Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 686,638 C→G 10.0% L103V (CTT→GTT)  purM → phosphoribosylformylglycinamidine cyclo‑ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943686,6380CG10.0% 1250.0 / 20.1 471L103V (CTT→GTT) purMphosphoribosylformylglycinamidine cyclo‑ligase
Reads supporting (aligned to +/- strand):  ref base C (93/315);  new base G (45/2);  total (153/318)
Fisher's exact test for biased strand distribution p-value = 9.10e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 472 total aligned reads displayed.

TTAATTTCGGGAACGGACGGTGTCGGCACCAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGATTATATACAGCAG  >  NZ_CP034943/686500‑686776
                                                                                                                                          |                                                                                                                                          
 tAATTTCGGGAACGGACGGTGTCGGCACCAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCtt                                                                                                                                          <  1:1934582/140‑1 (MQ=255)
                    tgtCGGCACCAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTTATTATTTAGCGGTCGGaa                                                                                                                       >  2:732704/1‑140 (MQ=255)
                     gtCGGCACCAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGaaa                                                                                                                      >  2:103047/1‑140 (MQ=255)
                       cGGCACCAAATTAAAGCTCGCTTGTTCCATGGCTAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAg                                                                                                                    <  1:5674072/140‑1 (MQ=255)
                            ccAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGAc                                                                                                               <  2:2830947/140‑1 (MQ=255)
                            ccAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGAc                                                                                                               <  2:2973975/140‑1 (MQ=255)
                            ccAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGAc                                                                                                               <  1:5331567/140‑1 (MQ=255)
                            acAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGAc                                                                                                               <  2:3979949/139‑1 (MQ=255)
                              aaaTTAAAGCTCGCTTTTTCCATTGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCAGTTGTTTTTTATTGTTTATTTAACGTTGCGAAAAGCCGAacc                                                                                                             >  1:4041511/1‑140 (MQ=255)
                              aaaTTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTGGTTTTTTGTTGATTATTTAGCGGTCGGAAAAGCGGAccc                                                                                                             >  1:4314404/1‑140 (MQ=255)
                                 ttAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTAt                                                                                                          <  1:1553965/140‑1 (MQ=255)
                                   aaaGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGa                                                                                                        <  1:5035974/140‑1 (MQ=255)
                                      gCTCGCTTGTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGaaaa                                                                                                     <  2:4777150/140‑1 (MQ=255)
                                         cGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTg                                                                                                  >  2:806579/1‑140 (MQ=255)
                                         cGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTg                                                                                                  >  2:4583410/1‑140 (MQ=255)
                                          gCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTGTTGTTTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAAATGa                                                                                                 >  1:4260762/1‑140 (MQ=255)
                                          gCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTGTTGATTATTTATGGGTCGGAAAACCGGACCCGATGAAAATTGa                                                                                                 >  1:1767764/1‑140 (MQ=255)
                                          gCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCGTGATTATTTAGCGGTCGGAAAAGCGGACCCTATTAAAATTGa                                                                                                 >  2:449435/1‑140 (MQ=255)
                                          gCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTGGTTTTTTGGTGATTATTTATCGGTCGGAAAAGCGCGCCCTAAGaaaaatta                                                                                                 >  1:893516/1‑136 (MQ=255)
                                           cTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAg                                                                                                <  2:1872878/140‑1 (MQ=255)
                                            tttttGCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGATCAGTTGTTTTTTTTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGc                                                                                               <  2:1413373/140‑1 (MQ=255)
                                              tttCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGTTGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAg                                                                                             <  2:1888401/140‑1 (MQ=255)
                                              tttCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAg                                                                                             <  2:2042961/140‑1 (MQ=255)
                                              tttCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAg                                                                                             <  2:2505762/140‑1 (MQ=255)
                                               ttCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGa                                                                                            <  2:3379529/140‑1 (MQ=255)
                                                      gATAAGCATGACACGTTTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  2:5362320/140‑1 (MQ=255)
                                                      gATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGTGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  1:2099695/140‑1 (MQ=255)
                                                      gATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  1:5415256/140‑1 (MQ=255)
                                                      gATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  1:5546793/140‑1 (MQ=255)
                                                      gATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  2:4323159/140‑1 (MQ=255)
                                                      gATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCAATGAAAATTTAGCAGATCGTaaa                                                                                     >  2:775319/1‑138 (MQ=255)
                                                      gATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGAGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  2:5799131/140‑1 (MQ=255)
                                                        tAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAg                                                                                   <  2:2151156/140‑1 (MQ=255)
                                                           gCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGgtg                                                                                <  1:924672/140‑1 (MQ=255)
                                                             aTGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTGTTGGTTATTTATCGGTCGGTAAATCGGGCCCACTGAAAATTGATCAGAAAGTACAAGGTGTa                                                                              >  1:521301/1‑140 (MQ=255)
                                                                acacGCTTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAATGGGACCGGATGAACAGTGGGCAGCTCGTACAATGTGTAGCg                                                                           >  2:4416507/1‑140 (MQ=255)
                                                                acacGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTTTTTTTTATTTATCGGTGGGGAAAGCGGACCCGATGAAAATTTAGCAGATAGTAAAAGGTGTATCg                                                                           >  1:2361241/1‑140 (MQ=255)
                                                                acacGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTGGTGATTATTTAGCGGTCGGAAAAGCGGGCCCGATGAAAATTGAGCAGATCGTACAAGGGGTAGCg                                                                           >  1:3023590/1‑140 (MQ=255)
                                                                 cacGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTTTTTTTTGTTTATTATTTATCGGTCGGTAAATCGGACCCAATGAAAAATTGTCAGATCGTACAAGGTGTTGCGa                                                                          >  1:5597382/1‑140 (MQ=255)
                                                                  acGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTTTAGCGaa                                                                         <  1:1130809/140‑1 (MQ=255)
                                                                  acGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGaa                                                                         <  1:3263109/140‑1 (MQ=255)
                                                                  acGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGaa                                                                         <  2:1660793/140‑1 (MQ=255)
                                                                  acGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGaa                                                                         <  1:4120352/140‑1 (MQ=255)
                                                                     aTTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  1:204546/125‑1 (MQ=255)
                                                                     aTTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACa                                                                                     <  1:1251711/125‑1 (MQ=255)
                                                                      ttGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTGTTGTTTATTTTGCGGTCGGGAAAGCGGAACCCATGAAAATTTCGCAGATCGGACAAGGTGTTGCGAACCGc                                                                     >  1:5791841/1‑140 (MQ=255)
                                                                      ttGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCt                                                                                                                                           >  2:3881335/1‑70 (MQ=255)
                                                                      ttGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGTATATTTAGCGGTCGGAAAAACGGACCCTATGAAAATTGAGCAGATAGTACAAGGGGTAGCGAACGGc                                                                     >  2:3293832/1‑140 (MQ=255)
                                                                      ttGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTAc                                                                                      <  1:4211459/123‑1 (MQ=255)
                                                                      ttGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTAc                                                                                      >  2:4211459/1‑123 (MQ=255)
                                                                            ttGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTATCGGTCGGAAAAGCGGACCCTATGAAAAATTAGCAGATAGTAAAAGGTGTAGCGAAAGGCTGTGAg                                                               >  2:3147477/1‑140 (MQ=255)
                                                                            ttGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAg                                                               <  2:514990/140‑1 (MQ=255)
                                                                            ttGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAg                                                               <  2:4692762/140‑1 (MQ=255)
                                                                            ttGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAg                                                               <  1:1876670/140‑1 (MQ=255)
                                                                              gACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCa                                                             >  2:2666132/1‑140 (MQ=255)
                                                                              gACGCTGTGGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTGGTTTTTTCGTGATTATTTAGCGGTCGGAAAAGCGGACCCTAGGAAAATTGAGCAGATCGTGCAAGGGGTAGCGAACGGCTGTGTGCa                                                             >  2:2879144/1‑140 (MQ=255)
                                                                                cGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGt                                                           <  1:775319/140‑1 (MQ=255)
                                                                                cGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGt                                                           <  1:4560542/140‑1 (MQ=255)
                                                                                    gTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAACCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCAGTGAGCAGTCAgg                                                       >  2:4956386/1‑140 (MQ=255)
                                                                                      ttcGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCAGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGtt                                                     <  1:5459939/138‑1 (MQ=255)
                                                                                      tGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGtt                                                     <  1:1083010/140‑1 (MQ=255)
                                                                                      tGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGtt                                                     <  1:1027291/140‑1 (MQ=255)
                                                                                      tGCGATGTGTGTCAATGATGTGCGGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGtt                                                     <  2:1352843/140‑1 (MQ=255)
                                                                                         gATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAg                                                  <  2:368051/140‑1 (MQ=255)
                                                                                               tgtCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTTTTGCTTTTTTATCGCTGGGGAAAACGGACCCTAGGAAAAATTATCACATAGTACAAGGTGTATCCAAAGGATGTGTTGATTGAGGTTCATCCCTAg                                            >  1:3718599/1‑140 (MQ=255)
                                                                                               tgtCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTTTTGATTATTTTTCGGTCGGTAAAGCGGGCCCGAGGTAAATTGATCATATAGTTAAATGTGTTTCGAAAGGGTGTTAGCAGTGAGGTTGAGCCtttt                                            >  1:4468041/1‑138 (MQ=255)
                                                                                               tgtCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACCGCGGTGAGCAGTCAGGTTCAGCCTTAg                                            >  2:3499200/1‑140 (MQ=255)
                                                                                                     tgatgTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTATCGGGCGGAAAAGCGGACCCTAAGAAAAATTAGCACAACGTAAAACGTGTAGCGAAAGGCAGTGAGCAGTAAGGTTCAGCCTTATTcggcg                                      >  2:56696/1‑140 (MQ=255)
                                                                                                     tgatgTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTATTGATTATTTATCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTATCGAAAGGCTGTGAGCCGTCCGGTTAAGCCTTAGTcggcg                                      >  2:4880798/1‑140 (MQ=255)
                                                                                                     tgatgTGCTGGCACAAGGCGCAGAGCCGTGGTTTTTTATTGATTATTTAGCGGTCGGAAAAGCGGACCCAATGAAAAATGAGCACATCGTACAAGCTGTATCGAACGGCTGTGAGCAGTCAGGGTAAGCCTTAGTcggcg                                      >  2:2232707/1‑140 (MQ=255)
                                                                                                                    aGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTATCGGTCGGAAAAGCGGACCCTATGAAAATTGAACAGAACGTACAAAGTGTAGCGAAAGGCGGTGAGCAGTAAGGTTAAGCCATAGTCGGCGGCGAAAAGGCTgaga                       >  2:3607542/1‑140 (MQ=255)
                                                                                                                    aGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTgaga                       >  2:4294425/1‑140 (MQ=255)
                                                                                                                      gcgcAGAGCCGTTGTTTTTTGTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGGGTAGCGAAAGGCTGTGAGCAGTCAGGTTCAGCCTTAGGAGGCGGCGAAACGGCTGAGATg                     >  2:5536356/1‑140 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTTTTTTGCGTTAGGAAAAGAGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:3413180/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTCGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:2586922/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTCGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:3563775/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:2498723/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  2:520034/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:2287229/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:216063/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:2970070/140‑1 (MQ=255)
                                                                                                                       cgcAGAGCCGTTGTTTTTTCTTGATTATGTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGc                    <  1:4741999/140‑1 (MQ=255)
                                                                                                                         cAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCg                  <  1:1340381/140‑1 (MQ=255)
                                                                                                                            atccGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  2:935843/138‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  1:3768655/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  1:3161445/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  1:3903875/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  2:4148034/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  2:3781239/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  1:4810693/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  1:807038/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  2:117281/140‑1 (MQ=255)
                                                                                                                            agCCGTTGTTTTTTCTTGATGATTGAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGa               <  2:1562107/140‑1 (MQ=255)
                                                                                                                              ccGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGAtt             <  1:4651603/140‑1 (MQ=255)
                                                                                                                                 tttttttttttTGATTATTTAACGGTCGGAAAAGCGGCCCCTAATAAAATTTATCAGATAGTACAAGGGGTAGCGAACGGCTGTGAGCAGTCACGTTAAGCCTTAGTCGGCCGCGAAACCGCGGAGATGCCGGGATtata          >  2:903962/4‑140 (MQ=255)
                                                                                                                                 ttgttTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGATtata          <  1:287371/140‑1 (MQ=255)
                                                                                                                                 ttgttTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGATtata          <  1:4956386/140‑1 (MQ=255)
                                                                                                                                   gttTTTTGTTGATTATTTATCGGTCGGAAAAGCGGACCCGATGAAAATTGAGCAGATCGTACAAGGTGTATCGAACGGCGGTGAGCAGTCAGGTTCACCCTTATTCGGCGGCGAAAAGGCTGAGATGCCGGGATtatata        >  2:3218194/1‑140 (MQ=255)
                                                                                                                                     tttttCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGGCGGCGGCGAAACGGCTGAGATGCCGGGATTATATAca      <  2:1283406/140‑1 (MQ=255)
                                                                                                                                      ttttCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGATTATATAcag     <  2:4078070/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TTAATTTCGGGAACGGACGGTGTCGGCACCAAATTAAAGCTCGCTTTTTCCATGGATAAGCATGACACGATTGGCGTTGACGCTGTTGCGATGTGTGTCAATGATGTGCTGGCACAAGGCGCAGAGCCGTTGTTTTTTCTTGATTATTTAGCGGTCGGAAAAGCGGACCCTATGAAAATTGAGCAGATCGTACAAGGTGTAGCGAACGGCTGTGAGCAGTCAGGTTCAGCCTTAGTCGGCGGCGAAACGGCTGAGATGCCGGGATTATATACAGCAG  >  NZ_CP034943/686500‑686776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: