Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,359 A→C 13.6% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,3590AC13.6% 528.6 / 13.2 274T68P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (92/123);  new base C (8/41);  total (103/171)
Fisher's exact test for biased strand distribution p-value = 5.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498
                                                                                                                                          |                                                                                                                                           
     gggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAg                                                                                                                                       <  2:4553925/140‑1 (MQ=255)
     gggACCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAGCCGGACCAACCGGAg                                                                                                                                       >  2:4638162/1‑140 (MQ=255)
         gCTACTGGGGAAGCTGGACCAGCCGGACCATACGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGAGCAGCACGAGCGACGCGAGCGACAGGAGCGACCGGGGGAGCCGGACCAGCGGGCGCCTCGGGAGCGAc                                                                                                                                   >  2:4911876/1‑140 (MQ=14)
         gCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCAGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTCCCGGGGCAGCCGGAGCAGCCGGCGCAGCCGGAGCGAc                                                                                                                                   >  2:430878/1‑140 (MQ=17)
         gCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACGGGAGCGAc                                                                                                                                   >  2:5232927/1‑140 (MQ=255)
         gCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAACCGGACCAGCCGGACCAACCGGAGCGAc                                                                                                                                   >  2:3236521/1‑140 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  >  1:4460342/1‑140 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:878722/140‑1 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGCACCATCCGTAGCGACGGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCGGACCAACCGCAGCGACg                                                                                                                                  >  1:5647716/1‑140 (MQ=21)
            agtGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:3920332/138‑1 (MQ=255)
               ggggAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACAGGGGAAGCCGGACAAGCGGGACCACCCGGAGCGCCCGGAGc                                                                                                                             >  2:2508401/1‑140 (MQ=18)
                  gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  1:43592/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:3119543/140‑1 (MQ=255)
                      cTGGCCCAGCCGGACCAGCCGGAGCGACTGGAGCCACGGGAGCGACCGGGGAAGCTGGACCTGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGa                                                                                                                      <  2:3187614/140‑1 (MQ=21)
                      cTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGa                                                                                                                      <  2:531269/140‑1 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAg                                                                                                                     >  1:653636/1‑140 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAg                                                                                                                     <  1:1532049/140‑1 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGCGAAGCTGGACCAGCCGGAGCCACGGGAGAGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAg                                                                                                                     >  1:613858/1‑140 (MQ=255)
                       tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGTCCAGCCGGACCCACCGGCGCGACGGGGGCCACGGGAg                                                                                                                     >  1:1734379/1‑140 (MQ=18)
                          accagccggaccagccggAGCGACGGGAGCCACTGGTGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:4216132/140‑1 (MQ=255)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:2249718/140‑1 (MQ=255)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCCGCCGGACCAACCGGAGCGAGGGGCGCGAGGGGAGCtc                                                                                                                  >  2:4084137/1‑139 (MQ=18)
                          accagccggaccacccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCAGGAGCGACGGGAGCGACAGGAGCTACCGGAGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  >  1:4610875/1‑140 (MQ=255)
                          accaccaggcccacccggAGCCCCCGGAGCCACCGGAGCTACCGGAGAACCTGGACCAGCCGGAGCCCCGGGAGCCACCGGAGCGACCGGCGATCCCGGTCCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                                                                  <  2:5564043/140‑1 (MQ=9)
                               ccggaccagccggaGCGACGGGAGCCACTGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  2:4381106/140‑1 (MQ=255)
                               ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:1008229/140‑1 (MQ=255)
                                    ccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGCCGGGAGCCTCGGGAGCTACCGGGGAagc                                                                                                        >  1:1414971/1‑140 (MQ=255)
                                        ccggaGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    <  2:1358975/140‑1 (MQ=255)
                                        ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    >  1:744470/1‑140 (MQ=255)
                                        ccggaGCGACGGGAGCCACGGGAGCTACCGGGCAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    <  2:3496488/140‑1 (MQ=255)
                                            aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGCGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  2:3967892/140‑1 (MQ=255)
                                                aCGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg                                                                                            >  1:3511770/1‑140 (MQ=255)
                                                 tgggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCCACTGGAGCTACGGGGGAAGCGGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  1:958964/139‑1 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGCGCCACGGGAGCTACCGGGGAagccggaccagccggc                                                                                           >  2:4804359/1‑139 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  1:2295232/140‑1 (MQ=255)
                                                        cACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGCGCAGCCGGACCAGCCGGACCGAcgg                                                                                    >  2:2955034/1‑138 (MQ=17)
                                                        cACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTCCAACCGGAGCGACGGGGGAGACGGGGGCTACCGGGGAAGCCGGAGCGGCCGGACCAACCg                                                                                    >  2:4194359/1‑140 (MQ=18)
                                                         aCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                   <  1:496213/140‑1 (MQ=255)
                                                              agatACCGGGGAAGCTGGACCAGCCGGAGCCTCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcga                                                                              <  2:684172/137‑1 (MQ=255)
                                                              aGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcga                                                                              <  2:589611/140‑1 (MQ=255)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  1:2830007/140‑1 (MQ=255)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  1:78725/140‑1 (MQ=255)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            >  2:5460177/1‑140 (MQ=255)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                 <  2:29854/135‑1 (MQ=255)
                                                                   cgggcgAACCTGGACCCGCCGGACCCACGGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagga                                                                         <  2:4720747/135‑1 (MQ=17)
                                                                    cGGGGACGCGGGCCCAGCCGGCGCGACGGCACCCACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                        <  1:2271113/140‑1 (MQ=14)
                                                                    cGGGGAAGCTGGACCCGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCACCCGGACCACCCGGAGGGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                        <  1:990049/140‑1 (MQ=17)
                                                                       tgAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                     <  2:3688581/139‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:1492944/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGTGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:5022938/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:3269699/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:3793287/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:922893/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:4272917/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGACGGGAGCCACAGGAGCGACCGGGGAACCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:5399598/140‑1 (MQ=255)
                                                                        gAAGCCGGACCAGGCGGTGCGTCGGGAGCCCCCGTCGCTACCGGGGATGCCGGACCAGCCGGACCCCCAGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:2353389/140‑1 (MQ=14)
                                                                        gAAGATGGACCAGCCGGAGCGCCGGGAGCGACAGGACCTCCCGGGGAAGCCGGACCAGCCGGACCCCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:2305886/140‑1 (MQ=17)
                                                                            cTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACGGGAGCCCCCGGAGCTACCGGTGCGACCGGAGCAGCCGCAGCTACCGGAgcgacagctgcgagagga                                                                >  1:209457/1‑140 (MQ=14)
                                                                             tGGACCAGCGGGAGCGCCGGGAGCGACTGGAGCTACCGGGGAATCCGGACCTGCCGGACCACCCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  1:2424872/140‑1 (MQ=17)
                                                                             gggACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:4827996/139‑1 (MQ=255)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAGGGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCTAGCGGAGCGACGGGAGCGACCGGAGCTagggg                                                        >  2:2807227/1‑136 (MQ=14)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCAACGGGAGCTACCGGAGAAGCCGGAGCGGCCGGAGCTACCGGCGCGACCGGAGCGGCCGGAGCTACCgg                                                        >  2:17022/1‑140 (MQ=14)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCACCGGAGCTACCGGGGCCGCCGGACCGGCCGGACCACGCGGAGCGACAGGCGCGACCGGAGCTACCgg                                                        >  2:655570/1‑140 (MQ=14)
                                                                                     ccGGAGCGCCGGGCGCGAGAGGAGCGACCGGGGCAGCCGGACCAGCCGGACCAACCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:2113005/140‑1 (MQ=17)
                                                                                          gCGACGGGAGCGACAGGAGAATCCGGGGCAGCCGGACCCGCCGGAGCAACCGGAGCTGCCGGAGCCCCCGGAGCACCCGGGGGCGCCGGCGCAGCCGGCCCTACCGGAGCGACCGGGGGGGCCGGAGCTGCCggggcggc                                                  >  2:4028264/1‑136 (MQ=255)
                                                                                              caggAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                              <  1:2699228/138‑1 (MQ=14)
                                                                                               gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:4260831/140‑1 (MQ=255)
                                                                                                    cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGTCGGACCAGcc                                        <  1:4296605/140‑1 (MQ=255)
                                                                                                      actggAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                      <  1:3236521/137‑1 (MQ=255)
                                                                                                      aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                      <  1:106296/140‑1 (MQ=255)
                                                                                                         ggAGCTACCGGGGATGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagc                                   <  1:4042430/140‑1 (MQ=255)
                                                                                                         ggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagc                                   <  1:5498027/140‑1 (MQ=255)
                                                                                                           aGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcga                                 <  2:46873/140‑1 (MQ=255)
                                                                                                           aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAGCCggagcga                                 >  2:1766800/1‑140 (MQ=255)
                                                                                                           aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCCGCCggagcga                                 >  2:2642495/1‑140 (MQ=14)
                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                               <  1:3628206/140‑1 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             >  2:5754841/1‑140 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             <  2:5142827/140‑1 (MQ=255)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggag                           >  1:4808577/1‑140 (MQ=255)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggag                           >  1:542192/1‑140 (MQ=255)
                                                                                                                   gggAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcg                         <  1:3052380/140‑1 (MQ=255)
                                                                                                                    cgctcccggtcaagccggacgaGCCGGAGGTGCCGTAGCGACCGGAGCTACAGGCGCAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:1037447/135‑1 (MQ=11)
                                                                                                                     gAAGCCGCACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                       <  2:952563/140‑1 (MQ=255)
                                                                                                                       agccggaccagccggaccAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacag                     >  2:3622896/1‑140 (MQ=255)
                                                                                                                        gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCGACCGGAGCGACCGGAGCGACAGGAGCTACGGGGGACGCCGGACCAGCCcgagcgacaggggcgacagg                    >  1:1703716/1‑140 (MQ=14)
                                                                                                                        gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGCGGCCGGGGCATCCGGACCGCCCggagcgacaggggcgacggg                    >  2:2140524/1‑140 (MQ=14)
                                                                                                                          cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacaggcg                  >  1:4703173/1‑138 (MQ=255)
                                                                                                                          cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacaggag                  >  2:3423153/1‑140 (MQ=255)
                                                                                                                          cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                  >  1:1170519/1‑140 (MQ=255)
                                                                                                                          cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCggagcgccaggagcgacaggag                  >  2:3434429/1‑140 (MQ=255)
                                                                                                                          cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGACCTACCCGGGCACCCGGACCCGCCGGACCACCCGGAGCCACCGGAGCGGCAGGAGCGACCGGGGCAGCCGGCCCAGCCggcgcgaccggcgcgcgcgggg                  >  2:263583/1‑133 (MQ=17)
                                                                                                                             cccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga               <  1:3580770/139‑1 (MQ=255)
                                                                                                                              ccccccggaccaCCCGGCGCGACGGGACCAACCGGAGCGACGGGCGAACCAGGCCCACCCGGCCAACCCGGAGCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagccacgggagccac              <  1:3305769/136‑1 (MQ=9)
                                                                                                                              ccagccggaccaACCGGAGCCACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  1:408574/140‑1 (MQ=255)
                                                                                                                               cagccggaccaCCCGGAGCGACGGGAGCCCCGGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgaca             <  2:3648307/140‑1 (MQ=255)
                                                                                                                               cagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  2:1960230/48‑1 (MQ=38)
                                                                                                                               cagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         >  1:1960230/1‑48 (MQ=38)
                                                                                                                                 gccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCGACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  1:91195/140‑1 (MQ=255)
                                                                                                                                 gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           >  2:1129840/1‑140 (MQ=255)
                                                                                                                                          aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  >  2:3874885/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: