Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,514 T→G 8.3% A119A (GCT→GCG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5140TG 8.3% 452.1 / 5.2 233A119A (GCT→GCGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base T (75/120);  new base G (7/20);  total (89/144)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

CCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGA  >  NZ_CP034943/607375‑607652
                                                                                                                                           |                                                                                                                                          
ccccGGGAGCTACCGGGGAAGCCGGACCAGCCGGCCCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                            <  1:3734358/137‑1 (MQ=255)
ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                            <  1:1129840/140‑1 (MQ=255)
       aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGa                                                                                                                                     >  1:4435359/1‑140 (MQ=255)
         cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                                   >  1:351750/1‑140 (MQ=255)
           actggggAAGCTGGACCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 <  2:4292545/137‑1 (MQ=255)
           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCGGGACCAGCAGGAGCTACcgg                                                                                                                                 >  1:4147357/1‑140 (MQ=11)
           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 <  2:1540279/140‑1 (MQ=255)
           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 <  1:671313/140‑1 (MQ=255)
             cGGGGAAGCCGGACCAGCCGGAGCACCCGGAGCGACAGGAGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                                                                                                               <  2:4789098/140‑1 (MQ=255)
              ggggAAGCCGGCCCAGCCGGACCACCCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGc                                                                                                                              <  1:5406854/140‑1 (MQ=255)
              ggggAAGCCGGACCAGCCGGACAAACCGGATCGACATGAGCCACAGGAGCTCCGGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACAGGCGCTACCGGGGCAGCTGGAGCAGCGGGAGCGCCgggggg                                                                                                                              >  1:1196187/1‑137 (MQ=14)
                 gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCCGGCGAAGCTGGAGCAGCCGGAGCGACAGGAGCTAc                                                                                                                           >  2:4827460/1‑140 (MQ=14)
                    gccggaccagccggaccaCCCGGAGCGACTGGAGCGACAGGATCGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                        <  2:2860378/140‑1 (MQ=17)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGCGCACCCGGGGAAGCCGGACCAGCCTGAGCGACAGGAGCGACCGGAGCTACAGGAGCTACCGCACACGCGGCACCCCCTGGagcgagaggagcgagagg                                                                                                                        >  1:4383925/1‑140 (MQ=14)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                        <  2:4446114/140‑1 (MQ=255)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTGCCGGGGAAGCTGGAGCAGCAGGAGCGACGGGAGCGACCgg                                                                                                                        >  1:2027435/1‑140 (MQ=255)
                      cggaccagccggaccaACCGCAGCGACAGGAGCGACAGGAGCTAACGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAGCTGGACCAGCTGGAGCGACTGGAGAGACAGGAg                                                                                                                      >  1:2330777/1‑140 (MQ=17)
                        gaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCt                                                                                                                    <  2:3725382/140‑1 (MQ=255)
                         ccccgccggccccACCGGACCCACCGGAGGGACTGGCGCTCCGGGCGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                   <  2:136002/136‑1 (MQ=14)
                             cccggacccCCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGTCAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCgg                                                                                                               <  2:4579910/139‑1 (MQ=17)
                              ccggacccCCCCGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                              <  1:1467671/140‑1 (MQ=17)
                              ccggaccaACCGGAGGGACCGGAGACAGGGGAGCTACCGGAGCAGCCGGCCCAGCCGGACCCCCCGGTGCGACAGGAGCCACCGGAGCTACCGGGGAAGCTGGACCACCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                              <  1:2675504/140‑1 (MQ=11)
                              ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                              >  2:2709703/1‑140 (MQ=255)
                                   ccaCCCGGAGCCCCAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGACCGACAGGAGCGACAGGAGCCACAGGAGCTACCGGCGAAGCTGGACGAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  1:5577551/140‑1 (MQ=17)
                                   ccaACCGGAGCGACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCCTCCGGAGCTACAGGAGCGACCGGAGCGACCGCAGCTACCGGGGAAGCAGGAGCAGCCGGAGCGACCGGAGCAACAGGAGCTACCGGGGAAGc                                                                                                         >  2:4202255/1‑140 (MQ=14)
                                   ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  1:3357409/140‑1 (MQ=255)
                                   ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  1:3221175/140‑1 (MQ=255)
                                       ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAGAGGAGCTACAGGAGAGACAGGACCTACCGGTGACGCTGGAGCAACCGCAGCGACGGGAGCGACAGGAGCGACCGGGGAGGCCggg                                                                                                     >  2:2189461/1‑139 (MQ=17)
                                       ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAAGCTGGACAAGCTGGAGAGACGGGACCCACAGGAGCTACCGGGGAAGcctca                                                                                                     >  1:571924/1‑137 (MQ=18)
                                        cGGCGCGCCCGGAGCGCCCGGAGCTACCGGGGAAGCCGGACCAGCCGCAGCGACCGGAGCGACAGGAGGGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  1:2729035/140‑1 (MQ=17)
                                        cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  2:4703173/140‑1 (MQ=255)
                                        cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  2:542192/140‑1 (MQ=255)
                                        cGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  2:1170519/140‑1 (MQ=255)
                                        cGGAGCGACAGCCGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCGACAGTAGCGACTGGATCGACAGGTGCGCCCGTTGACGCTGGCCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  1:3536371/140‑1 (MQ=17)
                                            ccgacaggacccccaggagTTACTGGTGAAGCCGGACCAGACGGACCCCCAGGAGCGACAGGCGCAAAAGGAGCACCCGGGGAAGCTGGACAAGCTGGAGCGACGGGAGCGACAGGAGATACCGGGGAAGCCGGACCAGc                                                                                                <  2:2048383/139‑1 (MQ=11)
                                             cgaccggagcgaccggagCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                               <  1:3812973/140‑1 (MQ=255)
                                                caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACCGGAGCAACCGGGGAAGCGGGCCCAGCGGGAGCGACGGGCGCAACAGGAGCACCCGGGGCCGCCGGCCCGGCCggg                                                                                            >  2:1630738/1‑140 (MQ=17)
                                                   tcgcgacaggcgcTACCGGGGCAGCGGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGcc                                                                                         <  2:4275627/138‑1 (MQ=17)
                                                     gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                       >  2:4909929/1‑140 (MQ=255)
                                                      ctactggagcTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                      <  2:2111632/135‑1 (MQ=255)
                                                      cgacaggcgcTACCGCGGAAGCCGGACCAGCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTCGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                      <  2:3511770/140‑1 (MQ=18)
                                                      ccacaggagcTTGCGGCGAACCAGGACCACCCGGACCGCCAGGAGCGCCAGGACCGCCAGGAGCTCCCGGCGAAGCTGGACCCCCTGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                      <  1:4931517/138‑1 (MQ=17)
                                                        ccaggagcGACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  2:3088444/139‑1 (MQ=255)
                                                        accggagcAACCGGGGAAGCCTGTGCAGCGGGATCTACAGGAGCGACTGGAGCCACCGGAGCCACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  1:2710497/137‑1 (MQ=14)
                                                        acaggagcGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCTACAGGAGCTTCCGGGGCCGCTGGAGCTCCGGGCGCGCCGGGGGCGGCGGGCGCTCCCGGGGACGCCGGGCCGGCGGGGGccggggg                                                                                    >  2:823328/1‑135 (MQ=12)
                                                             agcgcccGGGGCTGCCGGACCAGCCGGCGCGACAGGAGCCACCGGAGCGACAGGCGCTCCGGGGCAAGCGGGACCAGCGGGAGGGAGGGGCGCCACTGGAGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                                               <  1:5061508/135‑1 (MQ=16)
                                                             agcTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGCGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCca                                                                               <  1:1691821/140‑1 (MQ=255)
                                                              gcTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              <  1:686948/140‑1 (MQ=255)
                                                              gcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGCGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              <  1:4804359/140‑1 (MQ=255)
                                                               cccccGGGGAAGCCGGACCAGCCGGACCGACAGGCGCGACAGGAGCGACAGGAGCTACCGGGGAATCTGGAACAGCTGGAGCGACGGGAGCGACAGGAGATACCGGGGAATCCGGACCAGCCGGCGCCACGGGAGCcaca                                                                             <  1:5512082/137‑1 (MQ=18)
                                                               cTACCGGGGAAGCCGGCCCAGCCGGAGCCACCGCAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTTGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:1768819/140‑1 (MQ=17)
                                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:1025026/140‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:1492604/140‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:4007721/140‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTACAGGAGAGAGCGGGGCAGCGGGAGCAGCAGGAGCGACGGGAGAGCCAGGAGCTACCGGAGAGCCCGGAGCAGCCGGGGCCACGGGAGCCCCAgg                                                                           >  2:1928037/1‑140 (MQ=18)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:6183/140‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:4909929/140‑1 (MQ=255)
                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCAGGACCAGCTGGAGCGACGGCAGCGACAGGAGCTAACGGCGAAGCAGGACCAGCCGGGGCGACGGGAGCCACAggg                                                                          >  1:4502141/1‑140 (MQ=21)
                                                                   cGGTGAAGCGGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGCAGCTGGACCAGCTGGAGCGCCGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAgggg                                                                         <  2:4353701/140‑1 (MQ=18)
                                                                   cGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAAGCTGGACCCGCTGGAGCGACGGGAGCGACCGGAGCAACCGGCGAAGCCGGACCAGCCGGAGCCGCCGTCGCCGCAgggg                                                                         >  2:1269276/1‑140 (MQ=17)
                                                                   cGGGGAAGGCGCACCCGCCGGAGGCGCAGGAGCGACAGGAGCGACAGGTGAGACTGGGCACGCTTGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                         <  2:4460342/140‑1 (MQ=18)
                                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                         <  1:5617730/140‑1 (MQ=255)
                                                                      ggacgcCGGACCCGACGGACCCACAGGCCCGACAGGAGCGAGAGGAGCTACCGGGGATGCTTGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:1953606‑M2/134‑1 (MQ=255)
                                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGc                                                                                                                     <  2:681854/93‑1 (MQ=255)
                                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGCGCAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGcga                                                                      >  2:5051264/1‑138 (MQ=21)
                                                                      ggAAGCCGGACCAGCCGGACCGACCGGCGCGACAGCAGCGACAGGAGCTACCGGGGAAGGTGGTCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:5213291/140‑1 (MQ=18)
                                                                      ccaaGCCGGACCAGTCGGACGGCCCTGAGGGACCTGAGCGACAGGAGCTCCCGGTGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  2:5717609/138‑1 (MQ=17)
                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 <  1:4063878/80‑1 (MQ=255)
                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 >  2:4063878/1‑80 (MQ=255)
                                                                           cccgcccAGCCGGACCTACAGGAGCGACCGGAGCGACCGGAGCTCCGTGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                 <  1:430878/135‑1 (MQ=18)
                                                                           ccGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                 <  1:989913/140‑1 (MQ=255)
                                                                            cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgggg                                                                >  2:4060537/1‑140 (MQ=255)
                                                                               aCCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                             <  1:3072406/140‑1 (MQ=255)
                                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggggccg                                                             >  2:3316160/1‑137 (MQ=255)
                                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCGGCCGGGGCCCCGGGAGCCACAGGGGATCCTGGGGacg                                                             >  1:1170227/1‑138 (MQ=21)
                                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGacg                                                             >  1:5234176/1‑138 (MQ=255)
                                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGCACCAGCTGGACCGACGGGACCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAg                                                             >  1:5486543/1‑140 (MQ=25)
                                                                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                             >  1:2960270/1‑135 (MQ=255)
                                                                                          ctacaggagcgacaggagcgacaggagcTACCGGGGATGGTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                  <  1:3699028/138‑1 (MQ=255)
                                                                                          cgacaggcgcgacaggcgcgacaggagCCCCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                  <  2:1478448/140‑1 (MQ=255)
                                                                                            ccaggagcgcccggacccccaggcgcTCCCGGAGCAGCCGGACCGCCCGGAGCGACGGGAGCGACAGGAGCACCCGGGCAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                <  2:2379160/139‑1 (MQ=17)
                                                                                            acaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                <  1:4904051/140‑1 (MQ=255)
                                                                                                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCCACTGGCGCTACAGGCGCAGCCGCACCAGCCGCAGCCACAgc                                       >  1:5275327/1‑139 (MQ=18)
                                                                                                          cgccccaggagcGACCGGGGAACCGGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                  <  1:4084137/135‑1 (MQ=18)
                                                                                                           gcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCtcc                                 >  2:377693/1‑138 (MQ=255)
                                                                                                           gcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTGCAGGCGCCGCCGGAGCAGCCGGAGGGACACGAGCtcc                                 >  2:5175978/1‑138 (MQ=18)
                                                                                                              ccccgagcCACCGGCGAAGCTGGACCCGCTGGCGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGATACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                              <  1:1738507/136‑1 (MQ=18)
                                                                                                              actggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                              <  1:4194359/137‑1 (MQ=255)
                                                                                                              actggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                              <  1:2287317/137‑1 (MQ=255)
                                                                                                              acagtagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCAGCAGCGACAGGAGCTACCgg                              >  1:330622/1‑140 (MQ=255)
                                                                                                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACGGGGGACGCCGGACCAGCCGGAGCGACGGGAGCGACAGCGGCTACTCGTCACCCCGGACCCGCGGCACCGACAGGAGCtcgctg                              >  1:845438/1‑135 (MQ=14)
                                                                                                                         cgcggAAGCTGGACCAGGTGGAGCGACGGGAGCGAGAGGTGATACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                   <  2:571924/137‑1 (MQ=18)
                                                                                                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                   <  1:211895/140‑1 (MQ=255)
                                                                                                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                   <  1:3578843/140‑1 (MQ=255)
                                                                                                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                   <  1:4100916/140‑1 (MQ=255)
                                                                                                                         aggggccgCTGGACTTGCTTGAGCTAGTGGAGCCAAAGGAGCTACCGGGGTAGCCGGACCCCCCGGTGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                   <  2:3183467/133‑1 (MQ=14)
                                                                                                                           gggAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCa                 <  2:1718390/140‑1 (MQ=255)
                                                                                                                                  tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAg          >  1:2546090/1‑140 (MQ=255)
                                                                                                                                      ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGac      <  1:4581457‑M1/140‑3 (MQ=255)
                                                                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa  >  1:4847723/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGA  >  NZ_CP034943/607375‑607652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: