Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,484 G→C 6.7% A109A (GCG→GCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,4840GC 6.7% 480.1 / 5.7 213A109A (GCG→GCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (70/119);  new base C (4/13);  total (77/136)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

ACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAC  >  NZ_CP034943/607346‑607621
                                                                                                                                          |                                                                                                                                         
accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTCACGCGGAAGCCCCACGATCCcgagcgacagcagcgacagtagcga                                                                                                                                          >  2:3805876/1‑140 (MQ=17)
accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                                                                                          >  1:1478448/1‑140 (MQ=255)
 ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcggcaggagcgacaggagcgac                                                                                                                                         <  2:5797592/140‑1 (MQ=255)
  cagccggaccaGCCGTAGCGCCGGGAGCCACGGGAGCTCCCGGGGAATCCGGACCAGCCGGACCAACCGGAGCGAAAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgaca                                                                                                                                        <  2:3648281/140‑1 (MQ=14)
    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                                      <  1:3622896/140‑1 (MQ=255)
    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                                      <  1:4123909/140‑1 (MQ=255)
    gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                                                                                      >  2:1129840/1‑140 (MQ=255)
     ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGCCAGGAGATACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgaccgga                                                                                                                                     >  2:3699028/1‑140 (MQ=255)
        gcccccccGGCGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt                                                                                                                                  <  1:2653511/134‑1 (MQ=14)
             aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                                             >  2:3874885/1‑140 (MQ=255)
             aCCGGACCGCCCGGAGCCACGGGAGCAACCGGCGAACCCGGCCCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                                             <  1:5591824/140‑1 (MQ=14)
              ccGGTGGGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                            <  1:2401875/140‑1 (MQ=14)
              ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGCGCTCGCGGAGATACCGGGCCCGCCGCACCAGCCCGCGCGTCATGGGGGAGCGGTGCGACGGGAGCTACCggg                                                                                                                            >  1:2010368/1‑140 (MQ=9)
               cGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTCCCGGGGACGCCGGCCCAGCCGGGGCGACAGGAGCGCCAGGAGCGACCGGAGCGCCCgggg                                                                                                                           >  1:3845216/1‑140 (MQ=14)
                  aGCGTCCGGAGCCACGGGAGCTACCGGGGCAGCCGGACCAGCCTGACCTACCTGAGGTTCAGGAGGGACTGGAGCTACCGGGGAAGCCGGACCAGCCGTATCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                        <  2:1531214/140‑1 (MQ=14)
                    ccaCGGGAGCCCCGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCt                                                                                                                      <  1:2860032/138‑1 (MQ=255)
                      aCGGGAGCCCCCGGAGCTACCGGCGAAGCCGGACCACCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTgg                                                                                                                    <  1:2035557/140‑1 (MQ=14)
                       cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACCGGAGCTACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCCGAGCAGCTACCCGAGCGCCAGCAGAGGGCGGCGAAGCTggg                                                                                                                   >  1:986651/1‑139 (MQ=14)
                        gggAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCTGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTCCCGGGGAGGCGGGAc                                                                                                                  >  1:3270467/1‑140 (MQ=17)
                             ccccGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                             <  1:3734358/137‑1 (MQ=255)
                                 gggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                                                                                                   >  2:1070360/1‑130 (MQ=255)
                                    aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGa                                                                                                      >  1:4435359/1‑140 (MQ=255)
                                      cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                    >  1:2558354/1‑140 (MQ=255)
                                      cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGCCACCGGACCAGCCGGAGCTACAGCAGCTTCCGGAGCGACAGGAGCTAGCGGGGACGCTGGACCTGCTGGAgcggcg                                                                                                    >  1:3207068/1‑140 (MQ=14)
                                        actggggAAGCTGGACCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                  <  2:4292545/137‑1 (MQ=255)
                                        aCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                  >  1:4263012/1‑140 (MQ=255)
                                        aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                  <  1:671313/140‑1 (MQ=255)
                                              gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCCGGCGAAGCTGGAGCAGCCGGAGCGACAGGAGCTAc                                                                                            >  2:4827460/1‑140 (MQ=14)
                                              gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                            <  1:4090539/140‑1 (MQ=255)
                                                 gccggaccagccggaccaCCCGGAGCGACTGGAGCGACAGGATCGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                         <  2:2860378/140‑1 (MQ=17)
                                                 gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                         >  2:3345522/1‑140 (MQ=255)
                                                 gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCAACAGGCGAAGCAGGAGCAGCTGGAGCGACGGGAGCGACAgg                                                                                         >  2:4229818/1‑140 (MQ=17)
                                                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGAGCGACAGGAGCTACCGGGCAAGCAGGACCACCAGGAGCGACGGGAGCGACAGGAg                                                                                       >  1:3691526/1‑140 (MQ=14)
                                                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                       >  1:3494240/1‑140 (MQ=255)
                                                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                       >  1:1917128/1‑140 (MQ=255)
                                                   cggaccagccggaccaACCGCAGCGACAGGAGCGACAGGAGCTAACGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAGCTGGACCAGCTGGAGCGACTGGAGAGACAGGAg                                                                                       >  1:2330777/1‑140 (MQ=17)
                                                      accagccggaccaCCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa                                                                                    <  2:2076663/140‑1 (MQ=255)
                                                           ccggacccCCCCGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                               <  1:1467671/140‑1 (MQ=17)
                                                           ccggaccaACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGCGCGACAGGAGCGTCAGGAGCAACCGGATAAGCTGGAGCAGCTGGAGAGACGGGAGCGAGAGGAGCTACAggg                                                                               >  1:4832383/1‑140 (MQ=17)
                                                           ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                               >  2:2709703/1‑140 (MQ=255)
                                                                cccaCCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                          <  1:5577392/137‑1 (MQ=255)
                                                                ccaCCCGGAGCGACAGGAGCGACAGGCGCTCCCGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTCCCGGGGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                          <  1:1186450/140‑1 (MQ=18)
                                                                ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGCGACCGGAGCGGCCGGGGAAGCTGGCGCAGCGGGAGCGCCCGGAGCGCCCGGAGCTACGGGGGAAGc                                                                          >  2:1863386/1‑140 (MQ=17)
                                                                ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                          <  1:3524460/140‑1 (MQ=255)
                                                                    ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAGAGGAGCTACAGGAGAGACAGGACCTACCGGTGACGCTGGAGCAACCGCAGCGACGGGAGCGACAGGAGCGACCGGGGAGGCCggg                                                                      >  2:2189461/1‑139 (MQ=17)
                                                                     cggagcgaccggagcgacACGAGCTACCGGGGAAGCCGGACCCGCCGCAGCCTCAGGAGCGACACGAGCGACAGGAGATACCGGGCAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     <  2:616087/140‑1 (MQ=17)
                                                                     cGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     <  2:4893128/140‑1 (MQ=255)
                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     <  2:4703173/140‑1 (MQ=255)
                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     <  2:542192/140‑1 (MQ=255)
                                                                     cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                     <  1:3345522/140‑1 (MQ=255)
                                                                         gcgacaggagcgacaggaGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACCGGAGCGACCGGGGAAGCAGGACCCACTGGAGCGCCGGGAGCGACCGGAGCTACCGGGGCACCCGGagcagc                                                                 >  2:1552400/1‑140 (MQ=18)
                                                                         gcgacaggagcgacaggaGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                 <  1:5754841/140‑1 (MQ=255)
                                                                             caggagcgacaggagcgaCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggg                                                             <  1:3976639/140‑1 (MQ=255)
                                                                             caggagcgacaggagcTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGGGCGCCAGGCGCCACGGGCCACGCGGCGCCAGCTGGAGAGCCGGGCGCCAGAGGACCTACCGGGGACGCCGGAGCAGCgggg                                                             >  2:3138595/1‑140 (MQ=12)
                                                                              aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgggg                                                            <  1:3021384/140‑1 (MQ=255)
                                                                                gagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGACCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGcc                                                          <  2:937475/140‑1 (MQ=255)
                                                                                   cgcccggagcTACCGGGCCAGCCGGACCAGCCGGAGCGCCCGGAGCGACGGGAGCTACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                       <  2:2110435/135‑1 (MQ=14)
                                                                                   ccacaggagcTTGCGGCGAACCAGGACCACCCGGACCGCCAGGAGCGCCAGGACCGCCAGGAGCTCCCGGCGAAGCTGGACCCCCTGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                       <  1:4931517/138‑1 (MQ=17)
                                                                                     ccaggagcTCCCGGCGAAGCCGCACCAGCCGGAGCCACAGGAGCCACAGGAGCGACCGGTGCTACCGGCGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                     <  2:3726713/139‑1 (MQ=18)
                                                                                     ccaggagcGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                     <  2:3088444/139‑1 (MQ=255)
                                                                                     accggagcTACGGGTGAAGCGGGACCACCCGGAGGGCCAGGAGAGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                     <  1:3560325/137‑1 (MQ=17)
                                                                                     accggagcTACCGGCGAAGCCGGACCCGCCGGAGCCTCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                     <  1:5333737/137‑1 (MQ=18)
                                                                                     accggagcAACCGGGGAAGCCTGTGCAGCGGGATCTACAGGAGCGACTGGAGCCACCGGAGCCACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                     <  1:2710497/137‑1 (MQ=14)
                                                                                     acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                     <  1:4488812/140‑1 (MQ=255)
                                                                                         gccctcccGGTGAACCCGGACCAGCCGGAGCCCCAGGAGCGACAGGAGCCCCAGGAGCAACCGGTGAACCAGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                                 <  2:1435667/134‑1 (MQ=17)
                                                                                          agcTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGCGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                <  1:1691821/140‑1 (MQ=255)
                                                                                           gcTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                               <  1:686948/140‑1 (MQ=255)
                                                                                           gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGATACCGGGGAAGCTGGACCACCTCGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGAGCAGCCGGGGCCCCGGGCGCcac                                               >  1:4991678/1‑140 (MQ=18)
                                                                                           gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                               <  1:85794/140‑1 (MQ=255)
                                                                                            cTACCGGGGAAGCCGGCCCAGCCGGAGCCACCGCAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTTGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                              <  1:1768819/140‑1 (MQ=17)
                                                                                            cTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                              <  1:1025026/140‑1 (MQ=255)
                                                                                              acgggggAAGGCGGTGCAGCCGTAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                            <  1:5266158/137‑1 (MQ=21)
                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTACAGGAGAGAGCGGGGCAGCGGGAGCAGCAGGAGCGACGGGAGAGCCAGGAGCTACCGGAGAGCCCGGAGCAGCCGGGGCCACGGGAGCCCCAgg                                            >  2:1928037/1‑140 (MQ=18)
                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                            <  1:4909929/140‑1 (MQ=255)
                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCAACCGGCGACGCTGGACCAGCTGGCGCGACGGGAGCGACAGGAGCACCCGACGACTCGGGAACATCCGGATCAACGGGAGCCACAgg                                            >  1:5177064/1‑140 (MQ=17)
                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                            <  1:5666855/140‑1 (MQ=255)
                                                                                                cGGTGAAGCGGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGCAGCTGGACCAGCTGGAGCGCCGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAgggg                                          <  2:4353701/140‑1 (MQ=18)
                                                                                                cGGGGAAGGCGCACCCGCCGGAGGCGCAGGAGCGACAGGAGCGACAGGTGAGACTGGGCACGCTTGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                          <  2:4460342/140‑1 (MQ=18)
                                                                                                   ggAAGCCGGACCAGCCGGACCGACCGGCGCGACAGCAGCGACAGGAGCTACCGGGGAAGGTGGTCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                       <  1:5213291/140‑1 (MQ=18)
                                                                                                   ccaaGCCGGACCAGTCGGACGGCCCTGAGGGACCTGAGCGACAGGAGCTCCCGGTGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                       <  2:5717609/138‑1 (MQ=17)
                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                  >  2:4063878/1‑80 (MQ=255)
                                                                                                      aGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGTAGCGGGACCAGCAGGCCCACCGGGCGCCACAGGGGGTGCTg                                    >  1:1095278/1‑140 (MQ=17)
                                                                                                       gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgg                                   >  2:3578843/1‑140 (MQ=255)
                                                                                                        cccgcccAGCCGGACCTACAGGAGCGACCGGAGCGACCGGAGCTCCGTGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                  <  1:430878/135‑1 (MQ=18)
                                                                                                        ccGGACCAGCCGGAGCGACAGGAGCCACAGGCGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGCAAGCCGGACCACCCGGGGCCCCGGGAGCGACAGGGGCTCCgggg                                  >  1:4904989/1‑140 (MQ=18)
                                                                                                         cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgggg                                 >  2:4060537/1‑140 (MQ=255)
                                                                                                            ccccgCCCGAGCCACACGAGCGCCCGGAGCCACCCGAGCCGCCGGCGACGCCGGACCAGGAGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                              <  2:4991678/136‑1 (MQ=18)
                                                                                                            aCCAGCCGGAGGACCAGGACCCACAGGAGCTAAAGGAGCCACCGGGGAAGCAGGACCCGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCGGGGGCCACGGGAGCCACAGGCGCTACGGGGGAAg                              <  1:2244804/140‑1 (MQ=18)
                                                                                                            aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCGGCCGGGGCCCCGGGAGCCACAGGGGATCCTGGGGacg                              >  1:1170227/1‑138 (MQ=21)
                                                                                                            aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                              >  2:4581457/1‑140 (MQ=255)
                                                                                                            aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                              <  2:1322036/140‑1 (MQ=255)
                                                                                                            aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGCACCAGCTGGACCGACGGGACCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAg                              >  1:5486543/1‑140 (MQ=25)
                                                                                                                  cGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                        <  1:4605292/140‑1 (MQ=255)
                                                                                                                       cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                   <  1:4041040/140‑1 (MQ=255)
                                                                                                                         ccaggagcgcccggacccccaggcgcTCCCGGAGCAGCCGGACCGCCCGGAGCGACGGGAGCGACAGGAGCACCCGGGCAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                 <  2:2379160/139‑1 (MQ=17)
                                                                                                                          ccggcgcgacaggagcccccggagcTCCCGCGGACGCGGGACCAGCTGGAGGGACGGGAGCGACCGGAGCCCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                <  2:1265829/135‑1 (MQ=18)
                                                                                                                          caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTCGGGAATCCGGACCAGCCGGa                >  1:4801259/1‑140 (MQ=255)
                                                                                                                                    aggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACCGCGGACCAGCCGGAGCGACCGGAg      >  2:4244130/1‑140 (MQ=18)
                                                                                                                                        gcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTGCAGGCGCCGCCGGAGCAGCCGGAGGGACACGAGCtcc  >  2:5175978/1‑138 (MQ=18)
                                                                                                                                         cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                                                                                >  1:1647848/1‑45 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAC  >  NZ_CP034943/607346‑607621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑