BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,469	A→C	5.8%	T104T (ACA→ACC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,469	0	A	C	5.8%	450.8 / 3.4	213	T104T (ACA→ACC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (72/118); new base C (5/17); total (77/136)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

CTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGA  >  NZ_CP034943/607330‑607607

cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                                                                                                                                            <  1:251874/140‑1 (MQ=255)

  accggagcgacgggagccacaggggctactggggacggcggaccagccggagcgacaggagctaccggggacgccggaccaaccggagcgacgggagccacaggggcgcctgggcacgtCGGACCCGCCggggcgccagg                                                                                                                                          >  2:4210425‑M2/120‑140 (MQ=255)

  accggagcgacgggagccacaggggctactgggcacgtcggaccagccggagcgacaggagctacaggggaagccggaccaaccggagcgaccggagccacaggggcgactggcgacggCGGCGCAGCCggagcgccagg                                                                                                                                          >  2:1914007‑M2/120‑140 (MQ=255)

  aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                                                                          <  2:5142827/140‑1 (MQ=255)

   ccGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACGGGAGCCCCGGGAGCGACCGGGGACGCCGGACCAGCCGGAGCAACCGGAGCGACCGGAGCGACAGGAGCGACCGGGGAAGCCGGGGCGGCCggagcgaccgga                                                                                                                                         >  2:900490/1‑140 (MQ=14)

    cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggag                                                                                                                                        >  1:542192/1‑140 (MQ=255)

      gggAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcg                                                                                                                                      <  1:3052380/140‑1 (MQ=255)

         aaGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                                                                                   <  1:5491098/140‑1 (MQ=255)

           gccggaccagccggaccaACCGGAGCGACGGGCGCCACGGGCGCAACGGGGCATCGCGGACCATCACGCCCCAGCGGAGGGACAGGAGCGACCGGTGGTACCTCGCCGGCCGGGCCAGCCggggggaccggcgcggcagg                                                                                                                                 >  2:4252995/1‑140 (MQ=255)

            cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtCGGACCAGCcggagcgacaggagctaccggg                                                                                                                                >  2:2302069‑M2/110‑139 (MQ=255)

            cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggcgactggggacgtCGGACCAGCcggagcgccagcagctaccggg                                                                                                                                >  2:3478739‑M2/110‑139 (MQ=255)

             cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggcg                                                                                                                               >  2:1536350/1‑138 (MQ=255)

             cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                                                               >  1:1170519/1‑140 (MQ=255)

             cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                                                                               >  1:616087/1‑140 (MQ=255)

             cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGACCTACCCGGGCACCCGGACCCGCCGGACCACCCGGAGCCACCGGAGCGGCAGGAGCGACCGGGGCAGCCGGCCCAGCCggcgcgaccggcgcgcgcgggg                                                                                                                               >  2:263583/1‑133 (MQ=17)

                cccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                                                                            <  1:3580770/139‑1 (MQ=255)

                accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                                                                            >  1:1478448/1‑140 (MQ=255)

                 ccagccggaccaACCGGAGCCACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                                                                           <  1:408574/140‑1 (MQ=255)

                  cagccggaccaGCCGTAGCGCCGGGAGCCACGGGAGCTCCCGGGGAATCCGGACCAGCCGGACCAACCGGAGCGAAAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgaca                                                                                                                          <  2:3648281/140‑1 (MQ=14)

                    gccggacccCCCGGAGCGCCGGGAGCCCCGGGCGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                        <  2:5739289/140‑1 (MQ=17)

                    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                        >  2:1129840/1‑140 (MQ=255)

                    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                        <  2:3072952/140‑1 (MQ=255)

                    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                        <  1:3622896/140‑1 (MQ=255)

                    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                        <  1:4123909/140‑1 (MQ=255)

                     ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGTAAGGCGGTCCAGCCggagcgacaggagcgtctggagcccccgga                                                                                                                       >  1:1432498/1‑133 (MQ=17)

                     ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                                                                                                                       <  1:4636138/140‑1 (MQ=255)

                      cggacccCCCGGAGCCACGGGAGCCACGGGAGCTACCGGGGACCCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                                                                                                                      <  2:943359/140‑1 (MQ=17)

                         accaccCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                   <  1:22910/140‑1 (MQ=255)

                             aCCGGACCGCCCGGAGCCACGGGAGCAACCGGCGAACCCGGCCCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                               <  1:5591824/140‑1 (MQ=14)

                              ccGGTGGGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                              <  1:2401875/140‑1 (MQ=14)

                              ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGCAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                              <  2:5468353/140‑1 (MQ=255)

                              ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGCGCTCGCGGAGATACCGGGCCCGCCGCACCAGCCCGCGCGTCATGGGGGAGCGGTGCGACGGGAGCTACCggg                                                                                                              >  1:2010368/1‑140 (MQ=9)

                              ccGGAGCCCCGGGACCCCCGGGAGCTCCCGGGGAAGCCGGACCCGCAGCACCAACCGGAGCGACTGGTGCGACAGGAGCTACCGGGGAAGCCGGTCCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                              <  1:2657074/140‑1 (MQ=14)

                               cGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTCCCGGGGACGCCGGCCCAGCCGGGGCGACAGGAGCGCCAGGAGCGACCGGAGCGCCCgggg                                                                                                             >  1:3845216/1‑140 (MQ=14)

                                  agccccGGGAGCCACGGGATGAACCGGGCATCCCGGACCAGCCGTACCAAACGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                          <  2:3674060/135‑1 (MQ=14)

                                  aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                                                                          <  1:2565542/108‑1 (MQ=255)

                                       cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                                                                     >  2:2084470/1‑140 (MQ=255)

                                             ccccGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                               <  1:3734358/137‑1 (MQ=255)

                                             ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                               <  1:1129840/140‑1 (MQ=255)

                                                 gggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                                                                     >  2:1070360/1‑130 (MQ=255)

                                                    aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGa                                                                                        >  1:4435359/1‑140 (MQ=255)

                                                        actggggAAGCTGGACCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                    <  2:4292545/137‑1 (MQ=255)

                                                        aCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                    >  1:4263012/1‑140 (MQ=255)

                                                        aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                    >  2:3956870/1‑140 (MQ=255)

                                                           ggggAAGCCGGACCAGCCGGACAAACCGGATCGACATGAGCCACAGGAGCTCCGGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACAGGCGCTACCGGGGCAGCTGGAGCAGCGGGAGCGCCgggggg                                                                                 >  1:1196187/1‑137 (MQ=14)

                                                                 gccggaccagccggaccaCCCGGAGCGACTGGAGCGACAGGATCGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                           <  2:2860378/140‑1 (MQ=17)

                                                                 gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                           >  2:3345522/1‑140 (MQ=255)

                                                                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGAGCGACAGGAGCTACCGGGCAAGCAGGACCACCAGGAGCGACGGGAGCGACAGGAg                                                                         >  1:3691526/1‑140 (MQ=14)

                                                                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                         >  1:3494240/1‑140 (MQ=255)

                                                                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                         >  1:1917128/1‑140 (MQ=255)

                                                                      ccccgccggccccACCGGACCCACCGGAGGGACTGGCGCTCCGGGCGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACAGGAGCGACAGGAGCTa                                                                      <  2:136002/136‑1 (MQ=14)

                                                                           ccggaccaACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGCGCGACAGGAGCGTCAGGAGCAACCGGATAAGCTGGAGCAGCTGGAGAGACGGGAGCGAGAGGAGCTACAggg                                                                 >  1:4832383/1‑140 (MQ=17)

                                                                               accaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                                                                                 <  2:655817/72‑1 (MQ=255)

                                                                               accaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                                                                                 >  1:655817/1‑72 (MQ=255)

                                                                                ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGCGACCGGAGCGGCCGGGGAAGCTGGCGCAGCGGGAGCGCCCGGAGCGCCCGGAGCTACGGGGGAAGc                                                            >  2:1863386/1‑140 (MQ=17)

                                                                                    ccGGAGCGACAGGATCTACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCGACAGGAGAGACAGTACAAACCGGGGACGCTGGAGCAGCTGGCGCGACGGGAGCGACAGGAGCGACAGGGGACGCCGGa                                                        >  2:5387497/1‑140 (MQ=17)

                                                                                    ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAGAGGAGCTACAGGAGAGACAGGACCTACCGGTGACGCTGGAGCAACCGCAGCGACGGGAGCGACAGGAGCGACCGGGGAGGCCggg                                                        >  2:2189461/1‑139 (MQ=17)

                                                                                    ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                        >  2:4461020/1‑140 (MQ=255)

                                                                                     cGGCGCGCCCGGAGCGCCCGGAGCTACCGGGGAAGCCGGACCAGCCGCAGCGACCGGAGCGACAGGAGGGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  1:2729035/140‑1 (MQ=17)

                                                                                     cGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  2:4893128/140‑1 (MQ=255)

                                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  1:2084470/140‑1 (MQ=255)

                                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  2:1170519/140‑1 (MQ=255)

                                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  2:4808577/140‑1 (MQ=255)

                                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  1:3345522/140‑1 (MQ=255)

                                                                                     cGGAGCGACAGCCGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCGACAGTAGCGACTGGATCGACAGGTGCGCCCGTTGACGCTGGCCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                       <  1:3536371/140‑1 (MQ=17)

                                                                                         gccaccggagcgccaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                   <  2:594339/134‑1 (MQ=18)

                                                                                             caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACCGGAGCAACCGGGGAAGCGGGCCCAGCGGGAGCGACGGGCGCAACAGGAGCACCCGGGGCCGCCGGCCCGGCCggg                                               >  2:1630738/1‑140 (MQ=17)

                                                                                             caggagcgacaggagcTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGGGCGCCAGGCGCCACGGGCCACGCGGCGCCAGCTGGAGAGCCGGGCGCCAGAGGACCTACCGGGGACGCCGGAGCAGCgggg                                               >  2:3138595/1‑140 (MQ=12)

                                                                                                gcgccccaggagcTACCGGGGAAGCGGGACCAGCCGGAGGGACAGGCGGGACTGGAGCGTCAGGTGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGTCAGGAGCTACCGGGGAAGCCGGACCATCCGGGGcc                                            <  2:4610875/134‑1 (MQ=17)

                                                                                                     ccaggagcTCCCGGCGAAGCCGCACCAGCCGGAGCCACAGGAGCCACAGGAGCGACCGGTGCTACCGGCGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                       <  2:3726713/139‑1 (MQ=18)

                                                                                                     accggagcTACCGGCGAAGCCGGACCCGCCGGAGCCTCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                       <  1:5333737/137‑1 (MQ=18)

                                                                                                         gccctcccGGTGAACCCGGACCAGCCGGAGCCCCAGGAGCGACAGGAGCCCCAGGAGCAACCGGTGAACCAGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                   <  2:1435667/134‑1 (MQ=17)

                                                                                                          agcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                  <  2:4435359/140‑1 (MQ=255)

                                                                                                          agcTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGCGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                  <  1:1691821/140‑1 (MQ=255)

                                                                                                           gcTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                 <  1:686948/140‑1 (MQ=255)

                                                                                                           gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGATACCGGGGAAGCTGGACCACCTCGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGAGCAGCCGGGGCCCCGGGCGCcac                                 >  1:4991678/1‑140 (MQ=18)

                                                                                                           gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                 <  1:85794/140‑1 (MQ=255)

                                                                                                            cTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                <  1:1025026/140‑1 (MQ=255)

                                                                                                            cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                <  1:3956870/140‑1 (MQ=255)

                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                              <  1:4007721/140‑1 (MQ=255)

                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                              <  1:1766800/140‑1 (MQ=255)

                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCAACCGGCGACGCTGGACCAGCTGGCGCGACGGGAGCGACAGGAGCACCCGACGACTCGGGAACATCCGGATCAACGGGAGCCACAgg                              >  1:5177064/1‑140 (MQ=17)

                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                              <  1:5666855/140‑1 (MQ=255)

                                                                                                               ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCAGGACCAGCTGGAGCGACGGCAGCGACAGGAGCTAACGGCGAAGCAGGACCAGCCGGGGCGACGGGAGCCACAggg                             >  1:4502141/1‑140 (MQ=21)

                                                                                                                cgcgcacgCCGCACCCGCCGGCCCCCCAGGACCGACCGGAGCGCCCGGAGCGACCGGGGAAGCTGGACCAGCGGCAGCCACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                            <  2:195653/133‑1 (MQ=17)

                                                                                                                cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                              <  1:4923980/58‑1 (MQ=255)

                                                                                                                   ggacgcCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                         <  2:4060697‑M2/134‑1 (MQ=255)

                                                                                                                       gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgg                     >  2:4100916/1‑140 (MQ=255)

                                                                                                                       gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgg                     >  2:3578843/1‑140 (MQ=255)

                                                                                                                       gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgg                     >  2:211895/1‑140 (MQ=255)

                                                                                                                        cccgcccAGCCGGACCTACAGGAGCGACCGGAGCGACCGGAGCTCCGTGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                    <  1:430878/135‑1 (MQ=18)

                                                                                                                        ccGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                    <  1:989913/140‑1 (MQ=255)

                                                                                                                        ccGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGCGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGTGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                    <  1:3216350/140‑1 (MQ=255)

                                                                                                                        ccGGACCAGCCGGAGCGACAGGAGCCACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                    <  1:5232927/140‑1 (MQ=255)

                                                                                                                        ccGGACCAGCCGGAGCGACAGGAGCCACAGGCGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGCAAGCCGGACCACCCGGGGCCCCGGGAGCGACAGGGGCTCCgggg                    >  1:4904989/1‑140 (MQ=18)

                                                                                                                            ccccgCCCGAGCCACACGAGCGCCCGGAGCCACCCGAGCCGCCGGCGACGCCGGACCAGGAGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                <  2:4991678/136‑1 (MQ=18)

                                                                                                                                 ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                >  1:2960270/1‑135 (MQ=255)

                                                                                                                                  cGGAGCTACAGGACCTCAAGGAGCCCAAGGAGCAACCGGCGAACCTGGACGTGCGGGAGGGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACTGGAGCCACAGGCGCTACTGGGGAAGCCGGAc          <  1:5520172/140‑1 (MQ=12)

                                                                                                                                     agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg       >  2:2538601/1‑140 (MQ=255)

                                                                                                                                          caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTCGGGAATCCGGACCAGCCGGa  >  1:4801259/1‑140 (MQ=255)

CTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGA  >  NZ_CP034943/607330‑607607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`