BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	767,723	A→C	6.5%	I81S (ATT→AGT)	EO946_RS03930 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	767,723	0	A	C	6.5%	883.5 / 5.8	356	I81S (ATT→AGT)	EO946_RS03930	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (162/152); new base C (6/34); total (170/186)
Fisher's exact test for biased strand distribution p-value = 7.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

CTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAA  >  NZ_CP034943/767587‑767861

cTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCCGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCACGCACAGAGCCGCCTCCAATGc                                                                                                                                         >  2:2815341/1‑140 (MQ=17)

 tGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCTGCCAATACCAGGGCCGCCGCCGCCGCCCACCCCAGCCACACACCCGCCGCCATATGCAGGGCCTGAGACGCCTCCGACGCCAGGCACAGGGCCGATAGCAATggg                                                                                                                                        >  2:404574/1‑138 (MQ=2)

 tGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCGGATGCCCGGCACCGCGCCGCCGCCCATACCAGCGCCGGAGCCGCGGCCGACGCCCGGCACCGCGCCGCGAGCaagccc                                                                                                                                        >  2:3270667/1‑136 (MQ=17)

 tGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGcc                                                                                                                                        >  2:3833488/1‑140 (MQ=255)

      cgcACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTTCCAGCGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCAATGCCCGGgc                                                                                                                                   >  1:4430216/1‑140 (MQ=14)

        cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCACCCAATGCCAGGgccg                                                                                                                                 >  2:4681351/1‑140 (MQ=255)

        cACCGACGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccg                                                                                                                                 <  1:5485245/140‑1 (MQ=255)

              cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  1:969271/140‑1 (MQ=255)

              cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  1:5277417/140‑1 (MQ=255)

              cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGCCCGGAGCCGCCGCCGAAGCCAGGCTCAGCGCCGCCGCCAATACGAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGGGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  1:4893791/140‑1 (MQ=255)

              cgAAGCCAGGCACAGCGCCGCCGCCAAGCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:5637243/140‑1 (MQ=255)

                   ccAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGcc                                                                                                                      <  1:4865808/140‑1 (MQ=255)

                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTCCCAGGGCCCGCGCCGCCGCCGACGCCACGCACAGCGCCGCAACCAATCCCAGGGCCGCCCCCCACGCCAgcc                                                                                                                  >  2:3518756/1‑138 (MQ=17)

                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCCGGGCCGCCGCCGAAGCAAGGc                                                                                                                  >  1:2646229/1‑140 (MQ=255)

                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCCATGCCAGGGCCGCCGCCGAAGCCAGGc                                                                                                                  >  2:1612031/1‑140 (MQ=255)

                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCCCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGc                                                                                                                  >  2:5279827/1‑140 (MQ=255)

                        cacaGCGCCGCCGCCAATACCACGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGca                                                                                                                 <  2:295079/140‑1 (MQ=255)

                           aGCGCCGCCGCCAAGACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAg                                                                                                              <  1:3390672/140‑1 (MQ=255)

                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAgcg                                                                                                            >  1:2114919/1‑140 (MQ=255)

                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCCGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAgcg                                                                                                            >  1:1685406/1‑140 (MQ=255)

                               ccgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgcc                                                                                                          <  1:1397142/140‑1 (MQ=255)

                                cgccgccAAGTCCAGGGCGGCTGCCGCCGACGAAGCCAGGGACCGCGCCGCCGCCAATACCCGGGCCGGAGCCGCCGCCGAAGCGAGGCACAGCGCGGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                         <  2:4076472/140‑1 (MQ=18)

                                 gccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgcccc                                                                                                        >  2:3458670/1‑138 (MQ=255)

                                  ccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                         <  1:4125869/138‑1 (MQ=255)

                                     ccAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAAGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  2:2172251/140‑1 (MQ=255)

                                     ccAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  2:4636309/140‑1 (MQ=255)

                                     ccAATACCAGGGCGGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  1:3814235/140‑1 (MQ=255)

                                     ccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  1:3533367/140‑1 (MQ=255)

                                     ccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  1:4820597/140‑1 (MQ=255)

                                           cgaGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCCGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGcc                                                                                              <  2:4416628/138‑1 (MQ=255)

                                              gggCCGGAGCCGCCGCCGTAGCCAGGCACCGCGCCGGCGCCAATACCAGGGCCGGAGGCGCCGCCGAAGCCGGGCACCGCGCCGCGCCCACTGCCAGGGGCGCGGCCGCCGCCCTGCCCGGCGCCGGGCCCGATGCCAgg                                                                                           >  2:2259411/1‑140 (MQ=9)

                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGg                                                                                          <  1:778733‑M2/140‑2 (MQ=255)

                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGg                                                                                          <  1:5313297‑M2/140‑2 (MQ=255)

                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGg                                                                                          <  1:2172503‑M2/140‑2 (MQ=255)

                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGCCACAGCGCCGCAACCAAAGCCAGGg                                                                                          <  2:1798453‑M2/140‑2 (MQ=255)

                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCACCCAATGCCCGGGCAGGAGACGCAGGCGGGCCCAGGGCCGCGACCCCTGCCAggg                                                                                          >  2:751002/1‑140 (MQ=14)

                                                   ggAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGACGCCCGGCACAGCGCCGCTCCCACTGCCAGGgccgc                                                                                      <  2:4135940‑M2/140‑6 (MQ=255)

                                                   ggAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCCCAGCCCCGCTACCAATGCCAGGgcccc                                                                                      <  2:4511684‑M2/140‑6 (MQ=255)

                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                         >  2:3376567/1‑120 (MQ=255)

                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGCCCCGGAGCCGCCGCCGAAGCCAGGCACAGCGACGCTACCAATGCCAGACACGCCGCCCCACCCAGGCACAGCGACGCTACAAATGCCAGGaccgac                                                                                     <  1:4290838‑M2/140‑7 (MQ=255)

                                                         gccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCACGCACAGCGCCGCTACCAATCCCAGGgccgccgccgc                                                                                <  1:3758847‑M2/140‑12 (MQ=255)

                                                            gccgACGCCCGGCACAGCGCCGCCGCCAATACCAGTGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccac                                                                             >  2:2196664‑M2/1‑126 (MQ=255)

                                                            gccgAAGCCAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgc                                                                             <  1:1163893/140‑1 (MQ=255)

                                                            gccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGACCCCAGGCACAGCGCCGCTACCAAGGCCAGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgc                                                                             <  2:1884411/140‑1 (MQ=255)

                                                            gccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGgccgccgccgccgc                                                                             <  1:2267656/140‑1 (MQ=255)

                                                              cgactccATGCCCAGCGCCGCCGCCACTCCCAGGGCGCGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccgccgccg                                                                           <  2:1188232/135‑1 (MQ=17)

                                                              cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccgccgccg                                                                           <  2:5770787/140‑1 (MQ=255)

                                                              cgAAGCCAGGCACAGCCCCGCCGCCACTCCCCGGGCCGGAGCCGCCGCCGAAGCGAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccgccgccg                                                                           <  2:1355869/140‑1 (MQ=18)

                                                                   ccAGGCACAGCGCCGCCGCCAAGACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGcc                                                                      <  1:690120/140‑1 (MQ=255)

                                                                     aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAg                                                                    >  2:322370/1‑140 (MQ=255)

                                                                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCCGGc                                                                  >  1:5217325/1‑140 (MQ=255)

                                                                        ccccgccccgccgccAAACCCAGGGCCGGCGCCCCCGCCGAAGCCAGGCACAGCGCCGCCACCACTCCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGca                                                                 <  2:1667715/133‑1 (MQ=18)

                                                                           aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCCGGCACAg                                                              >  1:5174741/1‑140 (MQ=255)

                                                                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcc                                                            >  2:2428115/1‑139 (MQ=255)

                                                                             cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCAAGGCCAGGGCCGCCGCCGCCCCCGAACCCAGCCACAgcg                                                            >  2:247884/1‑140 (MQ=18)

                                                                               ccgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTCCCAAGGCCAGTGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgcc                                                          >  2:4927477/1‑140 (MQ=255)

                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         <  2:2584291/140‑1 (MQ=255)

                                                                                cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         <  2:2657834/140‑1 (MQ=255)

                                                                                       aaTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCCGCCACAGCGCCGCCGCCaa                                                  >  2:795739/1‑140 (MQ=255)

                                                                                         tACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATg                                                >  1:5653582/1‑140 (MQ=255)

                                                                                         tACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCACTGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATg                                                >  1:2573019/1‑140 (MQ=255)

                                                                                           ccAGGGGGGGAGGCGCCGCCGACCCCATGCACAGCGCCTCTACGACTGCGAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              <  2:2968079/140‑1 (MQ=18)

                                                                                           ccAGGGCCGGAGCCGCCGCCGTCGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              <  2:4786134/140‑1 (MQ=255)

                                                                                            cAGGGCCGGAGGAGCCGCCGCACCCAGCCACACCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCTCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCa                                             <  1:2892404/140‑1 (MQ=18)

                                                                                             aGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAgcgc                                                                                                           >  1:4946678/1‑77 (MQ=255)

                                                                                             aGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAgcgc                                                                                                           <  2:4946678/77‑1 (MQ=255)

                                                                                             aGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTGCCAg                                            >  1:335589/1‑140 (MQ=255)

                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACCGCGCCGCCGCCAAGGCCCggg                                          >  2:5097770/1‑140 (MQ=255)

                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  2:5347732/1‑140 (MQ=255)

                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  2:4261998/1‑140 (MQ=255)

                                                                                               ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  1:861369/1‑140 (MQ=255)

                                                                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGCCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCggc                                     >  2:1099640/1‑139 (MQ=255)

                                                                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCCCCGCCGCCAATGCCAGGGCCGGa                                     >  2:1047135/1‑140 (MQ=255)

                                                                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCCCTACCAAAGCCAGGGCCCCCGCCGCCGCCGAAGCCAGGCACACAGCCGCCGCCAAAGCCAgcccccga                                     >  2:855057/1‑133 (MQ=18)

                                                                                                    gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGCCACAGCGCCGCCGCCAATGCCAGCGCCGGa                                     >  2:3948257/1‑140 (MQ=17)

                                                                                                    gAGCCGCCGCCGAAGCCAGGCACAGCCCCGCAACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGGCCCCCGCGCCCGCCCAAGCCACGCACACCGCCCCCCCCAATGCCAGGGACGGa                                     >  1:5723906/1‑140 (MQ=18)

                                                                                                     aGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCCGGGCCGCCGCCGCCGCCGCAGCCGGGCCCAGAGCCGCCGCCAGTGCCAGGGCCgggg                                    >  2:624145/1‑138 (MQ=18)

                                                                                                          ccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgcc                               >  2:5156933/1‑140 (MQ=255)

                                                                                                            gccgTAGACACGCACCGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCTCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccgc                             <  1:1376227/140‑1 (MQ=21)

                                                                                                            gccgAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccgc                             <  1:4495150/140‑1 (MQ=255)

                                                                                                            gccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccgc                             <  1:2971126/140‑1 (MQ=255)

                                                                                                              cgACGCCAGGCACAGCCCCGCGACCACGGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCACGGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:3141117/140‑1 (MQ=18)

                                                                                                              cgAAGCCAGGCACAGCGCCGCTACCCATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgaagccaggcacagcgccgccgccaatgccagggccggagccgccgccgaag                           >  1:1898738‑M2/1‑76 (MQ=255)

                                                                                                              cgAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:1825454/140‑1 (MQ=255)

                                                                                                              cgAAGCCAGGCACAGCGCCGCTACCAATGCGCGGGCCGCCGCCGAAGCGAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:3068617/140‑1 (MQ=255)

                                                                                                                 cgCCATGCACAGCCCCGCGTCCACGCCCCGGGGCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAg                        <  2:1990553/139‑1 (MQ=14)

                                                                                                                      ggCACAGCGCCGCGTCCAATGCCAGGGCCGCAGCCGAAGCCAGGCACAGCCACTGGCCCAATCCCACGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCATCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAgg                   <  2:162881/140‑1 (MQ=17)

                                                                                                                       gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGc                  >  2:4904160/1‑140 (MQ=255)

                                                                                                                        cacaGCGCCGCTAACCCGGCGAGGGCCGCCGCCGAAGCCAGCCACACCCCCGCCACCACCCCCAGGGCCGGCGCCGCCGCCGAAGCCAGGCCCAGCGCCTCCGGCACGCCGAGGGCCGGCGCCGCCGCCGAAGCCAGGca                 <  1:1013645/140‑1 (MQ=9)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:4063597/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            <  2:317464/140‑1 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  2:3773357/1‑140 (MQ=255)

                                                                                                                               ccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGACGCCGCCGAACCCAGGCACAGcgcc          >  2:1764895/1‑140 (MQ=255)

                                                                                                                                 gcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgc        >  2:3692153/1‑140 (MQ=255)

                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGcaca                                                                                                               >  1:4681182/1‑36 (MQ=255)

                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACTGCGCCGCCGCCAATGCCCGTGCCCGATCCGCGGACCAAGCCAGGCCCAGcgcagcc       >  1:253482/1‑140 (MQ=18)

                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           >  1:4713075/1‑120 (MQ=255)

                                                                                                                                  cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgcc       >  1:5010883/1‑140 (MQ=255)

                                                                                                                                     ccAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgaagccaggcacagcgccgccgccaatgccagggccggagccgccgccgaagccaggcacagcgccgccgccaat    <  2:2024787‑M2/140‑88 (MQ=255)

                                                                                                                                       aaTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCTCCGCCAATGCCAGTGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCCGCCaa  >  2:3031458/1‑140 (MQ=255)

CTGCGGCGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAA  >  NZ_CP034943/767587‑767861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`