Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,729,917 C→A 10.3% K283N (AAG→AAT lcfA ← long‑chain‑fatty‑acid‑‑CoA ligase LcfA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,729,9170CA10.3% 670.5 / 9.8 285K283N (AAG→AATlcfAlong‑chain‑fatty‑acid‑‑CoA ligase LcfA
Reads supporting (aligned to +/- strand):  ref base C (76/163);  new base A (43/2);  total (120/165)
Fisher's exact test for biased strand distribution p-value = 1.28e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

AGACAGCTTTTAATGGATGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCA  >  NZ_CP034943/2729780‑2730054
                                                                                                                                         |                                                                                                                                         
      cTTTTAATGGATGACAGATCATAATGCTGCAATTCGGGGTGATGCACAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGCTGATTTTCAGCGGTTGGAGCGGAGCCAACCTAGgaac                                                                                                                                   >  2:2922653/1‑139 (MQ=255)
                aTGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATTATTTTGAGCGTTTCGAGCGGATCAAACATAGGAACAAGAATCaat                                                                                                                         >  2:3494498/1‑138 (MQ=255)
                aTGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATGATTTTAAGCGTTTAGAGCGGATCAAAATTATGAAGAAGAATAAtt                                                                                                                         >  2:1319454/1‑140 (MQ=255)
                aTGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTTTTTTTCGAAGATTTTGATCGTTTGGAGCGGATCAAAAATAGGAAGAAAAATAAtt                                                                                                                         >  1:421035/1‑140 (MQ=255)
                aTGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTCGATGATTTTCAGCGTTTCGCGCGGATCAAAATTATgaagaagaaaaatt                                                                                                                         >  1:2504837/1‑135 (MQ=255)
                 tGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCAttt                                                                                                                        >  2:716158/1‑140 (MQ=255)
                   aCAGATCATCATGCTGCAATTCGGGGTCATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                      <  2:230880/140‑1 (MQ=255)
                   aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                      <  1:874970/140‑1 (MQ=255)
                   aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                      <  1:716158/140‑1 (MQ=255)
                   aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                      <  1:3074280/140‑1 (MQ=255)
                   aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                      <  1:283160/140‑1 (MQ=255)
                   aCAGAGCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                      <  2:2261061/140‑1 (MQ=255)
                       aTCATAATGCTGCAATTCGGGGTGATGCACAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTAAGCGTTTCGAGCGGCTCAAACATAGGAAGAAGAATCATTTAGAAGc                                                                                                                  >  2:2415873/1‑140 (MQ=255)
                        tCATAATGCTGCAATTCGGGGTGATGCACAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                 <  1:637697/140‑1 (MQ=255)
                        tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGGTTTGGTTGTCGATGATTTTCAGCGGTTCGCGCGGATGAAACGTAGGAAGAAAAATAATTTCGTAGcc                                                                                                                 >  1:2602552/1‑140 (MQ=255)
                         cATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTAAGCGTTTCGAGCGGATAAAACTTAGGAAGAAGAATCATTTCGAAGCCt                                                                                                                >  2:694484/1‑140 (MQ=255)
                          aTAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTTGCGCTGATTTTGAGCGGTTCGAGCGGCAAAAAAATTGTAAGAACAATCAATTAGAAGccga                                                                                                               >  2:324561/1‑138 (MQ=255)
                          aTAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTGGATGATTTTAAGCGTTTCGCGCGGATCAAAATTAGGAATAAGAATCATTTAGAAGccga                                                                                                               >  2:1163233/1‑138 (MQ=255)
                              tgctgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTTTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGGTTTTGGTTTATTTTCATCGGTTCGATCGGCTACAACATAGGAATAAAAATAATTTGGCAGACTAGgtt                                                                                                           >  1:2165022/1‑140 (MQ=255)
                                 tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAAt                                                                                                        >  2:2637701/1‑140 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTGGATTATTTTGAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATAATTTCGAAGCCTAGTTTAATCg                                                                                                      >  1:815808/1‑140 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATGATTTTGAGCGGTTGGAGCGGATCAAAATTATGAAGAAGAATCATTTAGAAGCCGAGTTTaaaag                                                                                                      >  1:3098926/1‑137 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTGGAGCGGGTAAAACTTATGAACAAGAATAAATTAGAAGCCAAGTTTAAtag                                                                                                      >  2:1272795/1‑138 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTAGAAGCCTAGTTTAATCg                                                                                                      >  2:3304279/1‑140 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATAATTTGGAATCCTAGTTTAATCg                                                                                                      >  2:1069721/1‑140 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGACCGGATCAAAATTAGGAATAAGAAACAATTCGAAGCCTAGTTTAAtag                                                                                                      >  2:1916175/1‑138 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTGGTTTATTTTGAGCGTTTGGAGCGGATACAACGTATGAAGAAGAATAATTTAGAAGCCTAGTTTAAtag                                                                                                      >  1:3874809/1‑138 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTGGATGATTTTAATCGTTTAGATCGGGTCAAAACTAGGAACAAAAAAAATTTCGAAGCCTTGTTTAAtag                                                                                                      >  1:778754/1‑138 (MQ=255)
                                   cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTGTGTTTTTGGCTGATTTTGAGCGTTTGGAGCGGGTCAAAATTAGGAAGAAGAAACATTTAGAAGCCTAGTTTaaaag                                                                                                      >  1:1393377/1‑137 (MQ=255)
                                     aTTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaa                                                                                                    <  1:2396065/140‑1 (MQ=255)
                                     aTTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaa                                                                                                    <  2:627314/140‑1 (MQ=255)
                                      ttCGGGGTGATGCACAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaat                                                                                                   <  2:3139347/140‑1 (MQ=255)
                                        cGGGGTGATGCAAAAGGCCAATGTCAAGTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGGTTTTCGATGTTTTTCAGCGTTTCGTGCGGATCCAAAGTAGGACGAAGAATCATTTAGTATCCGAGGTTAATCGaataa                                                                                                 >  1:1842924/1‑140 (MQ=255)
                                        cGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaataa                                                                                                 <  1:1713344/140‑1 (MQ=255)
                                          gggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                               <  1:949026/140‑1 (MQ=255)
                                          gggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTGTTTGTTTGTGGATTATTTTTAGCGTTTAGAGCCGATCAAACATAGGAAGAAGAATAATTTAGAAGCCGAGTTTAATCGaataaat                                                                                               >  1:1122369/1‑138 (MQ=255)
                                           ggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTTTCGATGATTTTCAGCGTTTAGAGCGGATCAAACTTTTGAAGAAGAATAATTTAGAAGCCTAGTTTAATCGAATAAtta                                                                                              >  2:2528181/1‑139 (MQ=255)
                                                          cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                               <  1:1758693/140‑1 (MQ=255)
                                                          cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                               <  1:1202209/140‑1 (MQ=255)
                                                          cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                               <  1:1163233/140‑1 (MQ=255)
                                                                  aTTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTGGATGATTTTAAGCGTTTCGAGCGGATAAAACATAGGAAGAAGAATAAATTATAATCCAACTTTAATAGCAAAAATAATCAATGCCGTCAGTGCgaaaac                                                                       >  1:2719336/1‑135 (MQ=255)
                                                                  aTTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTGGAGGATTTTCAGCCTTTCGAGCGGATCAAACATAGGAACAACAATAATTTAGAATCCGAGTTTAATAGAATAAATAAACACTGCCCTCACTACGtataa                                                                       >  1:3512163/1‑139 (MQ=255)
                                                                      tCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGAATATTTTCACCGTTTGGAGCGGATAAAAAGTAGGAAGAAGAAACATTTAGAAGCCTAGTTTAATCGAAAAAATCATAAATGCCGTCAGTCCGTATACGTGa                                                                   >  2:536585/1‑140 (MQ=255)
                                                                       cGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAAAGTAGGAACAAGAATCAATTAGAAGCCTAGTTTAATCGAAAAATTCATCACATCCGTCAGTCCGTATAAGTGaa                                                                  >  2:81693/1‑140 (MQ=255)
                                                                        ggTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATTATTTTAAGCGGTTAGAGCGGATCAAAATTAGGAACAAGAATAATTTAGAAGCCTAGTTTAATCGAAAAATTCAACACAGCCCTCACTGCGTATACGTGaaa                                                                 >  2:1568696/1‑140 (MQ=255)
                                                                           gCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGaaaaaa                                                              <  1:1438656/140‑1 (MQ=255)
                                                                           gCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGaaaaaa                                                              <  1:3674647/140‑1 (MQ=255)
                                                                            cACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATGATTTTCAGCGGTTCGAGCGGATCAAACATATGAAGAAGAATCATTTCGAAGCCGAGGTTAATCCAAAAATTCATAACATCCGTCAGTCCGTATACGTGaaaaaaa                                                             >  2:2034945/1‑139 (MQ=255)
                                                                                cGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTAAGCGTTTCGAGCGGATGAAACTTAGGAAGAATTATCATTTCGAAGCGTGGTTTAATCGAATAATTCATCACTGCCGGCAGTACGTATACGTGAAAAAACGGGa                                                         >  2:2837540/1‑140 (MQ=255)
                                                                                  gtacgatagTCGGTTTTTGTGTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                       <  1:2075048/133‑1 (MQ=255)
                                                                                  gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                       <  2:1630292/140‑1 (MQ=255)
                                                                                  gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                       <  2:3811353/140‑1 (MQ=255)
                                                                                  gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                       <  2:3572256/140‑1 (MQ=255)
                                                                                       agagTCGGTTTTTGTTTTTCGATGATTTTAAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATAATTTGGAAGCCTATTTTAATCGAAAAAATAATCAATGCCCTCAGTCCGTATAAGTGAAAAAACGGTACGATTcc                                                  >  2:3375809/1‑140 (MQ=255)
                                                                                       agagTCGGTTTTTGTTTGTAGATTATTTTCAGCGTTTCGAGCGGATAAAACTTAGGAAGAACAATCATTTCGAAGCCTAGTTTAAACGAATAATTCATCACTGCCGTCAGTCCCTATACGTGAAAAAACGGGAACATGcc                                                  >  2:3854819/1‑140 (MQ=255)
                                                                                        gagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGccc                                                 <  1:3494498/140‑1 (MQ=255)
                                                                                        gagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGccc                                                 <  1:1026968/140‑1 (MQ=255)
                                                                                         agTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAATAAAAATTTCGTATCCAATTTTAATCGAATAATTAATCACTTCCGGCAGTCCCTATACGTTAAAAAAAGGGACGATGCCCa                                                >  2:2701910/1‑140 (MQ=255)
                                                                                           tCGGTTTTTGTTTTTCGATGATTTTGAGCGTTTCGAGCGGATCAAACTTTGGCAGAAAAAAAATTTCGAAGCCTAGGTTAATCGCATAAATAAACACATCCGTCAGTCCCTATAACTGTAAAAAAAGGACGAttaccacc                                              >  2:2280859/1‑133 (MQ=255)
                                                                                                     tttgtCGATGATTTTCAGCGTTTCGAGCGGATCAATCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAg                                    <  2:918494/140‑1 (MQ=255)
                                                                                                         tctatgatTTTCAGCGTTTCGAGCGGATCAAAATTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  1:3344926/137‑1 (MQ=255)
                                                                                                         tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  2:1179099/140‑1 (MQ=255)
                                                                                                         tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  1:244214/140‑1 (MQ=255)
                                                                                                         tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  1:666157/140‑1 (MQ=255)
                                                                                                         tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  1:2657067/140‑1 (MQ=255)
                                                                                                         tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                >  1:1519989/1‑140 (MQ=255)
                                                                                                         tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  1:2236697/140‑1 (MQ=255)
                                                                                                         tCGAGGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTACGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                <  1:2848544/140‑1 (MQ=255)
                                                                                                              gatTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttcct                           <  2:40824/140‑1 (MQ=255)
                                                                                                                 tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGACGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                        <  1:2922653/140‑1 (MQ=255)
                                                                                                                 tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGACTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                        <  1:2133241/140‑1 (MQ=255)
                                                                                                                 tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                        <  1:3955503/140‑1 (MQ=255)
                                                                                                                 tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                        <  2:152271/140‑1 (MQ=255)
                                                                                                                   tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                      <  2:432653/140‑1 (MQ=255)
                                                                                                                      gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                   <  2:446846/140‑1 (MQ=255)
                                                                                                                      gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                   <  1:2415873/140‑1 (MQ=255)
                                                                                                                      gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                   <  2:1810026/140‑1 (MQ=255)
                                                                                                                      gCGTCTCGAGCCGCTCAAACATAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATACATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCACCACTTTTTCAGCCCCTTCCTTCCCCTCg                   <  1:1907041/140‑1 (MQ=255)
                                                                                                                          ttCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACa               >  1:2528218/1‑140 (MQ=255)
                                                                                                                             gaccGGATCAAACTTACGAAGAAGAATCATTTCGAAGCCTAGTTTAATCTAATAATTCATCACTGCCCACAGTCCGTATACGTGAAAAAACGGGACGAGGCCCAGCACCTTTTCAGCCCCTTCCTTCACATCCTACATcc            <  2:2212098/137‑1 (MQ=255)
                                                                                                                                cGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAg         <  1:2701910/140‑1 (MQ=255)
                                                                                                                                cGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAg         <  1:542750/140‑1 (MQ=255)
                                                                                                                                cGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAg         <  1:231075/140‑1 (MQ=255)
                                                                                                                                   aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg      >  2:196752/1‑140 (MQ=255)
                                                                                                                                   aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg      >  1:336981/1‑140 (MQ=255)
                                                                                                                                     cAAACTTAGGAATAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACTATTCCCAGCACTTTTTCAGCACCTTCCTTCACATCGTACATCCAAGCGGAg    <  2:273934/140‑1 (MQ=255)
                                                                                                                                     cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg    <  1:3293394/140‑1 (MQ=255)
                                                                                                                                     cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg    <  1:211540/140‑1 (MQ=255)
                                                                                                                                     cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg    <  1:3414506/140‑1 (MQ=255)
                                                                                                                                     cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg    <  1:1374203/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGATTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAATACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  2:1713791/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAATCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  1:3787519/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  1:1002010/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  1:1182177/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  2:857182/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  2:773877/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  1:1568696/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  1:3702686/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  1:440272/140‑1 (MQ=255)
                                                                                                                                       aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca  <  2:1458442/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AGACAGCTTTTAATGGATGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCA  >  NZ_CP034943/2729780‑2730054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: