BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,219,792	C→G	11.8%	P377R (CCG→CGG)	oxdC →	oxalate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,219,792	0	C	G	11.8%	441.3 / 8.7	169	P377R (CCG→CGG)	oxdC	oxalate decarboxylase
Reads supporting (aligned to +/- strand): ref base C (89/57); new base G (2/21); total (91/78)
Fisher's exact test for biased strand distribution p-value = 1.84e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

AATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGT  >  NZ_CP034943/3219661‑3219929

aaTCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTaa                                                                                                                                   <  1:3613109/140‑1 (MQ=255)

aaTCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTaa                                                                                                                                   <  1:501791/140‑1 (MQ=255)

  tCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTaaaa                                                                                                                                 <  1:293917/140‑1 (MQ=255)

    tttAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGGTTGCAATGCTTCCTGAATCATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTaaaaaa                                                                                                                               >  2:191741/1‑140 (MQ=255)

             gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAg                                                                                                                      >  1:2676776/1‑140 (MQ=255)

              aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                                                                     >  2:1903495/1‑140 (MQ=255)

              aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                                                                     >  2:2860861/1‑140 (MQ=255)

              aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                                                                     >  2:3963593/1‑140 (MQ=255)

              aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                                                                     <  2:1650478/140‑1 (MQ=255)

              aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTGGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                                                                     >  2:3984614/1‑140 (MQ=255)

              aTCATTATGCCGATGTATATTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGAATTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                                                                                     <  2:1688117/140‑1 (MQ=255)

                         gATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAAc                                                                                                          >  2:1521337/1‑140 (MQ=255)

                         gATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAAc                                                                                                          >  2:277522/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                                                                         >  2:3772356/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                                                                         >  2:2448144/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                                                                         >  1:3606333/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                                                                         >  2:3847648/1‑140 (MQ=255)

                           tttATCTTTAAACAAATGGTTTGCATTGCTTCCTCAAACATTATTTAAACAGCATTTTTATTTAGTAAAAGAATTTACTTATATTCTTTCCAAATAAAAGCATCCGGTTGTAAAAAATAAATGCATTAAATAAAAAACGa                                                                                                        <  2:1341627/138‑1 (MQ=255)

                               tCTTTAAACCAATGGCTTGCAATGCTTCCTGAATCAGTAGTTCAACAGAATCTTTATTTAGGAAAAGACTTTACTGATATTCTTTAGAAAGAAAAGCAGCGGGTTGTAAAAAAGAAATGCAGTACATAAAAAACGACCAg                                                                                                    >  2:1943384/1‑140 (MQ=255)

                               tCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTGGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAATAAGAAATGCAGTAACTAAAAAACGACCAg                                                                                                    >  2:2276552/1‑140 (MQ=255)

                                atttAAACCAATGGTTTGCATTGCTTCGTTAAAAATTAGTTCAACAGCATCTTTATTTAGCAAACGACTTTACTGATATTCTTACGCAACAAAAGAATCCGTTTGTAAAAAAGAAATGCATTAAATAAAAAACGACCAGc                                                                                                   <  2:202153/139‑1 (MQ=255)

                                    aaaCCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTga                                                                                               >  2:1570710/1‑139 (MQ=255)

                                        catgggCTGGCAATGCTTCCTGAAACATTAGTTCAACCGCATCTTGTTTGAGGAAAAGACTTTACTGATATTCTTTCCAAAGAAAAGCATCCGTTTGTAAAAAACAAATCCATTAAATAAAAAACGACCAGCTTGCagag                                                                                           <  2:1868858/136‑1 (MQ=255)

                                        cactGGCTTGAACTGATACCTGACACATTTGTTCAACAGAATCTTGATTTCGGAAACCACTTTACTGTTCTTATTTCGAAATAAAAGCATCCTTTGGTAAAAAAGAAATGCAGTAAATAAAACACGACCAGCTTGCagag                                                                                           <  2:2126916/137‑1 (MQ=255)

                                        cAATGGCTTGCATTTATTCCTTAACAATTTGTTCAACTGAATCTTGATTTAGCAAAAGACTTTACTGATATTCTTACGAAACAAAAGCTTCCGTTTGTAAAAAAAAAAGGAAGTAAATAAAACACGACCAGCTTGCagag                                                                                           <  2:770010/140‑1 (MQ=255)

                                         aaTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagaga                                                                                          >  2:323370/1‑140 (MQ=255)

                                         aaTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagaga                                                                                          >  2:950720/1‑140 (MQ=255)

                                         aaTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAAAGAACAGCTTGCagaga                                                                                          >  2:1331947/1‑140 (MQ=255)

                                         aaTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCGTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagaga                                                                                          >  2:3940907/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:981463/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:3550822/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  1:1441942/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  1:1259696/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:444334/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGAAGTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:3183158/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCGTCCTGAAACATTAGTTCAACAGCATCGTGTGTTAGGACAAGACTTTACTGATATTCTTGCGAAAGAACAGCATCGGGGTGTAAAAAAGAAATGCAGTAACTAAAAAACTACCAGCGTGCagagag                                                                                         >  2:2556212/1‑140 (MQ=255)

                                          aTGGCTTGAAAGTCTGACTGAAACATTATTTAAACAGAATCTTTATTTAGTAAAAGACTTTCCTTATTTTCTTTCGAAAAAAAAGCATCGTGTTTTAAAAAAGAAATGCACTAAATAAAACACGACCAGCTTGCagagag                                                                                         <  2:2676776/140‑1 (MQ=255)

                                           tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagctgtc                                                                                        >  1:2701467/1‑135 (MQ=255)

                                           tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagctgtc                                                                                        >  1:3977219/1‑135 (MQ=255)

                                           tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGc                                                                                        >  1:2372578/1‑140 (MQ=255)

                                           tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGc                                                                                        >  1:1458066/1‑140 (MQ=255)

                                           tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGc                                                                                        >  1:94331/1‑140 (MQ=255)

                                             gCTTGAAATGCTTCCTCAAACATTATTTAAACAGCATATTTATTTAGCAAAAGAATTTACTGATATTCTTTCGAAAGAAAAGCAACCGGTTTTAAAAAAGAAAGGCAGTAAAAAAAACACGACCAGCTTGCAGAGAGCaa                                                                                      <  2:1441942/140‑1 (MQ=255)

                                                tgaaaTGCTTCCTGAAACTTTATTTCAACCGAATCTTGATTTTGTAAAAGACTTTCCTGATTTTCTTTCGAAACAAAAGCATCGTGTTGTAAAAAAGAAATGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCg                                                                                   <  2:1385388/137‑1 (MQ=255)

                                                tccactGCTTCCACAAACATTAGTTCAACCGCAACTTGATTTAGGAAACGACTTAACGCATATTCTTTCCCAATAAAACCATCCGGTTGTAAAAAACAAATCCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCg                                                                                   <  2:105479/135‑1 (MQ=255)

                                                tGCCATGCTTCCTGAAACATTTGTTCAAGCGCACCTTGACTTGGGCAAAGACTTTACTGATGTGCTTTCAAAAGAAAAGCACCCAGTAGTGAAAAAGAAATGCAGTAAATAAAAGACTTGCGGCTTGCAGAGAGCACTCg                                                                                   >  1:1017329/1‑140 (MQ=255)

                                                tGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCg                                                                                   >  1:951426/1‑140 (MQ=255)

                                                tGCAATGCTTCATTAAAAATTTGTTCAAAAGCATATTTATTTCGTAAAATAATTTACTCATATTCTTCCCAAAGAAAAGCATCCGTTTGAAAAAAACAAATGAATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCg                                                                                   <  2:1384485/140‑1 (MQ=255)

                                                  cAATGCTTCCTCAAACATTATTTCAACATCATCTTGATTTAGTAAACGACTTTACTGATTTACTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATCCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:1977957/140‑1 (MQ=255)

                                                  aattGCTACCTCAAACATTACTACAAACCAATATTGATTTCGCAAAATACTTAACTTATATTCTTTCCAAATAAAACCAACCTTTTTTAAAAAATAAATGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  1:2784786/137‑1 (MQ=255)

                                                     tGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTtctc                                                                              >  2:1266780/1‑140 (MQ=255)

                                                     tGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTtctc                                                                              >  2:433697/1‑140 (MQ=255)

                                                     tGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTtctc                                                                              >  2:3299904/1‑140 (MQ=255)

                                                       cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                            >  2:3973490/1‑140 (MQ=255)

                                                       cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                            >  2:3338565/1‑140 (MQ=255)

                                                         tCCTGAACCAGTTGTTCACCAGCAACTGCATTTAGCAAACGACTTTACTGATTCTCATTCCAAAGAAAAGCACCCGTTTGTAAAAAAGAACTGCCCTAAATAAAACACGACCAGCTTGCAGAGAGCACTCGTTCTCTGCa                                                                          <  2:1351348/140‑1 (MQ=255)

                                                           ctcaaaCTTTAGTCCAACATCATTTTTTTTTAGTAAAAGATTTTAGTTATATTCTTACTAAAGAAAAGCATCGGTTTTAAAAAAATAAATGCATTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg                                                                        <  1:2989629/137‑1 (MQ=255)

                                                             caaaCATTTGTCCAACCGCATCTTGATTTATCAAAACACTTTACTGATATTCTTGCGAAACAAACGCATCCGTTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGcc                                                                      <  2:3686944/139‑1 (MQ=255)

                                                                   ttattacAACAGAATGTTTATTTAGGAAAAGACTTTACTTATATTCTTTCTAAAGAAAAGCATCCGGTTGTAAAAAATAAATGCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                <  2:743064/134‑1 (MQ=255)

                                                                   ttAGTTAAACTGAATTTTTATTTTTGAAAATATTTTACTGATATTCTTTCCAAACAAAAGCATCGGGTTGTAAAAAACAAATGCATAAAAAAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                <  1:2521762/140‑1 (MQ=255)

                                                                     aGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAg                                                              >  1:1412429/1‑140 (MQ=255)

                                                                      gTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAgg                                                             >  2:2945329/1‑140 (MQ=255)

                                                                      gTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTTCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAgg                                                             >  2:3028386/1‑140 (MQ=255)

                                                                            cAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGt                                                       >  1:176199/1‑140 (MQ=255)

                                                                            cAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGt                                                       >  1:1642183/1‑140 (MQ=255)

                                                                                aTCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCa                                                   >  1:3591679/1‑140 (MQ=255)

                                                                                 tCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAg                                                  >  2:3164736/1‑140 (MQ=255)

                                                                                   tttaTTTATAAAAACATTTTACTTATATTCTTTCTAAACAAAAGCACCGGTTTTTAAAAAACAAAGCCAGAAAATAAACAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTg                                                <  1:1570710/137‑1 (MQ=255)

                                                                                    tGATTTATAAAAACAATTACCTGATTTGCTTTCTAAACAAAAGCATCCGTTTTTAAAAAACAAATCCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGa                                               <  1:218075/140‑1 (MQ=255)

                                                                                                aGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAAttt                                   >  2:2568842/1‑140 (MQ=255)

                                                                                                aGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAAttt                                   >  2:2976301/1‑140 (MQ=255)

                                                                                                      tACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCaa                             >  1:1903934/1‑140 (MQ=255)

                                                                                                      tACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCaa                             >  1:1236501/1‑140 (MQ=255)

                                                                                                       aCTGATATTCTTTCCAAATAAAAGCATCGGGTTGTAAAAAATAAATGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCaaa                            <  1:3958246/140‑1 (MQ=255)

                                                                                                           atatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGc                        >  2:3187100/1‑140 (MQ=255)

                                                                                                           atatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGc                        >  1:1113306/1‑140 (MQ=255)

                                                                                                           atatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGc                        >  1:821710/1‑140 (MQ=255)

                                                                                                           atatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGc                        >  2:1762216/1‑140 (MQ=255)

                                                                                                           atatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGc                        >  1:3211946/1‑140 (MQ=255)

                                                                                                                 tttCGAAATAAAAGAATCCGTTTTTAAAAAATAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTg                  <  2:1669952/140‑1 (MQ=255)

                                                                                                                 tttCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTg                  <  2:884822/140‑1 (MQ=255)

                                                                                                                 tttCGAAAGAAAAGCATCCGGTGTTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTg                  <  2:3420560/140‑1 (MQ=255)

                                                                                                                 tttCCAAACAAAACCATCCGGTTTAAAAAAAGAAATGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTg                  <  1:180688/140‑1 (MQ=255)

                                                                                                                   tctaaagaaaAGCATCGGGTTGTAAAAAAGAAATGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCg                <  2:404263/137‑1 (MQ=255)

                                                                                                                    ccaaacaaacGAATCGGTTTTTAAAAAATAAATGCATTAAATAAAACACGACCAGCTGGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCgg               <  1:1016314/138‑1 (MQ=255)

                                                                                                                     gaaagaaaAGCATCCGGTTTTAAAAAATAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGc              <  2:2032060/140‑1 (MQ=255)

                                                                                                                      aaagaaaAGCATCCGGTTTAAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  2:3016027/140‑1 (MQ=255)

                                                                                                                      aaacaacAGCATCCGTTTTAAAAAAACAAATGAATAAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  1:3687283/140‑1 (MQ=255)

                                                                                                                      aaacaaaAGCATCCGGTTTAAAAAAAAAATTCCATAAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  1:3950124/140‑1 (MQ=255)

                                                                                                                      aaacaaaAGAATCCTTTTTTAAAAAATAAATTCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  1:1256410/140‑1 (MQ=255)

                                                                                                                      aaaaaaaaGCATCCGTTTCTAAAAAAAAAATCCACAAAAAAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  1:1662139/140‑1 (MQ=255)

                                                                                                                             cccacccGGTTGAAAAAAAGAAATGCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:2727490/135‑1 (MQ=255)

                                                                                                                             agcagcCGGTTGTAAAAAACAAATGCATAAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:3393478/140‑1 (MQ=255)

                                                                                                                             aGCATCCGTTTGTAAAAAACAAATCCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:3318401/140‑1 (MQ=255)

                                                                                                                             aGCATCCGGTTGTAAAAAATAAATGCATTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:3783274/140‑1 (MQ=255)

                                                                                                                             aGCATCCGGTTGTAAAAAATAAATGCAGTAATTAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAAAGCTTATTGCGGCTCTTtata      <  1:3009343/140‑1 (MQ=255)

                                                                                                                             aGCATCCGGTTGTAAAAAAGAAATGCTTAAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  2:2253695/140‑1 (MQ=255)

                                                                                                                             aGCATCCGGTTGAAAAAAACAAATCCAGAAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:3818466/140‑1 (MQ=255)

                                                                                                                             aGCATCCGGGTGAAAAAAACAAAGGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCATTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:2880441/140‑1 (MQ=255)

                                                                                                                                 tCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACTACCAGCTTTCAGAGAGCAATCTTTATCTGCAAGCCTTTATAAGGAGATGTACAAGTGAAACATCAAAATTCTTCAAAACGCTTAATGCGGCTCTTTATAAAGt  >  1:1934258/1‑140 (MQ=255)

AATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGT  >  NZ_CP034943/3219661‑3219929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`