Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 44,865 T→A 100% intergenic (‑46/‑455) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8650TA99.4% 652.5 / ‑4.1 173intergenic (‑46/‑455)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (58/114);  total (58/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

TCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAA  >  NZ_CP034943/44731‑45000
                                                                                                                                      |                                                                                                                                       
tCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTatcttt                                                                                                                                    <  2:1160136/140‑2 (MQ=255)
tCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTatcttt                                                                                                                                    <  2:1096955/140‑2 (MQ=255)
tCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTatcttt                                                                                                                                    <  2:5226269/140‑2 (MQ=255)
  tGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCATCCCAAGAGATGATTATTTGTTTAAATTATATTTatctttct                                                                                                                                  >  2:3796004/1‑137 (MQ=255)
              agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:3847429/140‑1 (MQ=255)
              agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:2965168/140‑1 (MQ=255)
              agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:5463304/140‑1 (MQ=255)
              agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                      <  1:5259606/140‑1 (MQ=255)
                 acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCCAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACaaaa                                                                                                                   >  2:183993/1‑140 (MQ=255)
                   gacgCAGTTCGATAGGAATAACTACACCTCCTAATTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACATTCTCCTCCCAAGAGATACTTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATc                                                                                                                 <  1:3542278/140‑1 (MQ=255)
                     cgCAATTCGATAGGAATAACTTCACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  1:3508778/140‑1 (MQ=255)
                     cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  1:3878891/140‑1 (MQ=255)
                     cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  1:2216173/140‑1 (MQ=255)
                     cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCt                                                                                                               <  2:5185193/140‑1 (MQ=255)
                      gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:2040437/140‑1 (MQ=255)
                      gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:3834535/140‑1 (MQ=255)
                      gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTAATTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                              <  2:2187130/140‑1 (MQ=255)
                             gATAGGAATAACTACATGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                       <  2:1014123/140‑1 (MQ=255)
                             gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                       <  1:4823416/140‑1 (MQ=255)
                             gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                       <  2:2072615/140‑1 (MQ=255)
                             gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCGTACCTTCa                                                                                                       <  2:1443882/140‑1 (MQ=255)
                               tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     <  1:5593970/140‑1 (MQ=255)
                               tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     <  2:942223/140‑1 (MQ=255)
                               tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     >  2:5545923/1‑140 (MQ=255)
                                aGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAg                                                                                                    <  2:4333060/140‑1 (MQ=255)
                                       aCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACa                                                                                             <  1:3112756/140‑1 (MQ=255)
                                         tACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATACACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAg                                                                                           <  1:3901557/140‑1 (MQ=255)
                                         tACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAg                                                                                           <  1:4729793/140‑1 (MQ=255)
                                           cacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGt                                                                                         >  1:5758602/1‑140 (MQ=255)
                                            acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCGTACCTTCATAGCCTAACAAGGtt                                                                                        <  2:1152565/140‑1 (MQ=255)
                                             cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     <  2:4421172/126‑1 (MQ=255)
                                             cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATa                                                                                                     >  1:4421172/1‑126 (MQ=255)
                                             cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:2288782/1‑140 (MQ=255)
                                             cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:3017411/1‑140 (MQ=255)
                                             cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:5241864/1‑140 (MQ=255)
                                                ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTcaa                                                                                    >  2:4564136/1‑138 (MQ=255)
                                                ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                    >  2:1932415/1‑140 (MQ=255)
                                                ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                    >  1:2804134/1‑140 (MQ=255)
                                                ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                    >  1:3980290/1‑140 (MQ=255)
                                                ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAACTTCATAGCATAACAAGGTTTCCa                                                                                    >  2:3040460/1‑140 (MQ=255)
                                                  tAGCTCATCAACTTTAAGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaat                                                                                  <  2:5663168/140‑1 (MQ=255)
                                                  tAGCTAATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaat                                                                                  <  2:3331459/140‑1 (MQ=255)
                                                        atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGAGTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                            >  1:2729851/1‑140 (MQ=255)
                                                         tcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTc                                                                           >  2:4617204/1‑140 (MQ=255)
                                                         tcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTc                                                                           >  2:5377496/1‑140 (MQ=255)
                                                          caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:4788209/1‑140 (MQ=255)
                                                          caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:633935/1‑140 (MQ=255)
                                                          caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:3776213/1‑140 (MQ=255)
                                                          caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:3371941/1‑140 (MQ=255)
                                                          caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:2724723/1‑140 (MQ=255)
                                                                  cGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                                  >  1:5405049/1‑140 (MQ=255)
                                                                    tACAATACCAGTAGATTTCCTCAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTAGATTTATCTTTCTCATTATTCGACCAAATCCTTACCGTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                <  1:1284016/140‑1 (MQ=255)
                                                                    tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                <  2:2447662/140‑1 (MQ=255)
                                                                    gaCAATACCAGTAGATTTCATAAACGTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                <  1:3800089/139‑1 (MQ=255)
                                                                       aaTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtttg                                                             <  2:3871775/140‑1 (MQ=255)
                                                                          aCCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTa                                                          <  1:948267/140‑1 (MQ=255)
                                                                            cAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTatt                                                        <  1:3776213/140‑1 (MQ=255)
                                                                            cAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTatt                                                        <  1:1469899/140‑1 (MQ=255)
                                                                            cAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTatt                                                        <  1:1733059/140‑1 (MQ=255)
                                                                              gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:1204894/140‑1 (MQ=255)
                                                                              gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:1155426/140‑1 (MQ=255)
                                                                              gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:4580943/140‑1 (MQ=255)
                                                                              gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:581481/140‑1 (MQ=255)
                                                                              gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  1:333241/140‑1 (MQ=255)
                                                                               tAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgat                                                     >  1:1476373/1‑140 (MQ=255)
                                                                                 gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgattg                                                   >  1:5578298/1‑140 (MQ=255)
                                                                                  aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                                  <  1:2165877/140‑1 (MQ=255)
                                                                                  aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                                  <  1:2584552/140‑1 (MQ=255)
                                                                                   tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 <  1:1485513/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCCAGTGATGATTATTTTTTTTAATTATTTTTATCTTTCTCATTCTTGGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:3627521/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:2032941/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:498342/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:3198143/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:2902130/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:1474761/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:833893/140‑1 (MQ=255)
                                                                                         aaaCTTTCTCCTCCAAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTAGTCGACAAAATCCTTACCTTCATAGCCTAACAAGGTTTCCAATAAGCGTCACTCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:1418035/140‑1 (MQ=255)
                                                                                           aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                         <  2:5758602/140‑1 (MQ=255)
                                                                                                 tcctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           >  1:355013/1‑132 (MQ=255)
                                                                                                 tcctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:355013/132‑1 (MQ=255)
                                                                                                 tcctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                   <  1:4589007/140‑1 (MQ=255)
                                                                                                  cctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCTTTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                  <  2:5387220/140‑1 (MQ=255)
                                                                                                  cctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                  <  2:4019966/140‑1 (MQ=255)
                                                                                                  cctccCAAGAGAAGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCAGAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                  <  2:5762500/140‑1 (MQ=255)
                                                                                                   ctccCAAGAGATGATTATTTGTTTACATTATATTGATCTTTCTCATTATTCGACAACATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                 <  2:3414105/140‑1 (MQ=255)
                                                                                                      ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                              <  1:5151361/140‑1 (MQ=255)
                                                                                                      ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                              <  1:2724723/140‑1 (MQ=255)
                                                                                                      ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                              <  1:1943276/140‑1 (MQ=255)
                                                                                                          gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:2288782/140‑1 (MQ=255)
                                                                                                          gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:2350804/140‑1 (MQ=255)
                                                                                                          gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:5610431/140‑1 (MQ=255)
                                                                                                            gatgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                        <  2:2637186/140‑1 (MQ=255)
                                                                                                            gatgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                        <  2:167219/140‑1 (MQ=255)
                                                                                                             atgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                       >  1:2264918/1‑140 (MQ=255)
                                                                                                             atgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                       >  1:3066512/1‑140 (MQ=255)
                                                                                                                    tttGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTc                                      <  2:1327055/118‑1 (MQ=255)
                                                                                                                        tttAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAact            <  2:1983543/140‑1 (MQ=255)
                                                                                                                           aaaTTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactact         <  1:633935/140‑1 (MQ=255)
                                                                                                                                  atTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATaa  >  2:1795657/1‑140 (MQ=255)
                                                                                                                                  atTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGAATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGATCTTATAGTCAGTAATGTATCAGAAACTACTCaaaaaa  >  2:2982734/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TCTGCGATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAA  >  NZ_CP034943/44731‑45000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: