BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,702	C→T	12.5%	A182V (GCC→GTC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,702	0	C	T	12.5%	547.5 / 67.3	240	A182V (GCC→GTC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (114/96); new base T (26/4); total (140/100)
Fisher's exact test for biased strand distribution p-value = 6.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607565‑607840

  cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCAGGACCAACAGCAGCTACCGGATCAACAGGCGAAGCCGGa                                                                                                                                        >  2:664047/1‑140 (MQ=14)

   gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCggcc                                                                                                                                       >  2:3359320/1‑138 (MQ=14)

       gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCacc                                                                                                                                   >  1:120854/1‑138 (MQ=255)

        ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAGcc                                                                                                                                  >  1:4742236/1‑140 (MQ=255)

        ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGATGCCGGAGCAGcc                                                                                                                                  >  1:206335/1‑140 (MQ=255)

             ggggCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGCACACCCGGAGCGACAGTAGCAACCGGGGCCGCCGGGCACGCCGGAGc                                                                                                                             >  2:4976359/1‑140 (MQ=14)

                gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAATCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                          <  1:1536350/140‑1 (MQ=255)

                ccTACTGGGGAAGCCGGACCAGCCGGAGCCACCGGAGCGACGGGCGAAGCCGGAGCACCCGGAGCGACAGGACCTACCGGCGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                          <  1:2144287/139‑1 (MQ=11)

                   tcTGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:4289773/139‑1 (MQ=255)

                   aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCAGCCGGACCAACCGGAGCGCCCGGAGCGCCCGGGGCCGCGGGAGCAGCCGGAGCggcgcg                                                                                                                       >  2:4294405/1‑135 (MQ=14)

                     tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                     <  1:4060537/140‑1 (MQ=38)

                      ggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGc                                                                                                                    >  2:5149387/1‑140 (MQ=40)

                        tgcagagggAGCATCCGGAGCCACAGGAGCTCCCGGGGCAGCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  <  2:5587565/133‑1 (MQ=14)

                         gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCGACCGGGGACGCAGGACCAGCCGGAGCGACCGGAGCTAc                                                                                                                 >  2:5356461/1‑140 (MQ=14)

                          aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                                >  2:1996243/1‑140 (MQ=255)

                          aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGACGCCGGGCCCGCCGGAGCGGCCGGAGCTAcc                                                                                                                >  2:2853450/1‑140 (MQ=11)

                          aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                                >  1:448771/1‑140 (MQ=255)

                          aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCGAcc                                                                                                                >  2:4876458/1‑140 (MQ=255)

                            gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCgg                                                                                                              >  2:3914623/1‑140 (MQ=255)

                            gCCGGACCAGCCGGAGCCACCGGAGCTACCGGTGAACCCGGAGCCCCCGGAGCCACAGGAGCTCCCGGGGAAGCCGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                              <  1:4702689/140‑1 (MQ=14)

                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                            >  2:2385258/1‑140 (MQ=255)

                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGACCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCGACCgggg                                                                                                            >  2:1600216/1‑140 (MQ=255)

                              cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAAAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGACCGACCgggc                                                                                                            >  2:184195/1‑139 (MQ=255)

                                 aCCAGCCGGACCTCCAGGAGCTACCGGTGACCCGGGAGCTCCCGGAGCCTCAGGAGCTACCGGGGCAGCCGGAGCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:4173917/140‑1 (MQ=14)

                                      ccGGAGCGACAGGAGCCCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  1:4028264/140‑1 (MQ=255)

                                           gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAAc                                                                                               >  2:5105178/1‑140 (MQ=255)

                                           gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                               <  2:3222660/140‑1 (MQ=255)

                                                aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                          >  2:34479/1‑140 (MQ=255)

                                                aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                          <  1:1637297/140‑1 (MQ=255)

                                                     cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                     <  1:5149387/140‑1 (MQ=255)

                                                       cctggccaaGCCGGAGCAGCCGGAGCGACACGTTCTACCGGGGACCCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   <  2:120854/133‑1 (MQ=11)

                                                       aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   <  2:2549824/140‑1 (MQ=255)

                                                        ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACgggc                                                                                  >  1:4209503/1‑139 (MQ=14)

                                                             gcaGCCGGACCTCCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAAGCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:4330319/138‑1 (MQ=14)

                                                             gacgCCGGACCCCCCGGAGCTCCAGGTCCTACCGGTGTAGCCGGACACAGAGGAGTGAGAGGACCTACCGGGGACGCCGGACCTTCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:4904989/137‑1 (MQ=9)

                                                             gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:2558054/140‑1 (MQ=255)

                                                             caccccGGCCCAGCCGCAGCGACACGCGCTACCGGCGACGCCGCACCTCCCGGAGCGCCAGGCGCTACCGGGGAAGCCGGACCCTCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGCGCGACGGGAGCcac                                                                             <  1:1034492/136‑1 (MQ=11)

                                                              caGCCGGACCACCCGGACCCCCAGGAGCAACCGGTGACCCCGGACCCACCGGAGCCACAGGCGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                            <  1:3463545/139‑1 (MQ=14)

                                                              aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACAGGGGAGGCAGGACCATCCGGAGCTACAGGAGCGACCTGTCAAGCCCGACCAACCGGAGCGACGGGAGCcaca                                                                            >  1:1056103/1‑140 (MQ=14)

                                                                gCCGGACGAGCCGGAGCATCAGGAGCTCCCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  1:3798434/140‑1 (MQ=14)

                                                                gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGCGCGGAGCCACCGGGGACGCCGGACCAACCGGAGCGCCGCGAGcgaacgg                                                                          >  2:3499745/1‑134 (MQ=14)

                                                                ccccgagccacaggagccaccggcgcacccggaccacccggacccccgggagccACAGGCGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  2:206335‑M2/86‑1 (MQ=255)

                                                                 ccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccACAGGTGCTAAAGGGCAAGTCGGACCAGCCGGAGCGACAGTAGCAAACGGCGGAGCCGGACCAACCGGAGCGACGGGAGCCACAcgg                                                                         >  2:861209‑M2/54‑140 (MQ=255)

                                                                  cgcaccaccCGGAGCTACACGAGCCCCAGGCGAACCCGGACCAAGCGGTGCGACCGGGGCTCCGGGAGCTGCCGCACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  1:399520/137‑1 (MQ=9)

                                                                  cgcaccaccCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  2:5234176/137‑1 (MQ=255)

                                                                  ccgACCAGCCGGCGCGCCTGGAGCGACCGGGGACTCCGGACCCACCGGAGCGACCGGACCTACCGGGGCTGCCGGACCCTCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  2:1170227/138‑1 (MQ=14)

                                                                  cGGACCCCCCGGCCCGCCAGGAGCTACCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  1:1065125/140‑1 (MQ=14)

                                                                  cGGACCAGCCGGAGCGACAGGCGCTTCCGGGGTCGCCGGACCACCCGGAGCGACTGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  2:5486543/140‑1 (MQ=14)

                                                                  cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  1:2385258/140‑1 (MQ=255)

                                                                  cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  1:1600246/140‑1 (MQ=255)

                                                                  cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  1:3406986/140‑1 (MQ=255)

                                                                  cGGACCAGCCGGAGCCACCGGAGCTACCGGCGAAGCCGGAGCCACCGGCGCGCCAGGAGCTCCCGGGGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                        <  1:2642495/140‑1 (MQ=14)

                                                                          ccGGAGCGACAGGCGCGACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                                <  1:3316160/140‑1 (MQ=255)

                                                                              agctaccggggaagctggaccagctggagcgacgggagcgACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            <  1:4829079‑M2/100‑1 (MQ=255)

                                                                              aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCTACAGGGGAATCCGGACCAGCCGGACCAACAGGAGAGACCGGGGCAGCCGGACCAAGCGGAGCGCCGGGGGCGAGAGGGGCGACTGGGGCCg                                                            >  1:1916292/1‑140 (MQ=14)

                                                                              aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            >  2:1616125/1‑140 (MQ=255)

                                                                              aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            >  2:1738743/1‑140 (MQ=255)

                                                                              aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCTACAGGAGCTACAGGGGAACCCGGACCAACCGGAGCTACGGGAGCCACAGGGGCTACTGGGTAca                                                            >  2:329425/1‑139 (MQ=17)

                                                                              aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGAGCGGGGCCCCCGGACCAACCGGAGCGACGGGAGCGACAGGGGCGACGGGGGACg                                                            >  2:4450349/1‑140 (MQ=14)

                                                                                  aCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCgg                                                        >  2:4832928/1‑140 (MQ=255)

                                                                                    aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                      >  2:5272807/1‑140 (MQ=255)

                                                                                          tACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:850239/83‑1 (MQ=38)

                                                                                           aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                                >  1:5634564/1‑123 (MQ=255)

                                                                                           aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                                <  2:5634564/123‑1 (MQ=255)

                                                                                             cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGGCGCCGGACCACCCGGAGCGCCGGGAGCCACCGCGGGTACGGGGGACGTCTGACCAGCCGGAg                                             >  1:321676/1‑140 (MQ=17)

                                                                                             cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                             >  1:5061659/1‑140 (MQ=255)

                                                                                                ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                          <  1:4400523/140‑1 (MQ=255)

                                                                                                ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                          >  1:3944844/1‑140 (MQ=255)

                                                                                                 gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCgcc                                         >  2:4024235/1‑138 (MQ=18)

                                                                                                    cccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      <  1:5014178/139‑1 (MQ=255)

                                                                                                     ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:92524/58‑1 (MQ=35)

                                                                                                      cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCAAACGGGGAAGCCGGCCCAACCGTAGCGCCGGGAGCGACAGGGGGTCCGGGGGCGGTCGGAGCAGCGGAATCGCCAGGAg                                    >  2:2998244/1‑140 (MQ=14)

                                                                                                      cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAGGAg                                    >  2:4044266/1‑140 (MQ=255)

                                                                                                      cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCACCCGCACCGACAGGAGCTACAGCGGAAGCCGGACCAACCGTAGCGACGGGACCCACAGGCGCTACTCGGGACTTCGGACCAGCCGGAGCGACAGGAg                                    >  2:2276613/1‑140 (MQ=18)

                                                                                                         aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                 >  2:2470651/1‑140 (MQ=255)

                                                                                                              ccGGTGCGCCAGGAGCTACCGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACGGGAGCCACCGGCGCGACTGGGGACGTCGGACCACCCGGAGCGACAGGAGATCCCggg                            >  2:3758571/1‑140 (MQ=18)

                                                                                                              ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:1793631/140‑1 (MQ=255)

                                                                                                                  aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGacg                        >  1:4584820/1‑138 (MQ=255)

                                                                                                                  aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCAACCGGAGCGGCGGGAGCCACAGCGGCGACTGGGGACGTCGGACCCGCCGCAGCGACAGGAGCTACGGGGGAAg                        >  1:2577210/1‑140 (MQ=21)

                                                                                                                      accggAGCGACCGGCGCATCCCGACCACCCGGAGCGACAGGAGCTCCCGGGGATCCCGGACCAACCGGATCGACGGGAGCGACAGGGGATTCTGGGGCCGTTGTACCACCAGGAGCGACTGGAGCTACCGGGGAAGCCgg                    <  1:5387497/137‑1 (MQ=12)

                                                                                                                      aCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                    <  1:2189461/140‑1 (MQ=255)

                                                                                                                        aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                  >  2:4719821/1‑140 (MQ=255)

                                                                                                                            gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              >  2:3582197/1‑140 (MQ=255)

                                                                                                                            gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              <  1:5105178/140‑1 (MQ=255)

                                                                                                                              tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGCCGTCGGACCAGCCGGATCGACAGCAGCTACGGGGGAAGCCGGACCAAccc            >  2:4801460/1‑139 (MQ=17)

                                                                                                                               actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCTACGGGAGCCACAGGCGCTACTGGGGACGGCGGACCAGCCGGAGCCGCAGGAGCTACCGGGGACGCCGGAACAGCCgc           >  2:1703008/4‑139 (MQ=14)

                                                                                                                               actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg           >  1:1159769/4‑140 (MQ=255)

                                                                                                                               aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACAGGGTACGTCGGACCTGCCGGATCGAAAGCAGCTACCGGGGAAGCCGGaacaacaca           >  2:2820170/1‑137 (MQ=18)

                                                                                                                                ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                     <  2:4537338/65‑1 (MQ=31)

                                                                                                                                ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa          >  2:5749106/1‑140 (MQ=255)

                                                                                                                                 tggcgccgCCGGACCCCCCGGAGCCACCGGAGCTACCGGGGCAGCCGGACCACCCGGAGCGACTGGAGCCACAGGGGCTACTGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg         <  1:1338616/133‑1 (MQ=14)

                                                                                                                                 cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAg         >  1:1383535/1‑140 (MQ=255)

                                                                                                                                    ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa      >  2:1640733/1‑140 (MQ=255)

                                                                                                                                      aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcg    >  2:5428203/1‑139 (MQ=255)

                                                                                                                                        gCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                                                     <  2:4158443/57‑1 (MQ=9)

                                                                                                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                                 <  2:3738416/77‑1 (MQ=255)

                                                                                                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                                 >  1:3738416/1‑77 (MQ=255)

                                                                                                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg  <  1:2912445/140‑1 (MQ=255)

                                                                                                                                        gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg  <  1:4961687/140‑1 (MQ=255)

                                                                                                                                        gCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg  <  1:797095/140‑1 (MQ=255)

CACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607565‑607840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`