Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,562 G→C 7.4% G135G (GGG→GGC EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5620GC 7.4% 485.9 / 2.9 211G135G (GGG→GGCEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (68/118);  new base C (2/11);  total (76/135)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

CAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607423‑607701
                                                                                                                                           |                                                                                                                                           
caggagcgacaggagcgaCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggg                                                                                                                                             <  1:3976639/140‑1 (MQ=255)
caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGCCGGGAGCTACCGGCGAAGCGGGACCAGCTGGGGCGGCGGGAGCGTCAGGAGCTAGCGGGGGCGCCGGGGCAGCCggg                                                                                                                                             >  2:2564530/1‑140 (MQ=17)
caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGCGACCGGAGCAACCGGGGAAGCGGGCCCAGCGGGAGCGACGGGCGCAACAGGAGCACCCGGGGCCGCCGGCCCGGCCggg                                                                                                                                             >  2:1630738/1‑140 (MQ=17)
   gcgccccaggagcTACCGGGGAAGCGGGACCAGCCGGAGGGACAGGCGGGACTGGAGCGTCAGGTGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGTCAGGAGCTACCGGGGAAGCCGGACCATCCGGGGcc                                                                                                                                          <  2:4610875/134‑1 (MQ=17)
      ctactggagcTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                                                                       <  2:2111632/135‑1 (MQ=255)
        tgcggagcTCACGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                     <  1:4520442/137‑1 (MQ=17)
        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                     <  1:4488812/140‑1 (MQ=255)
        acaggagcGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCTACAGGAGCTTCCGGGGCCGCTGGAGCTCCGGGCGCGCCGGGGGCGGCGGGCGCTCCCGGGGACGCCGGGCCGGCGGGGGccggggg                                                                                                                                     >  2:823328/1‑135 (MQ=12)
            gccctcccGGTGAACCCGGACCAGCCGGAGCCCCAGGAGCGACAGGAGCCCCAGGAGCAACCGGTGAACCAGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                                                                                                                 <  2:1435667/134‑1 (MQ=17)
              gcTCCCGGGGAAGCAGGACCACCCGGAGCAACAGGAGCGCCAGGAGCGCCAGGCGCTACCGGTCAAGCTGGACCTGCGGGAGAGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                                                               <  1:4151289/140‑1 (MQ=18)
              gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGATACCGGGGAAGCTGGACCACCTCGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGAGCAGCCGGGGCCCCGGGCGCcac                                                                                                                               >  1:4991678/1‑140 (MQ=18)
               cTACCGGGGAAGCCGGCCCAGCCGGAGCCACCGCAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTTGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              <  1:1768819/140‑1 (MQ=17)
               cTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              <  1:1025026/140‑1 (MQ=255)
               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              <  1:1169036/140‑1 (MQ=255)
                caCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAg                                                                                                                             <  1:3423153/139‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                            <  1:1492604/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                            <  1:4909200/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                            <  1:1170859/140‑1 (MQ=255)
                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                            <  1:6183/140‑1 (MQ=255)
                   cgcgcacgCCGCACCCGCCGGCCCCCCAGGACCGACCGGAGCGCCCGGAGCGACCGGGGAAGCTGGACCAGCGGCAGCCACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                          <  2:195653/133‑1 (MQ=17)
                   cGGGGAAGGCGCACCCGCCGGAGGCGCAGGAGCGACAGGAGCGACAGGTGAGACTGGGCACGCTTGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                          <  2:4460342/140‑1 (MQ=18)
                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                                                          <  1:5617730/140‑1 (MQ=255)
                      ggcaGCCGGCCCAGCCGGAGCTCCCGGAGCCACAGGAGCGCCCGGAGCACCCGGCGAAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                       <  2:2213563/137‑1 (MQ=18)
                      ggacgcCGGACCCGACGGACCCACAGGCCCGACAGGAGCGAGAGGAGCTACCGGGGATGCTTGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                       <  1:1953606‑M2/134‑1 (MQ=255)
                      ggAAGCGGCACCAGCCGGACCGCCCGGAGCGTCATCAGCGACAGGAGCACACGGCGAAGCAGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                       <  2:5717664/140‑1 (MQ=17)
                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGCGCAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGcga                                                                                                                       >  2:5051264/1‑138 (MQ=21)
                      ggAAGCCGGACCAGCCGGACCGACCGGCGCGACAGCAGCGACAGGAGCTACCGGGGAAGGTGGTCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                       <  1:5213291/140‑1 (MQ=18)
                      cgcacccGGACCCCCCGGAGCCAGCGGAGCCACAGGAGCGACAGGAGCTCCCGGACCAGCAGGACCTCCTGGAGCCACGGGAGCGACTGGCGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                                                       <  2:311728/135‑1 (MQ=17)
                           cccgcccAGCCGGACCTACAGGAGCGACCGGAGCGACCGGAGCTCCGTGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                                  <  1:430878/135‑1 (MQ=18)
                           ccGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGCGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGTGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                                  <  1:3216350/140‑1 (MQ=255)
                           ccGGACCACCCGGAGCCACAGGAGCGACAGGAGCCACCGGAGCGACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                                                                  <  1:4911876/140‑1 (MQ=18)
                            cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTgggg                                                                                                                 >  2:4060537/1‑140 (MQ=255)
                               ccccgCCCGAGCCACACGAGCGCCCGGAGCCACCCGAGCCGCCGGCGACGCCGGACCAGGAGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                              <  2:4991678/136‑1 (MQ=18)
                               aCCAGCCGGAGGACCAGGACCCACAGGAGCTAAAGGAGCCACCGGGGAAGCAGGACCCGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCGGGGGCCACGGGAGCCACAGGCGCTACGGGGGAAg                                                                                                              <  1:2244804/140‑1 (MQ=18)
                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggggccg                                                                                                              >  2:3316160/1‑137 (MQ=255)
                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                              <  2:1322036/140‑1 (MQ=255)
                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                              <  2:351750/140‑1 (MQ=255)
                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGacg                                                                                                              >  1:5234176/1‑138 (MQ=255)
                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCGGGGCCACGGGAGCCCCAGGGGCTACTGGGGAAGCCGGa                                                                                                         >  2:1491148/1‑140 (MQ=255)
                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                              >  1:2960270/1‑135 (MQ=255)
                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                              <  2:2960270/135‑1 (MQ=255)
                                     cGGAGCTACAGGACCTCAAGGAGCCCAAGGAGCAACCGGCGAACCTGGACGTGCGGGAGGGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACTGGAGCCACAGGCGCTACTGGGGAAGCCGGAc                                                                                                        <  1:5520172/140‑1 (MQ=12)
                                     cGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                                                                                                        <  1:4605292/140‑1 (MQ=255)
                                          ctacaggagcgacaggagcgacaggagcTACCGGGGATGGTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                                   <  1:3699028/138‑1 (MQ=255)
                                          cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                                   <  1:4041040/140‑1 (MQ=255)
                                            ccaggagcgcccggacccccaggcgcTCCCGGAGCAGCCGGACCGCCCGGAGCGACGGGAGCGACAGGAGCACCCGGGCAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                 <  2:2379160/139‑1 (MQ=17)
                                            acaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                 <  1:4904051/140‑1 (MQ=255)
                                             ccggcgcgacaggagcccccggagcTCCCGCGGACGCGGGACCAGCTGGAGGGACGGGAGCGACCGGAGCCCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                <  2:1265829/135‑1 (MQ=18)
                                             caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTCGGGAATCCGGACCAGCCGGa                                                                                                >  1:4801259/1‑140 (MQ=255)
                                                   cgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                          <  1:1651925/140‑1 (MQ=255)
                                                     acaggagcgacaggagcTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGGGCTACCGGGGAAGCCGGACCAGCCGGGGCCACCGGAGCGACAGGGGCTCCCGGGGCAGCCGGACCACCCGGAGCCACAgc                                                                                        >  2:3278553/1‑139 (MQ=18)
                                                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCCACTGGCGCTACAGGCGCAGCCGCACCAGCCGCAGCCACAgc                                                                                        >  1:5275327/1‑139 (MQ=18)
                                                          cgccccaggagcGACCGGGGAACCGGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                   <  1:4084137/135‑1 (MQ=18)
                                                          agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCtc                                                                                   >  1:2862608/1‑139 (MQ=18)
                                                           gcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCtcc                                                                                  >  2:377693/1‑138 (MQ=255)
                                                              actggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                               <  1:4194359/137‑1 (MQ=255)
                                                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACTGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGCCGGGGCCACTGGACCAACACGCCCAACGCGCGAAGCCGCACCTGCCGGAGCCACAGGACCTACCgg                                                                               >  1:5424269/1‑140 (MQ=17)
                                                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACGGGGGACGCCGGACCAGCCGGAGCGACGGGAGCGACAGCGGCTACTCGTCACCCCGGACCCGCGGCACCGACAGGAGCtcgctg                                                                               >  1:845438/1‑135 (MQ=14)
                                                                aggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                             >  2:566100/1‑140 (MQ=255)
                                                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                    <  1:4100916/140‑1 (MQ=255)
                                                                         cGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                    <  1:3578843/140‑1 (MQ=255)
                                                                         aggggccgCTGGACTTGCTTGAGCTAGTGGAGCCAAAGGAGCTACCGGGGTAGCCGGACCCCCCGGTGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                    <  2:3183467/133‑1 (MQ=14)
                                                                           gggAAGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCa                                                                  <  2:1718390/140‑1 (MQ=255)
                                                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                             <  1:5497664/140‑1 (MQ=255)
                                                                                      ccAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                     <  2:4412800/62‑1 (MQ=255)
                                                                                      ccAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                     >  1:4412800/1‑62 (MQ=255)
                                                                                         gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                    <  1:2709703/140‑1 (MQ=255)
                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                   >  1:4847723/1‑140 (MQ=255)
                                                                                           gggAGCGACGGCCGCCCCAGGAGCACCCGGGGCAGCCGGACCCGCCGGCGCCACGGGAGCCACCGGCGCTCCTGGGGTAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                  <  2:4899440/139‑1 (MQ=14)
                                                                                              cgcgcCGGGAGCCCCACGAGCTCCCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                               <  2:4801259/139‑1 (MQ=17)
                                                                                              aGCGACGGGAGCGACAGGCGCTTCCGCGGACCCGGGACCACCCGCTGCCACCGGAGCCCCAGGCGCTTCAGGTCAAGCAGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                               <  2:3691526/140‑1 (MQ=9)
                                                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              >  2:966366/1‑61 (MQ=255)
                                                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              <  1:966366/61‑1 (MQ=255)
                                                                                              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                               <  1:566100/140‑1 (MQ=255)
                                                                                               gCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              <  1:1845180/60‑1 (MQ=255)
                                                                                               gCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                                                              >  2:1845099/1‑60 (MQ=255)
                                                                                                  gccggcgcgaccgGAGCTACCGGGGAAGCCGGACCACCCGGAGCCTCGGGAGCCACAGGGGCTACTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacagg                                           <  1:2635865‑M1/134‑15 (MQ=255)
                                                                                                   cGGGAGCCACAGGAGCCCCCGGCCAAGCCGGACCAGCCGGCGCCACCGGAGCCACAGGGGCTACTGGCGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                          <  1:3064414/140‑1 (MQ=17)
                                                                                                    gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCTCACCAGCCCGAGCGCAGGGAGCGACGGTGGAAGGCTGGCCAGCCGTCGGGTCCGGAGCTAGCgcgg                                         >  2:1034492/1‑140 (MQ=9)
                                                                                                                 gCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                     >  2:1395285/1‑35 (MQ=255)
                                                                                                                 gCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                                                     <  1:2540584/35‑1 (MQ=255)
                                                                                                                  cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGCGCAGCAGGACCAGcc                           >  2:4322662/1‑140 (MQ=17)
                                                                                                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                         >  1:2189976/1‑140 (MQ=255)
                                                                                                                    aCCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCCACAGGCGCTACAGGCGAAGCCGGACCAGCCGGAGCGACACTAGCTACCGGGGAAGCCCGACCAGCCGGAGGGACAGGAGCTCGCGGGGACGCCGGCCCAACCgg                         >  1:2953716/1‑140 (MQ=17)
                                                                                                                     ccGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                        <  2:5783452/140‑1 (MQ=255)
                                                                                                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCCCAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                    <  1:655570/140‑1 (MQ=255)
                                                                                                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                    <  1:17022/140‑1 (MQ=255)
                                                                                                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                    >  1:2549824/1‑140 (MQ=255)
                                                                                                                         ggAAGCCGGACCACCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCTGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                    <  1:348779/140‑1 (MQ=17)
                                                                                                                          gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                                                            <  1:2800701/35‑1 (MQ=255)
                                                                                                                             gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCgcccgg                >  1:3363978/1‑135 (MQ=255)
                                                                                                                             gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGACCgg                >  1:2441686/1‑140 (MQ=17)
                                                                                                                                ggcccACCCGGGGCCACGGGAGCCACAGGGGCAACTGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGc             <  1:5713256/137‑1 (MQ=255)
                                                                                                                                  acactccGGCGCCCCCGGAGCCACAGGCGCTACGGGTGAATCCGGACCTCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa           <  2:3582396/135‑1 (MQ=11)
                                                                                                                                  aCCCGCCGGCGCCCCGGGAGCCACCGGGGCTACGGGCGAAGCCGGACCCGCGGGAGCCACAGGAGCTACCGGGGCCGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa           <  2:3845216/140‑1 (MQ=14)
                                                                                                                                  aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCtc           >  1:1980265/1‑139 (MQ=255)
                                                                                                                                      gCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                           >  2:4174215/1‑56 (MQ=255)
                                                                                                                                      gCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                              <  2:4286164/69‑1 (MQ=255)
                                                                                                                                       ccGGCGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg      <  2:1917128/140‑1 (MQ=255)
                                                                                                                                           ggCCACGGGAGCCCCAGGCGCCACTGGGTAAGCCGGAGCCCCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg  <  2:4263012/140‑1 (MQ=14)
                                                                                                                                           |                                                                                                                                           
CAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607423‑607701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: