723977/1‑140 (MQ=255)

            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                            >  2:2845211/1‑140 (MQ=255)

            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                            <  2:24725/140‑1 (MQ=255)

            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTTGTAAATCGGTATAAAAGTTCAACAGTACa                                                                                                                            >  2:2097429/1‑140 (MQ=255)

                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATATTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTTACTCTTTTTACAAGTGCATCTGTACAtttgt                                                                                                                       <  2:1409953/140‑4 (MQ=255)

                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  2:3641414/140‑1 (MQ=255)

                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  2:3569285/140‑1 (MQ=255)

                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  1:2887177/140‑1 (MQ=255)

                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  1:1575727/140‑1 (MQ=255)

                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCCTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  1:395152/140‑1 (MQ=255)

                     ttattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGa                                                                                                                   <  2:3296712/140‑1 (MQ=255)

                      tattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTAAATAATGTTTTTGTCACTCTGTTTACAATTGCATCTGTACATTGGGCAGAt                                                                                                                  >  1:1218653/1‑140 (MQ=255)

                      tattaCGATACAAAACGATTGATTTAATATCCTGCTGAAGATCTTCCAGCGAAACAGCCAAATAGTCTTCCGGACGTTTTTATTTTTGAAATAAATTTTTTTTAACTATGTTTTAAAGTTCATCATTAAAATGCGAAGaa                                                                                                                  >  1:1457332/1‑139 (MQ=255)

                            gATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCg                                                                                                            <  1:3682813/140‑1 (MQ=255)

                            gATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGGGCATCTGTACATTGCGCAGATAAAGCg                                                                                                            <  1:2581202/140‑1 (MQ=255)

                                       attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCAc                                                                                                 <  2:2773202/140‑1 (MQ=255)

                                       attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCAc                                                                                                 <  2:905005/140‑1 (MQ=255)

                                        ttgattTAATTTCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACt                                                                                                <  1:11410/140‑1 (MQ=255)

                                        ttgattTAATATCCTGCTGAGGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATGATGTTTTGGTCAGTCGGGTTACAAGTGCATCTGTACAGTGCGCCGATAAAGCGACTGCTACCACt                                                                                                >  2:2108299/1‑140 (MQ=255)

                                        ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAATTGCATCTGTAAATTTCGCAGATAAAGCGACTGCTAACACt                                                                                                >  2:798147/1‑140 (MQ=255)

                                        ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTTCATCAGTACAATGCGCAGATAAAGCGAAAGCTACCAca                                                                                                >  2:3898292/1‑139 (MQ=255)

                                        ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACt                                                                                                <  2:3003823/140‑1 (MQ=255)

                                        gtgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACCGCCAAAGAGTCTTCCGGCCGTGTTTCTGTTTGAAATGAGGGTTTGGTCACTCAGGTTACAAGTGCCTCTGTCAGTGGCGCAGATAAAGGCGCTTCTACCACt                                                                                                >  2:4023223/2‑140 (MQ=255)

                                               aaTCTCCTGCTGAAGCTCGTCCCCCGAAACACCCAAATAGTCTTCCGGCCGGTTATATTTTGGAAATAATGATTTGGGCACTCTGATTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                                                         <  2:467790/140‑1 (MQ=255)

                                                atatCCTGCTGAGGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCCGATAAAGCGACTGCTACCACTAATTCaaa                                                                                        <  1:1235743/140‑1 (MQ=255)

                                                atatCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaaa                                                                                        <  1:3960031/140‑1 (MQ=255)

                                                 tatCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaaaa                                                                                       <  1:2775228/140‑1 (MQ=255)

                                                    ccTTCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGTCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGa                                                                                    <  2:1924033/140‑1 (MQ=255)

                                                    ccTGCTGAAGCTCTTCCAGCGAAACCGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGa                                                                                    <  2:1840113/140‑1 (MQ=255)

                                                     ctgctgAAGCTCTTCCCGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   <  1:3154387/140‑1 (MQ=255)

                                                     ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   <  1:948477/140‑1 (MQ=255)

                                                     ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTGCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   <  1:1060395/140‑1 (MQ=255)

                                                     atgctgAAGCTCTTCCATCGAAACAGACAAATTGTCTTCCGGCCGTTTATTTTTTTGAAATAATGTTTTGGTCACTCTTTTTACAAGTGCATGTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   <  1:2015435/139‑1 (MQ=255)

                                                          gAAGCTCTTCCAGCGAAACAGCAAAATATTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                                              <  1:3415366/140‑1 (MQ=255)

                                                             gCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTAACACTAATTAAAAAGGATTGGAATGa                                                                           >  2:1210013/1‑140 (MQ=255)

                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:538689/140‑1 (MQ=255)

                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:652743/140‑1 (MQ=255)

                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:931102/140‑1 (MQ=255)

                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTGTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:2880019/140‑1 (MQ=255)

                                                              ctctTCCAGCGAAACAGCCAAATAGTCGTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:146058/140‑1 (MQ=255)

                                                              ctcgTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACATGTGCATGTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:3685445/140‑1 (MQ=255)

                                                                ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                                        <  1:2866439/140‑1 (MQ=255)

                                                                  tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATTTTTTGGTGACGCTGTTTAAAAGTTGAACTGGTCAATTCGCACATAAAACGACTTCTAACACAAAATCAAAAAGATTTGAATGAAcaac                                                                      >  1:1668201/1‑137 (MQ=255)

                                                                  tCCAGCGAAACACCCCAATAGTCTTCCGGCCGTTTATCTTTTTGCAATAATGGTTTGGTCACTCTGTTTACAAGTGCAGCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTCGAATGAACCAg                                                                      >  2:3791206/1‑140 (MQ=255)

                                                                      gctaaaCAGCCAAATAGACTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACTTTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                                                                  <  1:615813/137‑1 (MQ=255)

                                                                           aCAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTTTAATAAAGTTTTGGTGACTGTGTTTAAAATTGCATCTGTAAATTTCGCAGATAAAACGACTGCTAACACTAATTAAAAAGGCTTTGAATTTACCAGTTCacacaa                                                             >  1:2980716/1‑138 (MQ=255)

                                                                           aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACGCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACActc                                                             >  2:343966/1‑140 (MQ=255)

                                                                                     aGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGTTAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGa                                                   <  2:399349/140‑1 (MQ=255)

                                                                                          tCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt                                              <  1:2398127/140‑1 (MQ=255)

                                                                                          tCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt                                              <  1:1880201/140‑1 (MQ=255)

                                                                                            cGGCCGTTTATATTATTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCAGCGGGACAGTGCGCACATAACGCGACTGCTACCACTAATACAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGt                                            <  2:1013072/140‑1 (MQ=255)

                                                                                                 gTTTATATTTTTGAAATAATGTTTTTGTCACTCTGTTTAAAAGTGCATCTGTACATTTCGCAGATAAAGCGACTGCTACCACTAATTAAAAAGGATTTGAATTAAACAGTTAAAAAGCTTCGTGTTGAAACATGTAGCCt                                       >  2:2840575/1‑140 (MQ=255)

                                                                                                 gTTTATAATTTTGAAATAATGTTTTGGTCACACTGTTTACAAGTGCATCTGTACAATTCGCAGATAAAGCGACAGCTACCAATAAATAAAAAGGATTTGAATTAACCACTTAAAAAGCAGCGTGTGGAAACAtatagaca                                       >  2:1272698/1‑133 (MQ=255)

                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  1:101640/140‑1 (MQ=255)

                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGGTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  1:2791869/140‑1 (MQ=255)

                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACAGTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  2:1243926/140‑1 (MQ=255)

                                                                                                           ttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGTAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTa                             >  2:2567041/1‑140 (MQ=255)

                                                                                                           ttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTa                             >  2:3462348/1‑140 (MQ=255)

                                                                                                                        ttGGTCACTCTTTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAt                <  1:798164/140‑1 (MQ=255)

                                                                                                                             cACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGtt           <  2:718526/140‑1 (MQ=255)

                                                                                                                              aCTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGttt          >  2:1744642/1‑140 (MQ=255)

                                                                                                                              aCTCTGTTTACAAGTGAATCTGTACAGTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGttt          <  1:2074599/140‑1 (MQ=255)

                                                                                                                                tctGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTa        >  1:2432536/1‑140 (MQ=255)

                                                                                                                                    tttACACGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAGTTGTTTTAGAtt    <  1:881544/140‑1 (MQ=255)

                                                                                                                                     ttACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGAttt   >  2:3030594/1‑140 (MQ=255)

                                                                                                                                      tACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTa  >  2:3829757/1‑140 (MQ=255)

AGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTA  >  NZ_CP034943/2214419‑2214692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`