Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,530 A→C 6.2% Q22P (CAA→CCA)  EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5300AC 6.2% 789.8 / 5.2 305Q22P (CAA→CCA) EO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (208/60);  new base C (2/33);  total (210/95)
Fisher's exact test for biased strand distribution p-value = 4.02e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

TCACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAT  >  NZ_CP034943/755391‑755669
                                                                                                                                           |                                                                                                                                           
  aCCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAg                                                                                                                                           >  2:3492216/1‑140 (MQ=255)
               aGGGATGAGCTTTCCATGATTGATGCAATATGAAACGAAAAAAACGAGTGGGAATGAGAAATGGCAAATCAAATTTATCTTGCTGGCTATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGGGGGGGCa                                                                                                                              <  1:4877904/140‑1 (MQ=255)
                  gAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGtt                                                                                                                           >  1:3630922/1‑140 (MQ=255)
                  gAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGtt                                                                                                                           >  1:609164/1‑140 (MQ=255)
                     gagCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTtct                                                                                                                        >  2:192742/1‑140 (MQ=255)
                     gagCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCGTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTtct                                                                                                                        >  2:2988764/1‑140 (MQ=255)
                          gTCCATGCTTGATGCCATAGGAAACGAAAACAACGAGGGGCAAGGAGAAATCGCAAATCACAGTTATCTGGCGGGCGAGTCAACGGTTCACACGTATGCAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCt                                                                                                                   <  1:3490800/140‑1 (MQ=255)
                          gTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCt                                                                                                                   >  1:4475786/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGTTTCAACTGTTCAGACGTATGGAGACAGCAGAATTCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:2301996/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:4258529/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:4981057/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  2:4014145/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  2:5016732/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  2:5346236/1‑140 (MQ=255)
                           tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATGTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  2:2529716/1‑140 (MQ=255)
                                gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                             >  1:1883755/1‑140 (MQ=255)
                                gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                             >  1:5056908/1‑140 (MQ=255)
                                gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                             >  1:2559348/1‑140 (MQ=255)
                                gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATc                                                                                                             >  1:4506184/1‑140 (MQ=255)
                                gattgatGCCATATGAAACGAAAAAAACGAAGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                             >  1:3987747/1‑140 (MQ=255)
                                  ttgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTg                                                                                                           >  2:2302745/1‑140 (MQ=255)
                                   tgatGCCATAGGAACCGAAAAAAACGAGGGGGAATGAGAACTGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTACACACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                          <  1:1672178/140‑1 (MQ=255)
                                   tgatGCCATAAGAAACGAAAAAAACGACGGGGAATGAGAAAGTGCAAATCACATTTATCTTGCTGGCGCTTCAACTGTTCAAACGTATGGAGACAGCACACCTCCCCGGGGGCGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                          <  1:1772029/140‑1 (MQ=255)
                                    gttGCCATCTGAAACGAAAAAAACGAGGGTTAATGAGAAATGGCAAATCACTTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATCGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                         <  2:65923/138‑1 (MQ=255)
                                    gatGCCATACGACACGAAAAAAACGAGGGGGAATGATACATGGCACATCACATTTATCTGGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAACTCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                         <  1:1470250/140‑1 (MQ=255)
                                     atGCCATATGAAACTAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCTCATCTGCCg                                                                                                        >  2:3231055/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCACCTGTTCAAACGTATGGAGACAGCACCACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:4939812/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:582838/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:5169756/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:1082615/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:2081692/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:3766075/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:2647391/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:1344690/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:2003827/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:489665/1‑140 (MQ=255)
                                     atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:2807369/1‑140 (MQ=255)
                                     atGCCAGATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCAGATTTATCTTGCTCGCTATTCAACTGTTCAAACGTATGGATACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:2563778/1‑140 (MQ=255)
                                           tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCTGAGCat                                                                                                  >  2:5571996/1‑140 (MQ=255)
                                            atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                                 <  1:4717642/140‑1 (MQ=255)
                                            atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                                 >  1:356609/1‑140 (MQ=255)
                                               caaCGAAATCAACGTGGGGGTCTGATAATTTGCAATTCTCATTTTTCTTGTTGGCGTTTCAACTGTTCATACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:4219050/139‑1 (MQ=255)
                                               aaaCTAAAAAAACGAGTGTTAATGAGAAATGGAAAATCAGATTTATCTTGCTGTCTATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:4924118/140‑1 (MQ=255)
                                               aaaCTAAAAAAACGAGGGGGAATGAGAAATCGCAACTCACTTTTATCTTGCTGGCGATTCACCTGTTCAAACGTATGGAGACAGCACACCTCACGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:319364/140‑1 (MQ=255)
                                               aaaCGAAACAAACGAGGGCGAATGATAAATGGCAAATCACATTTATCTTGCTGGCTATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:4475786/140‑1 (MQ=255)
                                               aaaCGAAAAAAACGAGGGGTCATGAGAAATGTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:1560177/140‑1 (MQ=255)
                                               aaaCGAAAAAAACGAGGGGGAATGAGAAATGGAAAATCACATTTATCTTGCTGGCGATTCAACTGTTCCAACGTATGGAGACAGCACAAATCCAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:179704/140‑1 (MQ=255)
                                                aaCGTAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTTCTGGCGATGCAACTGGTCCAACGTATGGAGACAGCACAAATGAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCAGCTGGCGGAGCATCTTCa                                                                                             >  1:5371378/1‑140 (MQ=255)
                                                 aCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            >  2:4102970/1‑140 (MQ=255)
                                                  cGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATGTTCAAg                                                                                           >  2:5517803/1‑140 (MQ=255)
                                                    aaacaaacGAGGGGTCATGAGAAATTGAAAATCACATTTATCTTTCTGGCGATTCAACTGTTCAAACTTATGGAGACATCAAAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTg                                                                                         <  2:1824618/140‑1 (MQ=255)
                                                     aaaaaaCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGa                                                                                        >  2:4959990/1‑140 (MQ=255)
                                                         aaCGCGGGGGAATGCGAACTGGCAACTCACATTTATCGTGCTGGCGATTCAACCGTACAACCGTATGGAGACAGCACACCGCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                                    <  1:2471541/140‑1 (MQ=255)
                                                         aaCCAGGGCGTATGAGAAACGGCAAAACACATATATCTTGCGTGCGATTCAACTCTACACACGTATGGACACACCACCACTCCCCGGGGGGGCGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                                    <  1:4140045/140‑1 (MQ=255)
                                                                 ttaaTTAGATATGGCTTATCACATTTATCTTGGTGTCGTTTCTACTTTTCAAACGTTTGGAGACAGCAGAATTCCTGGGGGGGGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCaa                                                                            <  2:2451003/138‑1 (MQ=255)
                                                                  gAATGATAAATTGCTATTGACATTTATCTTGATTGCGATTAAACTGTTCAAACTTATGGAGACAGTACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCTCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAt                                                                           <  2:864452/140‑1 (MQ=255)
                                                                   taTGAGAATTGGCATCTCCCTTTTTTGTTGCTTGCTATTAACCTTTTCACGCGGATGCACTCACCCCATATCACGGGGCGAGGGGCCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATc                                                                          <  2:1298050/139‑1 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCTAGTGATTAACAGAGCAATCGGGGgacgaa                                                                >  1:1100287/1‑140 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagac                                                                >  1:1570531/1‑139 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                                >  1:892446/1‑140 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                                >  2:5143120/1‑140 (MQ=255)
                                                                                 aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTc                                                            >  2:5457929/1‑140 (MQ=255)
                                                                                 aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTc                                                            >  2:611029/1‑140 (MQ=255)
                                                                                 aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTc                                                            >  2:1243277/1‑140 (MQ=255)
                                                                                 aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGCGATTAACAGAGCAATCGGGGGAAGAAGCTc                                                            >  2:1724723/1‑140 (MQ=255)
                                                                                         ttATCTTGCTGTCGTTTCACTTGTTATACCGTCTGGAGAGATCACCCCTCACGGGGGGTGGGGGGAGTTTCTCGGCTCGAATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAt                                                    <  2:5645132/140‑1 (MQ=255)
                                                                                                 ctttcGATTAAACTTTTCAAACGTATTGAGACAGATCAAATCATGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            <  2:3914826/136‑1 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAACACATTTGTGGac                                            >  1:5709542/1‑139 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGTaa                                            >  2:2395562/1‑140 (MQ=255)
                                                                                                 cTGGCGATACAACCGTTCAAACGCAGGGAGACAGCACCACTCCAGGGGGCTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            <  1:4993506/140‑1 (MQ=255)
                                                                                                 cTGCCGATTCACCTGGACCACAGTCTGTAGACAGCCCACCTCAGGGGGGGTGGGGGCAGTGTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            <  2:2000581/140‑1 (MQ=255)
                                                                                                      taTTCAAGTGTTCAAACCTCTGGCGACAGCACAAATCATGGGGGGTGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAggg                                       <  2:5204662/139‑1 (MQ=255)
                                                                                                      gATTCAACTGTACAAACGTATGTAGACAGCACAAATCCCGGGGGGTGGGGGGAGTTTCTCGGCTCGCATCTGCCTGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAggg                                       <  2:3321581/140‑1 (MQ=255)
                                                                                                       tttCATCTGTTCATACTTATGGAGACAGCACATACCCTGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGc                                      <  2:5122728/139‑1 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGATCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:5562666/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCCGGCTTCAg                             >  1:2123145/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCCGGCTTCAg                             >  2:4804234/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:1211358/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  2:4827963/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCAGCCGGAGCATCTTCAAGTGATTAACAGAGCACTCGGGGGAAGAAGCTCGAAAACATTGGTGGAAGAGGGCAGTCTTCAg                             >  2:288834/1‑140 (MQ=255)
                                                                                                                  ccaccGTATGGAGACAGCCCAACTCCCGGGGGGTGGGGGCAGTGTCTCGCCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           <  1:2950397/136‑1 (MQ=255)
                                                                                                                  cAAACGTATGGAGACATCACAAATCATGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATCAACAGAGCGATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           <  2:5404382/140‑1 (MQ=255)
                                                                                                                       gTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTc                      >  2:2035709/1‑140 (MQ=255)
                                                                                                                          tGGAGATAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  2:768242/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  1:269036/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  2:4672932/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                          >  1:1711304/1‑46 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGACGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:1459754/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:3303139/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:3260999/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:3386558/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:3672919/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:1387681/1‑140 (MQ=255)
                                                                                                                                  gCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtt           >  1:5408117/1‑140 (MQ=255)
                                                                                                                                   cacaAATCCAGGTGTGTGGGTGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          <  2:3614330/140‑1 (MQ=255)
                                                                                                                                   cacaAATCATGGCGGGTGGGTGCAGTTTCTCGGCTAGCATCTGACGGAGGATATTCAAGTGATTAACAGAGAAATAGGTGCAAGAAGATACAAAAAATATGTGGAAGAGAGAAGGATTCAGTAGATTCTTGATGTGATTg          >  2:798586/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  1:1023314/58‑1 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            >  2:1023314/1‑58 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  1:213136/1‑140 (MQ=255)
                                                                                                                                           aaGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAt  >  1:280970/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAT  >  NZ_CP034943/755391‑755669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: