Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,638 G→C 13.6% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6380GC13.6% 369.2 / 13.3 182A161P (GCC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (80/68);  new base C (6/21);  total (90/92)
Fisher's exact test for biased strand distribution p-value = 2.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

GCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTA  >  NZ_CP034943/607503‑607773
                                                                                                                                       |                                                                                                                                       
gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                     <  1:5497664/140‑1 (MQ=255)
cccggACCCGCTGGCGGGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                     <  1:5606483/137‑1 (MQ=255)
  tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGTCTGCTGGCGAAGCCTTAGCAGCCGCAGCGTCAGGAGCTACCGGGGACGCAGGACCGGCCGGAg                                                                                                                                   >  1:2712369/1‑140 (MQ=18)
  tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGAg                                                                                                                                   >  2:797095/1‑140 (MQ=255)
   ggACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGc                                                                                                                                  >  1:5783452/1‑140 (MQ=255)
      ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGac                                                                                                                               <  1:4581457‑M1/140‑3 (MQ=255)
          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                           >  1:4847723/1‑140 (MQ=255)
           gggAGCGACGGCCGCCCCAGGAGCACCCGGGGCAGCCGGACCCGCCGGCGCCACGGGAGCCACCGGCGCTCCTGGGGTAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                          <  2:4899440/139‑1 (MQ=14)
              cgcgcCGGGAGCCCCACGAGCTCCCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                       <  2:4801259/139‑1 (MQ=17)
              cgCGACGCGAGCGACATGCGCTACCGGGGAAGCCGGTACAGCCGTTGCCACGGGAGCCACAGCCTATAATGGGGAATCAGTCTCAGCCGTAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                       <  2:2330777/139‑1 (MQ=9)
              aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                       <  1:566100/140‑1 (MQ=255)
                  gccggcgcgaccgGAGCTACCGGGGAAGCCGGACCACCCGGAGCCTCGGGAGCCACAGGGGCTACTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacagg                                                                                                                   <  1:2635865‑M1/134‑15 (MQ=255)
                  aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                                   >  2:3664520/1‑140 (MQ=255)
                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                  >  2:3911406/1‑140 (MQ=255)
                   cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACTGGCGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCGACAggg                                                                                                                  >  2:1417613/1‑140 (MQ=14)
                    gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCgggg                                                                                                                 >  1:1940985/1‑140 (MQ=255)
                               gatcTACCGGGCACGCCGGACCCGCCGGCGCCACTGGAGCCCCCGGCCCTCCTGGCGAACCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGGAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCa                                                                                                      <  1:2955459/137‑1 (MQ=14)
                                 gCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                                    <  2:330622/140‑1 (MQ=255)
                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACCGGAGCTACAGGGGAAGCCGGAGCAGCCgg                                                                                                 >  2:2635865/1‑140 (MQ=17)
                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAACCCGGCCCACCCgg                                                                                                 >  2:1749486/1‑140 (MQ=17)
                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                 >  1:2189976/1‑140 (MQ=255)
                                    aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCAGGACCAACCgg                                                                                                 >  1:2622451/1‑140 (MQ=255)
                                     ccGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                <  2:5783452/140‑1 (MQ=255)
                                         ggcaGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGTCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                            <  1:2685265/137‑1 (MQ=255)
                                         ggaccccGGAGCATCCGGGGCCCCGGGAGCCACAGGCGCTCCCGGCGACCCCGGAGCATCCGGAGCGACCGGAGATCCCGGCCAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGa                                                                                            <  1:3418808/135‑1 (MQ=9)
                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACTGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                            <  1:2807227/140‑1 (MQ=255)
                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                            <  1:17022/140‑1 (MQ=255)
                                         ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                            >  1:2549824/1‑140 (MQ=255)
                                         ggAAGCCGGACCACCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCTGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                            <  1:348779/140‑1 (MQ=17)
                                             gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCgcccgg                                                                                        >  1:3363978/1‑135 (MQ=255)
                                             gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGACCgg                                                                                        >  1:2441686/1‑140 (MQ=17)
                                                  aCCCGCCGGCGCCCCGGGAGCCACCGGGGCTACGGGCGAAGCCGGACCCGCGGGAGCCACAGGAGCTACCGGGGCCGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                                                                   <  2:3845216/140‑1 (MQ=14)
                                                  aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCtc                                                                                   >  1:1980265/1‑139 (MQ=255)
                                                       ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                              <  2:3494240/140‑1 (MQ=255)
                                                       ccGGCGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                              <  2:1917128/140‑1 (MQ=255)
                                                           cgCCACGGGAGCCCCCGGGCCCTCGGGCGCACCCGGACCCGCCGGAGCGACAGGAGCGTCCGGCGAAGCCGGACCAGCCGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                          <  2:4147357/139‑1 (MQ=11)
                                                                 gggAGTCACAGGTGCGCCTGGGCTAGCCGGACCCCCCGGAGCCACAGGAGCTACCGGCGCCGCCGGACCACAAGGAGCGACAGGACCTACCGGTGACGCCGGACCCACCGGAGCCACAGGAGCTACCGGGGAAGCCGGAc                                                                    <  1:3234295/140‑1 (MQ=2)
                                                                 gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCggcc                                                                    >  2:3359320/1‑138 (MQ=14)
                                                                      cccccgcggCTACTGGGGAAGCCGGACCAGCCCGAGCGACAGGACCTACCGGCGAAGCCCGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                               <  1:3990881/133‑1 (MQ=14)
                                                                      ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAGcc                                                               >  1:4742236/1‑140 (MQ=255)
                                                                      ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCACCGTACCAGcc                                                               >  2:5202340/1‑140 (MQ=14)
                                                                      ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGACCGACAGGAGCTCCCGGCCAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGCACCAACCCGAGCGACAGGAGCACCCGCGCACGCCCGACCAGcc                                                               >  2:4329810/1‑140 (MQ=14)
                                                                         caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGATCAACCGGCGACGCCGGAGCACCCGGAGCGAGATGAGAAAACGGCGACGCCAGACCAGcccgc                                                            >  1:4069031/1‑137 (MQ=14)
                                                                           ggggCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGCACACCCGGAGCGACAGTAGCAACCGGGGCCGCCGGGCACGCCGGAGc                                                          >  2:4976359/1‑140 (MQ=14)
                                                                             ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg                                                                                                                                 >  1:5141435/1‑67 (MQ=255)
                                                                             ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                                                                                                 >  1:5421661/1‑67 (MQ=14)
                                                                              gccaCTGGGGAACCCCGACCTGCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                       <  1:4475491/137‑1 (MQ=14)
                                                                               cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                      >  2:1637297/1‑140 (MQ=40)
                                                                                 tcTGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                    <  1:4289773/139‑1 (MQ=255)
                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCAGCCGGACCAACCGGAGCGCCCGGAGCGCCCGGGGCCGCGGGAGCAGCCGGAGCggcgcg                                                    >  2:4294405/1‑135 (MQ=14)
                                                                                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAgg                                                    >  2:3543485/1‑140 (MQ=255)
                                                                                   cggcccaGCCGGAGCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                  <  1:1487599/134‑1 (MQ=255)
                                                                                   aggggAAGCCGGACCCCCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTTCCGGGGAAGCCGGACCACACGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                  <  1:988535/139‑1 (MQ=17)
                                                                                      tgcagagggAGCATCCGGAGCCACAGGAGCTCCCGGGGCAGCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                               <  2:5587565/133‑1 (MQ=14)
                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                             >  2:1996243/1‑140 (MQ=255)
                                                                                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGacg                                                                                                                              >  1:4158443‑M1/1‑54 (MQ=255)
                                                                                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCgg                                           >  2:3914623/1‑140 (MQ=255)
                                                                                          gCCGGACCAGCCGGAGCCACCGGAGCTACCGGTGAACCCGGAGCCCCCGGAGCCACAGGAGCTCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                           <  1:4702689/140‑1 (MQ=14)
                                                                                            cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                         >  2:2385258/1‑140 (MQ=255)
                                                                                            cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                         >  2:3406986/1‑140 (MQ=255)
                                                                                            cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGACCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCGACCgggg                                         >  2:1600216/1‑140 (MQ=255)
                                                                                            cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGACCGACCgggc                                         >  2:184195/1‑139 (MQ=255)
                                                                                               accaccCGGAGCGACCGGAGCTACCGGCGCACCCGGACCAGCCGGAGCCACAGGCGCTACCGGGGCCCCGGGAGGTACCGGAGACACAGGACCACCCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAg                                      <  1:4672350/140‑1 (MQ=9)
                                                                                               aCCAGCCGGACCTCCAGGAGCTACCGGTGACCCGGGAGCTCCCGGAGCCTCAGGAGCTACCGGGGCAGCCGGAGCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                      <  1:4173917/140‑1 (MQ=14)
                                                                                                ccAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGCGAACCCGGAGCGccgggagccacaggggctacgggggaagtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccac                                     <  1:236389‑M1/140‑93 (MQ=255)
                                                                                                 cAGCCGGAGCGGCCGGCGCTACCGGGGATCCCGGCGCACCCGGAGCTacgggagccacaggtgctacgggggacgccggaccagccggagcgacaggagctaccggggaagccggaccaaccggagggacgggagccaca                                    <  1:384522‑M1/140‑94 (MQ=255)
                                                                                                   ccccgagccACAGGAGCCACCGGCGCACCCGGACCACCCGGACCCccgggagccacaggcgctaccggggacgccggaccacccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacagg                                  <  2:206335‑M1/136‑96 (MQ=255)
                                                                                                    ctggAGCCACCGGAGCTACCGGCGAAGCCGGACCATCCGGAGCTACAGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                 <  2:4574971/138‑1 (MQ=11)
                                                                                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACCGGGGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGa                                 >  2:3080601/1‑140 (MQ=14)
                                                                                                         gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAAc                            >  2:5105178/1‑140 (MQ=255)
                                                                                                         gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                            <  2:3222660/140‑1 (MQ=255)
                                                                                                          cgcccggAGCTACCGGCCAAGCCGGACCACCCGGAGCGCCAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                           <  1:3138348/135‑1 (MQ=14)
                                                                                                             cAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactggg                        <  1:1616125‑M1/140‑106 (MQ=255)
                                                                                                              cggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                       <  1:4785855/139‑1 (MQ=255)
                                                                                                              aGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                       >  1:1139311/1‑140 (MQ=255)
                                                                                                              aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCagcagcag                       >  1:4014800/1‑133 (MQ=11)
                                                                                                              aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                       <  1:1637297/140‑1 (MQ=255)
                                                                                                                   cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGGCAGGAGCTACAGGGGAAGCCGGACAAGCCGGAGCGCCAGGAGCGACCGGCGAACCCGGACCAAACGCAGctgcg                  >  2:5249307/1‑136 (MQ=14)
                                                                                                                   cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                  >  2:1076250/1‑140 (MQ=40)
                                                                                                                   cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                  <  1:217727/140‑1 (MQ=40)
                                                                                                                   cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACg                  <  1:5149387/140‑1 (MQ=255)
                                                                                                                    tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACgg                 >  2:4400523/1‑140 (MQ=255)
                                                                                                                     cctggccaaGCCGGAGCAGCCGGAGCGACACGTTCTACCGGGGACCCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                <  2:120854/133‑1 (MQ=11)
                                                                                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                <  2:2549824/140‑1 (MQ=255)
                                                                                                                      ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACgggc               >  1:4209503/1‑139 (MQ=14)
                                                                                                                        ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                              <  2:2746823/75‑1 (MQ=34)
                                                                                                                           caccccGGCCCAGCCGCAGCGACACGCGCTACCGGCGACGCCGCACCTCCCGGAGCGCCAGGCGCTACCGGGGAAGCCGGACCCTCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac          <  1:1034492/136‑1 (MQ=11)
                                                                                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACAGGGGAGGCAGGACCATCCGGAGCTACAGGAGCGACCTGTCAAGCCCGACCAACCGGAGCGACGGGAGCcaca         >  1:1056103/1‑140 (MQ=14)
                                                                                                                              gCCGGACGAGCCGGAGCATCAGGAGCTCCCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg       <  1:3798434/140‑1 (MQ=14)
                                                                                                                              cccGGCCCAGCCGGACCACCCGGAGCTACCGGTGCCCCGGGAGCCACGGGAGCTACCGGCGCACCCGGCCAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAgg       <  1:2140524/139‑1 (MQ=9)
                                                                                                                                ccgACCAGCCGGCGCGCCTGGAGCGACCGGGGACTCCGGACCCACCGGAGCGACCGGACCTACCGGGGCTGCCGGACCCTCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg     <  2:1170227/138‑1 (MQ=14)
                                                                                                                                cGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg     <  1:1600216/140‑1 (MQ=255)
                                                                                                                                cGGACCCCCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg     <  1:1269724/140‑1 (MQ=255)
                                                                                                                                cGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGCCGGGAGCCACAgggc     >  2:5322948/1‑139 (MQ=255)
                                                                                                                                cGGACCAGCCGGAGCGACAGGCGCTTCCGGGGTCGCCGGACCACCCGGAGCGACTGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg     <  2:5486543/140‑1 (MQ=14)
                                                                                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg     <  1:34479/140‑1 (MQ=255)
                                                                                                                                cGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGAGCCACCGGCGCGCCAGGAGCTCCCGGGGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg     <  1:2642495/140‑1 (MQ=14)
                                                                                                                                 ggACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCCACAGGAGCTCCCGGGGAACCCGGAGCAGCCGGAGCTACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGc    <  1:405275/140‑1 (MQ=14)
                                                                                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                                                                   <  1:3250467/59‑1 (MQ=33)
                                                                                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACCGGGGCTa  >  2:906813/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTA  >  NZ_CP034943/607503‑607773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: