Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,719,864 T→G 5.6% S79R (AGC→CGC)  EO946_RS19145 ← YwdI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,719,8640TG 5.6% 619.2 / 2.4 271S79R (AGC→CGC) EO946_RS19145YwdI family protein
Reads supporting (aligned to +/- strand):  ref base T (112/133);  new base G (2/24);  total (114/157)
Fisher's exact test for biased strand distribution p-value = 9.77e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCGC  >  NZ_CP034943/3719725‑3720002
                                                                                                                                           |                                                                                                                                          
aaCGCTGTCCATTGCAGCGCTCCAAGAACCAATTTCACATAGCTCACCCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTGTCTTTCTCCTGCTTTCGATATTTGTGTAATCCCGCGCt                                                                                                                                            >  1:2595485/1‑140 (MQ=255)
aaCGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCt                                                                                                                                            >  1:3018798/1‑140 (MQ=255)
   gCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATACAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTcc                                                                                                                                         <  2:1021476/140‑1 (MQ=255)
     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                       >  2:2031934/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                     >  1:3179239/1‑140 (MQ=255)
       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                     >  2:3497632/1‑140 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTccc                                                                                                                                        >  2:1926786/1‑132 (MQ=255)
            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                                >  2:1710520/1‑140 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAACGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                           >  1:2398590/1‑140 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                           >  1:3331576/1‑140 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                           >  1:3091814/1‑140 (MQ=255)
                 cgcACCAAGAACCAATTTCACATAGCTCAACATTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                           <  2:728847/140‑1 (MQ=255)
                 cgcACCAACAACCAATTTCACATAGATCAACATTTCTCATTAAAAATCAAATAAAGACTCTCAGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCAGCGCTTCACATCATTTTTTCca                                                                                                                           <  2:345793/140‑1 (MQ=255)
                   cACCCAGAACCAATTTCACATCGCTCAACCTTTCTCATTAAAAATCAACTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATGTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                         <  1:3195307/140‑1 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATCTGTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                         >  1:135862/1‑140 (MQ=255)
                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                         <  1:3472916/140‑1 (MQ=255)
                      cAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCatcatc                                                                                                                      >  1:2303397/1‑140 (MQ=255)
                            ccAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTgg                                                                                                                <  1:2700280/140‑1 (MQ=255)
                             cAATTTCACATATCTCAACCTTTCTCATTAAAAATCAAAGAAAGACTCTCAGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTGAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                               <  2:844807/140‑1 (MQ=255)
                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                               <  2:2229075/140‑1 (MQ=255)
                             cAAGTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                               <  1:3037377/140‑1 (MQ=255)
                              aaTTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTc                                                                                                              >  1:3976957/1‑140 (MQ=255)
                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                            >  2:1396846/1‑140 (MQ=255)
                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                            >  1:2523576/1‑140 (MQ=255)
                                    acaTAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTGTTTCTCCTGGTTTCGATATTTGTTTAATCCCGCGCTTCCCCTCATTTTTTCCATAAGAAGCTGGTCTGTGGa                                                                                                        >  1:512303/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCTTGCTCTTCGTGCTTCTCTTTCTCCTTGGTTCGATATTTGTGTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGATGTTCTGTCGATTGCgg                                                                                                  >  1:1012926/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGCTATTTGTTTAATCCCGCGCTTCCCCTCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                  >  1:2449622/1‑140 (MQ=255)
                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                  >  1:3764552/1‑140 (MQ=255)
                                               ccTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCgg                                                                                             <  1:2031934/140‑1 (MQ=255)
                                               ccTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCgg                                                                                             <  1:1657192/140‑1 (MQ=255)
                                                  ttCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAg                                                                                          >  1:59398/1‑140 (MQ=255)
                                                  ttCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAg                                                                                          >  1:458984/1‑140 (MQ=255)
                                                  ttCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAg                                                                                          >  1:2944190/1‑140 (MQ=255)
                                                  ttCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAg                                                                                          >  1:1680023/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                        >  2:422725/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                        >  2:3097023/1‑140 (MQ=255)
                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                        >  1:188350/1‑140 (MQ=255)
                                                         tAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCgg                                                                                   >  1:1341278/1‑140 (MQ=255)
                                                             aaTCAAATAAAGACTCTCCGTGTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                               <  2:2128428/140‑1 (MQ=255)
                                                                  aagaaaGACTCGCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                          <  2:3389299/137‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                          <  2:389155/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                          <  2:3432943/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                          <  1:3596955/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                          <  2:1140210/140‑1 (MQ=255)
                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                          <  2:2007539/140‑1 (MQ=255)
                                                                     aaaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCgg                                                                       <  2:2551622/140‑1 (MQ=255)
                                                                      acgACTCTCCGTTTCCGTCCTCGTCCTGCTTCTCGTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  2:3286020/138‑1 (MQ=255)
                                                                      aaGCCTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  1:539957/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  1:3795868/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  1:2637094/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  1:1508942/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  2:1853784/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  1:578480/140‑1 (MQ=255)
                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  1:699097/140‑1 (MQ=255)
                                                                      aaGACTCTCCGGTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                      <  2:1741558/140‑1 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                               >  1:3730209/1‑92 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                               >  2:593652/1‑140 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                               >  2:3216891/1‑140 (MQ=255)
                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                               >  2:1745053/1‑140 (MQ=255)
                                                                                 tttGCGTCCTCTTCCTGTTTCTGTTTCTCCTGCTTTGGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCgggg                                                           <  1:3910917/140‑1 (MQ=255)
                                                                                 tttCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCgggg                                                           <  2:1291910/140‑1 (MQ=255)
                                                                                   tCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGgcg                                                         <  2:1365979/140‑1 (MQ=255)
                                                                                   tCCGTCCTCTTCCTGCTTCTCGTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCCTCATTTTTTCCCTCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGgcg                                                         <  2:3147546/140‑1 (MQ=255)
                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                     >  2:1613355/1‑140 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                   <  1:2813733/140‑1 (MQ=255)
                                                                                         ctctTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                   <  2:3404687/140‑1 (MQ=255)
                                                                                         ctcgTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGGTGCTCGGCTGTATGCGGGGCGCGCTcg                                                   <  2:3068680/140‑1 (MQ=255)
                                                                                         ctcgTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                   <  2:569013/140‑1 (MQ=255)
                                                                                         ctcgTCCTCCTAGTCTTTCTCCTGCGTCGGATAGTTTTGGACGCCCGCGCGTCCCATCGTGTTTTCCATCCTGATCTGGTCTGCCGATGGCGGTGCGTCAGGACTCGGCGTGCTGCGCGGCTGTATGCGGGGGGCGCTcg                                                   <  2:939267/140‑1 (MQ=255)
                                                                                               cTGCTTCTCTTTCTCCTGCGTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGtct                                             <  2:2810919/140‑1 (MQ=255)
                                                                                               cTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGtct                                             <  1:2062311/140‑1 (MQ=255)
                                                                                                  cTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCg                                          >  2:166218/1‑140 (MQ=255)
                                                                                                    tctctctcTCCGGCCTTCGATATGTGTTTAATCCCGCGCTTCCCATCATTATTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                        <  2:2419278/140‑1 (MQ=255)
                                                                                                    tctcTTTCTCCTGCATTCGATATTTGTATAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGAGCGGCAGTACTCGGCGTGCTGCTCGGCTGTATGCCTGGCGCCCTCTCGGTCTCCGGc                                        >  2:3789013/1‑140 (MQ=255)
                                                                                                    tctcGTGCTCCTGCCGTCGATCTTTGTTTAAGCCCGCGCGTCCCATCCTTTGTTCCCTCATCAGCTGGTCTGTCGATGGCGGGGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                        <  1:2236368/140‑1 (MQ=255)
                                                                                                      tctTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTg                                      >  2:544810/1‑140 (MQ=255)
                                                                                                      tctTTCTCCTGCTTTCGATATTGGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTCTGCGGGGCGCGCTCGCGGTCTCCGGCTg                                      >  2:497655/1‑140 (MQ=255)
                                                                                                         ttCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATc                                   <  1:1883702/140‑1 (MQ=255)
                                                                                                          tctcCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATcc                                  >  2:1820204/1‑140 (MQ=255)
                                                                                                            tcCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCGATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGGGCTCGCGGTCTCCGGCTGATACAg                                <  2:4008646/140‑1 (MQ=255)
                                                                                                            tcCTGCTGTCGATCGGTCGTTCATCCCGCGCTGCCCCTCATTTGTTGCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAg                                <  2:2302690/140‑1 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                              <  1:887806/140‑1 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                              <  1:3394377/140‑1 (MQ=255)
                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                              <  1:1279635/140‑1 (MQ=255)
                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                            >  1:1781545/1‑140 (MQ=255)
                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                            <  1:406058/140‑1 (MQ=255)
                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                            >  1:3362579/1‑140 (MQ=255)
                                                                                                                 cTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacga                           >  1:1102866/1‑140 (MQ=255)
                                                                                                                 cTTTCGATATGTGTTTAATCCCGCGCTTCCCATCCTTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCCGGACTCGGCGTGCTGCTCGGCTGTCTGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacga                           >  2:3059202/1‑140 (MQ=255)
                                                                                                                  tttCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacgac                          <  1:288786/140‑1 (MQ=255)
                                                                                                                    tCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGt                        <  1:876941/140‑1 (MQ=255)
                                                                                                                    tCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGt                        >  1:1809607/1‑140 (MQ=255)
                                                                                                                     cGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTc                       <  1:3582275/140‑1 (MQ=255)
                                                                                                                      gagatTTCTTTAATCCCCCCCGTCCCCTCACTTTGTCCCCCCTCAGCTGCTCTGTCGATGGCGGTGCGGCAGGCCTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTca                      <  2:3833456/137‑1 (MQ=255)
                                                                                                                      gATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTca                      <  2:2694490/140‑1 (MQ=255)
                                                                                                                          tttGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacaca                  <  2:2451334/140‑1 (MQ=255)
                                                                                                                                tAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAAc            >  2:3035913/1‑140 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc        <  2:3682100/140‑1 (MQ=255)
                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc        <  1:328062/140‑1 (MQ=255)
                                                                                                                                         gcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTATATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCg   <  1:3816546/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCGC  >  NZ_CP034943/3719725‑3720002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: