BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,207	G→T	9.0%	Q109K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,207	0	G	T	9.0%	673.0 / 11.2	297	Q109K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (114/145); new base T (36/2); total (150/147)
Fisher's exact test for biased strand distribution p-value = 6.61e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

TGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240069‑240345

tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGa                                                                                                                                            >  2:1269434/1‑140 (MQ=255)

 gttagagagagagAATGAGGATCTCTGATGTTGTTTTTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATAATTCCGAGTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                           <  2:1899288/137‑1 (MQ=255)

   aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAtt                                                                                                                                         >  1:3889010/1‑139 (MQ=255)

        agagagaAGGAGCATCTCTGATGTTGGAATTATTCGGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGATATTTGAATGGCttt                                                                                                                                    >  2:588401/1‑140 (MQ=255)

        agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                                    >  2:2866255/1‑140 (MQ=255)

        agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGAGTTTTCGGATTTTTGTTTGAAGTTTTGTGAGATTTTAATGGCttt                                                                                                                                    >  2:3535628/1‑140 (MQ=255)

         gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTGGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATggggttt                                                                                                                                   >  2:912577/1‑135 (MQ=255)

          agagaAGGAGCATCTCTGATTTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCAAATACGAATTCGATCATTCCGACTATACGTGCGTTTTAGGATTTTTGTTTTAATTTTTGGGAGATTTTAATGGCTTTTc                                                                                                                                  >  2:1867896/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGATATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCtttac                                                                                                                                  >  2:202062/1‑138 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCCTTCCGACTATACGGGCTTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTGTTc                                                                                                                                  >  2:203195/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                  >  2:2728762/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCATTTc                                                                                                                                  >  2:853402/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCCGATTTTTGTTTGAAGTTTTGGGAGCTTTGAATGGCTTTTc                                                                                                                                  >  2:2459876/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                  >  2:649740/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGGGAGAATTGAATTGCttttg                                                                                                                                  >  2:1347742/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTGCGGAATTTTGTTTGACGTTTTGTGAGATTTGAAAGGCATTTc                                                                                                                                  >  2:1317293/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTAATTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                  >  1:2214615/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTAAACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTc                                                                                                                                  >  2:3315324/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGGTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGTTTTGAATTGGTTTTc                                                                                                                                  >  1:1718034/1‑140 (MQ=255)

                ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTGGGAGATTTGAATGGCTTTTCCGtttt                                                                                                                            >  2:3836400/1‑140 (MQ=255)

                ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGtttt                                                                                                                            >  2:1549843/1‑140 (MQ=255)

                 gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGttttt                                                                                                                           <  1:697272/140‑1 (MQ=255)

                 gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGttttt                                                                                                                           <  1:1311084/140‑1 (MQ=255)

                    cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTGTAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTaaa                                                                                                                        >  1:1820425/1‑140 (MQ=255)

                    cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTaaa                                                                                                                        >  2:545379/1‑140 (MQ=255)

                       ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTACTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                     <  1:3679767/140‑1 (MQ=255)

                       ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAACa                                                                                                                     >  2:1663554/1‑140 (MQ=255)

                       ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTGCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                     <  1:3809496/140‑1 (MQ=255)

                        tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCTTTTTTAAAACaa                                                                                                                    >  2:3048020/1‑140 (MQ=255)

                         ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGTGAGATTTGAATTGCTTTTCCGTTTTTTAAACAag                                                                                                                   >  1:3580185/1‑140 (MQ=255)

                         ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAag                                                                                                                   >  2:3153371/1‑140 (MQ=255)

                            aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                                <  2:3581613/140‑1 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTGTAGTTTTTGGTGATTTTAATGGGTTTTACGTTTTTTATAAAATAGGCaatc                                                                                                           >  1:2563098/1‑138 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTTAAACAAGAGGCAAGc                                                                                                           >  1:1879424/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTAAAAAAAGAGGCaatc                                                                                                           >  2:2368979/1‑138 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTACTTTTGGGCGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                           >  1:3744262/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAACAAGAGGCACGc                                                                                                           >  2:687019/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATCGGCAAGc                                                                                                           >  2:3473500/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                           >  1:3984989/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAAATGCTTTTACGTTTTTAAAAAAAGAGGCaaac                                                                                                           >  1:2533672/1‑138 (MQ=255)

                                       attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                     <  2:3139554/140‑1 (MQ=255)

                                       attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTTAAGTTTTTGGAGATTTGTATTGGTTTTGCGTTTTTTAAAAAAGAGGCAAGCCACATa                                                                                                     >  1:1019036/1‑140 (MQ=255)

                                       attCAGAGATGCTGTTTTTGTATTTTTGTTCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                     <  2:1734655/140‑1 (MQ=255)

                                        ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCTATCATTCCGACTATAAGGGCGTTTTCGGAATTTTGTTTGAAGGTTTGGTAGATTTGAATGGCTTTTACGTTTTTAAAACACAATCCAATCCAGATaa                                                                                                    >  1:342203/1‑140 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAATTTTGTTTGAAGTTTTGGGCGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCCCATAAAcccc                                                                                               >  2:1935721/1‑139 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAAGTTTTTGGAGTTTTGTATGTCTTTTGCGGTTTTTAAAAAATAGGCAAGCCAGATAAAaccc                                                                                               >  1:352872/1‑139 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTTAAGTTTTTGGTGATTTGGATTGGTTTTCCGGTTTTAAAACAAGATGCAAGCCCCCTAAACCCGacacc                                                                                          >  1:88058/1‑138 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAATTTTTGGAGATTTTAAATGCTTTTGCGTTTTTAAAAAAAGATGCAAGCCACAAAAAACCCaca‑ac                                                                                         >  1:3236314/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGCTTTTTGTTTGTAGTTTTGGGAGATTTTTATTGCTTTTCCGTTTTTTAAACAATAGGCAAGCCAGATAAACCCCgca‑ac                                                                                         >  1:151824/1‑140 (MQ=255)

                                                     tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGaca‑acaac                                                                                      >  2:1761728/1‑140 (MQ=255)

                                                      ttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGaca‑acaaca                                                                                     <  1:1648554/140‑1 (MQ=255)

                                                      ttttGTATTTTTGTTCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCACGCCAGATAAACCCGaca‑acaaca                                                                                     <  1:1034084/140‑1 (MQ=255)

                                                       tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGAca‑acaacaa                                                                                    >  2:3917358/1‑140 (MQ=255)

                                                            aTTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAaat                                                                               <  1:553586/140‑1 (MQ=255)

                                                             ttgttGTTCTCATACTCAGTGGTTCATTCAGACTATACGGGCGTGTTGGGATTTTTGTTTGAAGTTTTGTGAGATTTGAATCGCTTTACCGTTTTTAAAACAAGTGCCAAGACAGTTAAAACCGACT‑ACATCAAAAaata                                                                              <  2:1027583/137‑1 (MQ=255)

                                                               tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGGTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCTACA‑AAAAACAAAaataat                                                                            >  2:3766114/1‑140 (MQ=255)

                                                                   ttCTAATCCGCAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  2:2154201/140‑1 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  1:1735137/140‑1 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  1:3492414/140‑1 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  1:2021449/140‑1 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  2:876229/140‑1 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  1:2579640/140‑1 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  2:141321/140‑1 (MQ=255)

                                                                   ttCTAATAAGAAGTCGATCAGTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAg                                                                        <  2:2657320/140‑1 (MQ=255)

                                                                     cTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCa                                                                      >  2:440387/1‑140 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGCCTATACGGGCGTTTTCGGATTTTTGTTTGCAGTATTGGGATAGTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGt                                                                   <  1:3178318/140‑1 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGt                                                                   <  1:1178423/140‑1 (MQ=255)

                                                                         tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAGCTGAAGGGCTTTTCCGATTTTAAAACAAGAGGCAAGCCAGATACACCCGACA‑ACAACAAAAAATAATCTAGCAAGTa                                                                  <  1:649740/140‑1 (MQ=255)

                                                                             aaGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTAAGTTTTTGGCGGTTTGTATTGCTTTTGCGTTTTTAAAACAAGAGGCAAGCCCCATAAAACCCACACACAA‑AAAAAATAATCTATCAAGTaaaat                                                              >  1:3147076/1‑138 (MQ=255)

                                                                             aaGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATTGCTTTTACCTTTTTGAAAAAAGAGGCAAGCCAGCAAAACCCGCCA‑ACAACAAAAAAAAAAAGAGCCAGTaaaat                                                              >  1:3079766/1‑138 (MQ=255)

                                                                             aaGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTTTAGTTTTTGGTGTTTTGTATTTGTTTTGCGTTTTTTAAAAAAGATGGAAACCACAAACACACGAGC‑ACAAAAAAAAAAAAAATAGCAAATaaaaa                                                              >  1:1379136/1‑138 (MQ=255)

                                                                               gTCTATCATTCCTACTATACGGGAGTTTTCGTTTTTTTGTTTTAAGTTTTGGGTGCTTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  1:2929420/140‑1 (MQ=255)

                                                                               gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  2:3846768/140‑1 (MQ=255)

                                                                               gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  2:1002684/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:1959414/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:2569563/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGCGAGAGTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:190108/140‑1 (MQ=255)

                                                                                            aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTGCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCAAGGGGTaa                                               >  2:124997/1‑140 (MQ=255)

                                                                                             cTATCCGGGCGTTGGCGGATTTTTGTTGGAAGTTGTGGGAGATTTGCATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAat                                              <  1:2119613/140‑1 (MQ=255)

                                                                                                     gCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAAACCGACA‑AAAAAAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAAATCATTCGt                                      >  2:2227857/1‑140 (MQ=255)

                                                                                                     gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGt                                      <  1:2715463/140‑1 (MQ=255)

                                                                                                      cGTTTTGGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCTTTTACGTTTTTTAAACAAGATGCAAGCCAGATAAAACCGACA‑ACAACAAAAAATAAAATAGCAAGTAAAATCCCAGCAAGGGGTAAATCATTAGtt                                     >  1:2766099/1‑140 (MQ=255)

                                                                                                      cGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGAATTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtg                                     >  2:371667/1‑139 (MQ=255)

                                                                                                       gTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACC‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTg                                    >  2:1801718/1‑140 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGGTTTTACGTTTTTAAAACAAGAGGCAACCCAGATAAAACCGACA‑AAAACAAAAAATAATATAGCAAGTAAACTCCATTCATGGTGTAATTAATTCGTTTCaa                                 >  2:1297228/1‑140 (MQ=255)

                                                                                                          gtCGGATTTTTGTTTGAAGTTTTGGGAGATTTGCATGGCTTTTCCGTTTTTAAAACAAGCGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:1133376/139‑1 (MQ=255)

                                                                                                              gATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                             <  1:731697/140‑1 (MQ=255)

                                                                                                              gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                             <  2:3595725/140‑1 (MQ=255)

                                                                                                              gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                             <  1:3701432/140‑1 (MQ=255)

                                                                                                                       tttaaGTTTTGTGATATTTGTATGGCTGTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                    <  1:2337971/137‑1 (MQ=255)

                                                                                                                       ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                    <  2:1495677/140‑1 (MQ=255)

                                                                                                                       ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                    <  2:1627501/140‑1 (MQ=255)

                                                                                                                       ttgAAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                    <  2:2051250/140‑1 (MQ=255)

                                                                                                                        tgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATaga                   <  2:1172808/140‑1 (MQ=255)

                                                                                                                          aaGTTGTGGGAGATTTGAATGGCTGTTGCGTTTTTAAAACAAGCGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  2:2273880/140‑1 (MQ=255)

                                                                                                                                    agaTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtgtg       <  1:3048020/140‑1 (MQ=255)

                                                                                                                                      aTTTTAATGGCTTTTCCGTTTTTAAAACAAGAGTCAAGCCAGATAAACCCTACA‑ACAACAAAATTTAATCTAGCAAGTAAACTCCATGCATGGGGTTATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc     <  1:924106/140‑1 (MQ=255)

                                                                                                                                      aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc     <  2:3499793/140‑1 (MQ=255)

                                                                                                                                       tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg    >  2:40772/1‑140 (MQ=255)

                                                                                                                                         tGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTa  <  1:2607211/140‑1 (MQ=255)

TGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACA‑ACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240069‑240345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`