Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 27,592 A→C 7.9% T391P (ACG→CCG)  dnaX → DNA polymerase III subunit gamma/tau

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494327,5920AC 7.9% 680.7 / 8.9 271T391P (ACG→CCG) dnaXDNA polymerase III subunit gamma/tau
Reads supporting (aligned to +/- strand):  ref base A (113/125);  new base C (2/31);  total (115/156)
Fisher's exact test for biased strand distribution p-value = 2.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CCATCCCCGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGACCTGCT  >  NZ_CP034943/27453‑27731
                                                                                                                                           |                                                                                                                                           
ccATCCCCGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAaacaa                                                                                                                                             <  2:4117559/140‑1 (MQ=255)
  aTCCCCGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACg                                                                                                                                           <  2:3478931/140‑1 (MQ=255)
  aTCCCCGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACg                                                                                                                                           <  1:1634180/140‑1 (MQ=255)
  aTCCCCGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACg                                                                                                                                           <  2:3706208/140‑1 (MQ=255)
    ccccGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCCAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCGCAAAACAACggg                                                                                                                                         >  1:550870/1‑140 (MQ=255)
    ccccGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACggg                                                                                                                                         >  1:3608726/1‑140 (MQ=255)
    ccccGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACggg                                                                                                                                         >  2:144137/1‑140 (MQ=255)
    ccccGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACggg                                                                                                                                         >  2:4222116/1‑140 (MQ=255)
    ccccGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAAAggg                                                                                                                                         >  2:4452239/1‑140 (MQ=255)
    ccccGTATCTTTTTTGAAGTGGCAGGGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGAGTTGCCGGATGTCGATATGCTGATGAAAAATATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACggg                                                                                                                                         >  1:1901273/1‑140 (MQ=255)
         tATCTTTTTTTAAGTGGCAGAGGTGAAGATATGCCAAACGTATCATCAAACAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACCGGCATaa                                                                                                                                    >  1:4204495/1‑140 (MQ=255)
         tATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGACGTGGAGCGGCTCAAAACAACGGGCATaa                                                                                                                                    >  2:3252190/1‑140 (MQ=255)
         tATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATaa                                                                                                                                    >  2:378233/1‑140 (MQ=255)
         tATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATaa                                                                                                                                    >  2:584444/1‑140 (MQ=255)
           tCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATaaaa                                                                                                                                  >  1:4296642/1‑140 (MQ=255)
           tCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATaaaa                                                                                                                                  >  1:2441485/1‑140 (MQ=255)
              tttttGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAagca                                                                                                                               <  2:3119634/140‑1 (MQ=255)
              tttttGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAagca                                                                                                                               <  2:5578999/140‑1 (MQ=255)
                tttGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACg                                                                                                                                           >  1:5418944/1‑126 (MQ=255)
                tttGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAagcagc                                                                                                                             >  1:5489117/1‑140 (MQ=255)
                         gCAGTGTTGAAGATTTGCCAATCGTCTCATCAATCATCATCTGACTTGCAGGAAGTCGATATGCTGATGAAAAAAATTCAGAAGCTATAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGAAGCTGCGGAAAg                                                                                                                    >  2:2391004/1‑140 (MQ=255)
                         gCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAg                                                                                                                    <  2:2077315/140‑1 (MQ=255)
                         gCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAg                                                                                                                    >  1:5291242/1‑140 (MQ=255)
                                     aTTTTCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGTAAGTCGATATGCTTATTAAAAAAATTCAGCAGCTCGAGCAGGATGTGGAGCGGCTAAAAACAACGGGCATAAAAGCCGCGGCGGAAAGCCCGAAAAAAGa                                                                                                        <  1:2954430/140‑1 (MQ=255)
                                       ttGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGaaa                                                                                                      >  2:209994/1‑140 (MQ=255)
                                       ttGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGaaa                                                                                                      >  2:5117470/1‑140 (MQ=255)
                                         gCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAAcg                                                                                                    <  2:1766378/140‑1 (MQ=255)
                                         gCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAAcg                                                                                                    <  2:1352497/140‑1 (MQ=255)
                                             aaattctaATCAATCAGCAGCTTACTAGCCCGAAGACCATATCCTGATTAAAAAAATTAACCAGCTCTAGCAGGAAGTGGACCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCTAAAAAAGAAAcgccgc                                                                                                <  2:3368158/136‑1 (MQ=255)
                                             aaCGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAAcgccgc                                                                                                <  2:2640270/140‑1 (MQ=255)
                                             aaCGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAAcgccgc                                                                                                <  2:3844262/140‑1 (MQ=255)
                                               cttctcATAAATCAGCAGATGACTTGCCGGAAGTCGATATGATGATTAAAAAAATACAGCAGCTCGAGCAGGAAGTTGAGCGGCTAAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCgt                                                                                              <  1:1145160/138‑1 (MQ=255)
                                               cGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATTAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCgt                                                                                              <  2:4666231/140‑1 (MQ=255)
                                               cGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATGCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAACCAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCgt                                                                                              <  2:333056/140‑1 (MQ=255)
                                                     atcaatcaGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCa                                                                                        <  2:4640239/140‑1 (MQ=255)
                                                      tcaatcaGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGCGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCaa                                                                                       <  2:1546909/140‑1 (MQ=255)
                                                      tcaatcaGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCaa                                                                                       <  2:5734243/140‑1 (MQ=255)
                                                      acaatcaGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCaa                                                                                       <  2:5336075/139‑1 (MQ=255)
                                                        catcaGCCGCTGCCGCGCCGGAAGTCGATATGCTTATTAAAAAAAGTCAGCCGCGCTCGCAGGAAGTGGAGCGGCTCAAAACCACGGCCATAAAAGCAGCGGCGGAAACCCCGAAAAAAGACACGCCGCGTGTGCCAAAg                                                                                     <  1:5061788/139‑1 (MQ=255)
                                                          tcaGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAggg                                                                                   >  1:4860764/1‑140 (MQ=255)
                                                              cagcTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTggg                                                                               <  2:1948018/140‑1 (MQ=255)
                                                               cgcTGACTTGCCGGAAGTCTATATGCTGATTAAAAAAATTCAGCCGCTCGAGCAGGACGTGGAGCGGCTCAAAACACCGGGCATAAAAGCAGGGGCGGAAAGCCCGAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGa                                                                              <  1:1683565/139‑1 (MQ=255)
                                                               agcTGACTTGCCGTAAGTCGATATGCTGATGAAAAAAATTCAGCCGCTCGAGCAGGAAGTGGCGCGGCTCAAAACACCGGCCATAAACGCCGCGGCGGAACGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGa                                                                              <  1:5051016/140‑1 (MQ=255)
                                                               agcTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATGCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGCCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGa                                                                              <  1:5692901/140‑1 (MQ=255)
                                                                 cTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAg                                                                            <  2:4326314/140‑1 (MQ=255)
                                                                 cTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAg                                                                            >  1:1431985/1‑140 (MQ=255)
                                                                        gCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAAtt                                                                     >  1:1947453/1‑140 (MQ=255)
                                                                              aGTCGATATGCTTATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACACCGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAg                                                               <  2:5417746/140‑1 (MQ=255)
                                                                              aGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAATGGTGGGAAGTCCAATTACAAAg                                                               <  2:2857315/140‑1 (MQ=255)
                                                                              aGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAg                                                               <  2:5642787/140‑1 (MQ=255)
                                                                              aGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAg                                                               >  2:2499077/1‑140 (MQ=255)
                                                                              aGTCGAGAGGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAACACAACGGGCATAAAAGCAGCCGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAg                                                               <  2:3936936/140‑1 (MQ=255)
                                                                                tcgttttGCTGATGAAATAAAGTCAGCAGCTCGAGCCGGACGTGGAGCGGCTCAAAACAACGGGCATAAACGCAGCGGCTGATAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAgcg                                                             <  2:1186114/134‑1 (MQ=255)
                                                                                tCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAgcg                                                             <  2:3412642/140‑1 (MQ=255)
                                                                                        cTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACg                                                                                                                                           <  2:1662196/54‑1 (MQ=255)
                                                                                          gatgaAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCa                                                   >  2:2260906/1‑140 (MQ=255)
                                                                                           atgaAAAAAATTCAGCAGCTCGAGCTGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCAGCGTGTGCGAAAGGGTGGGAAGTCCAATTACCATGCGCCCGTAGGCAg                                                  >  1:1543838/1‑140 (MQ=255)
                                                                                            tgaAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGa                                                 >  2:658981/1‑140 (MQ=255)
                                                                                             gaAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGaa                                                >  1:2846515/1‑140 (MQ=255)
                                                                                                     ttCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGaa                                        >  1:4923254/1‑140 (MQ=255)
                                                                                                     ttCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGaa                                        >  1:1979280/1‑140 (MQ=255)
                                                                                                     ttCAGCAGATCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGaa                                        <  2:899407/140‑1 (MQ=255)
                                                                                                           atcTCGAGCATGATGTTGAGCGGATAAAAAAAAGGTGCATAAAAGCCGGGGGGCAAAGCCCGAAAAAAGACACGCCGCGTGTGCCAAAGGGGGGGACGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTg                                  <  1:1769077/138‑1 (MQ=255)
                                                                                                             cTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaa                                >  1:2107263/1‑140 (MQ=255)
                                                                                                             cTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaa                                >  1:4729295/1‑140 (MQ=255)
                                                                                                              tCGAGCCGGAAGTGGCGCGGCTCAAAACACCGGGCATAAAAGCAGCGGCGGAAAGCCCTAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaag                               <  1:2286511/140‑1 (MQ=255)
                                                                                                              tCGAGCAGGAAGTGGCGCGGCTCAAAACACCGGGAATAAAAGCAGCGGCGGAAAGCCCTAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaag                               <  1:2703173/140‑1 (MQ=255)
                                                                                                              tCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaag                               <  2:4071767/140‑1 (MQ=255)
                                                                                                              tCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaag                               <  1:1314866/140‑1 (MQ=255)
                                                                                                              gcGAGGAGGCCGTGGCGCGGCTCAAAACAAGGCCCATAAACCCAGGGGGGGAATCCCCGAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCAAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTgaag                               <  1:3522515/139‑1 (MQ=255)
                                                                                                              acGAGCAGGCAGTGCCGCTGCACAACACAACGGGCATAAACGCCGCGGCGGAAAGCCCTAAAAACCACACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGCCAGAATTCATGAAATTTTgaag                               <  1:4073081/139‑1 (MQ=255)
                                                                                                                     ggcaGTGGCGCGGCTCAAAACACCGGGCATAAACGCAGCGGCGGAACGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTa                        <  1:5633730/137‑1 (MQ=255)
                                                                                                                     ggACGTGGAGCGGCTCACACCACCGGGCATAACAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTa                        <  1:2070407/140‑1 (MQ=255)
                                                                                                                     ggAAGTGGAGCGGCTCAAAACAACGGGCATAAACCCCGCGGCGGAACGCCCGAAAAAAGACACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTa                        <  1:4421859/140‑1 (MQ=255)
                                                                                                                     ggAAGTGCAGCGGCTAAAAACACCGGCCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTa                        <  1:584444/140‑1 (MQ=255)
                                                                                                                        aGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACga                     >  1:2386590/1‑140 (MQ=255)
                                                                                                                            gAGCGGCTCACAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATGTTGAAGGAAGCTACGAGAcc                 >  1:749885/1‑140 (MQ=255)
                                                                                                                            gAGCGGCTCAAAACACCCGGCATAAACGCAGCGGCGTAAAGCCCGAAAAAACACACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  1:2557506/140‑1 (MQ=255)
                                                                                                                            gAGCGGCTCAAAACAACGGGCATAAACGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  2:1973748/140‑1 (MQ=255)
                                                                                                                            gAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 >  1:1004874/1‑140 (MQ=255)
                                                                                                                            gAGCGGCTCAAAACAACGGCCATAAAACCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  1:5157201/140‑1 (MQ=255)
                                                                                                                            gAGCGGCTAAAAACACCGCCCATAACACCAGCGGCGGAAACCCCCAAAAAAGAAACCCCGCGTGTGCCAAAGGGTGGGAAGTCAAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  1:3115166/140‑1 (MQ=255)
                                                                                                                            gAGCGGCTAAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  2:5508894/140‑1 (MQ=255)
                                                                                                                            gAGCGGATCAAAACAACGGGCATAAACGCAGCGGCGTAAAGCCCGAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  1:951052/140‑1 (MQ=255)
                                                                                                                            gAGCGCCTCAAACCAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAcc                 <  2:71823/140‑1 (MQ=255)
                                                                                                                                ggCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGAc                  >  1:4144604/1‑135 (MQ=255)
                                                                                                                                 gCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATc            >  2:5291661/1‑140 (MQ=255)
                                                                                                                                 gCTCAAAACAACGGCCATAAACCCAGCGGCGGAAAGCCCCAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATc            <  1:846430/140‑1 (MQ=255)
                                                                                                                                  cTCAAAACAACGGGCATTAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGGGTGCCAACGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGACTTCCTGAAATTTTGACGGAAGCTACGAGACCGGATCt           >  2:4808513/1‑140 (MQ=255)
                                                                                                                                  cTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCt           >  2:2597258/1‑140 (MQ=255)
                                                                                                                                      aaaCACCGGGCATAAAAGCAGCGGCGGAAAGCCAGAAAAAACACACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAc       <  1:3240261/140‑1 (MQ=255)
                                                                                                                                      aaaCAACGGGCATAACACCAGCGGCGGAAAGCCCGAAAAAACAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAc       <  2:2425301/140‑1 (MQ=255)
                                                                                                                                      aaaCAACGGGCATAAAAGCAGCGGCGTAAAGCCCGAAAAAACACACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAc       <  1:5563832/140‑1 (MQ=255)
                                                                                                                                      aaaCAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAACGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAc       <  2:2738757/140‑1 (MQ=255)
                                                                                                                                      aaaCAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAc       <  2:4961255/140‑1 (MQ=255)
                                                                                                                                      aaaCAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAc       <  2:3717349/140‑1 (MQ=255)
                                                                                                                                       aacaacGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGAcc      >  2:2158927/1‑140 (MQ=255)
                                                                                                                                           acGGGCATAAACGCAGCGGCGCAAAGCCCGAAAAAAGACACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGACCTGCt  <  1:4469601/140‑1 (MQ=255)
                                                                                                                                           acGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGACCTGCt  <  2:54324/140‑1 (MQ=255)
                                                                                                                                           acGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCACGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGACCTGCt  <  1:3993623/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CCATCCCCGTATCTTTTTTGAAGTGGCAGTGGTGAAGATTTGCCAAACGTCTCATCAATCAGCAGCTGACTTGCCGGAAGTCGATATGCTGATGAAAAAAATTCAGCAGCTCGAGCAGGAAGTGGAGCGGCTCAAAACAACGGGCATAAAAGCAGCGGCGGAAAGCCCGAAAAAAGAAACGCCGCGTGTGCCAAAGGGTGGGAAGTCCAATTACAAAGCGCCCGTAGGCAGAATTCATGAAATTTTGAAGGAAGCTACGAGACCGGATCTTGACCTGCT  >  NZ_CP034943/27453‑27731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: