BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,906,067	A→C	12.4%	I42S (ATT→AGT)	EO946_RS20100 ←	DUF3255 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,906,067	0	A	C	12.4%	664.1 / 13.1	359	I42S (ATT→AGT)	EO946_RS20100	DUF3255 family protein
Reads supporting (aligned to +/- strand): ref base A (181/110); new base C (56/2); total (246/113)
Fisher's exact test for biased strand distribution p-value = 1.79e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

AGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCTTT  >  NZ_CP034943/3905928‑3906206

aGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTaa                                                                                                                                             <  2:1501722/140‑1 (MQ=255)

  tACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAAtt                                                                                                                                           >  1:2653158/1‑140 (MQ=255)

   aCTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTc                                                                                                                                          >  2:935381/1‑140 (MQ=255)

    cTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTcc                                                                                                                                         >  1:2728755/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTc                                                                                                                                      >  1:432309/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:2933810/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:1741594/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:138260/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:3965959/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:2469494/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGCTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:1027339/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCGCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:3948449/1‑140 (MQ=255)

        gggAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGAGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCa                                                                                                                                     >  2:3058816/1‑140 (MQ=255)

          gAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCAGGGGTAATTCCCTCATc                                                                                                                                   >  2:290181/1‑140 (MQ=255)

            aaCCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATccc                                                                                                                                 >  1:2127942/1‑140 (MQ=255)

            aaCCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTACAATCACATCCGTATCCACAACCTCGTATTTGGTAAATGTGACTCTTGCTGAGATCAATCCGTCCATGGGTCCCGGGGTAATTCACTCATccc                                                                                                                                 >  2:1443088/1‑140 (MQ=255)

                    tGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCg                                                                                                                         <  1:1789706/140‑1 (MQ=255)

                    gctATGCCATCAAAGGCAGTTGCACCTCTCCACGTCCTACAATCACTTCCGTCTCCACCACCTCGTCTTGGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCg                                                                                                                         <  1:3340530/138‑1 (MQ=255)

                     gTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGa                                                                                                                        <  1:2553761/140‑1 (MQ=255)

                     gTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGa                                                                                                                        <  2:4017286/140‑1 (MQ=255)

                       aTGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGaaa                                                                                                                      <  1:1954557/140‑1 (MQ=255)

                        tGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAAt                                                                                                                     >  2:130661/1‑140 (MQ=255)

                        tGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAAt                                                                                                                     >  2:1343394/1‑140 (MQ=255)

                        tGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAAt                                                                                                                     >  2:38790/1‑140 (MQ=255)

                         gCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATa                                                                                                                    >  1:1040763/1‑140 (MQ=255)

                          ccATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATaa                                                                                                                   <  2:1692891/140‑1 (MQ=255)

                           cATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATaaa                                                                                                                  >  2:2231054/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTc                                                                                                        >  1:3297169/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATGTc                                                                                                        >  1:2064159/1‑140 (MQ=255)

                                       ttGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCct                                                                                                      >  1:1196530/1‑140 (MQ=255)

                                       ttGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCct                                                                                                      >  1:2182812/1‑140 (MQ=255)

                                         gCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCctct                                                                                                    >  2:1515907/1‑140 (MQ=255)

                                         gCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCctct                                                                                                    >  2:270894/1‑140 (MQ=255)

                                           aCCTCCCCTCTTCCTTCAACCACTTCCGTCTCCACCACCACGTCTTGGGTAAAGCTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGGTCCGAAATAAACAGGCATTTCCTCttt                                                                                                  <  2:1849011/140‑1 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  1:3791704/1‑140 (MQ=255)

                                                  ctctTCCTTCAATCTCTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCAGTCCCTCATCCCACGTTCCGAAATAATAAGGCATTTCCTCTTTttggtgt                                                                                           >  1:3959071/1‑140 (MQ=255)

                                                     ttccttcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGa                                                                                        <  2:1002792/140‑1 (MQ=255)

                                                       ccttcAATCACTTCCGTCTCCACCACCTCGTCTTTTGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACa                                                                                      <  2:3295991/140‑1 (MQ=255)

                                                       ccttcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACa                                                                                      >  1:1878569/1‑140 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAgac                                                                                   >  1:1587737/1‑138 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:82146/140‑1 (MQ=255)

                                                                 cTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAc                                                                            >  2:1105459/1‑140 (MQ=255)

                                                                 cTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAc                                                                            <  2:2566791/140‑1 (MQ=255)

                                                                 cTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAAGTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAc                                                                            <  2:468919/140‑1 (MQ=255)

                                                                  ttCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACg                                                                           >  1:3889659/1‑140 (MQ=255)

                                                                  ttCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGGAATAAAAAGGCATGTCCTGGGGTAGTTGGTGTCAGCCCGTTAACg                                                                           >  2:2397450/1‑140 (MQ=255)

                                                                  ttCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACg                                                                           >  1:3594568/1‑140 (MQ=255)

                                                                   tCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGt                                                                          <  2:663815/140‑1 (MQ=255)

                                                                    ccGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  1:2347289/140‑1 (MQ=255)

                                                                    ccGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  2:1735592/140‑1 (MQ=255)

                                                                    ccGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  1:2895186/140‑1 (MQ=255)

                                                                      gTCTCCACCACCTCGTCTTTGTTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGAt                                                                       <  2:2008157/140‑1 (MQ=255)

                                                                         tcCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGca                                                                    <  2:1040763/140‑1 (MQ=255)

                                                                         tcCACCACCTCGTCGTTGGTACAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGca                                                                    <  1:3393846/140‑1 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGa                                                                                        >  2:770057/1‑117 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  2:1890831/1‑140 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGGTGCTGATTTCACGCCGTCCATGGGTCCCGCGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  2:2447433/1‑140 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGTGACTGTTGGTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAAGTGATGCACAcag                                                               <  1:312200/140‑1 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACAcag                                                               >  1:505814/1‑140 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACAcag                                                               <  1:259844/140‑1 (MQ=255)

                                                                                   cGTGTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAGTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGtt                                                          >  1:1344662/1‑140 (MQ=255)

                                                                                   cGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGtt                                                          >  1:547465/1‑140 (MQ=255)

                                                                                     tCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTaa                                                        >  1:28698/1‑140 (MQ=255)

                                                                                     tCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTaa                                                        >  1:3538598/1‑140 (MQ=255)

                                                                                        ttGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAg                                                     >  1:1002948/1‑140 (MQ=255)

                                                                                         aggTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGc                                                    >  1:2456746/2‑140 (MQ=255)

                                                                                              aaGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAAt                                               >  2:2848765/1‑140 (MQ=255)

                                                                                               aGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATg                                              >  2:3738674/1‑140 (MQ=255)

                                                                                                ggTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGc                                             <  2:505814/140‑1 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcaca                                         >  1:1699932/1‑140 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcaca                                         >  2:706097/1‑140 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcaca                                         >  2:533987/1‑140 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcaca                                         >  2:425254/1‑140 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcaca                                         >  2:3673727/1‑140 (MQ=255)

                                                                                                       gTTGCTGATTTCACGACGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAttt                                      <  1:1506554/140‑1 (MQ=255)

                                                                                                          gCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGtt                                   >  2:577292/1‑140 (MQ=255)

                                                                                                          gCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGtt                                   >  1:1399463/1‑140 (MQ=255)

                                                                                                           cTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGttt                                  >  2:2759138/1‑140 (MQ=255)

                                                                                                              aTTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGAAAAATGCCACATTTGTTtctc                               >  2:3526580/1‑140 (MQ=255)

                                                                                                                  cACGCCGTCCATGGGTCCCGGGGTACTTCCCGCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTGTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATGTGTTTCTCAAgg                           >  2:3618489/1‑140 (MQ=255)

                                                                                                                   aCGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGt                          <  2:2292659/140‑1 (MQ=255)

                                                                                                                      ccGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGAt                       <  2:1190736/140‑1 (MQ=255)

                                                                                                                       cGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATc                      <  1:757989/140‑1 (MQ=255)

                                                                                                                        gTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCa                     <  1:229257/140‑1 (MQ=255)

                                                                                                                        gTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCa                     >  2:3200625/1‑140 (MQ=255)

                                                                                                                         tCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCAc                    >  1:2986610/1‑140 (MQ=255)

                                                                                                                                 tCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCttt            >  1:1226368/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:3542880/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:886304/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:1253419/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATATCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:3725480/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:3348187/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:1440101/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:77733/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:9232/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATACCCTCATCCCACGTTCCGAAATAAAAAGGCATGGCCTCTTTTTGTTGTTGACACCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATCCCCCATTTGTTTCTCAAGGTGATCACCATCCtttgc          >  2:3123227/1‑138 (MQ=255)

                                                                                                                                      ggttAATTACCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcctcc       <  2:3257001/137‑1 (MQ=255)

                                                                                                                                      gggTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcctcc       <  2:2145268/140‑1 (MQ=255)

                                                                                                                                          aaTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCtt   >  1:323576/1‑140 (MQ=255)

                                                                                                                                           aTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCttt  >  1:1150903/1‑140 (MQ=255)

AGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCTTT  >  NZ_CP034943/3905928‑3906206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`