Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 6.1% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA 6.1% 1149.3 / 6.2 466Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (266/151);  new base A (2/47);  total (268/198)
Fisher's exact test for biased strand distribution p-value = 4.80e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

GTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCA  >  NZ_CP034943/316125‑316399
                                                                                                                                         |                                                                                                                                           
 tgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCaaa                                                                                                                                         >  1:967011/1‑140 (MQ=255)
                gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                         >  1:4601983/1‑140 (MQ=255)
                 tgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaaa                                                                                                                        <  2:1633301/140‑1 (MQ=255)
                  gtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCCGTTCAAATGCTTGCAAACGGAAAg                                                                                                                       >  2:4287296/1‑140 (MQ=255)
                   ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                      >  1:3780262/1‑140 (MQ=255)
                     aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                    >  1:1011553/1‑140 (MQ=255)
                     aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                    >  1:2781270/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                              >  1:528387/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                              >  2:4202921/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                              >  1:2848550/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGACAACGGAAAGACaaaaaaa                                                                                                              >  2:1764621/1‑140 (MQ=255)
                             aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                            <  1:5396616/140‑1 (MQ=255)
                                 aTTTTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAATATAAAAGTCGATAAAGTATCTAAAATTTTTGGGAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATc                                                                                                        <  1:697577/140‑1 (MQ=255)
                                 aTTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATc                                                                                                        <  1:4177376/140‑1 (MQ=255)
                                  ttGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATcc                                                                                                       >  1:3414587/1‑140 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                      >  1:5443819/1‑140 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                      >  1:2348815/1‑140 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                      >  2:484080/1‑140 (MQ=255)
                                     tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                    >  1:1346362/1‑140 (MQ=255)
                                          tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAACGTCAAAAAAAGAGATCCTGAAAGCg                                                                                               >  2:4116403/1‑140 (MQ=255)
                                          tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCg                                                                                               >  1:3330450/1‑140 (MQ=255)
                                          tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCg                                                                                               >  2:4537966/1‑140 (MQ=255)
                                            tggtggAAAAATTTTTGTGTATTTTAGAAACCAATTAAGATAAAATTCGAGAAAGTATATAAAATTTTTGGCAAAAAAAAAAACAAACCAGTTAAAATGCTTGCCAACGCAAACACAAAAAAAGATATCCTGAAAGCGAc                                                                                             <  2:3537256/140‑1 (MQ=255)
                                            tGGAGGTAAAATATTATTGTAGATTATAACCAAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAAAAAAGAAAGAAGTTCAAAGGCTTGCAAACGGAAAGACAAAAAAAGAGAACCTGAAAGCGAc                                                                                             <  2:1087336/140‑1 (MQ=255)
                                            tGGAGGGAAAATATGTGTGTAGATGAGAAACAAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGATTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGAc                                                                                             <  2:2912298/140‑1 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                             >  1:2042244/1‑91 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  2:5088776/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGCAAGACCAAAAACGAGCTCCTCAAAGCGACCgg                                                                                          >  2:3927915/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACACAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:1087812/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:5719121/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:164151/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:366334/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:3203033/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGAGAcctg                                                                                          >  1:1900280/1‑138 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGCTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  2:5610706/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGAGGAGAAACCAATTAATATAAAAGTCGAGAAAGTCTCTAAAATATTTGGGAAACAAACAAAGAAAGAAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCAAACgg                                                                                          >  1:4366252/1‑140 (MQ=255)
                                                 ggAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGAt                                                                                        >  2:2400925/1‑140 (MQ=255)
                                                  taaaaTATGAGGGTAGATGAGAAACAAATTAATATAAAAGTCGATAAAGTATATAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATc                                                                                       <  2:3993342/139‑1 (MQ=255)
                                                  gAAAATATGAGTGTAGATGATAAACCAATTAAGATAAAAGTCGAGAAAGTATATAAATTTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTCCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATc                                                                                       <  2:4315150/140‑1 (MQ=255)
                                                  caaaaTATTCGTTTAGTTGCCAAACCAATTAAGATCAAAGTCGCGAAATTATATAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCCAGGTAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATc                                                                                       <  2:730320/139‑1 (MQ=255)
                                                        atGAGTTTAGATGATAAACCAATTAAGATAAAAGTCGATAAATTATCTAAAATTTTTGGCAAACAAACAAAGAACGCAGTTCAAAGGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGt                                                                                 <  2:5719121/140‑1 (MQ=255)
                                                             tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                            >  1:2223264/1‑140 (MQ=255)
                                                             tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                            >  1:3534213/1‑140 (MQ=255)
                                                               tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTGGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                          >  1:1248300/1‑140 (MQ=255)
                                                                      taaaaaaaTTAATATAAAAGTCTATAAAGTATATAAAATTTTTGGCAAACAAACAAAGAAAGCAGTAAAAATGGTTGCCAACGCAAAGACAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAg                                                                   <  2:5239768/134‑1 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTTCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:2976016/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:4094890/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGc                                                                  >  2:5001312/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGTAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  2:1412190/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:4735120/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  2:554007/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:4976103/1‑140 (MQ=255)
                                                                        aaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATc                                                                      >  1:2536337/1‑135 (MQ=255)
                                                                        aaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATc                                                                      <  2:2536337/135‑1 (MQ=255)
                                                                         aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                >  1:1741216/1‑140 (MQ=255)
                                                                            aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                             >  2:822523/1‑140 (MQ=255)
                                                                            aaTAAAGATAAAATTCGAGAAATTATCTAAAATTTTTGGGAAAAAAACAAACAAACCAGTACAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                             <  2:1157308/140‑1 (MQ=255)
                                                                               tAATATAAAAGTCTAAAACTTATATAAAATTTTTGGCAAACAAACAATTAAACCATTTCAAATGCTGCCCACCGCAAACACAAAAAAAGCGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  1:557164/140‑1 (MQ=255)
                                                                               tAAGATAAAACTCGAGACAGTAAATAAAATGTTGGGCAACAAAACAAAGAAAGAATTACAAATGCTGCCCAACGCAAACAAAAAAAAAGAGACCCTCACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  1:1450374/140‑1 (MQ=255)
                                                                               tAAGATAAAACTCGAGAAATTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGCAAACACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  2:3875373/140‑1 (MQ=255)
                                                                               tAACATAAAAGTCGACAAAGTATCTAAAATTTTTGGCAAACAAACAAACAAAGCAGTACAAATGCTGCCAACCGCAAACACAAAAAAAGACATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  1:484080/140‑1 (MQ=255)
                                                                                 ataaaaaaGTCGATAACTTATCAAAATTTTTTGGCAAAAAAAAAAACAAACCTTTCAATATGTTTCCCACCGCAAAGAAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  1:578266/136‑1 (MQ=255)
                                                                                 ataTAAACGTCTAGAAAGTATCTAAAATTTTTGGCAAAAAAAAAAATAAAGCAGTTAAAATGCTTGCCACCGGAAACAAAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:5125380/138‑1 (MQ=255)
                                                                                 aGATAAAAGTCGACAAAGTATCAAAATTTTTTGGTAAACAAACAAAGAACCCAGTTAAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:4892600/140‑1 (MQ=255)
                                                                                     aaaaGTAGAGAAATTATCTAAAATTTTTGGGAAACAAAAAAAGAAATAATTTCAAATTATTGCAAACGTAAAGAAAAAAAAACATATAATGAAAGCGACCTGATCAACCTTTGGGTTAAATCAATCAGATTTTGAttttt                                                    >  2:4090615/1‑136 (MQ=255)
                                                                                      aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                   <  2:640534/140‑1 (MQ=255)
                                                                                         ttagataaaTTATATAAAATTTTTGGAAAAAAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGTAAATAAAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatga                                                <  2:3289637/134‑1 (MQ=255)
                                                                                           cGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatgatg                                              >  1:3862620/1‑140 (MQ=255)
                                                                                            gacaaaGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                             <  2:3203033/137‑1 (MQ=255)
                                                                                             acacattATCTAAATTTTTTGGGAAAAAAAAAAATAATGCATTTAAAATGCTTGCCAACGTAACGAAAAAAAAATAGATCCTAAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                            <  1:405200/134‑1 (MQ=255)
                                                                                               aaaGTATATAAAATTTTTGGCAAAAAAAAAAAGAAAGCAGTTAAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTga                                          <  1:4552788/140‑1 (MQ=255)
                                                                                                 attATCTAAAATTTTTGGGAAACAAAAAAACAACCCTGTTCAAAGGCTTGCCAACGTAAACACAAAAAAATACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgaga                                        <  2:2052488/138‑1 (MQ=255)
                                                                                                 aGTATCTAAAATTGTTGGCAAACAAAAAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgaga                                        <  2:5377056/140‑1 (MQ=255)
                                                                                                      atAAATTTTTTGGGAAAAAAACAAACAACGCATTTAAAAGGGTTGCCAACTCAAATAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                   <  1:3265348/139‑1 (MQ=255)
                                                                                                      atAAATTTTTTGGCAAAAAAAAAAAGAAAGCTTTTCAATTGCTTGCAAACGTAAACAAAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                   <  1:534076/139‑1 (MQ=255)
                                                                                                            tttttGGGAAACAAACAAAGAAAGCCGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                             <  2:1035067/140‑1 (MQ=255)
                                                                                                             ttttGGTAAAAACACAAAGCTACCACTTATATTGATTGCCAACCCAAATAAAAAAAAAGAGCTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATCTGATGGTGAGATATTTGtcatca                            <  2:4395652/140‑1 (MQ=255)
                                                                                                             ttttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                            >  2:1817591/1‑140 (MQ=255)
                                                                                                             ttttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                            >  2:4680811/1‑140 (MQ=255)
                                                                                                              tttGGTAAAAAAACAAATAAAGCTGTACAAATGCTTGCAACCGGAAAGAAAAAAAATGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  1:3915366/140‑1 (MQ=255)
                                                                                                              tttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTGGCCAACGGAAAGACAAAAAAAGAGAGCATGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  2:371585/140‑1 (MQ=255)
                                                                                                              tttGGCAAACAAACAAACAAAGCATTTCAAAGGCTTGCCAACGTAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  1:4882459/140‑1 (MQ=255)
                                                                                                              tttGGCAAACAAACAAACAAACCTGTCAAAATCCTTCCAAACGCAAACACAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  1:832235/140‑1 (MQ=255)
                                                                                                              tttGGCAAAAAAACAAAGAACGCATTTCAATTGCTTCCCAACGCAAACAAAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  1:427373/140‑1 (MQ=255)
                                                                                                              tttGCGAAAAAAACAAAGAAAGATGTTAAACTGCTTGCAAACGCAAACACAAAAAAAGATAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  1:3112357/140‑1 (MQ=255)
                                                                                                              tgtGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGACCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  2:3043380/138‑1 (MQ=255)
                                                                                                               ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTCAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                          >  2:2152407/1‑140 (MQ=255)
                                                                                                               ttGGCAAACAAACAAACAAAGCCCTTCAAAGGCTGCCCACCGCAAACACAAAAAAAGAGAGCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                          <  1:5088776/140‑1 (MQ=255)
                                                                                                                tGGCAACCAAACAAACAACCCCGGTCAAATGCTTCCCAACGCAAAGACAAAAAAAGAGATCCTGACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                         <  2:3414587/140‑1 (MQ=255)
                                                                                                                tGGCAAAAAAAAAAACAACCCATTTCAAATGCTTGCCAACGTAAAGAAAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                         <  1:467435/140‑1 (MQ=255)
                                                                                                                  ggAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                       <  2:366334/140‑1 (MQ=255)
                                                                                                                  ggAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                       <  2:2811863/140‑1 (MQ=255)
                                                                                                                  ggAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                       <  2:3780262/140‑1 (MQ=255)
                                                                                                                  gcaaacaaacAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                       <  2:3812729/138‑1 (MQ=255)
                                                                                                                      aaaaacaaaGAAAGCAGTTCAACTGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTAt                   <  2:541093/138‑1 (MQ=255)
                                                                                                                           aaaagaaagCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg              <  2:59294/139‑1 (MQ=255)
                                                                                                                           aaaagaaacCAGTTCAAATTCTTGCAAACGGAAAGAAAAAAAAACATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg              <  2:1973258/139‑1 (MQ=255)
                                                                                                                                 aagaagTTCAATTGCTTGCCAACGGAACCAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGt        <  1:3968887/140‑1 (MQ=255)
                                                                                                                                  agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa       >  2:2659300/1‑140 (MQ=255)
                                                                                                                                      gTTCAAATGCTTGCCAACGGAAAGAGAACAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGATGTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAACTc   >  1:5137063/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCA  >  NZ_CP034943/316125‑316399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: