BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	20.4%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	20.4%	341.2 / 78.9	230	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (94/82); new base C (39/11); total (135/95)
Fisher's exact test for biased strand distribution p-value = 1.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 233 total aligned reads displayed.

GGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACA  >  NZ_CP034943/607562‑607838

     cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAACAGCAGCTACCGGATCAACAGGCGAAGCCGGa                                                                                                                                      >  2:664047/1‑140 (MQ=14)

      gggAGTCACAGGTGCGCCTGGGCTAGCCGGACCCCCCGGAGCCACAGGAGCTACCGGCGCCGCCGGACCACAAGGAGCGACAGGACCTACCGGTGACGCCGGACCCACCGGAGCCACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                     <  1:3234295/140‑1 (MQ=2)

      gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCggcc                                                                                                                                     >  2:3359320/1‑138 (MQ=14)

          gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCacc                                                                                                                                 >  1:120854/1‑138 (MQ=255)

           cccccgcggCTACTGGGGAAGCCGGACCAGCCCGAGCGACAGGACCTACCGGCGAAGCCCGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                <  1:3990881/133‑1 (MQ=14)

           ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAGcc                                                                                                                                >  1:4742236/1‑140 (MQ=255)

           ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGATGCCGGAGCAGcc                                                                                                                                >  1:206335/1‑140 (MQ=255)

           ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGACCGACAGGAGCTCCCGGCCAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACGCCGCACCAACCCGAGCGACAGGAGCACCCGCGCACGCCCGACCAGcc                                                                                                                                >  2:4329810/1‑140 (MQ=14)

              caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGATCAACCGGCGACGCCGGAGCACCCGGAGCGAGATGAGAAAACGGCGACGCCAGACCAGcccgc                                                                                                                             >  1:4069031/1‑137 (MQ=14)

                   ccTACTGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACGGGCGAAGCCGGAGCACCCGGAGCGACAGGACCTACCGGCGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                        <  1:2144287/139‑1 (MQ=11)

                        aggggAAGCCGGACCCCCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTTCCGGGGAAGCCGGACCACACGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                   <  1:988535/139‑1 (MQ=17)

                           tgcagagggAGCATCCGGAGCCACAGGAGCTCCCGGGGCAGCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                <  2:5587565/133‑1 (MQ=14)

                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGCAGCGACAGGAGCTCCCGCGGCCCCCGGAGAAGCCGGAGCGCCAGAAGCtcc                                                                                                               >  1:4199417/1‑138 (MQ=11)

                             aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCggagcgaccggagcgacc                                                                                                              >  1:1274577/1‑140 (MQ=11)

                             aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                              >  2:1996243/1‑140 (MQ=255)

                             aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                              >  1:448771/1‑140 (MQ=255)

                               gCCGGACCAGCCGGAGCCACCGGAGCTACCGGTGAACCCGGAGCCCCCGGAGCCACAGGAGCTCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                            <  1:4702689/140‑1 (MQ=14)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                          >  2:2385258/1‑140 (MQ=255)

                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGACCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCGACCgggg                                                                                                          >  2:1600216/1‑140 (MQ=255)

                                         ctggAGCCACCGGAGCTACCGGCGAAGCCGGACCATCCGGAGCTACAGGAGCTACCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  2:4574971/138‑1 (MQ=11)

                                              gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                             <  2:3222660/140‑1 (MQ=255)

                                                   aGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                        >  1:1139311/1‑140 (MQ=255)

                                                   aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCagcagcag                                                                                        >  1:4014800/1‑133 (MQ=11)

                                                   aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                        >  2:34479/1‑140 (MQ=255)

                                                   aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                        <  1:1637297/140‑1 (MQ=255)

                                                        cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                   <  1:5149387/140‑1 (MQ=255)

                                                         tACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACgg                                                                                  >  2:4400523/1‑140 (MQ=255)

                                                          cctggccaaGCCGGAGCAGCCGGAGCGACACGTTCTACCGGGGACCCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 <  2:120854/133‑1 (MQ=11)

                                                           ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACgggc                                                                                >  1:4209503/1‑139 (MQ=14)

                                                                gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  2:2558054/140‑1 (MQ=255)

                                                                ccagccggcgcgacaggagctaccggggaagccggcgaacccggagcgccgggagccACAGGGGCTACGGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  1:236389‑M2/83‑1 (MQ=255)

                                                                caccccGGCCCAGCCGCAGCGACACGCGCTACCGGCGACGCCGCACCTCCCGGAGCGCCAGGCGCTACCGGGGAAGCCGGACCCTCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac                                                                           <  1:1034492/136‑1 (MQ=11)

                                                                 cagccggagcggccggcgctaccggggatcccggcgcacccggagctacgggagccACAGGTGCTACGGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGGGACGGGAGCcaca                                                                          <  1:384522‑M2/84‑1 (MQ=255)

                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACAGGGGAGGCAGGACCATCCGGAGCTACAGGAGCGACCTGTCAAGCCCGACCAACCGGAGCGACGGGAGCcaca                                                                          >  1:1056103/1‑140 (MQ=14)

                                                                   gCCGGACCCCCCGGCCCCACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        <  1:447069/140‑1 (MQ=14)

                                                                   ccccgagccacaggagccaccggcgcacccggaccacccggacccccgggagccACAGGCGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        <  2:206335‑M2/86‑1 (MQ=255)

                                                                    ccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccACAGGTGCTAAAGGGCAAGTCGGACCAGCCGGAGCGACAGTAGCAAACGGCGGAGCCGGACCAACCGGAGCGACGGGAGCCACAcgg                                                                       >  2:861209‑M2/54‑140 (MQ=255)

                                                                     cgcaccaccCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      <  2:5234176/137‑1 (MQ=255)

                                                                     ccgACCAGCCGGCGCGCCTGGAGCGACCGGGGACTCCGGACCCACCGGAGCGACCGGACCTACCGGGGCTGCCGGACCCTCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      <  2:1170227/138‑1 (MQ=14)

                                                                     cGGACCCCCCGGCCCGCCAGGAGCTACCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      <  1:1065125/140‑1 (MQ=14)

                                                                     cGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGCCGGGAGCCACAgggc                                                                      >  2:5322948/1‑139 (MQ=255)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      <  1:1600246/140‑1 (MQ=255)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      >  2:2733018/1‑140 (MQ=255)

                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      <  1:3406986/140‑1 (MQ=255)

                                                                     cGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGAGCCACCGGCGCGCCAGGAGCTCCCGGGGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      <  1:2642495/140‑1 (MQ=14)

                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGAAACCGGAGAGACACGAGCTACCGGGGAACCCGGAGAAGCCGGAGAGGCAGGAGCTACAGGGGAACCCGGTGCAACCGGAGCGACGGGAGCGACAGGGGCTa                                                                   >  2:5500378/1‑140 (MQ=14)

                                                                             ccGGAGCGACAGGCGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                              <  1:3316160/140‑1 (MQ=255)

                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCTACAGGGGAATCCGGACCAGCCGGACCAACAGGAGAGACCGGGGCAGCCGGACCAAGCGGAGCGCCGGGGGCGAGAGGGGCGACTGGGGCCg                                                          >  1:1916292/1‑140 (MQ=14)

                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                          >  2:1616125/1‑140 (MQ=255)

                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                          >  2:1738743/1‑140 (MQ=255)

                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                          <  1:5051264/140‑1 (MQ=255)

                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCTACAGGAGCTACAGGGGAACCCGGACCAACCGGAGCTACGGGAGCCACAGGGGCTACTGGGTAca                                                          >  2:329425/1‑139 (MQ=17)

                                                                                     aCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGAGCAACCGGAGCGACGGGAGCCCCCGGGGCTCCTGGGGACGTCgg                                                      >  2:4831354/1‑140 (MQ=17)

                                                                                           gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGc                                                >  2:4961687/1‑140 (MQ=255)

                                                                                            cTACCGGGGAAGCCGGCCCAACCGGCGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACGGGGGACGTCGGACCAGcc                                               <  1:5322948/140‑1 (MQ=18)

                                                                                            cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                               <  1:2733018/140‑1 (MQ=255)

                                                                                             tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:3745857/83‑1 (MQ=38)

                                                                                             tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:850239/83‑1 (MQ=38)

                                                                                             tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       >  2:3745857/1‑83 (MQ=38)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCGACAGGGGCGACTGGGGCGGTCGGAGCAGCCgc                                             >  2:2517266/1‑139 (MQ=17)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                              >  1:5634564/1‑123 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                              <  2:5634564/123‑1 (MQ=255)

                                                                                               ccGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGa                                            <  1:4831354/140‑1 (MQ=255)

                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCCACGGGAGCCACAGGGGCTACGGGGGACTGCGGACCAGCCGGAg                                           >  2:3486226/1‑140 (MQ=17)

                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACGGGGGACGTCGGACCAGCCGGAGCGa                                        >  2:2267552/1‑140 (MQ=255)

                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGCAGCCGGACCTACCGGAGCGACGGGCGCCCCGGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGa                                        >  1:4334039/1‑140 (MQ=18)

                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                        <  1:5747039/140‑1 (MQ=255)

                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCgcc                                       >  2:4024235/1‑138 (MQ=18)

                                                                                                       gCCGGCCCACCCGGAGCGACAGGACCTACCGGGGAACCCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    <  1:1552400/140‑1 (MQ=21)

                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    >  2:1793631/1‑140 (MQ=255)

                                                                                                       cccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    <  1:5014178/139‑1 (MQ=255)

                                                                                                         cgcaccaccCGGCGCGACCGGAGCTACCGGGGCAGCCGGACCAGCCGGATCGCCCGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACAGGGGCTACCGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                  <  2:2441686/137‑1 (MQ=18)

                                                                                                         cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCAAACGGGGAAGCCGGCCCAACCGTAGCGCCGGGAGCGACAGGGGGTCCGGGGGCGGTCGGAGCAGCGGAATCGCCAGGAg                                  >  2:2998244/1‑140 (MQ=14)

                                                                                                         cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAGGAg                                  >  2:4044266/1‑140 (MQ=255)

                                                                                                            aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTCCGCGGGACGACGGACCAGCCGGATCGACAGGAGcac                               >  2:2834117/1‑138 (MQ=18)

                                                                                                                 ccGGTGCGCCAGGAGCTACCGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACGGGAGCCACCGGCGCGACTGGGGACGTCGGACCACCCGGAGCGACAGGAGATCCCggg                          >  2:3758571/1‑140 (MQ=18)

                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                          <  1:1793631/140‑1 (MQ=255)

                                                                                                                     aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                      >  2:4290677/1‑140 (MQ=255)

                                                                                                                     aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCAACCGGAGCGGCGGGAGCCACAGCGGCGACTGGGGACGTCGGACCCGCCGCAGCGACAGGAGCTACGGGGGAAg                      >  1:2577210/1‑140 (MQ=21)

                                                                                                                      gccACAGGGGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                     <  1:3640776‑M2/137‑1 (MQ=255)

                                                                                                                      gccACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                              >  2:453286‑M2/4‑35 (MQ=255)

                                                                                                                         aCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                  <  1:2189461/140‑1 (MQ=255)

                                                                                                                          cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 >  1:4050548/1‑140 (MQ=255)

                                                                                                                           aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                >  2:5181513/1‑140 (MQ=255)

                                                                                                                            ggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc               >  1:4615404/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAAc            >  2:5117881/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  2:490281/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  2:3582197/1‑140 (MQ=255)

                                                                                                                               gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  2:4251833/1‑140 (MQ=255)

                                                                                                                               gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            <  1:5105178/140‑1 (MQ=255)

                                                                                                                                cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc           >  2:5416081/1‑140 (MQ=255)

                                                                                                                                  actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCTACGGGAGCCACAGGCGCTACTGGGGACGGCGGACCAGCCGGAGCCGCAGGAGCTACCGGGGACGCCGGAACAGCCgc         >  2:1703008/4‑139 (MQ=14)

                                                                                                                                    cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAg       >  1:1383535/1‑140 (MQ=255)

                                                                                                                                     ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGc      >  2:5750208/1‑140 (MQ=255)

                                                                                                                                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa    >  2:1640733/1‑140 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                          >  2:4986737/1‑69 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   >  2:4839808/1‑140 (MQ=255)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGAcg  >  2:4778809/1‑139 (MQ=255)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGAcg  >  2:3984272/1‑139 (MQ=255)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcg  >  2:5428203/1‑139 (MQ=255)

GGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACA  >  NZ_CP034943/607562‑607838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`