BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	595,203	C→A	13.0%	T30K (ACG→AAG)	EO946_RS03130 →	MMPL family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	595,203	0	C	A	13.0%	708.1 / 26.1	300	T30K (ACG→AAG)	EO946_RS03130	MMPL family transporter
Reads supporting (aligned to +/- strand): ref base C (69/180); new base A (49/2); total (118/182)
Fisher's exact test for biased strand distribution p-value = 4.93e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

AGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTGAAGAA  >  NZ_CP034943/595064‑595341

      atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTTTTTTTTTCGAAATTAACGGAGCt                                                                                                                                      >  1:2445715/1‑140 (MQ=255)

      atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCt                                                                                                                                      >  2:3645931/1‑140 (MQ=255)

      atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTAGTTTGTTTTATACGAAATTAAAGGAGCt                                                                                                                                      >  1:3675728/1‑140 (MQ=255)

       ttCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTGTGTTTTGTACGAACTTAACTGATCtt                                                                                                                                     >  1:3186519/1‑139 (MQ=255)

       ttCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTGTCCGAACTTAAAGGAGCTg                                                                                                                                     >  1:4315087/1‑140 (MQ=255)

         cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                                                   <  2:3929406/140‑1 (MQ=255)

            cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTATACGAACTTAAAGGAGCTGTCCaa                                                                                                                                >  2:3177314/1‑140 (MQ=255)

            cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTTTTTTGTCCGAACGTAAAGGCTCTGGGCaa                                                                                                                                >  2:2405511/1‑140 (MQ=255)

            cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATACGAACTTAAACGAGCTGGCCaa                                                                                                                                >  2:2558029/1‑140 (MQ=255)

            cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTGTGCGAAATTAACGGCGCTGGCCaa                                                                                                                                >  1:3392120/1‑140 (MQ=255)

               tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACATAACGGAGATGTCCAAtaa                                                                                                                             >  2:4632397/1‑138 (MQ=255)

               tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTACGAACTTTACGGAGCTGGCCAAACa                                                                                                                             >  1:5492882/1‑140 (MQ=255)

               tCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGGTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCCATCa                                                                                                                             >  2:1359700/1‑140 (MQ=255)

                 tAAACAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGCGCAATTGCAGCGGTTATAGTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAATCaaa                                                                                                                           <  1:5335557/140‑1 (MQ=255)

                 tAAACAGCAAAGTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACGTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                                           <  1:1969175/140‑1 (MQ=255)

                 tAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                                           <  1:3496826/140‑1 (MQ=255)

                 tAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                                           <  2:1599076/140‑1 (MQ=255)

                 tAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTGTCCGAAAGTTAAGGTGCTGGCCACTCaaa                                                                                                                           >  1:5037184/1‑140 (MQ=255)

                   aaaaGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACATAAAGGTGCTGGCCAATCAAAAg                                                                                                                         >  2:1172205/1‑140 (MQ=255)

                      accAACTTTAAAGGAGGAGATTCTGCAGAATGAGAGCAAACATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                      <  1:4705619/138‑1 (MQ=255)

                      aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                      <  1:4467228/140‑1 (MQ=255)

                       gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                     <  1:4207021/140‑1 (MQ=255)

                       gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                     <  1:5173606/140‑1 (MQ=255)

                       gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                     <  1:2457304/140‑1 (MQ=255)

                       gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGATTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                     <  1:882778/140‑1 (MQ=255)

                       ccAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                     <  1:3976305/139‑1 (MQ=255)

                            tttAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGcc                                                                                                                <  1:2157226/140‑1 (MQ=255)

                            actacaggagcAGCTTCTGCAGCAGGAGAGCACTCAACAAATTCCAGCGCGCAAGTGCCGCGCGTATAGTAGCACGGACCGTCGTTTTGAGCTTGTTTTCTCCGAACTTAACGGAGCTGGCCACGCAAAAGGGGCAGGcc                                                                                                                <  1:3638176/135‑1 (MQ=255)

                                 aggaggAGATTCTGCAGAAGGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCt                                                                                                           <  1:920103/140‑1 (MQ=255)

                                 aggaggAGAGTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCt                                                                                                           <  1:268682/140‑1 (MQ=255)

                                      gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCATg                                                                                                      >  2:3279310/1‑140 (MQ=255)

                                      gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATCCGAACATAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTg                                                                                                      >  2:886528/1‑140 (MQ=255)

                                      gagaTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCCGGCCCAGCTTCCTg                                                                                                      >  2:1064140/1‑140 (MQ=255)

                                         aTTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGTGTTTGTTTTCTCCGGACTTAACGGAGCTGGCGCATCAAAAGGGGCAGGCCCACCTTCCTGCAg                                                                                                   >  2:1792251/1‑140 (MQ=255)

                                            cTGCAGAATGAGCGCAATCAGCAAATTCAAGGGGGCAATTGCCGCGGTTATATTAGCACTGACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                                <  2:563781/140‑1 (MQ=255)

                                            cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                                <  2:639329/140‑1 (MQ=255)

                                            cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                                <  1:5717273/140‑1 (MQ=255)

                                            cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                                <  1:2290442/140‑1 (MQ=255)

                                              gCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGGGTTTTTTTTTTGCGAAATTTACGGAGCTGGGCCATCAAAAGGGGGAGGCCCCGCTTCCTGCCGATTCt                                                                                              >  1:573974/1‑140 (MQ=255)

                                                    tGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTGTACGAAAGTAAAGGAGCTTGCCAATCAAAATGGGCAGGCCCACCTTCCTGCAGATGCTGtttag                                                                                        >  1:3288552/1‑138 (MQ=255)

                                                      agagCAATCTTCAAATTTTATTGGGCAAGTGCAGGGTTTATATTAGCACTTTCGGTCGTTTTGAGTTTGTTTTCTACTATCTTAACGGAGCTGGCGAATGAAAAGGGGCTTGCCCAGCTTCCTGCATATGCTGTTTCAGa                                                                                      <  2:3522216/140‑1 (MQ=255)

                                                       gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATGCGAACTTAACGGAGCTGGGCAATCAAAAGGGGCAGGCCCAGCATCCAGCACATGCTGTTTCAGaa                                                                                     >  2:251347/1‑140 (MQ=255)

                                                       gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTAGTTTGTTTTCTCCGAAATTAACGGAGCTGGCCAAACAAAAGGGGCACGCCCAGCTAACTGCAGATGCTGTTTCATaa                                                                                     >  2:3113048/1‑140 (MQ=255)

                                                       gagCAATCATCAAATTCAAGTGGGCAATCGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGCACTTAACCGAGCTGGCCAATCAAAAGGGGCAGGCCCACCATCCAGCAGATGCTGGTTCAGaa                                                                                     >  2:2677784/1‑140 (MQ=255)

                                                        agCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGaaa                                                                                    <  1:1653539/140‑1 (MQ=255)

                                                        agCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATAGTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGaaa                                                                                    <  2:2733694/140‑1 (MQ=255)

                                                            atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTTTTTTGTCCGAAATTAAACGAGCTGGCCAAAAAAAAGGGGCACGCCCCCCTTACTGCCGATTCTGTTTGAGaaaaatc                                                                                >  1:3321132/1‑136 (MQ=255)

                                                            atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATCCGAACTTAACGGAGCGTGCCAATCAAAAGGGGCAGGCCCAGCATACTGCCGATGCTTTTTCAGAAACAGc                                                                                >  2:3551170/1‑140 (MQ=255)

                                                            atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTGGTGTTTGTTTTGTCCGAACTTAACGGAGCTGGCCAATCAAAACGGGCCGGCCCACGTTCCTGCAGCTGCTGTTTCGGAAacaga                                                                                >  2:5719522/1‑139 (MQ=255)

                                                            atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTCTCCGAAATTAACGGAGCTGGCCAAAAAAAACGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTAAGAAAGAGc                                                                                >  2:1840856/1‑140 (MQ=255)

                                                            atcatcAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTATCCGAACTTAACGGAGCTGGCCCATCAAAAGGGGCAGGCCCACCTTCCTGCAGATGCTGTTTAAGAAACAGc                                                                                >  2:2344880/1‑140 (MQ=255)

                                                                  aaaTTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGc                                                                          <  1:1172205/140‑1 (MQ=255)

                                                                       cAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTTTTTTTTTTATCCGAACTTAAAGGAGCTGGCCAAACAAAAAGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTAAGTAATAGCAAAAGCCAttt                                                                     >  1:255194/1‑140 (MQ=255)

                                                                         aGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTCTCCGAAATTAACGGAGCGGGCCAAACAAAAGGGGCAGGCCCAGCTTCCTGCCGATGCTGGTTAAGAAACAGCAAATGCCATTTTa                                                                   >  1:5688220/1‑140 (MQ=255)

                                                                            gggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTaaaa                                                                <  1:3587414/140‑1 (MQ=255)

                                                                             ggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTTTTTTCTCCGAACTTAACCGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCATCCTGCAGATGCTGGTTAAGAAATAGCAAATTCCATTTTaaaaa                                                               >  2:3593670/1‑139 (MQ=255)

                                                                                aaTTGGAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCGGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAg                                                            <  1:3769049/140‑1 (MQ=255)

                                                                                       gCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGgaga                                                     <  2:5529524/140‑1 (MQ=255)

                                                                                       gCGGTTATATTAGCACTTACCGTCGTGGTGTGTTTTTTTTCTCCGAACTTAACGGGGGTGGCCCATGAAAATGGGCAGGCCCCCCTTCCTGCAGCTGCTTTTTGAGAAACCGCAAATGCCCTTTTAAAACAAGCCGgaga                                                     >  1:5661647/1‑140 (MQ=255)

                                                                                        ctgTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGagaa                                                    <  2:5063380/138‑1 (MQ=255)

                                                                                          gTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAAATTAACGGAGCTGGCCAATCAAAAGGGGCCGGCCCAGCATCCTGCAGATGCTGTTTAAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGagaaga                                                  >  2:346267/1‑140 (MQ=255)

                                                                                                 tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                                           <  2:1468317/140‑1 (MQ=255)

                                                                                                 tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                                           <  2:2006331/140‑1 (MQ=255)

                                                                                                 tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                                           <  2:2916858/140‑1 (MQ=255)

                                                                                                 gaGCACTTAGCGTCGTGTGGAGGTTGTTTTCGCCGAACTTAACGGAGCTGGCCAATCACAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGGTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                                           <  2:4969388/139‑1 (MQ=255)

                                                                                                    cACTTACCGTCGTTTTGTGTTTTTTTTATCCGAAATTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCGTCCTGCAGAAGCTGTTTGAGAAAGAGCAAAATCCATATTAAAAAAACCCGGAGAAAACaaaaaatac                                        >  2:4800570/1‑136 (MQ=255)

                                                                                                    cACTTACCGTCGTTTTGAGTTTTTTTTGTCCGAAATTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCCTTTTAAAAAAAGCGGGAGAAGACAATAAttag                                        >  2:3806186/1‑138 (MQ=255)

                                                                                                     aCTTACCGTCGTTTTGTGTTTTTTTTATACGAACTTAAACGAGCTGGCCCATCAAAAGGGGCAGGCCCACCTTCCTGCAGATGCTGGTTAAGCAAGAGCAAAAGGCATTTTAAAAAAAGCGGGAGAAAACAATAAATCGa                                       >  2:5475305/1‑140 (MQ=255)

                                                                                                     aCTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGa                                       <  1:2405511/140‑1 (MQ=255)

                                                                                                     aCTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGa                                       <  1:1008367/140‑1 (MQ=255)

                                                                                                      ctgagcgtcgtTTTGAGTTTGTTGTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAGGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:2971135/135‑1 (MQ=255)

                                                                                                      cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  1:557079/140‑1 (MQ=255)

                                                                                                      cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                      <  2:5766250/140‑1 (MQ=255)

                                                                                                        tACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTAGAt                                      >  2:3396393/1‑138 (MQ=255)

                                                                                                             tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  2:787139/140‑1 (MQ=255)

                                                                                                             tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  2:5328422/140‑1 (MQ=255)

                                                                                                             tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  1:1219251/140‑1 (MQ=255)

                                                                                                             tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  2:2201149/140‑1 (MQ=255)

                                                                                                             tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  2:2451412/140‑1 (MQ=255)

                                                                                                             tcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATc                               <  1:3021376/140‑1 (MQ=255)

                                                                                                                tgttGTGTTTTTTTTCTCCGCACTTAAACGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGACAGAGCCAAAGCCATTTTTAAACAATCCGGAGACTACAAAAATTCGATAAGTATCGTc                            >  2:3319583/3‑140 (MQ=255)

                                                                                                                tgttGAGTTTTTTTTATCCGCAAATAACCGATCTGTCCCATCAAAAGGGGCAGGCCCAGCATCCTGCAGATTCTGTTTCAGAAAGAGCAAATGACAATTTAAAACAAACGGGATAAGAAAATAATTCAATCAGTATCGta                            >  2:940432/3‑139 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  2:4242055/140‑1 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:2908411/140‑1 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:1420251/140‑1 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:2805618/140‑1 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:1766107/140‑1 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  1:1321352/140‑1 (MQ=255)

                                                                                                                 tttGAGTTTGTTTTCTCCGAACTGAACGGATCTGGCCAATCAAAAGGGGCATGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                           <  2:434634/140‑1 (MQ=255)

                                                                                                                  ttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGTCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCtt                          <  1:1381483/140‑1 (MQ=255)

                                                                                                                  ttGAGTTGGGTTTCACCGAACTTATCTGAGCTTGAGAATCAAAGTGGTCAGGCCGAGCTTCCTTCCGATGCTTTTTCATATATATCATATTCCATTTTAAAACCACCGGGAGAAGGCAATAATTCGATCAGTATCGTCtt                          <  1:4851258/140‑1 (MQ=255)

                                                                                                                      gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa                      <  1:547439/140‑1 (MQ=255)

                                                                                                                      gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa                      <  1:699770/140‑1 (MQ=255)

                                                                                                                             ttCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATc               <  2:4100945/140‑1 (MQ=255)

                                                                                                                                  cGAACTTAACGGAGCTGGCCAAGCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCt          <  2:4830372/140‑1 (MQ=255)

                                                                                                                                     aCTTAACGGAGCTGGCCAAGCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTg       <  1:1266753/140‑1 (MQ=255)

                                                                                                                                         aaCGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaaga   <  1:2051166/140‑1 (MQ=255)

                                                                                                                                         aaCGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaaga   >  2:4011515/1‑140 (MQ=255)

                                                                                                                                         aaCGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaaga   >  2:1271653/1‑140 (MQ=255)

                                                                                                                                         aaCGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaaga   <  2:1213688/140‑1 (MQ=255)

AGAGATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTGAAGAA  >  NZ_CP034943/595064‑595341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`