BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	767,765	A→C	6.1%	I67S (ATT→AGT)	EO946_RS03930 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	767,765	0	A	C	6.1%	960.3 / 11.0	363	I67S (ATT→AGT)	EO946_RS03930	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (182/142); new base C (4/32); total (187/176)
Fisher's exact test for biased strand distribution p-value = 1.91e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

ACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGATATTCGTATTC  >  NZ_CP034943/767629‑767904

 ccAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACGAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGcc                                                                                                                                         <  2:1627313/140‑1 (MQ=255)

     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGg                                                                                                                                     <  1:5313297‑M2/140‑2 (MQ=255)

     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGg                                                                                                                                     <  1:2172503‑M2/140‑2 (MQ=255)

          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGCCCCGGAGCCGCCGCCGAAGCCAGGCACAGCGACGCTACCAATGCCAGACACGCCGCCCCACCCAGGCACAGCGACGCTACAAATGCCAGGaccgac                                                                                                                                <  1:4290838‑M2/140‑7 (MQ=255)

            gccgccgccGAAGCGAGGCACAGCGCCGCCGCCAATCAGAGGGCCGGAGGCGCCGCCGAAGCCAGGCACAGCGCCGCTAGCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgc                                                                                                                              >  1:5697173‑M2/1‑132 (MQ=255)

               gccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgc                                                                                                                           <  1:1230851‑M2/140‑12 (MQ=255)

               gccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGgccgccgccgc                                                                                                                           <  2:5764484‑M2/140‑12 (MQ=255)

                  gccgACGCCCGGCACAGCGCCGCCGCCAATACCAGTGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccac                                                                                                                        >  2:2196664‑M2/1‑126 (MQ=255)

                  gccgAAGCCAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgc                                                                                                                        <  1:1163893/140‑1 (MQ=255)

                  gccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGgccgccgccgccgc                                                                                                                        <  1:2267656/140‑1 (MQ=255)

                  gccgAAGCCACGCACCGCGCCCCCGCCAAGCCCAGGGCCGGAGGCGCCGCCGACGCCAGGCACAGCGCCGCTCCCACGGCCAGGGCCGCCGCCGAACCCAGGCACAGCGCCGCTACCAATGCCCGGgccgccgccgccgc                                                                                                                        <  2:4668122/140‑1 (MQ=17)

                    cgtacacAGCCAAAGCTCCGCCGCCATGCCCAGGCGCCGTGCCGGCGCCCCCGCCAGGCACAGCGCCGCTACCAACGCCAGGGCCCCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccgccgccg                                                                                                                      <  2:5486292/134‑1 (MQ=16)

                    cgAAGCCAGGCACAGCGCCGCCGCCAAGACCAGCGCCGGAGCCGCCGCCGAAGCCAGCCACAGCGCCGCGACCAATGCCAGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccgccgccg                                                                                                                      <  2:1308552/140‑1 (MQ=17)

                         ccAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGcc                                                                                                                 <  1:740385/140‑1 (MQ=255)

                           aGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCCCAACCAATGCCAGCGCCGCCGCCGAAGCCAGGCACAGCGACGCGACCAATGCCACGGCCGCCGCCGCCGCCGAAGcccg                                                                                                               >  2:3267144/1‑138 (MQ=18)

                              ccccgccccgccgccAAACCCAGGGCCGGCGCCCCCGCCGAAGCCAGGCACAGCGCCGCCACCACTCCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGca                                                                                                            <  2:1667715/133‑1 (MQ=18)

                                 aGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAg                                                                                                         >  2:932527/1‑140 (MQ=255)

                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcg                                                                                                       >  2:5237893/1‑140 (MQ=255)

                                   cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcc                                                                                                       >  2:2428115/1‑139 (MQ=255)

                                   cgccgccgcCAATACCAGGGCCCGAGCCGCCCCCGAAGCCAGGCACATCGCCGCTACCAATGCCAGCGCCGCCGACGAAGCCAGGCACAGCGCCGCTACCAATCCCAGCGCCACAGCCGCCGCCAAACACAGCAACAgcg                                                                                                       >  1:816719/1‑140 (MQ=18)

                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                    <  2:2657834/140‑1 (MQ=255)

                                      cgccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                    <  1:4354648/140‑1 (MQ=255)

                                        ccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccgcc                                                                                                  >  2:5130023/1‑140 (MQ=255)

                                        ccgccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGCGCCTCCCCCGAAGCCAGGCACACCGCCGCTACCAAAGCCAGAGCCCCCGCCGCCGCCGAACCCAGGCACACcgccgcc                                                                                                  >  1:4707775/1‑140 (MQ=21)

                                               tACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCCGGCACAGCGCCGCCGCCAATg                                                                                           >  1:5260237/1‑140 (MQ=255)

                                               tACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGCCCACTGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATg                                                                                           >  1:2573019/1‑140 (MQ=255)

                                                 ccAGGGGGGGAGGCGCCGCCGACCCCATGCACAGCGCCTCTACGACTGCGAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                                                                         <  2:2968079/140‑1 (MQ=18)

                                                 ccAGGGCCGGAGCCGCCGCCGTCGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                                                                         <  2:4786134/140‑1 (MQ=255)

                                                 ccAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                                                                         <  1:1386788/140‑1 (MQ=255)

                                                   aGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTGCCAg                                                                                       >  1:335589/1‑140 (MQ=255)

                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                                                                     >  1:861651/1‑140 (MQ=255)

                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCCCCGCCGCCAAGGCCAggg                                                                                     >  2:695327/1‑140 (MQ=255)

                                                     ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACAAATGCCAGTGCCCCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                                                                     >  1:2876626/1‑140 (MQ=255)

                                                          gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGCCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCggc                                                                                >  2:1099640/1‑139 (MQ=255)

                                                           aGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCCAACCCAGGCACAGCGCCGCCCCCAATGCCAGGGCCGGac                                                                               >  2:3443433/1‑139 (MQ=255)

                                                               gccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTTCCAATGCCAGGGCCTCCGCCGCCGCCGAAGACAGGGACAGCGCCGCCGCCAATGCCAGGGCCGGagcagc                                                                           >  2:4204043/1‑140 (MQ=18)

                                                                  gccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccgc                                                                        <  1:1084435/140‑1 (MQ=255)

                                                                  gccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAgccgccgc                                                                        <  1:3796486/140‑1 (MQ=255)

                                                                    cgAAGCCATGGACAGCTCCTCTACCAAGGCGAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                                                                      <  1:980541/140‑1 (MQ=18)

                                                                    cgAAGCCAGGCACAGCGCCGCTACCCATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgaagccaggcacagcgccgccgccaatgccagggccggagccgccgccgaag                                                                      >  1:1898738‑M2/1‑76 (MQ=255)

                                                                    cgAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                                                                      <  1:1825454/140‑1 (MQ=255)

                                                                    cgAAGCCAGGCACAGCGCCGCTACCAATGCGCGGGCCGCCGCCGAAGCGAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                                                                      <  1:3068617/140‑1 (MQ=255)

                                                                    cgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                                                                      <  1:2498142/140‑1 (MQ=255)

                                                                    cgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                                                                      <  1:5130023/140‑1 (MQ=255)

                                                                       ccccAGGAACAGCCCCGCTACCACGGCCCGGCCCGCCGCCGAAGCCCGGCACAGCGCCGCTACCACTGCCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAg                                                                   <  2:2574802/138‑1 (MQ=17)

                                                                         ccAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGcc                                                                 <  1:5176705/140‑1 (MQ=255)

                                                                             gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGc                                                             >  2:4904160/1‑140 (MQ=255)

                                                                                 aGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCCCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGACGCCGCCAATGCCAGCGCCGGAGCCCCCGAAGAAGACAGGCACAg                                                         >  2:5274728/1‑140 (MQ=21)

                                                                                   cgccgcTACCAATGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg                                                       >  2:4317370/1‑140 (MQ=255)

                                                                                   cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg                                                       >  1:4452586/1‑140 (MQ=255)

                                                                                   cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg                                                       >  2:2766317/1‑140 (MQ=255)

                                                                                   cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg                                                       <  2:317464/140‑1 (MQ=255)

                                                                                   cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg                                                       >  1:4063597/1‑140 (MQ=255)

                                                                                      cgcgACCCATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  2:3813352/140‑1 (MQ=255)

                                                                                      cgcgACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  2:179878/140‑1 (MQ=255)

                                                                                      cgcTACCCATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCACGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  1:5342373/140‑1 (MQ=255)

                                                                                      cgcTACCACTGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGACGCTACCAAGGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGGACAGCGACGCCGCCACTGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    <  1:624145/140‑1 (MQ=18)

                                                                                      cgcTACCAATGCCAGGGCCGCCGCCGAAGCCCGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    >  2:1058518/1‑140 (MQ=255)

                                                                                       gcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCTCAGCGCCGCTACCAATGCCAGGgccgccgccgccgaagccaggcacagcgccgccgccaaagccagggccggagccgccgccgaagccaggcacagcgccgccgc                                                   <  2:2485705‑M2/140‑84 (MQ=255)

                                                                                       gcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgc                                                   >  2:3692153/1‑140 (MQ=255)

                                                                                          acccctGCCAGGGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCCATGCCACGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc                                                <  2:4430216/135‑1 (MQ=18)

                                                                                          accacggCCAGGGGCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc                                                <  2:794286/134‑1 (MQ=255)

                                                                                          aCCAATGCCCGGGCCGCCGCCGAAGCCACGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc                                                <  1:296749/140‑1 (MQ=255)

                                                                                           ccAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgccgaagccaggcacagcgccgccgccaatgccagggccggagccgccgccgaagccaggcacagcgccgccgccaat                                               <  2:2024787‑M2/140‑88 (MQ=255)

                                                                                            cactGCCATGGACGTCGCCGAAGCCAGGGACAGCGACGCTTCCTCTGCCAGGGAGGCCGGCGGCGGCGAAGCGAGGCACATCGCCTCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCa                                              <  2:3725242/137‑1 (MQ=17)

                                                                                            cAATGCCACGGCCGCCGCCGAAGCCAGGCACAGCGCCTCTACCACAGCCACGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCa                                              <  2:5065617/140‑1 (MQ=18)

                                                                                             aaTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCaa                                             >  2:844135/1‑140 (MQ=255)

                                                                                               tGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGcc                                                                                                                                         <  1:1491780/46‑1 (MQ=255)

                                                                                                gccctggCCGCCGCCGCAGCCAGGCACCGCGCCTGTACCAGTGCCTGGGCAGGCGCCGCCGCCGAAGACAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGc                                          <  1:3420240/135‑1 (MQ=17)

                                                                                                gCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGc                                          <  1:4860443/140‑1 (MQ=255)

                                                                                                   aGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                                                                    <  1:4614110/79‑1 (MQ=255)

                                                                                                    cggCCGCCGCCGAAGCCACGCACAGCGCCGCTACCCATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGCCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGa                                      <  1:4204043/139‑1 (MQ=255)

                                                                                                     ggCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACTGCGCCGCCGCAAATGCCAGGCCCGGAGACGCCGCCGAAGACAGCCACAGCCCCGCCTACAATCCCAGAc                                     >  1:1531908/1‑140 (MQ=17)

                                                                                                     ggCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGAc                                     >  1:3698769/1‑140 (MQ=255)

                                                                                                          ccgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATgg                                >  1:2117199/1‑140 (MQ=255)

                                                                                                           cgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATggg                               >  2:3582876/1‑140 (MQ=255)

                                                                                                           cgccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATggg                               >  2:1798120/1‑140 (MQ=255)

                                                                                                             ccgACGCCAGGCCCCGCGCCGCTTCCAAGGCCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAAGGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTa                             <  2:1562391/140‑1 (MQ=17)

                                                                                                             ccgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTa                             <  2:1493860/140‑1 (MQ=255)

                                                                                                                  gCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCAt                        <  1:3546794/140‑1 (MQ=255)

                                                                                                                  gCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCAt                        <  2:5799847/140‑1 (MQ=255)

                                                                                                                  gCCAGGCACAGCGCCGCCACCAATGCCAGGGCCGCCGCCGCCGCCGCAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCAt                        <  2:2876626/140‑1 (MQ=255)

                                                                                                                       gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCCCAGCGCCGCCGCCAGTGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGCGTAACCAGCTGGa                   >  2:3325673/1‑140 (MQ=255)

                                                                                                                       gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGa                   >  2:4582687/1‑140 (MQ=255)

                                                                                                                       gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGa                   >  1:1004468/1‑140 (MQ=14)

                                                                                                                        cacaGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGAt                  <  1:3157848/140‑1 (MQ=255)

                                                                                                                        cacaGCGCCGCGACCAATGCCAGCGCCGGCGCCGCCGCCGAAGCCAGGCACCGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGAt                  <  1:4949819/140‑1 (MQ=255)

                                                                                                                           aGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAACCCAGGCACAGCGCAGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGACGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATCGGGACCCAACTGGAGAAc               >  2:4026942/1‑140 (MQ=18)

                                                                                                                            gcgcCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACg              >  2:3480131/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccg                                                    >  2:1887398/1‑101 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGa             >  2:4501/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGa             >  2:4114995/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGa             >  2:391189/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGa             >  1:680532/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGAACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGa             >  2:1410464/1‑140 (MQ=255)

                                                                                                                             cgccgcTACCAATGCCAGGGCCGCCGCCGCCCCCGAAGCCCGCCACAGCGCCGCCGCCAATGCCAGGCCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCCCCAAGGCCAGACCCTGGGTAACCATCTGGATAACGa             >  1:5526909/1‑140 (MQ=21)

                                                                                                                                cgcTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGatat          >  2:2347409/1‑140 (MQ=255)

                                                                                                                                   tACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGATATTCg       <  1:1625163/140‑1 (MQ=255)

                                                                                                                                   tACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGATATTCg       <  1:5564395/140‑1 (MQ=255)

                                                                                                                                     ccAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGATATTCGTa     >  2:2079115/1‑140 (MQ=255)

ACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGACCATGGGTAACCATCTGGATAACGATATTCGTATTC  >  NZ_CP034943/767629‑767904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`