Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 485,053 G→T 5.9% L445F (TTG→TTT kimA → potassium transporter KimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943485,0530GT 5.9% 1019.3 / 8.8 415L445F (TTG→TTTkimApotassium transporter KimA
Reads supporting (aligned to +/- strand):  ref base G (162/213);  new base T (38/2);  total (200/215)
Fisher's exact test for biased strand distribution p-value = 5.70e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

AAACCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAAT  >  NZ_CP034943/484916‑485186
                                                                                                                                         |                                                                                                                                     
aaaCCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCt                                                                                                                                     >  2:883015/1‑140 (MQ=255)
aaaCCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCt                                                                                                                                     >  2:5111291/1‑140 (MQ=255)
aaaCCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCt                                                                                                                                     >  2:5028441/1‑140 (MQ=255)
     aaaaGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGtttt                                                                                                                                >  2:4575715/1‑140 (MQ=255)
       aaGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCGTGTGTTAATATTTATGCCTATCGGCGTTTTGCTGtttttt                                                                                                                              >  1:1049218/1‑140 (MQ=255)
       aaGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTGCTGtttttt                                                                                                                              >  1:2682680/1‑140 (MQ=255)
            tGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGAt                                                                                                                         >  2:1879704/1‑140 (MQ=255)
                tCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaaa                                                                                                                     <  2:1817932/140‑1 (MQ=255)
                tCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaaa                                                                                                                     <  1:2399275/140‑1 (MQ=255)
                tCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaaa                                                                                                                     <  1:546856/140‑1 (MQ=255)
                tCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaaa                                                                                                                     <  1:3054071/140‑1 (MQ=255)
                 cGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaaaa                                                                                                                    <  1:4667171/140‑1 (MQ=255)
                    aaaaaTGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATc                                                                                                                 <  2:5346695/140‑1 (MQ=255)
                     aaaaTGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCa                                                                                                                <  2:1986460/140‑1 (MQ=255)
                          gCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTaca                                                                                                           <  1:116491/140‑1 (MQ=255)
                              aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                       <  2:2788987/140‑1 (MQ=255)
                              aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGAGTAAAAATCAATACACAGc                                                                                                       >  2:4970740/1‑140 (MQ=255)
                              aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGGGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACAAaacaac                                                                                                       >  1:4490100/1‑138 (MQ=255)
                               tCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCt                                                                                                      <  1:5113532/140‑1 (MQ=255)
                                   cTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCg                                                                                                  >  2:3038089/1‑140 (MQ=255)
                                   cTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCg                                                                                                  <  1:3774041/140‑1 (MQ=255)
                                   cTCCTGCGGCGCTGTGCTGGCGATGATGGGACTCTCGATCCGGGTTGTGACGCCGTTTAATGTTGTATGGCCTGTGTTAATCTTTATCCCTCTCGCCGATTTGCTGTTTCTTGCGAGTCAAAATCACTACACAGCTGTCg                                                                                                  <  2:3340171/140‑1 (MQ=255)
                                      cTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTg                                                                                               >  2:1578212/1‑140 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                             <  1:1369737/140‑1 (MQ=255)
                                                   aTTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCAt                                                                                  <  1:355492/140‑1 (MQ=255)
                                                   aTTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTAAGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCAt                                                                                  <  2:1404439/140‑1 (MQ=255)
                                                       cGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTa                                                                              <  1:2021413/140‑1 (MQ=255)
                                                           tATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACa                                                                          <  2:3731177/140‑1 (MQ=255)
                                                           tATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACa                                                                          <  2:616751/140‑1 (MQ=255)
                                                            aTGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTCTGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACaa                                                                         <  1:1446270/140‑1 (MQ=255)
                                                            aTGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCATATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGTTGAACAGCTTCGCATCGTAGACaa                                                                         <  2:1296454/140‑1 (MQ=255)
                                                                 tCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGc                                                                    <  1:1231743/140‑1 (MQ=255)
                                                                  cTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTAAACACATGTCGGTTAACAGCATAGCATCGTATACAAAGAGcc                                                                   >  1:2776845/1‑140 (MQ=255)
                                                                  cTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCAATACACATCTGTCGGTGAACAGCTTCGCATCGTAGACAAATAGcc                                                                   >  2:4078814/1‑140 (MQ=255)
                                                                          ccggTTTGTGACGAAGTTTAATGGTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCCCAACGTAGACAAAGAGCCAGAAGaaa                                                           >  2:2348803/4‑140 (MQ=255)
                                                                          ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTAGCTGTTTTTTGCGATTAAAAATCACTACAAAGCTGTCGCTGAACAGCTTCCCATCGTAGACAAAGAGCCCGAACaaa                                                           >  1:2327064/1‑140 (MQ=255)
                                                                           cTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAAt                                                          <  1:2160156/140‑1 (MQ=255)
                                                                            ggTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATc                                                         >  2:2644128/2‑140 (MQ=255)
                                                                               ttGTGACGAAGTTTCATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGGTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTAGCATCGTATACAAAGAGGCAGAAGAAATCaaa                                                      >  1:36675/1‑140 (MQ=255)
                                                                                  tgACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGc                                                   <  1:3596678/140‑1 (MQ=255)
                                                                                  cgccGAAGTTTCATGTTGCCGGCCCTCTGTTAAGCGTTAGCCCTATCGTCGTATGGCTGTTTTCTGCGATTAAAAATCACTACACCGCTTTCGCAGAACAGCTTCGCATCGTAGACAAAGAGCCAGCAGAAATCAAAGGc                                                   <  2:3891167/137‑1 (MQ=255)
                                                                                   gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTAAACAGCAGTCGGTGTACAGCATCGCATCGTAGACAAAGAGCCAGAACAAAAAAAAGGCa                                                  >  1:3511844/1‑140 (MQ=255)
                                                                                   gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                                  <  1:4560583/140‑1 (MQ=255)
                                                                                    aCGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCAc                                                 >  2:5590878/1‑140 (MQ=255)
                                                                                    aCGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCAc                                                 <  1:5067231/140‑1 (MQ=255)
                                                                                       aaGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAATATAAATACACAGCTGTCGGTGAACAGCTTGGCATAGTAGACAAAGATCCAGAAGAAATCAAAGGCAAGGt                                              >  1:4970116/1‑140 (MQ=255)
                                                                                        aGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATGGTAGACAAAGAGACAGAAGAAATCAAACGCACGGtt                                             >  2:5472237/1‑140 (MQ=255)
                                                                                        aGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGtt                                             <  2:5648931/140‑1 (MQ=255)
                                                                                             aaTGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGAt                                        <  2:3591732/140‑1 (MQ=255)
                                                                                              aTGTTGTATGTCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGAtt                                       <  2:4448924/140‑1 (MQ=255)
                                                                                              aTGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCTATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCACAAGAAATCAAAGGCACGGTTGTGAtt                                       >  2:1173614/1‑140 (MQ=255)
                                                                                              aTGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTATAAAAAGAGACAGAAGAAATCAAAGGCACGGTTGTGAtt                                       >  2:5776634/1‑140 (MQ=255)
                                                                                              aTGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGAtt                                       >  2:2553802/1‑140 (MQ=255)
                                                                                               tgttgtATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATtg                                      <  1:4012294/140‑1 (MQ=255)
                                                                                                   gtATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGcc                                  <  2:1659820/140‑1 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTCTCGTCGTTTTGCTGTTTTTTGAGATGAAAAATAACTAAACAGCTTTCGGTGAACAGCTTAGCATCGTAGAACAAGATCCAGAAGAAATCAATGGCCCGGTTGTGTATGTGGCt                                 >  1:703900/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATAACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCt                                 >  1:1604641/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTTTTTTTTGCGATTAAAAATCACTACCCAGCTGTAGGTGACCAGCTTCGCATCGTAGACAAAGAGCCAGAAGCAATAAAAGGCCAGGGTGTTATTTTGGCt                                 >  1:3932/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACAAAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAACGCACGGTTTTGATTGTGCCt                                 >  1:5750582/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTGCTGTTTTTTGCGATTAAAAAAAACTACACACCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGATCCAGAAGAAATAAAAAGCCCCGTTGTGATTGTGCCt                                 >  1:3272484/1‑140 (MQ=255)
                                                                                                         ccTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGc                            >  2:3234294/1‑140 (MQ=255)
                                                                                                         ccTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAAACAAACGCACGGTTGTGATTGTGCCTGTAGc                            >  2:5237891/1‑140 (MQ=255)
                                                                                                          ctttgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:2998139/137‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAGGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:5357276/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  2:2732269/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:1097371/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:1017290/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:521455/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:3225813/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:3449145/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:3094275/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:4161695/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:4914611/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:2078626/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:5532077/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:835024/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                           <  1:1706052/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGAGTGTGCCTGTAGcc                           <  1:561064/140‑1 (MQ=255)
                                                                                                           tgtgTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCg                          <  2:399565/140‑1 (MQ=255)
                                                                                                           tgtgTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCGTCGCAGCGTAGACAAAGAGCCCGAAGAAATCCAATGCACGGTTGTGATTGTGCCTGTAGcgg                          >  1:4804390/1‑138 (MQ=255)
                                                                                                            gtgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTTTGCCTGTAGCCgg                         >  2:770546/1‑140 (MQ=255)
                                                                                                             tgtTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGCTTAAAAATCACTAAACAGCTGTCGGTGAACAGCTTCGCATCGTAGAAAAATAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTATCCGgt                        >  1:2924277/1‑140 (MQ=255)
                                                                                                             tgtTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACCAAGCTGGCGGTGAACAGCTTAGCAACGTAGACAAAGAGCCACAAGAAATCAAAGGCACGGTTGTGATTGTTCCTGTAGCCGgt                        >  2:2929809/1‑140 (MQ=255)
                                                                                                                  aTCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTtac                   >  2:3135345/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATCACTAAACACCTGTCGGTGAACAGCTTCGCAACGTAGACAAAAAGCCAGAAGAAATCAAAGGCACCGTTTTGATTTTGCCTGTAGCCGGTGTTACTACcg              >  1:5304421/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCAc                                                 >  1:4353062/1‑105 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTTTTTTTGCCTGTAGCCGGTGTTAATACcg              >  2:2416655/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGc                            >  1:2831779/1‑126 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg              >  1:2586413/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACAAAGCTGTCCGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAACTCAAAGCCAAGCTTGTTATTTTGCCTGTAGCCGGTGTTACTACcg              >  2:1074321/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAACTACACAGCTGTCGGTGAACAGCTTCTCATCGTAGACAAAGAGACAGAAGAAATCAAAGTCACGGTTGTGATTGTGACTGTATCCGGTCTTACTACcg              >  2:3152755/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGGTACTACcg              >  2:4181154/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTGTTTGTGTTTTTTGCGATTAAAAATGACTAAACATCTGTCGGTGAACAGCTTCGCAACGTAGACAAAGAGCCAGAAGAAAAAAAAGGCACGGGTTTGTTTTTGGCTGTAGCCGGTGTTACTAAcg              >  1:5152853/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTGTTGCTGTTTTTTTCGATTAAAAATCACTACACAGCTGTCGGTGTACAGCTTCGCATCGGATACAAATAGCCAGAAGAAATCAAAGGCACGGTTTTTATTGTGCCTGTAGCCGGTGTtaagacag              >  1:1878577/1‑134 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTGTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGGAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg              >  1:910286/1‑140 (MQ=255)
                                                                                                                         tGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcgtc            >  2:2289953/1‑140 (MQ=255)
                                                                                                                              aTCGTCGTTTTTCTGTTTTTTGCGATTAAAAATAACAACACAGCTGTCGGTTAACAGCATAGCATCGTAGAAAAATAGGCAGAAGAAATCAAATGCAAGGTTTTGATTGTGGCTGTATCCGGTGTTAATACCCTCGTTCa       >  1:2454765/1‑140 (MQ=255)
                                                                                                                               tcgtcgTTTTTCTGTTTTTTGCGCTTAAAAAAAACTACACAGCATTCGGTGAACAGCATAGCATCGTAGAAAAATAGCCAGAAGAAATAAAAGGCACGGTTGTGATTTTTCCTGTAGCCGGTGTTACTACCGTCGTGCaa      >  1:1550210/1‑140 (MQ=255)
                                                                                                                               tcgtcgTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGATCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCaa      >  1:4436179/1‑140 (MQ=255)
                                                                                                                                   cgtTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAAt  <  1:3823083/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
AAACCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAAAAAT  >  NZ_CP034943/484916‑485186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: