Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,057 C→A 7.1% F23L (TTC→TTA EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,0570CA 7.1% 977.9 / 9.5 360F23L (TTC→TTAEO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (174/147);  new base A (2/37);  total (176/184)
Fisher's exact test for biased strand distribution p-value = 6.16e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

AACTGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTG  >  NZ_CP034943/548918‑549195
                                                                                                                                           |                                                                                                                                          
 aCTGGAAGTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCa                                                                                                                                           <  1:4359546/140‑1 (MQ=255)
  cTGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCaa                                                                                                                                          <  1:2467087/140‑1 (MQ=255)
   tGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCaaa                                                                                                                                         <  2:2241510/140‑1 (MQ=255)
    ggAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAg                                                                                                                                        <  2:1051513/140‑1 (MQ=255)
    ggAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAg                                                                                                                                        <  2:1603605/140‑1 (MQ=255)
    ggAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTGAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAg                                                                                                                                        <  2:3499170/140‑1 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                       >  1:5748318/1‑140 (MQ=255)
     gAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGa                                                                                                                                       >  2:3037880/1‑140 (MQ=255)
        ttGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGaaaa                                                                                                                                    <  2:2162633/140‑1 (MQ=255)
         tGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAAc                                                                                                                                   <  1:92021/140‑1 (MQ=255)
          ggCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                  <  1:4792699/140‑1 (MQ=255)
          ggCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                  <  1:4668310/140‑1 (MQ=255)
          ggCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                  <  1:1750254/140‑1 (MQ=255)
          ggCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                  <  1:783486/140‑1 (MQ=255)
          ggCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACa                                                                                                                                  <  2:2290354/140‑1 (MQ=255)
           gCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGACATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACAt                                                                                                                                 >  1:5429/1‑140 (MQ=255)
            ccTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATg                                                                                                                                <  1:5046702/140‑1 (MQ=255)
                      aCAGCCAGTTTTCACAAAAAAATGCATACCAGTTTGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGc                                                                                                                      <  2:2443549/140‑1 (MQ=255)
                      aCAGCCAGTTTTCACAAAAAAATGCATACCAGTTCGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGc                                                                                                                      >  1:1044005/1‑140 (MQ=255)
                       cAGCCAGTTTTCACAAAAAAATGCATCCCAGTTAGGAGAACAACCCAAATTGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCg                                                                                                                     <  2:1514119/140‑1 (MQ=255)
                        aGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGt                                                                                                                    <  1:1154966/140‑1 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                  >  1:2202322/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                  >  2:5529348/1‑140 (MQ=255)
                          ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                  >  1:3881435/1‑140 (MQ=255)
                               tttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaa                                                                                                             <  2:801888/140‑1 (MQ=255)
                                ttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaaa                                                                                                            >  1:322767/1‑140 (MQ=255)
                                       aaaaaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATg                                                                                                     <  2:524594/140‑1 (MQ=255)
                                          aaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAg                                                                                                  <  1:2139461/140‑1 (MQ=255)
                                          aaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAg                                                                                                  <  2:1158064/140‑1 (MQ=255)
                                             gCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                               <  2:2755224/140‑1 (MQ=255)
                                               aTACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTccc                                                                                             <  1:2792290/140‑1 (MQ=255)
                                                 aCCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCtt                                                                                           >  1:1015044/1‑140 (MQ=255)
                                                             aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCACAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                               >  2:279529/1‑140 (MQ=255)
                                                             aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                               >  2:3632289/1‑140 (MQ=255)
                                                              acaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTccg                                                                              <  1:4915392/140‑2 (MQ=255)
                                                              acaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCt                                                                              <  2:5084081/140‑1 (MQ=255)
                                                               caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                             >  2:4220250/1‑140 (MQ=255)
                                                               caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCGTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                             >  2:3135543/1‑140 (MQ=255)
                                                                aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                            >  1:5092918/1‑140 (MQ=255)
                                                                  cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                          >  2:4804661/1‑140 (MQ=255)
                                                                  cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                          >  2:907496/1‑140 (MQ=255)
                                                                     aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                       <  1:5529348/140‑1 (MQ=255)
                                                                     aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                       <  2:5626016/140‑1 (MQ=255)
                                                                       aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                     >  1:2174601/1‑140 (MQ=255)
                                                                            tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                >  1:4966566/1‑140 (MQ=255)
                                                                             atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTTGCGAGTCAGCTTTc                                                               >  1:4195160/1‑140 (MQ=255)
                                                                             atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                               >  2:3346727/1‑140 (MQ=255)
                                                                               cACGGCTTTTTTGAATAGATATCTGGATATTCCGGTTGATCAGCAGCCTGATCGCTAGTTCAAAGAATACCTGCTGCAGGGGCGTATTCAAACAGGAATGAAGCTCCGTTCCAAAAGCCTCCTGGCGAGTCAGCTTTCCa                                                             <  2:1395896/140‑1 (MQ=255)
                                                                               cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                             >  1:181622/1‑140 (MQ=255)
                                                                                aCGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAt                                                            >  1:4830419/1‑140 (MQ=255)
                                                                                    cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                        >  1:5627716/1‑140 (MQ=255)
                                                                                    cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                        >  2:4088224/1‑140 (MQ=255)
                                                                                    cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                        >  2:2652852/1‑140 (MQ=255)
                                                                                       ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCaa                                                     >  1:4607894/1‑140 (MQ=255)
                                                                                         ttttaTACAAATGTGTATATTCCGCTTTATCAGCAGATTTATCGATATTACAAACAAAACATCCATCAGGGGCGTTTTAAAAAAGCAATGACGCGCCCTTCCAAAAGACTCCGGGCGAGTAAGCTTCCCATCAGTCacac                                                   <  2:537294/136‑1 (MQ=255)
                                                                                         tttaaTATAAATCTGTATATTCCGCTTTATCAGCAGCTTTATCTATATTTCAAAGAAAACATGCATCCGGGGCTTTTTAAAAAAGTAATGAAGCGCCCTTCCAAACCACTCGTGGCGAGTCTGCTTTCCATCAGACAAAc                                                   <  2:5451606/137‑1 (MQ=255)
                                                                                         ttcaaTCGAAATCTGTATATTCCGCTTTATCAGCCGCTTTATCGCTATTTCAAACAAAACATCCATCCGGGGCTTTTTCAAAAAGGATTGAAGCTCCCTTCCAAAACACGCCTGGCGAGTCAGGTTTCCATCAGTCacac                                                   <  2:2115665/137‑1 (MQ=255)
                                                                                         ttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGGGTATTAAAAAAGGAATGAAGCTCCCTTCCAAACCACTCCTGGCTTGTCAGCTTTCCATCCGTCAAAc                                                   <  2:68668/140‑1 (MQ=255)
                                                                                              tAGAAAGCTGGATAGCCCGCTTTATCCGCTGCTTTAGCGCTATTTCAAAGAAAAACGGCATCAGGGGCGTATTAAAAAAGGAATCAAGCCCCCTACCAAACGACTCCTGGCGAGTCAGCTTTCCATAAGGCAAACGACTg                                              <  2:5106588/140‑1 (MQ=255)
                                                                                                taaaTCTGGATATTCCGCTTTATCAGAAGCTTTATCGATATTTCAAAGAAAACATGAATCAGGGGCTTTTTAAAAAAGTAATCAAGCGCCCTTCAAAAACACTCCTGGCCAGACAGCTTTCCATCCGTCACACGACTGTa                                            <  2:3230215/139‑1 (MQ=255)
                                                                                                aaaaTCTGTATATTACGCTTTATCAGCAGCTTTATCGATATTTCAAACAAAACATCAAACAGGGGCTTATACAAAAAGGAATTAAGCTCCCTTCAAAAACACTCCTGGCGAGTCAGCTTTCCAACAGACAAACGACTGTa                                            <  2:3937742/139‑1 (MQ=255)
                                                                                                     ttgtatatTCCGATTTATCAGCAGCTTTATCGATATTAAAAACAAAACATGAATCCGGGGCTTATTCAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCGGGCGAGTAAGCTTTCCATCAGACACACGACTGTAGAACg                                       <  1:2361690/136‑1 (MQ=255)
                                                                                                     cTGTATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAACAAAACATGCATCAGGGGCGTATTAAAAAAGTAATTAAGCGCCCTTCCAAACCACTCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACg                                       <  2:4963277/140‑1 (MQ=255)
                                                                                                     cTGTATATTCCGCTTTATCAGCAGATTTATCGATATTTCACATAAAACATGCATCGGGGGCGTATTCAAAAAGCAATCACGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACg                                       <  1:720507/140‑1 (MQ=255)
                                                                                                     cTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATACAAAAAGGAATGAAGCTCCCTCCCAAACGACCCCTGGCGAGTCAGCTTGCCATCCGTCAAACGACTGTAGAACg                                       <  2:4349322/140‑1 (MQ=255)
                                                                                                       tgATATTCCTCTTTACACTAAGATTTATCTATATTAAAAATAAAACTTTATTCAGGTGCTTATAAAAAATGTAATGAACCTCCGTACCAAAAGCCGCGTGCCTAGTCAGCTTTACACCAGTAAAACGACTGTAGAACGTg                                     <  2:1771758/139‑1 (MQ=255)
                                                                                                       ggATATTCCGCTTTATCAGCAGATTTAGCGCTATTACAAAGAAAACAGGCATCAGGGGCGTATAAAAAAAGGAATGAAGCTCCCTACCAAAACACTCCGGGCTAGTCAGCTTTCCAACAGTCACACGACTGTAGAACGTg                                     <  2:1565904/140‑1 (MQ=255)
                                                                                                        gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                    >  1:1377880/1‑140 (MQ=255)
                                                                                                             tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                               >  1:840499/1‑140 (MQ=255)
                                                                                                               ctttttATCAGCCGCTTTATCGATATTTCAAATAAAACAGGCATCAGGGGCTTATTCAAAATGCATTGAAGCTCCCTTCAAAACGACTCCTGGCGTGTCAGCTTTCCAACAGTCACACGACTGTAGAACGTGCCTATGaa                             <  1:2652852/137‑1 (MQ=255)
                                                                                                               cGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaa                             >  1:3596814/1‑140 (MQ=255)
                                                                                                                  tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                          >  1:5158443/1‑140 (MQ=255)
                                                                                                                  tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                          >  1:2882313/1‑140 (MQ=255)
                                                                                                                  tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                          >  2:4143112/1‑140 (MQ=255)
                                                                                                                     aTCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCtt                       >  1:518748/1‑140 (MQ=255)
                                                                                                                      tCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACGCCTGGCGAGTCAGCTGTCCATCAGTCAAACGACTGTAGAACGTGCCTCTGTACAGCTtg                      >  2:340492/1‑140 (MQ=255)
                                                                                                                      tCAGCAGCTTTATCGCTATTTCAAAGAAAACATCCATCAGGGGCTTATAAAAAAAGGAATGAAGCCCCCTTCCAAAAGACTCCTGGCGAGTCAGGTTTCCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTtg                      <  2:732713/140‑1 (MQ=255)
                                                                                                                         gcagcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtgctg                   >  2:4568140/1‑140 (MQ=255)
                                                                                                                         gcagcTTTACCGCTATTTCAACGAAAACAGGAATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCACAAGACGCCTGGCGAGTCAGCTTGCCCTCAGTAAAACGACTGTAGAACGTGCCTATGAACAGCTtgctg                   <  2:4757031/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGCTTTTTAAAACAAAACAGCCATCAGGGGCGTATACAAAACGGAATGAAGCTCCTTTCCAAAACACGCCTGGCGAGTCAGCTTTCCAACAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                 <  1:3993172/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGCTATTTCAAAGAAAACAGGCATCCGGGGCGTATAAAAAAAGGAATGAAGCTCCCTACCAAACGACGCCTGGCGAGTCAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                 <  1:5489757/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGATATTTCAAAGAAAACATGCATCCGGGGCGTATTCAAAAAGGAATGAAGCTCCCTGCCAAACGACGCCTGGCGAGGCAGCTTACCACCCGTAAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                 <  2:1736030/140‑1 (MQ=255)
                                                                                                                           agcTTTATCGATATTTAAAACAAAACATGCATCAGGGGCTTATAAAAAAAGCAATCAAGCTCCCTACAAAAACACACCTGGCGAGTCAGCTTTCCAACAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                 <  1:4309229/140‑1 (MQ=255)
                                                                                                                           agcTTTACCGCTATTTCAAACAAAACAGGCATCCGGGGCTTATTCAAAAAGGAAGCACGCGCCCTTCCAACACCCCCCGGGCGAGTCCGCTGACCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                 <  1:3093419/140‑1 (MQ=255)
                                                                                                                           agatttATCGCTATTTAAAACAAAAAATGCATCCGGGGCGTATTCAAAAAGGATTGAAGCTCCCTTCCAAAAGCCGCCTGGCGATTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                 <  2:3615135/137‑1 (MQ=255)
                                                                                                                             ttttATCGATATTCAAAACAAAACATGCACCTGGGGCTTATTAAAAAAGGAATGAAGCTCCCTACCAAAAGCCTCCTGGCGAGTCAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  2:181622/139‑1 (MQ=255)
                                                                                                                             cTTTTTCGCTATTTAAAAGAAAACATGAATCTGGGGCGTATTCAAAAAGGAATCACGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  2:3752461/140‑1 (MQ=255)
                                                                                                                             cTTTATCGCTATTTCAAATAAAACATGCATCCGGGGCTTTTTCAAAAAGTAATGAAGCTCCCTTCCAAACGACCCCTGGCGAGTCAGCTTCCCATCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  1:4566260/140‑1 (MQ=255)
                                                                                                                             cTTTATCGCTATTTAAAAGAAAACATGCATCCGGGGCTTATTAAAAAAGCAATGAAGCTCCCTTCCAAAACCCTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  1:4088224/140‑1 (MQ=255)
                                                                                                                             cTTTATCGCTATTACAAACAAAACAGGCACCCGGGGCTTATAAAAAAACCAATGACGCTCCCTTCCAAACCAGGCCGGGCTAGTCAGCTTTCCAACCCACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  1:1939282/140‑1 (MQ=255)
                                                                                                                             cTTTATCGATATTTAAAACAAAACATGCAACAGGGGCTTATTCAAAAAGGAATGACGCTCCCTTCCAAACGACTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  2:472358/140‑1 (MQ=255)
                                                                                                                             cTTTACCGATATTTAAAAGAAAACATGCATCCGGGGCGTTTTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTACCATCCGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  2:2858797/140‑1 (MQ=255)
                                                                                                                             atttAACGCTATTTCAAATAAAACATCCATCCGGGGCTTATAAAAAAAGGAATGAAGCTCCCTTCAACACCACTCCTGCCCAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa               <  1:3346727/139‑1 (MQ=255)
                                                                                                                                tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg            >  1:1727447/1‑140 (MQ=255)
                                                                                                                                  tCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                             >  2:5182020/1‑89 (MQ=255)
                                                                                                                                  ccgatATTTCAAACAAAACAGGCAACCGGGGCGTATACAAAAAGTAATCACGCTCCCTTCCAAACGACCCCTGGCGTGTCCGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt          <  1:5321662/136‑1 (MQ=255)
                                                                                                                                       aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                           <  1:1972036/86‑1 (MQ=255)
                                                                                                                                       aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                           >  2:1972036/1‑86 (MQ=255)
                                                                                                                                       aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc     >  2:4405358/1‑140 (MQ=255)
                                                                                                                                       aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTACCTTACAAAAGACTCCTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGCGCTATATc     >  2:3863970/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AACTGGAATTGGCCTTTTATAAACAGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTG  >  NZ_CP034943/548918‑549195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: