BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,211	G→T	13.1%	A107A (GCC→GCA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,211	0	G	T	13.1%	945.3 / 47.9	464	A107A (GCC→GCA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (151/237); new base T (74/2); total (225/239)
Fisher's exact test for biased strand distribution p-value = 3.68e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 467 total aligned reads displayed.

AGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGA  >  NZ_CP034943/240073‑240347

  agagagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATTGCt                                                                                                                                       >  1:2596302/1‑140 (MQ=255)

    agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGTAGTTTTGGGAGATTTTAATGGGttt                                                                                                                                     >  1:3194555/1‑140 (MQ=255)

    agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCttt                                                                                                                                     >  2:470866/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGGTTTTc                                                                                                                                   >  1:4366189/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGTGATTTGAATGGGtttta                                                                                                                                   >  1:4134452/1‑139 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAATTTTTTGAGATTTTAATTTCtttta                                                                                                                                   >  1:1583904/1‑139 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTTAATGTCtttta                                                                                                                                   >  1:5779301/1‑139 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCtttta                                                                                                                                   >  2:2307973/1‑139 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                                   >  1:1231597/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                   >  2:5393214/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGTTTTTTTTTTGTAGTTTTTTGAGATTTGAATTGCttttg                                                                                                                                   >  1:3118606/1‑139 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAATGTTTTGGAGATTTTAATGGCGTTTc                                                                                                                                   >  1:2433431/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGTAGTTTTGGGAGGTTTGTATTGCTTTTc                                                                                                                                   >  1:3732565/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTTAAGTTTTGGGAGATGTGAATGGCTTTTc                                                                                                                                   >  1:4499999/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATGGCtttta                                                                                                                                   >  1:5083921/1‑139 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                                   >  1:729353/1‑140 (MQ=255)

      agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGAGTCTGTTTTTTTATTTTTGTTCTAATACTAAGTCGATCATAACGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAAAGGCTTTTc                                                                                                                                   >  2:1344092/1‑140 (MQ=255)

        agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATGGCTTTTCCg                                                                                                                                 >  2:3954306/1‑140 (MQ=255)

            ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTGTTACGtttt                                                                                                                             >  2:2201777/1‑140 (MQ=255)

             gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGttttt                                                                                                                            >  2:224994/1‑140 (MQ=255)

             gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTCCGttttt                                                                                                                            >  2:1663525/1‑140 (MQ=255)

                cATCTCTGATGTTGGAATTATTCAGAGCTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaaa                                                                                                                         >  2:1178144/1‑140 (MQ=255)

                cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGGTTTTCCGTTTTTaaa                                                                                                                         >  1:3545208/1‑140 (MQ=255)

                cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGGTCGGCTTTTTGTTTGAAGTTTTTGGAGATTTGTATGGGTTTTGAGTTTTTaaa                                                                                                                         >  1:4857363/1‑140 (MQ=255)

                   ctcttctGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTGCTCATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                      <  1:4037626/134‑1 (MQ=255)

                   ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                      <  1:1823028/140‑1 (MQ=255)

                    tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAaaaaaa                                                                                                                     >  2:5500980/1‑140 (MQ=255)

                            tGGAATTCTTCAGAGAAGCTGTTTTTGTATTTTTGTACTAATCCGAAGTCGATCATTCCGCCTATACGGGCGTTGTCGGATTTTTGATTGAAGTTTTGGGAGATTTGAATGGCTGTTCCGTTTTTAAAACAAGCGGCAAg                                                                                                             <  1:4706829/140‑1 (MQ=255)

                            tGGAATTATTCAGAGATGCTGTTTTTTTATTTTTGTTCTAATACGAAGTAGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  1:1281530/140‑1 (MQ=255)

                            tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  2:3776709/140‑1 (MQ=255)

                            tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  1:174288/140‑1 (MQ=255)

                            tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGGGAGCTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  1:2782826/140‑1 (MQ=255)

                             tgAATTATTCAGAGAGGCTGGTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGGAGTTTTGGGAGATTTGACTGGGTGTTCCGTTTTTTAACCAAGAGGCACGc                                                                                                            >  2:3225746/2‑140 (MQ=255)

                             ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                            >  1:4245362/1‑140 (MQ=255)

                                 ttattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGa                                                                                                        <  2:1811739/140‑1 (MQ=255)

                                   attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                      <  1:3844390/140‑1 (MQ=255)

                                           aTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTAGTTTTGGGAGATTTGAATTGCTTTTGCGTTTTTAAAACAATAGGCAAGCCAGCTAAAACCGac                                                                                              >  2:2077215/1‑140 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAACCGacaaa                                                                                           >  2:1007348/1‑139 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                           >  2:5335382/1‑140 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                           >  2:559314/1‑140 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGacaac                                                                                           >  2:3318904/1‑140 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                                 <  1:2019511/118‑1 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAATTTTTGGAGATTTGAAATGCTTTTCAGTTTTTAAAAAAACACGCAACCCACAAAAACCCCacaac                                                                                           >  1:620899/1‑140 (MQ=255)

                                              cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAGAAGAGGCAAGCCAGATAAACCCGacaac                                                                                           >  2:4376032/1‑140 (MQ=255)

                                                 tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTGTTTGAACTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATATGCAAGCCAGATAAAACCGacaacaaa                                                                                        >  1:441562/1‑139 (MQ=255)

                                                           tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                              >  2:300006/1‑140 (MQ=255)

                                                             tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                            <  1:5680071/140‑1 (MQ=255)

                                                             tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                            <  1:4660483/140‑1 (MQ=255)

                                                              gTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTAGTTTTGGGACATTTTAATGGCTGTTCCGGTTTTAAAACAAGATGCAAGGCAGATAAACCCGAAAACAACAAAAAATAATCTa                                                                           >  1:1003919/1‑140 (MQ=255)

                                                               ttCTACTTCGAAGTCGATCATTCCGACTATACGGGCTTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGATTTTCCGTTTTTAAAACAAGATGCAATCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:2967815/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:926786/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  2:1417661/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  2:4589828/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  2:934383/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:1663525/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:2163912/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:3949950/140‑1 (MQ=255)

                                                               ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTGTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:1138463/140‑1 (MQ=255)

                                                                    aTACTAAGTCGATCATTCCGACTATAAGGGCGTTTTCGGATTTTTGTTTGAAGGTTTGGGAGAGTTGAATTGCGTTTCCGTTTTTTAAAAAAGAGGCCAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCaact                                                                     >  2:2657182/1‑138 (MQ=255)

                                                                     tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                    <  2:4933991/140‑1 (MQ=255)

                                                                     tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                    <  1:322383/140‑1 (MQ=255)

                                                                     tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                    <  1:4341284/140‑1 (MQ=255)

                                                                     tACGAAGTCGATCATACCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGCATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACCACAACAAAAAATAATCTAGCAAGTa                                                                    <  1:802992/140‑1 (MQ=255)

                                                                     tACGAAGTCGATCACCGCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATGTGAATGGATTTTCCGTTTTTAAAACAAGTGGCAAGCCAGATAAACCCGACAACAACTAAAAATAATCTAGCAAGTa                                                                    <  1:3624424/140‑1 (MQ=255)

                                                                        gAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAAc                                                                 <  1:2301826/140‑1 (MQ=255)

                                                                         aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACAACAAAAAAAAATAATCTAGCAAGTaaaat                                                                >  2:420095/1‑138 (MQ=255)

                                                                         aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGGTTTGGGAGATTTGAATGGCTTTTGCGTTTTTAAAACAAGAGGCAATCCAGTTAAACCCGGCAACCACAAAAAATAATCGAGCCAGTAAACt                                                                >  2:5172322/1‑140 (MQ=255)

                                                                          aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                               <  1:5133626/140‑1 (MQ=255)

                                                                           gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                              <  1:2106005/140‑1 (MQ=255)

                                                                           gTCGATCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                              <  1:2225937/140‑1 (MQ=255)

                                                                               aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                                                          >  2:4981597/1‑140 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:3467048/140‑1 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:1844346/140‑1 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:3926713/140‑1 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:2915814/140‑1 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:5510351/140‑1 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTAGAATGGCTTTTCCGTTTTTAAACCAAGAGGCAAGCCAGATAAACCCGACAACAACAAAATATAATCTAGCAAGTAAACTCcatgca                                                         <  1:3774415/140‑1 (MQ=255)

                                                                                tCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:4134452/140‑1 (MQ=255)

                                                                                acATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:2729395/139‑1 (MQ=255)

                                                                                  aTTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgcatg                                                       <  2:5046042/140‑1 (MQ=255)

                                                                                      cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGt                                                   >  2:4524109/1‑140 (MQ=255)

                                                                                      cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGt                                                   <  2:794578/140‑1 (MQ=255)

                                                                                      cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGt                                                   <  2:1976603/140‑1 (MQ=255)

                                                                                            taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattca                                             >  2:5212246/1‑140 (MQ=255)

                                                                                             aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATAGCAAGTAAAAAGCATTCAAGGGGTAattcat                                            >  2:656747/1‑140 (MQ=255)

                                                                                                  cGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtt                                       >  2:1136398/1‑140 (MQ=255)

                                                                                                      ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                   <  1:3410619/140‑1 (MQ=255)

                                                                                                        cGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaaaa                                 <  1:4981597/140‑1 (MQ=255)

                                                                                                          gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                               <  2:2846453/140‑1 (MQ=255)

                                                                                                           aTTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAatat                              <  2:4809925/140‑1 (MQ=255)

                                                                                                           aTTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAatat                              <  2:736795/140‑1 (MQ=255)

                                                                                                                 ttttgAAGTTTTGGGAGATTTTAATGGATTGTCCGTTTTTAAAAAAAGAGGCATGACAGATAAACCCTACAACAACAAAAAATAATCTATCAAGTAAACTCCATGCATGGTTTAATTCATTCGTTGCAAAATATAAAAAt                        <  1:5239108/139‑1 (MQ=255)

                                                                                                                   ttgCAGTCTTGGGAGATGTGACTGGCTCTTCCGTTTTTAAAACAAGAGGCAAGCCAGCTAAACCCGACAACAACACAAAAGAATCGAGCAAGTAAACTCCATGCATGGGGTAAGTCATTCGTTGCAAAATATAAAAATag                      <  2:4311976/140‑1 (MQ=255)

                                                                                                                   ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                      <  1:1418217/140‑1 (MQ=255)

                                                                                                                    tgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATaga                     <  2:5730247/140‑1 (MQ=255)

                                                                                                                      aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                   <  2:3512712/140‑1 (MQ=255)

                                                                                                                      aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                   <  2:3114510/140‑1 (MQ=255)

                                                                                                                                ataTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTAGTTGCAAAATATAAAAATAGAGTTACTAAtgtg         <  2:3710627/138‑1 (MQ=255)

                                                                                                                                   tttGAATGGGTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACACGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATATAGTTACTAATGTGcct      >  2:1523383/1‑139 (MQ=255)

                                                                                                                                       aaTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGa  <  2:5113045/140‑1 (MQ=255)

AGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGA  >  NZ_CP034943/240073‑240347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`