Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,119 T→G 8.8% L44R (CTG→CGG)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1190TG 8.8% 877.9 / 15.8 357L44R (CTG→CGG) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (181/129);  new base G (2/45);  total (183/174)
Fisher's exact test for biased strand distribution p-value = 1.71e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

ACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAAT  >  NZ_CP034943/548980‑549257
                                                                                                                                           |                                                                                                                                          
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                           >  2:104996/1‑140 (MQ=255)
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                           >  2:4309229/1‑140 (MQ=255)
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                           >  1:1749098/1‑140 (MQ=255)
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                           >  1:4569110/1‑140 (MQ=255)
  aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                                          >  1:5092918/1‑140 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                        >  1:5120364/1‑140 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                        >  2:5363364/1‑140 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                        >  2:2507069/1‑140 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                        >  2:4566260/1‑140 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                        >  2:4804661/1‑140 (MQ=255)
       aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                                     <  2:3163569/140‑1 (MQ=255)
       aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                                     <  2:5626016/140‑1 (MQ=255)
       aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                                     <  1:3439316/140‑1 (MQ=255)
       aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                                     <  2:1500412/140‑1 (MQ=255)
         aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                                   >  2:3924160/1‑140 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                                     >  1:1592783/1‑133 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                              >  1:4966566/1‑140 (MQ=255)
               atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTTGCGAGTCAGCTTTc                                                                                                                             >  1:4195160/1‑140 (MQ=255)
               atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                             >  2:3346727/1‑140 (MQ=255)
                 cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCATCTTTCCa                                                                                                                           >  1:5494504/1‑140 (MQ=255)
                 cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                           >  1:181622/1‑140 (MQ=255)
                  aCGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAt                                                                                                                          >  1:1361127/1‑140 (MQ=255)
                  aCGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAt                                                                                                                          >  1:4830419/1‑140 (MQ=255)
                   cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                         <  2:754816/140‑1 (MQ=255)
                   cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGAATTCAAAAAGGACTGAAGCTCCCTTCCAAAAGACTCCTGCCGAGTCAGCTTTCCATc                                                                                                                         >  1:2414529/1‑140 (MQ=255)
                      cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                                      >  2:2652852/1‑140 (MQ=255)
                         ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCaa                                                                                                                   >  1:4607894/1‑140 (MQ=255)
                           ttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGGGTATTAAAAAAGGAATGAAGCTCCCTTCCAAACCACTCCTGGCTTGTCAGCTTTCCATCCGTCAAAc                                                                                                                 <  2:68668/140‑1 (MQ=255)
                                tAGAAAGCTGGATAGCCCGCTTTATCCGCTGCTTTAGCGCTATTTCAAAGAAAAACGGCATCAGGGGCGTATTAAAAAAGGAATCAAGCCCCCTACCAAACGACTCCTGGCGAGTCAGCTTTCCATAAGGCAAACGACTg                                                                                                            <  2:5106588/140‑1 (MQ=255)
                                       gtgtatatTCCGATTTATCAGCAGCTTTATCGATATTTCAAACAAAACATCCATCAGGGGCGTATTCAAAAAGTAATGCAGCTCCCTTCCAAAACACTCCTGGCGATTCAGCTTTCAATCAGTCAAACGACTGTAGAACg                                                                                                     <  2:2155548/136‑1 (MQ=255)
                                       cgggATATTCCGCTTTATCAGCAGCTTTATCGATATTTCAAACAAAACAGGCATCAGGGGCGTATTAAAAAAGTAATGACGCTCCCTTCCAAACCACACCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                     <  2:3485629/138‑1 (MQ=255)
                                       cTGTATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAACAAAACATGCATCAGGGGCGTATTAAAAAAGTAATTAAGCGCCCTTCCAAACCACTCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACg                                                                                                     <  2:4963277/140‑1 (MQ=255)
                                       cTGTATATTCCGCTTTATCAGCAGATTTATCGATATTTCACATAAAACATGCATCGGGGGCGTATTCAAAAAGCAATCACGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACg                                                                                                     <  1:720507/140‑1 (MQ=255)
                                         ggATATTCCGCTTTATCAGCAGATTTAGCGCTATTACAAAGAAAACAGGCATCAGGGGCGTATAAAAAAAGGAATGAAGCTCCCTACCAAAACACTCCGGGCTAGTCAGCTTTCCAACAGTCACACGACTGTAGAACGTg                                                                                                   <  2:1565904/140‑1 (MQ=255)
                                          gATTTTCCGATTTATCAGCAGCTTTATCGATATTTAAAAGAAAACATGCATCCGGGGCGTATTAAAAAAGTAATGAACCTCCCTACCAAAAGACCCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                                  <  2:3041085/140‑1 (MQ=255)
                                               tCCGCGTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                             >  1:1022899/1‑140 (MQ=255)
                                                 cGCTTTCTCCGCAGGTTTATCGATATTTAAAATAAAACTTGCATCCGGGGGTTTTTAAAAAAGTAATGAAGCTCCCTTCCAACACACTCCTGGCGAGTCAGGTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGac                                                                                           <  2:1606505/140‑2 (MQ=255)
                                                 cGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGAGCCTATGaa                                                                                           >  2:3399495/1‑140 (MQ=255)
                                                 cGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGAATGTAGAACGTGCCTATGaa                                                                                           >  1:5439215/1‑140 (MQ=255)
                                                  gCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAAc                                                                                          >  1:5344661/1‑140 (MQ=255)
                                                   cTTTATACGCATATTTATCCATATTAAAACCAAAACAGGCATCCGGGGCGTATTAAAAAAGCAATGAAGGGCCCTTCCAAAAGACTCGTGGCGAGGCCGGTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACa                                                                                         <  2:3618367/140‑1 (MQ=255)
                                                    tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAATGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTCTGAACAg                                                                                        >  2:4395210/1‑140 (MQ=255)
                                                    tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                                                        >  1:271848/1‑140 (MQ=255)
                                                    tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                                                        >  1:2346394/1‑140 (MQ=255)
                                                    tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                                                        >  1:5201120/1‑140 (MQ=255)
                                                    tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                                                        >  2:4143112/1‑140 (MQ=255)
                                                        tCAGCAGCTTTATCGCTATTTCAAAGAAAACATCCATCAGGGGCTTATAAAAAAAGGAATGAAGCCCCCTTCCAAAAGACTCCTGGCGAGTCAGGTTTCCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                    <  2:732713/140‑1 (MQ=255)
                                                           gcagcTTTACCGCTATTTCAACGAAAACAGGAATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCACAAGACGCCTGGCGAGTCAGCTTGCCCTCAGTAAAACGACTGTAGAACGTGCCTATGAACAGCTtgctg                                                                                 <  2:4757031/140‑1 (MQ=255)
                                                           ccagcTTTATCGATATTTCAAAGAAAACATGAAACAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTACCAAAACACTCCTGGCGAGTAAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtgctg                                                                                 <  2:2778493/139‑1 (MQ=255)
                                                             agcTTTATCGCTATTACAAACAAAACCTGCATCCGGGGCGTATACAAAAAGGAATGAAGCGCCCTACAAAAACACCCCGGCCGAGTCAGCGTCCCAGCCGCCCCACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  1:3095914/140‑1 (MQ=255)
                                                             agcTTTATCGATATTTAAAACAAAACATGCATCAGGGGCTTATAAAAAAAGCAATCAAGCTCCCTACAAAAACACACCTGGCGAGTCAGCTTTCCAACAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  1:4309229/140‑1 (MQ=255)
                                                             agatttAACGCTATTTAAAACAAAACACCCACCAGGGGGGAAATAAAAAAGCATTGAAGCTCCATTCCAACCCACTCCTGCCGAGTCCGCTTCCCAACTGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  1:907496/137‑1 (MQ=255)
                                                             agagttATCGCTATTACAACAAAACCAGCCAACCGGGCCGTATAAAAACAGCAATCAAGCTCCCTTCCAACACACCCGGGCCTATTATCCTTTCCATCAGAAAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                               <  1:407828/136‑1 (MQ=255)
                                                              gatttATCGATATTAAAAACAACACAAGCAACCGGGGCGTATCAAAAAAGCACTGAAGCCCCCCTCCAAAACACGCCTGGCGAGTCAGCTGTCAAACACTCACACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                              <  1:2190534/138‑1 (MQ=255)
                                                               ctttcgcGCTATTTCAAACAAAACCTGCATCCGGGGCGTATTCAAAACGCAATGAAGCTCCCTTCAAAACGACTCCTGGCGAGTCAGCTGGCAATCAGTCACACGACTGTAGAACGTGCCTATGACCAGCTTGATGCTGa                                                                             <  2:1294/134‑1 (MQ=255)
                                                               cTTTTTCGCTATTTAAAAGAAAACATGAATCTGGGGCGTATTCAAAAAGGAATCACGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                             <  2:3752461/140‑1 (MQ=255)
                                                               cTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCCGTCAACCGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                             <  2:1740626/140‑1 (MQ=255)
                                                               atttATCGATTTTTCAAAGAAAACATGCATCCGGGGCGTATTCAAAATGGAATGAAGCTCCCTTCCAAACGACTCCTGGCGAGTCTGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                             <  2:5494504/139‑1 (MQ=255)
                                                                    tCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                           >  2:5182020/1‑89 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                         <  1:1972036/86‑1 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                         >  2:1972036/1‑86 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                   >  1:2619473/1‑140 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTACCTTACAAAAGACTCCTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGCGCTATATc                                                                   >  2:3863970/1‑140 (MQ=255)
                                                                            tCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTg                                                                >  2:2226617/1‑140 (MQ=255)
                                                                            tCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTg                                                                >  2:4177324/1‑140 (MQ=255)
                                                                               aataaaaCATGAATCTGGGGCGTATTAAAAATGTATTGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                             <  1:3924160/137‑1 (MQ=255)
                                                                               aacaaaaCATGCATCCGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCAAACAGACTCCGGGCGAGTCAGCTTTCCATCCGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                             <  1:1873614/137‑1 (MQ=255)
                                                                                      aaTGAATCAGGGGCTTTTAAAAAAAGTATTTAAGGTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTCCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaa                                                      <  2:900374/139‑1 (MQ=255)
                                                                                         gCATCAGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAATCGACTGTATAACGTGCCTATGAACAGCTTGCTGCTGAGGGCGATATCGTGAGCAAGCCatgaa                                                   >  2:3667282/1‑140 (MQ=255)
                                                                                         gCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaagaa                                                   >  2:4079656/1‑140 (MQ=255)
                                                                                           aTCCGGGGCGAATTCAAAAAGCAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCCGTAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGc                                                 <  2:3816782/140‑1 (MQ=255)
                                                                                             cAGGGGCGTATTAAAAAAGGAATGACGCACCCTCCCAAAAGACTCGTGGCGAGTCCGCTTTCCATCCTTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCgg                                               <  1:788235/140‑1 (MQ=255)
                                                                                                gggCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTAGATCGTGAGCAAGCCAAGAAGCGGATg                                            >  1:3033100/1‑140 (MQ=255)
                                                                                                   cttATTAAAAAAGGAATTAAGCTCCCTACCAAACCACGCCTGGCGAGTAAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtt                                         <  1:104996/138‑1 (MQ=255)
                                                                                                   cGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtt                                         >  2:4776217/1‑140 (MQ=255)
                                                                                                   cGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGAGGGtt                                         >  1:5545954/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGTAATTAAGCACCCTTCCAAAAGACTCCTGGCGAGACAGCTTACCATCAGTCAAACTACTGTAGAACGTCCCTATCAACACATTGCTGCGGAGGGCTATAGCGAGAGCAAGCCAACAAGCGGATGGTTTg                                       >  1:5746632/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGCCTGTAGAACGTGCCTATGAACAGCTTGCTGCTGCGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:755491/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:3748593/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:1725104/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  2:4645332/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:4025491/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGATGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                       >  1:3834236/1‑140 (MQ=255)
                                                                                                          aaaaaGGATTGACGCTCCCTACCAAAACACTCCTGGCGAGTCAGCTTTCCATCTGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                                  <  2:5268613/140‑1 (MQ=255)
                                                                                                          aaaaaGGAATTACGCTCCCTTCCAAACGCCTCCTGGCGAGTCAGCTTTCCCTCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                                  <  2:2446559/140‑1 (MQ=255)
                                                                                                            aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                >  2:5417618/1‑140 (MQ=255)
                                                                                                            aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                >  2:4440139/1‑140 (MQ=255)
                                                                                                            aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                >  2:1002433/1‑140 (MQ=255)
                                                                                                             aaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGGTTGCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTAt                               <  2:196385/140‑1 (MQ=255)
                                                                                                              aGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATc                              <  2:298666/140‑1 (MQ=255)
                                                                                                               ggAAGGCAGCTCACTTCAAAACGACGCGGGGCGAGACAGCTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCa                             <  1:3583859/140‑1 (MQ=255)
                                                                                                                           ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                 >  1:1148308/1‑140 (MQ=255)
                                                                                                                               ccAAAAGACTGCTGGTGATTAAGTTTTCCATCCTTCAATCGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg             <  2:5746632/140‑1 (MQ=255)
                                                                                                                                 aataGACTCGGGGCTAGTCAGCTTTCCATCAGTAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa           <  2:2414529/137‑1 (MQ=255)
                                                                                                                                 aaaaGACTCCTGGGGAGTCAGCTTGCCAGCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa           <  2:507427/140‑1 (MQ=255)
                                                                                                                                 aaaaGACTCCGGGCGATTCAGCTGTCCATCATTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa           <  2:1781317/140‑1 (MQ=255)
                                                                                                                                 aaaaGACTCCGGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa           <  1:2873484/140‑1 (MQ=255)
                                                                                                                                     gACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGAGGGTTGGCTGATTATCATGATTCTGATTTTGCGTATCGt       >  2:1216479/1‑140 (MQ=255)
                                                                                                                                      ccTCCTGGCGAGTCAGCTTACCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTa      <  1:2132520/139‑1 (MQ=255)
                                                                                                                                      aCTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTa      <  1:4782658/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAAT  >  NZ_CP034943/548980‑549257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: