BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,694	C→T	10.9%	T179T (ACC→ACT)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,694	0	C	T	10.9%	483.3 / 47.6	216	T179T (ACC→ACT)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (96/88); new base T (20/2); total (122/94)
Fisher's exact test for biased strand distribution p-value = 4.18e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

Only 100 of 221 total aligned reads displayed.

CCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAG  >  NZ_CP034943/607558‑607833

ccGGCGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                                          <  2:1917128/140‑1 (MQ=255)

         cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAACAGCAGCTACCGGATCAACAGGCGAAGCCGGa                                                                                                                                 >  2:664047/1‑140 (MQ=14)

          gggAGTCACAGGTGCGCCTGGGCTAGCCGGACCCCCCGGAGCCACAGGAGCTACCGGCGCCGCCGGACCACAAGGAGCGACAGGACCTACCGGTGACGCCGGACCCACCGGAGCCACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                <  1:3234295/140‑1 (MQ=2)

          gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCggcc                                                                                                                                >  2:3359320/1‑138 (MQ=14)

              gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCacc                                                                                                                            >  1:120854/1‑138 (MQ=255)

               cccccgcggCTACTGGGGAAGCCGGACCAGCCCGAGCGACAGGACCTACCGGCGAAGCCCGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                           <  1:3990881/133‑1 (MQ=14)

               ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGACCGACAGGAGCTCCCGGCCAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGCACCAACCCGAGCGACAGGAGCACCCGCGCACGCCCGACCAGcc                                                                                                                           >  2:4329810/1‑140 (MQ=14)

                    ggggCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGCACACCCGGAGCGACAGTAGCAACCGGGGCCGCCGGGCACGCCGGAGc                                                                                                                      >  2:4976359/1‑140 (MQ=14)

                       gccaCTGGGGAACCCCGACCTGCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                   <  1:4475491/137‑1 (MQ=14)

                       ccTACTGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACGGGCGAAGCCGGAGCACCCGGAGCGACAGGACCTACCGGCGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                   <  1:2144287/139‑1 (MQ=11)

                        cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                                  >  2:1637297/1‑140 (MQ=40)

                          tcTGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                <  1:4289773/139‑1 (MQ=255)

                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACCgg                                                                                                                >  2:410785/1‑140 (MQ=11)

                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGCCCAGCCGGAGCGACAgg                                                                                                                >  2:1597568/1‑140 (MQ=255)

                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAgg                                                                                                                >  2:3543485/1‑140 (MQ=255)

                            tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                              <  1:4060537/140‑1 (MQ=38)

                            cggcccaGCCGGAGCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                              <  1:1487599/134‑1 (MQ=255)

                             ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGc                                                                                                             >  2:5149387/1‑140 (MQ=40)

                               tgcagagggAGCATCCGGAGCCACAGGAGCTCCCGGGGCAGCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                           <  2:5587565/133‑1 (MQ=14)

                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGCAGCGACAGGAGCTCCCGCGGCCCCCGGAGAAGCCGGAGCGCCAGAAGCtcc                                                                                                          >  1:4199417/1‑138 (MQ=11)

                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                          >  2:1730703/1‑140 (MQ=255)

                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCGACCGGGGACGCAGGACCAGCCGGAGCGACCGGAGCTAc                                                                                                          >  2:5356461/1‑140 (MQ=14)

                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCggagcgaccggagcgacc                                                                                                         >  1:1274577/1‑140 (MQ=11)

                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                         >  2:1996243/1‑140 (MQ=255)

                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGCCGGGCCCGCCGGAGCGGCCGGAGCTAcc                                                                                                         >  2:2853450/1‑140 (MQ=11)

                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCGAcc                                                                                                         >  2:4876458/1‑140 (MQ=255)

                                   gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCgg                                                                                                       >  2:3914623/1‑140 (MQ=255)

                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                     >  2:2385258/1‑140 (MQ=255)

                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGACGTACCGGCGAACCAGGACCCGCCGGAGCTACAGCAGCTAGCgggg                                                                                                     >  2:1600246/1‑140 (MQ=14)

                                        accaccCGGAGCGACCGGAGCTACCGGCGCACCCGGACCAGCCGGAGCCACAGGCGCTACCGGGGCCCCGGGAGGTACCGGAGACACAGGACCACCCGGTGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  <  1:4672350/140‑1 (MQ=9)

                                             ctggAGCCACCGGAGCTACCGGCGAAGCCGGACCATCCGGAGCTACAGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                             <  2:4574971/138‑1 (MQ=11)

                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACCCCGGcccca                                                                                         >  2:3344188/1‑138 (MQ=14)

                                                  gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAAc                                                                                        >  2:5105178/1‑140 (MQ=255)

                                                   cgcccggAGCTACCGGCCAAGCCGGACCACCCGGAGCGCCAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                       <  1:3138348/135‑1 (MQ=14)

                                                       cggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                   <  1:4785855/139‑1 (MQ=255)

                                                       aGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                   >  1:1139311/1‑140 (MQ=255)

                                                       aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCagcagcag                                                                                   >  1:4014800/1‑133 (MQ=11)

                                                       aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                   >  2:34479/1‑140 (MQ=255)

                                                       aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                   <  1:1637297/140‑1 (MQ=255)

                                                           gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTACCGGCGAACCCGGACAAACCGGAGCAAc                                                                               >  1:3354838/1‑140 (MQ=14)

                                                            cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                              <  1:5149387/140‑1 (MQ=255)

                                                             tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACgg                                                                             >  2:4400523/1‑140 (MQ=255)

                                                             tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACgg                                                                             >  2:5747039/1‑140 (MQ=255)

                                                              cctggccaaGCCGGAGCAGCCGGAGCGACACGTTCTACCGGGGACCCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                            <  2:120854/133‑1 (MQ=11)

                                                               ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACgggc                                                                           >  1:4209503/1‑139 (MQ=14)

                                                                    gcaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGTCCACCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                      <  1:1955602/138‑1 (MQ=14)

                                                                    gacgCCGGACCCCCCGGAGCTCCAGGTCCTACCGGTGTAGCCGGACACAGAGGAGTGAGAGGACCTACCGGGGACGCCGGACCTTCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                      <  2:4904989/137‑1 (MQ=9)

                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                      <  2:2558054/140‑1 (MQ=255)

                                                                    ccagccggcgcgacaggagctaccggggaagccggcgaacccggagcgccgggagccACAGGGGCTACGGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                      <  1:236389‑M2/83‑1 (MQ=255)

                                                                    caccccGGCCCAGCCGCAGCGACACGCGCTACCGGCGACGCCGCACCTCCCGGAGCGCCAGGCGCTACCGGGGAAGCCGGACCCTCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac                                                                      <  1:1034492/136‑1 (MQ=11)

                                                                       gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                   <  1:3874885/140‑1 (MQ=255)

                                                                       ccccgagccacaggagccaccggcgcacccggaccacccggacccccgggagccACAGGCGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                   <  2:206335‑M2/86‑1 (MQ=255)

                                                                        ccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccACAGGTGCTAAAGGGCAAGTCGGACCAGCCGGAGCGACAGTAGCAAACGGCGGAGCCGGACCAACCGGAGCGACGGGAGCCACAcgg                                                                  >  2:861209‑M2/54‑140 (MQ=255)

                                                                         cgcaccaccCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  2:5234176/137‑1 (MQ=255)

                                                                         ccgACCAGCCGGCGCGCCTGGAGCGACCGGGGACTCCGGACCCACCGGAGCGACCGGACCTACCGGGGCTGCCGGACCCTCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  2:1170227/138‑1 (MQ=14)

                                                                         cGGACCCCCCGGCCCGCCAGGAGCTACCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:1065125/140‑1 (MQ=14)

                                                                         cGGACCAGCCGGAGCGACAGGCGCTTCCGGGGTCGCCGGACCACCCGGAGCGACTGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  2:5486543/140‑1 (MQ=14)

                                                                         cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:1600246/140‑1 (MQ=255)

                                                                         cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 >  2:2733018/1‑140 (MQ=255)

                                                                         cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:34479/140‑1 (MQ=255)

                                                                         cGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGAGCCACCGGCGCGCCAGGAGCTCCCGGGGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:2642495/140‑1 (MQ=14)

                                                                         cGGACCACCCGGAGAGACCGGAGCACCCGGCCAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                 <  1:4733878/140‑1 (MQ=14)

                                                                                  cGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgggg                                                        >  2:5421470/1‑140 (MQ=255)

                                                                                         aCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGAGCAACCGGAGCGACGGGAGCCCCCGGGGCTCCTGGGGACGTCgg                                                 >  2:4831354/1‑140 (MQ=17)

                                                                                           aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                               >  2:5272807/1‑140 (MQ=255)

                                                                                                cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                          <  1:2733018/140‑1 (MQ=255)

                                                                                                 tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  <  1:3745857/83‑1 (MQ=38)

                                                                                                 tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  >  2:3745857/1‑83 (MQ=38)

                                                                                                 tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                  <  1:850239/83‑1 (MQ=38)

                                                                                                  aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCGACAGGGGCGACTGGGGCGGTCGGAGCAGCCgc                                        >  2:2517266/1‑139 (MQ=17)

                                                                                                  aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                         >  1:5634564/1‑123 (MQ=255)

                                                                                                   ccGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGa                                       <  1:4831354/140‑1 (MQ=255)

                                                                                                    cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                      >  1:5061659/1‑140 (MQ=255)

                                                                                                    cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCCACGGGAGCCACAGGGGCTACGGGGGACTGCGGACCAGCCGGAg                                      >  2:3486226/1‑140 (MQ=17)

                                                                                                       ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                   <  1:5747039/140‑1 (MQ=255)

                                                                                                       ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                   <  1:4400523/140‑1 (MQ=255)

                                                                                                        gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCgcc                                  >  2:4024235/1‑138 (MQ=18)

                                                                                                           gCCGGCCCACCCGGAGCGACAGGACCTACCGGGGAACCCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                               <  1:1552400/140‑1 (MQ=21)

                                                                                                           gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                               >  2:1793631/1‑140 (MQ=255)

                                                                                                           gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                               <  1:5034258/140‑1 (MQ=255)

                                                                                                           gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                               >  2:3255334/1‑140 (MQ=255)

                                                                                                            ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                <  1:92524/58‑1 (MQ=35)

                                                                                                             cgcaccaccCGGCGCGACCGGAGCTACCGGGGCAGCCGGACCAGCCGGATCGCCCGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACCGGGGACGTCGGACCAGCCGGAGCGACAGGAg                             <  2:2441686/137‑1 (MQ=18)

                                                                                                             cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCAAACGGGGAAGCCGGCCCAACCGTAGCGCCGGGAGCGACAGGGGGTCCGGGGGCGGTCGGAGCAGCGGAATCGCCAGGAg                             >  2:2998244/1‑140 (MQ=14)

                                                                                                             cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCACCCGCACCGACAGGAGCTACAGCGGAAGCCGGACCAACCGTAGCGACGGGACCCACAGGCGCTACTCGGGACTTCGGACCAGCCGGAGCGACAGGAg                             >  2:2276613/1‑140 (MQ=18)

                                                                                                                    aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                      >  2:472908/1‑140 (MQ=255)

                                                                                                                     ccGGTGCGCCAGGAGCTACCGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACGGGAGCCACCGGCGCGACTGGGGACGTCGGACCACCCGGAGCGACAGGAGATCCCggg                     >  2:3758571/1‑140 (MQ=18)

                                                                                                                     ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                     <  1:1793631/140‑1 (MQ=255)

                                                                                                                         aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                 >  2:4290677/1‑140 (MQ=255)

                                                                                                                         aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGacg                 >  1:4584820/1‑138 (MQ=255)

                                                                                                                         aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCAACCGGAGCGGCGGGAGCCACAGCGGCGACTGGGGACGTCGGACCCGCCGCAGCGACAGGAGCTACGGGGGAAg                 >  1:2577210/1‑140 (MQ=21)

                                                                                                                          gccACAGGGGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                <  1:3640776‑M2/137‑1 (MQ=255)

                                                                                                                          gccACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                         >  2:453286‑M2/4‑35 (MQ=255)

                                                                                                                             accggAGCGACCGGCGCATCCCGACCACCCGGAGCGACAGGAGCTCCCGGGGATCCCGGACCAACCGGATCGACGGGAGCGACAGGGGATTCTGGGGCCGTTGTACCACCAGGAGCGACTGGAGCTACCGGGGAAGCCgg             <  1:5387497/137‑1 (MQ=12)

                                                                                                                             aCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg             <  1:2189461/140‑1 (MQ=255)

                                                                                                                              cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa            >  1:4050548/1‑140 (MQ=255)

                                                                                                                                   gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc       >  2:4251833/1‑140 (MQ=255)

                                                                                                                                   gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc       <  1:5105178/140‑1 (MQ=255)

                                                                                                                                     tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCg     >  1:2104473/1‑140 (MQ=17)

                                                                                                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACAGGGTACGTCGGACCTGCCGGATCGAAAGCAGCTACCGGGGAAGCCGGaacaacaca    >  2:2820170/1‑137 (MQ=18)

CCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAG  >  NZ_CP034943/607558‑607833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`