Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,499 G→C 8.6% G114G (GGG→GGC EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,4990GC 8.6% 520.5 / 2.4 233G114G (GGG→GGCEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (74/125);  new base C (9/13);  total (87/146)
Fisher's exact test for biased strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

CCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCA  >  NZ_CP034943/607360‑607637
                                                                                                                                           |                                                                                                                                          
ccGGTGGGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                                            <  1:2401875/140‑1 (MQ=14)
ccGGAGCCCCGGGACCCCCGGGAGCTCCCGGGGAAGCCGGACCCGCAGCACCAACCGGAGCGACTGGTGCGACAGGAGCTACCGGGGAAGCCGGTCCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                                            <  1:2657074/140‑1 (MQ=14)
 cGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTCCCGGGGACGCCGGCCCAGCCGGGGCGACAGGAGCGCCAGGAGCGACCGGAGCGCCCgggg                                                                                                                                           >  1:3845216/1‑140 (MQ=14)
 cGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGACACAGGAGCTAACgcgg                                                                                                                                           >  1:3582396/1‑140 (MQ=17)
    agccccGGGAGCCACGGGATGAACCGGGCATCCCGGACCAGCCGTACCAAACGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                        <  2:3674060/135‑1 (MQ=14)
         cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCACGAGCGACAGGAGCGAGGGGGGGGAGAGGAGCTAGGGGGGACGCTggg                                                                                                                                   >  2:1065125/1‑139 (MQ=17)
               ccccGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                             <  1:3734358/137‑1 (MQ=255)
                       gCTACCGGGCAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGAc                                                                                                                     <  1:1822115/140‑1 (MQ=255)
                        cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                    >  1:351750/1‑140 (MQ=255)
                        cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                    >  1:1322036/1‑140 (MQ=255)
                        cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGCCACCGGACCAGCCGGAGCTACAGCAGCTTCCGGAGCGACAGGAGCTAGCGGGGACGCTGGACCTGCTGGAgcggcg                                                                                                                    >  1:3207068/1‑140 (MQ=14)
                          aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                  >  2:3956870/1‑140 (MQ=255)
                          aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                  <  2:1540279/140‑1 (MQ=255)
                             ggggAAGCCGGCCCAGCCGGACCACCCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGc                                                                                                               <  1:5406854/140‑1 (MQ=255)
                               cgAACCCGGACCAGACGGACAACCCGGAGGTAAAGGAGCGACAGGAGCTACCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                                                                                                             <  2:2737846/139‑1 (MQ=17)
                                gcagccggaccagccggacaCCCGGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                            <  2:4329642/138‑1 (MQ=17)
                                gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCCGGCGAAGCTGGAGCAGCCGGAGCGACAGGAGCTAc                                                                                                            >  2:4827460/1‑140 (MQ=14)
                                gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                            <  1:4090539/140‑1 (MQ=255)
                                   gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                         <  2:4446114/140‑1 (MQ=255)
                                   gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTGCCGGGGAAGCTGGAGCAGCAGGAGCGACGGGAGCGACCgg                                                                                                         >  1:2027435/1‑140 (MQ=255)
                                   gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                         >  2:3345522/1‑140 (MQ=255)
                                     cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                       >  1:1986399/1‑140 (MQ=255)
                                     cggaccagccggaccaACCGCAGCGACAGGAGCGACAGGAGCTAACGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAGCTGGACCAGCTGGAGCGACTGGAGAGACAGGAg                                                                                                       >  1:2330777/1‑140 (MQ=17)
                                             ccggaccaACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGCGCGACAGGAGCGTCAGGAGCAACCGGATAAGCTGGAGCAGCTGGAGAGACGGGAGCGAGAGGAGCTACAggg                                                                                               >  1:4832383/1‑140 (MQ=17)
                                                  cccaCCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                          <  1:5577392/137‑1 (MQ=255)
                                                  ccaCCCGGAGCGACAGGAGCGACAGGCGCTCCCGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTCCCGGGGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                          <  1:1186450/140‑1 (MQ=18)
                                                  ccaACCGGAGCGACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCCTCCGGAGCTACAGGAGCGACCGGAGCGACCGCAGCTACCGGGGAAGCAGGAGCAGCCGGAGCGACCGGAGCAACAGGAGCTACCGGGGAAGc                                                                                          >  2:4202255/1‑140 (MQ=14)
                                                  ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                          <  1:3221175/140‑1 (MQ=255)
                                                      ccGGAGCGACAGGATCTACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCGACAGGAGAGACAGTACAAACCGGGGACGCTGGAGCAGCTGGCGCGACGGGAGCGACAGGAGCGACAGGGGACGCCGGa                                                                                      >  2:5387497/1‑140 (MQ=17)
                                                      ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                      >  2:4461020/1‑140 (MQ=255)
                                                       cggagcgaccggagcgacACGAGCTACCGGGGAAGCCGGACCCGCCGCAGCCTCAGGAGCGACACGAGCGACAGGAGATACCGGGCAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                     <  2:616087/140‑1 (MQ=17)
                                                       cGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                     <  2:4893128/140‑1 (MQ=255)
                                                           gcgacaggagcgacaggaGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                 >  1:1718390/1‑140 (MQ=255)
                                                           gcgacaggagcgacaggaGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                 <  1:5754841/140‑1 (MQ=255)
                                                           ccgacaggacccccaggagTTACTGGTGAAGCCGGACCAGACGGACCCCCAGGAGCGACAGGCGCAAAAGGAGCACCCGGGGAAGCTGGACAAGCTGGAGCGACGGGAGCGACAGGAGATACCGGGGAAGCCGGACCAGc                                                                                 <  2:2048383/139‑1 (MQ=11)
                                                               caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGCCGGGAGCTACCGGCGAAGCGGGACCAGCTGGGGCGGCGGGAGCGTCAGGAGCTAGCGGGGGCGCCGGGGCAGCCggg                                                                             >  2:2564530/1‑140 (MQ=17)
                                                               caggagcgacaggagcTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGGGCGCCAGGCGCCACGGGCCACGCGGCGCCAGCTGGAGAGCCGGGCGCCAGAGGACCTACCGGGGACGCCGGAGCAGCgggg                                                                             >  2:3138595/1‑140 (MQ=12)
                                                                aggcgcgacaggagcTAGCGGGGATGCGGGACCACCCGGAGCGACAGGAGCCACAGGAGCGAAAGGACCTACCGGGGAAGATGGACCTGCTGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgggg                                                                            <  1:4283248/140‑1 (MQ=18)
                                                                  gcgccccaggagcTACCGGGGAAGCGGGACCAGCCGGAGGGACAGGCGGGACTGGAGCGTCAGGTGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGTCAGGAGCTACCGGGGAAGCCGGACCATCCGGGGcc                                                                          <  2:4610875/134‑1 (MQ=17)
                                                                     cgcccggagcTACCGGGCCAGCCGGACCAGCCGGAGCGCCCGGAGCGACGGGAGCTACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                       <  2:2110435/135‑1 (MQ=14)
                                                                       ccaggagcTCCCGCCGAACCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                     <  2:1071328/139‑1 (MQ=18)
                                                                       ccaggagcGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                     <  2:3088444/139‑1 (MQ=255)
                                                                       accggagcTACGGGTGAAGCGGGACCACCCGGAGGGCCAGGAGAGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                     <  1:3560325/137‑1 (MQ=17)
                                                                       accggagcTACCGGCGAAGCCGGACCCGCCGGAGCCTCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                     <  1:5333737/137‑1 (MQ=18)
                                                                       accggagcAACCGGGGAAGCCTGTGCAGCGGGATCTACAGGAGCGACTGGAGCCACCGGAGCCACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                     <  1:2710497/137‑1 (MQ=14)
                                                                       acaggagcGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCTACAGGAGCTTCCGGGGCCGCTGGAGCTCCGGGCGCGCCGGGGGCGGCGGGCGCTCCCGGGGACGCCGGGCCGGCGGGGGccggggg                                                                     >  2:823328/1‑135 (MQ=12)
                                                                           gccctcccGGTGAACCCGGACCAGCCGGAGCCCCAGGAGCGACAGGAGCCCCAGGAGCAACCGGTGAACCAGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                                                 <  2:1435667/134‑1 (MQ=17)
                                                                            agcgcccGGGGCTGCCGGACCAGCCGGCGCGACAGGAGCCACCGGAGCGACAGGCGCTCCGGGGCAAGCGGGACCAGCGGGAGGGAGGGGCGCCACTGGAGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                                <  1:5061508/135‑1 (MQ=16)
                                                                             gcTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                               <  1:686948/140‑1 (MQ=255)
                                                                             gcTCCCGGCGAAGCCGGACCACCCGGAGCCACAGGACCGACAGGAGCCACAGGAGCTACAGGCGAAGCTGGACGACCGGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                               <  1:77799/140‑1 (MQ=18)
                                                                             gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGATACCGGGGAAGCTGGACCACCTCGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGAGCAGCCGGGGCCCCGGGCGCcac                                                               >  1:4991678/1‑140 (MQ=18)
                                                                             gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                               <  1:85794/140‑1 (MQ=255)
                                                                              cTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                              <  1:1025026/140‑1 (MQ=255)
                                                                              cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                              <  1:1169036/140‑1 (MQ=255)
                                                                               caCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAg                                                             <  1:3423153/139‑1 (MQ=255)
                                                                                cccGGGGAAGCCGGACCAGCCGGAGCGACAGGACCGCAAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:4322929/139‑1 (MQ=255)
                                                                                aCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:1492604/140‑1 (MQ=255)
                                                                                aCCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:4007721/140‑1 (MQ=255)
                                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTACAGGAGAGAGCGGGGCAGCGGGAGCAGCAGGAGCGACGGGAGAGCCAGGAGCTACCGGAGAGCCCGGAGCAGCCGGGGCCACGGGAGCCCCAgg                                                            >  2:1928037/1‑140 (MQ=18)
                                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:4909929/140‑1 (MQ=255)
                                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:1766800/140‑1 (MQ=255)
                                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:4461020/140‑1 (MQ=255)
                                                                                aCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                            <  1:635986/140‑1 (MQ=255)
                                                                                 ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCAGGACCAGCTGGAGCGACGGCAGCGACAGGAGCTAACGGCGAAGCAGGACCAGCCGGGGCGACGGGAGCCACAggg                                                           >  1:4502141/1‑140 (MQ=21)
                                                                                  cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                                            <  1:4923980/58‑1 (MQ=255)
                                                                                  cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                                                            >  2:4923980/1‑58 (MQ=255)
                                                                                  cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                          <  1:5617730/140‑1 (MQ=255)
                                                                                     ggcaGCCGGCCCAGCCGGAGCTCCCGGAGCCACAGGAGCGCCCGGAGCACCCGGCGAAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                       <  2:2213563/137‑1 (MQ=18)
                                                                                     ggAAGCGGCACCAGCCGGACCGCCCGGAGCGTCATCAGCGACAGGAGCACACGGCGAAGCAGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                       <  2:5717664/140‑1 (MQ=17)
                                                                                     ggAAGCCGGACCAGCCGGACCGACCGGCGCGACAGCAGCGACAGGAGCTACCGGGGAAGGTGGTCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                       <  1:5213291/140‑1 (MQ=18)
                                                                                      gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                  <  1:4063878/80‑1 (MQ=255)
                                                                                      gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                  >  2:4063878/1‑80 (MQ=255)
                                                                                        aGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGTAGCGGGACCAGCAGGCCCACCGGGCGCCACAGGGGGTGCTg                                                    >  1:1095278/1‑140 (MQ=17)
                                                                                         gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgg                                                   >  2:211895/1‑140 (MQ=255)
                                                                                          ccGGACCAGCCGGAGCGACAGGAGCCACAGGCGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGCAAGCCGGACCACCCGGGGCCCCGGGAGCGACAGGGGCTCCgggg                                                  >  1:4904989/1‑140 (MQ=18)
                                                                                           cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGGCAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGTAGCGACCGCAGCGACCGGGGAAGCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGCGACgggcg                                                 >  2:4289773/1‑138 (MQ=21)
                                                                                              aCCAGCCGGAGGACCAGGACCCACAGGAGCTAAAGGAGCCACCGGGGAAGCAGGACCCGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCGGGGGCCACGGGAGCCACAGGCGCTACGGGGGAAg                                              <  1:2244804/140‑1 (MQ=18)
                                                                                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                              <  2:351750/140‑1 (MQ=255)
                                                                                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                              <  2:1322036/140‑1 (MQ=255)
                                                                                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGacg                                              >  1:5234176/1‑138 (MQ=255)
                                                                                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGCACCAGCTGGACCGACGGGACCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAg                                              >  1:5486543/1‑140 (MQ=25)
                                                                                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGGGCCACGGGAGCCCCAGGGGCTACTGGGGAAGCCGGa                                         >  2:1491148/1‑140 (MQ=255)
                                                                                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                              >  1:2960270/1‑135 (MQ=255)
                                                                                                        gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                    <  1:2538601/140‑1 (MQ=255)
                                                                                                         ctacaggagcgacaggagcgacaggagcTACCGGGGATGGTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                   <  1:3699028/138‑1 (MQ=255)
                                                                                                            ccggcgcgacaggagcccccggagcTCCCGCGGACGCGGGACCAGCTGGAGGGACGGGAGCGACCGGAGCCCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                <  2:1265829/135‑1 (MQ=18)
                                                                                                            caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTCGGGAATCCGGACCAGCCGGa                                >  1:4801259/1‑140 (MQ=255)
                                                                                                                      aggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACCGCGGACCAGCCGGAGCGACCGGAg                      >  2:4244130/1‑140 (MQ=18)
                                                                                                                         agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCtc                   >  1:2862608/1‑139 (MQ=18)
                                                                                                                          gcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCtcc                  >  2:377693/1‑138 (MQ=255)
                                                                                                                          gcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTGCAGGCGCCGCCGGAGCAGCCGGAGGGACACGAGCtcc                  >  2:5175978/1‑138 (MQ=18)
                                                                                                                           cgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                                                                                                >  1:1647848/1‑45 (MQ=255)
                                                                                                                             actggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg               <  1:4194359/137‑1 (MQ=255)
                                                                                                                             acagtagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGCAGCGACAGGAGCTACCgg               >  1:330622/1‑140 (MQ=255)
                                                                                                                               aggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg             >  2:4784637/1‑140 (MQ=255)
                                                                                                                               aggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg             >  2:566100/1‑140 (MQ=255)
                                                                                                                                       acGGCGAAGCTGGACCACCGGGAGTGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa     <  2:1638351/139‑1 (MQ=17)
                                                                                                                                        cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc    <  1:211895/140‑1 (MQ=255)
                                                                                                                                        cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc    <  1:4100916/140‑1 (MQ=255)
                                                                                                                                          gggAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCa  <  2:1718390/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCA  >  NZ_CP034943/607360‑607637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: