BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,413	A→C	15.7%	T86P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,413	0	A	C	15.7%	303.1 / 14.1	167	T86P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (50/84); new base C (4/28); total (55/112)
Fisher's exact test for biased strand distribution p-value = 6.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

AGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607274‑607552

aGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCaa                                                                                                                                             <  2:592416/140‑1 (MQ=255)

 gCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                                                                            <  1:3224639/140‑1 (MQ=255)

 cccACGGGAGCAACCGTGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCAGCCGGACCAACCGCAGCGACGGGAGCCACGGCAGCTACCGGGGAAGCCGGCCCAGCCGGACCAAc                                                                                                                                            <  1:774921/139‑1 (MQ=18)

  ccACGGGAGCTACCGGGGAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           <  2:2991663/140‑1 (MQ=255)

        gAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcg                                                                                                                                     <  1:3627149/140‑1 (MQ=255)

         aGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcga                                                                                                                                    <  1:3917903/140‑1 (MQ=255)

             aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                                                                                >  1:1113150/1‑140 (MQ=255)

             aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                                                                                <  1:1901157/140‑1 (MQ=255)

             aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                                                                                <  1:95433/140‑1 (MQ=255)

             aCCGGGGAAGCCGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                                                                                <  1:2028797/140‑1 (MQ=255)

             aCCGGAGAACCAGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGCGAACCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGTCGGACCAGCCGGACCAACCggagcgacagg                                                                                                                                <  2:405977/140‑1 (MQ=17)

                   gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  1:1304911/140‑1 (MQ=255)

                     aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                                                                        >  1:1714099/1‑140 (MQ=255)

                     aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                                                                        >  1:871388/1‑140 (MQ=255)

                      gacggACCAGCGGTAGCGCGGGGAGAGAATGGAGCTACCGGGGAAGCCGGACCAGCCGGACAAACCGTCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGAACAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       <  1:1469337/137‑1 (MQ=17)

                      gCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       <  1:1741124/140‑1 (MQ=255)

                        tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgaccggagcgacaggag                                                                                                                     >  1:387894/1‑140 (MQ=255)

                         ggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                                                                                    >  1:710359/1‑140 (MQ=255)

                               gCCGGAGCGCCGGGCGCGACCGGAGCCACCGGGGAAGCCGGACCAGCCGGACCACCCGGACCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                              <  2:2658895/140‑1 (MQ=17)

                               gCCGGAGCGACGGGAGCGCCAGGAGCTACCGGGGACGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                              <  2:3855135/140‑1 (MQ=255)

                               gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCACCCGGAGCGACCGGAGCGACAGGAGCTACCgg                                                                                                              >  2:3035094/1‑140 (MQ=255)

                               gCCGGAGCGACGGGAGCCACAGGAGCTCCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                              <  2:1277320/140‑1 (MQ=255)

                               gCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCAGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                              <  2:2295750/140‑1 (MQ=255)

                               gCCGGAGCGACGGGAGCCACAGGACCTACCGGGGAACCCGGACCAGCCGGACCACCCGGAGCGTCGGGAGCCCCGGGAGCTAGCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCgg                                                                                                              <  2:3423967/140‑1 (MQ=14)

                                ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  1:3459890/140‑1 (MQ=255)

                                ccGGAGCCCCGGGAGCCACAGGAGCCACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  1:2047623/140‑1 (MQ=14)

                                    aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACAGGAGCGACCGGAGCCACGGGAGCTACCGGGGCAGCCGGACCACCCGGAGCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAg                                                                                                         >  1:2939548/1‑140 (MQ=14)

                                         caggAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTACAGGGGAACCCGGa                                                                                                    >  2:2669656/3‑140 (MQ=14)

                                         cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGACGCCGGa                                                                                                    >  2:1949356/1‑140 (MQ=255)

                                         cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGCGCTACCGGGGAAGCCGGa                                                                                                    >  1:1903447/1‑140 (MQ=255)

                                         cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACGGGAGCTACCGGGGAAGCCGGAGACGCGGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    >  2:120800/1‑140 (MQ=14)

                                          gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:742625/140‑1 (MQ=255)

                                          gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:3248237/140‑1 (MQ=255)

                                              gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCAACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCCACCGGAGCAACCGGGGCGGCCGGagcagc                                                                                               >  2:2795030/1‑140 (MQ=11)

                                                  cAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgga                                                                                           >  1:2627847/1‑140 (MQ=255)

                                                   aGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                                                                          <  1:3700934/140‑1 (MQ=255)

                                                        cTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCCCCGGAGCGACAGGAGAGAGAGGAGCGACAGGCCAAGCCGGACCTGCCggagcgagc                                                                                     >  2:1070901/1‑138 (MQ=14)

                                                        cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAGGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGGGCTGCCGCGGAAGCCGGACCGGCCggggcgacg                                                                                     >  2:3867920/1‑139 (MQ=17)

                                                          aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacgggagccacagggg                                                                                                                     <  2:2076673/106‑3 (MQ=255)

                                                          aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCggagcgacagg                                                                                   >  1:1823230/1‑140 (MQ=255)

                                                           ccGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGAGGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagga                                                                                  <  1:921221/140‑1 (MQ=255)

                                                           ccGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagga                                                                                  <  1:1089205/140‑1 (MQ=255)

                                                               ggcagccggccccgccggaccACCCGGACCCCCCGGAGCCACGGGAGCTACCGGCGAAGCCGGACCAGCCGGACGACCCGGAGGGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                                                                              <  2:662210/137‑1 (MQ=17)

                                                               ggcagccggaccacccggaccACCCGGAGCGACGGGAGCCACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                                                                              <  2:774118/137‑1 (MQ=14)

                                                                gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACCGGAGATACCGGGGACGCCGGACCAGCCGGACCGACCGGCGCGACCGGGGCGACCGCAGCTACCGGGCACCCCGGGCCGGCCggtgcgaccggcgcgag                                                                             >  2:936746/1‑139 (MQ=14)

                                                                 tagcgggaccagccggaccCACCGGCGCGCCGGGAGCCACGGGAGGTTCCGGGGCAGGCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                            <  1:2871381/139‑1 (MQ=17)

                                                                   gccggcccagccggaccaACCGGAGCGACGGGCGCCACGGGAGCGACCGGGGAACCCGGACCACCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          <  2:1608627/140‑1 (MQ=17)

                                                                    ccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagga                                                                         <  2:237505/140‑1 (MQ=255)

                                                                     cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          >  2:2601259/1‑138 (MQ=255)

                                                                          ccgccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgaca                                                                   <  2:710359/138‑1 (MQ=255)

                                                                              cggaccaCCCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGCAGCCGGACCAGCCGGACCCCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                                                               <  2:2947762/140‑1 (MQ=17)

                                                                                gaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt                                                             <  2:871388/140‑1 (MQ=255)

                                                                                gaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt                                                             <  2:1714099/140‑1 (MQ=255)

                                                                                 accaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                            >  1:2582135/1‑140 (MQ=255)

                                                                                    aaCCGGAGCGACGGGAGCCACCGGTGCTACCGGGGAAGCCGGACCAGCCTGACCGACCGCACCGACCGGCGCGACAGGAGCTACCGGGGAAGCCGGACGAGCCGGAGCGACCGGAGCGCCCGGCGCGACTGGAGGTGCCg                                                         >  1:684381/1‑140 (MQ=14)

                                                                                     cccGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCGACCgg                                                        <  1:2114401/139‑1 (MQ=255)

                                                                                     cccGGACCCACCGGAGCCACTGGACCTAACGGTGAAGCCGGACCCGCCGGACCAACCGGAGAGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        <  2:170154/139‑1 (MQ=14)

                                                                                     aCCGGAGCGCCGGGAGCCACGGGAGCTCCCGGGTAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        <  1:2394383/140‑1 (MQ=255)

                                                                                      ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACCGGAGCTACCggg                                                       >  1:2372539/1‑140 (MQ=255)

                                                                                      ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:1704578/140‑1 (MQ=255)

                                                                                      ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:2701952/140‑1 (MQ=255)

                                                                                          cgCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:220758/139‑1 (MQ=255)

                                                                                          aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:3118056/140‑1 (MQ=255)

                                                                                          aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:3564683/140‑1 (MQ=255)

                                                                                          aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:1927486/140‑1 (MQ=255)

                                                                                          aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:3180190/140‑1 (MQ=255)

                                                                                                gggAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAc                                             <  2:1804211/140‑1 (MQ=255)

                                                                                                    gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGGGCGACAGGAGCTACCGGGGAACCCGGACCCCCCGGAGCGACAGGAGCGACCGGTGCGGCAGGTCCGACCGGGGACGCTGGACCacc                                         >  1:2798476/1‑138 (MQ=17)

                                                                                                       aCGGGAGCTACCGGGGAAGCCGGACCAGCGGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGTAGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTgg                                      <  1:2981066/140‑1 (MQ=255)

                                                                                                        cGGGTGCTACCGGGGAACCCGGACCACCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGa                                     <  1:620424/140‑1 (MQ=17)

                                                                                                         gggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGGCCAGCTGGAg                                    >  1:3190780/1‑140 (MQ=255)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGGGCTACCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTAGCGGGCCATCAGGAACAGCGGGAGCGACg                               >  2:1926621/1‑140 (MQ=14)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                               >  1:3693106/1‑140 (MQ=255)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACCGGAGCGCCCGGCCAAGCGGCAGCAGCGGTAGccgcc                               >  1:1396580/1‑136 (MQ=14)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                               >  1:3796001/1‑140 (MQ=255)

                                                                                                                cccGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACTGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:447639/139‑1 (MQ=255)

                                                                                                                acgggggATGCCGGACAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:1792665/137‑1 (MQ=255)

                                                                                                                acaggcgAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:195149/134‑1 (MQ=255)

                                                                                                                aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             >  2:1485765/1‑140 (MQ=255)

                                                                                                                 ccGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGTAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGa                            >  2:2064359/1‑140 (MQ=255)

                                                                                                                 ccGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGCGAACGGAGCTACCGGGCACGCGCGCCCCGCGGGAGCGACgggg                            >  2:796116/1‑139 (MQ=14)

                                                                                                                 ccGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGa                            <  1:304863/140‑1 (MQ=255)

                                                                                                                      gacgccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:3175080/137‑1 (MQ=255)

                                                                                                                      gaacacggaccagccggaccaACCGGAGAGAAAGGAGCCAAAGGAGCTACCGGGTAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGTGACGGGAGCGAc                       <  1:1361518/135‑1 (MQ=14)

                                                                                                                      gAAGCCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGCAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  2:3358737/140‑1 (MQ=255)

                                                                                                                      gAAGCCGGACCCGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:2687100/140‑1 (MQ=255)

                                                                                                                      gAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACTGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:3712773/140‑1 (MQ=255)

                                                                                                                      gAACCCGCACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  2:2384942/140‑1 (MQ=255)

                                                                                                                      ccacccggaccagccggaccaCCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCGACTGGAGCGACTGGAGCTCCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:1344228/136‑1 (MQ=14)

                                                                                                                         ggcgcacccgccggaacaCCGGGAGCCACTGGTGCCACAGGTGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAgg                    <  1:256349/135‑1 (MQ=14)

                                                                                                                         gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCGGGGCCAGCTGGGCCAGAGGCGGCGACGGGCGAGACggg                    >  2:1507624/1‑140 (MQ=17)

                                                                                                                          ccggcccagccggaccaACCGGAGCGACCGGCGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGa                   <  2:3515291/140‑1 (MQ=17)

                                                                                                                          ccggacccgccggaccaCCCGGAGCGACAGGAGCCACCGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGa                   <  2:1884588/140‑1 (MQ=14)

                                                                                                                          caggacaagccggaccaACCGGAGCGCCAGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGa                   <  2:1884480/138‑1 (MQ=14)

                                                                                                                           cggaccagccggaccaCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                  <  1:3581391/140‑1 (MQ=255)

                                                                                                                           cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                  >  1:1504095/1‑140 (MQ=255)

                                                                                                                              accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa               >  2:3441117/1‑140 (MQ=255)

                                                                                                                                       accaACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGCAAGCCGGACCACCCGGAGCGACAGGAGAGCCAGGATCTAAAGTAGCTAGCTGGGAAGCGGGACCAGAAGGAACGACCGGAGCGACAGGAGCTACCggggcac      >  1:2273010/1‑137 (MQ=17)

                                                                                                                                           aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGTCAGGAGCTACCGGGGAAGCGGGAGCAGCTGGAGCGACGGGAGCTACAGCAGCTACCGGGGAAGcagt  >  2:1011970/1‑137 (MQ=18)

                                                                                                                                           aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCgg  >  2:2467888/1‑140 (MQ=255)

AGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607274‑607552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`