Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 803,278 G→T 6.3% Q29K (CAG→AAG)  yfmC ← Fe(3+)‑citrate ABC transporter substrate‑binding protein YfmC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943803,2780GT 6.3% 1117.5 / 13.1 421Q29K (CAG→AAG) yfmCFe(3+)‑citrate ABC transporter substrate‑binding protein YfmC
Reads supporting (aligned to +/- strand):  ref base G (177/203);  new base T (37/2);  total (216/205)
Fisher's exact test for biased strand distribution p-value = 7.99e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 451 total aligned reads displayed.

AGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAACAC  >  NZ_CP034943/803139‑803417
                                                                                                                                           |                                                                                                                                           
agaTTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTctg                                                                                                                                             >  2:4051130/1‑140 (MQ=255)
  aTTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCGTCGTGGTGGATCACACTATAATCCGTAGACTTGCGTTCGCTGCTTGCGCGGTTATTgtggg                                                                                                                                           >  2:1625536/1‑139 (MQ=255)
          cAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGaa                                                                                                                                   <  1:893360/140‑1 (MQ=255)
           aGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGGGCAGGATCACGCTAGAATCCATACACTAGCTTTCCCTCCATACGCTGTTATTGTGGCAGgaagaaa                                                                                                                                  >  2:5290680/1‑139 (MQ=255)
            gCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACa                                                                                                                                 >  2:258683/1‑140 (MQ=255)
            gCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACATCGTGGGGCTGGATGACGGGATAAACCTTATAAGTGCTTTCGCTTCGTGCGCTGTTATTCTGGCTGGATGAACa                                                                                                                                 >  1:1271119/1‑140 (MQ=255)
            gCATCCAAGAATGAAAGCTCAAGGACAACCACTCGGTTGGGTGTGCCGCTTACTGTCGTGTTTGGTGCGTGGTGGAGGGTCACGGTAGAATCCATAGACTTGCTTTCGCGGCTTGCGCGGGTCTTCTGGCGGGATGaaga                                                                                                                                 >  1:2478416/1‑138 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTATAATACTTAGACTTGCTTTAGCTGCTTACGCTGTTATTCTGGCTGGATGAACAg                                                                                                                                >  2:1848719/1‑140 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTAGCTGCTTCCGCTGTTATTGTGGGTGGATGAACAg                                                                                                                                >  2:4356301/1‑140 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCGTCTTCGTGCTGGATCACTCTAGAATCCGTAGACTTGCTTTAGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAg                                                                                                                                >  2:3107736/1‑140 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCATATTCGGGCTGGGTCACTCTAGAATCCTTAGACGTTCTTTCGCTGCTTCCGCTGTTATTCTGGGTTGATGAACAg                                                                                                                                >  2:1764417/1‑140 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACATATTCGTGCTGGTTCACTCTAGAATCCTTAGACTTTCTTTCGCTGCTTCCGCGGTTATTATTGGTGGATGAACAg                                                                                                                                >  2:2310040/1‑140 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCTTCTTCGTGCTGGGTGACGCTACAATCCGTAGACTTTGGTTGGCTGGTTGCGGTTTTATTGTGggtgggtgtaaac                                                                                                                                >  1:4872851/1‑135 (MQ=255)
             cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCATATTCGTGCGGGGACACGCTAGCATGCATAGACGTGCTGTCGCTGCTTCCGCGGGTATTTTGGCTGGATGAACAg                                                                                                                                >  2:3701263/1‑140 (MQ=255)
              aTCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGc                                                                                                                               <  1:5583522/140‑1 (MQ=255)
                ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTATGGCTGGATGAACAGCCg                                                                                                                             >  2:5011820/1‑140 (MQ=255)
                ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCGTCTTAGTGCTGGGTCACTCTAGAATCCGTAGACGTGCTTTCGCTGCTTCCGCGGTTATTCTGGCTGGATGAACAGCCg                                                                                                                             >  2:1943685/1‑140 (MQ=255)
                ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCGTGTTGGGGCGGGCTGACTCTAGAATCCGTGGTCGTGGTTGATCTGCGTGCGCTGTTATTATTGGTGGGTTAAAAGCCg                                                                                                                             >  1:5305399/1‑140 (MQ=255)
                  aaGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGa                                                                                                                           <  2:4849210/140‑1 (MQ=255)
                       tGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTGCTTCTTCGTGCTGGGTGACTCGAGAATCCTTAGACTTGCGTTAGCTGCTTCCGCGGTTATTATGGGGGGGTGAACAGCCGGATACaa                                                                                                                      >  1:3819719/1‑140 (MQ=255)
                       tGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCCCTCTAGAATCCTTTGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACaa                                                                                                                      >  2:5673992/1‑140 (MQ=255)
                       tGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCTTGGTTGTGGTGGAGCACTCGATAAAACTTAGACTTTGATTCGCTGCGTCCGCGGGTATTGTGGCTGGATGAACAGCCGGATACaa                                                                                                                      >  1:5786182/1‑140 (MQ=255)
                       tGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCGTCTTCGTGGTGGGTCCCTCGAGTAAACTTAGAATTGCTTTGGCTGCTTGCCCAGTTATTCTGGCTGGATGTACAGCCGGATACaa                                                                                                                      >  1:5480519/1‑140 (MQ=255)
                         aaaGCTCAAGGACAACCACGCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCTTCTTCGTGCTGGATCCCTCTAGCATCCTTAGGCGTGGTTTAGCTGCGTCCGCTGGTATTGTGGCTGGATGCACCGGCGGATACaaat                                                                                                                    >  2:5195231/1‑138 (MQ=255)
                                    gagacagcTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGAt                                                                                                         <  1:2962990/133‑1 (MQ=255)
                                    aCAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTACAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGAt                                                                                                         <  1:5422712/140‑1 (MQ=255)
                                       aCCACTCGGTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATCACGCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTTTTCTGGCTGGATGAAAAGCCGGATAAAAAGAAAAAAGCGATtaa                                                                                                      >  2:2755286/1‑140 (MQ=255)
                                        ccACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTACATCATCGTGCAGGATAAATCTACAAAACGTACACATTCTTTCGATACGTACCCTGTTAATGTGGCAGGATGAACATCCGGTAACAAAGTAAAACGCCATtaat                                                                                                     >  2:325327/1‑140 (MQ=255)
                                         cACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATtaata                                                                                                    <  2:2156700/140‑1 (MQ=255)
                                         cACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATtaata                                                                                                    <  1:4567167/140‑1 (MQ=255)
                                           cTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCTTCTTCGTTGAGGATCACTCGAGAATCCTTAGAATTGCTTTCGCTGCGTGCGCGGTTATTATTGCTGGATGAACCCCCGGATACAAAGAAACAGGCGATTaaaaaa                                                                                                  >  2:2241585/1‑140 (MQ=255)
                                              gTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGa                                                                                                                           >  2:4711981/1‑112 (MQ=255)
                                              gTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAaca                                                                                               <  2:574286/140‑1 (MQ=255)
                                              gTTTAGGTGTGCCGCTGACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAaca                                                                                               <  1:641294/140‑1 (MQ=255)
                                                        gCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAg                                                                                     <  1:1625536/140‑1 (MQ=255)
                                                         ccGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTAGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACACATGATAGc                                                                                    >  2:5461360/1‑140 (MQ=255)
                                                         ccGCTTACTGTCGTTTTTCCTTCTTCGGGCTGGTTGACGCTAGAAACCTTAGACTTTCTTTCGCTGCTTACGCTGTTATTCTGGCTGGATGAACATCCGGATACAAATAAAAAGGCGATTAATAAAACACTCATTATAga                                                                                    >  2:2702501/1‑139 (MQ=255)
                                                         ccGCTTACTGTCGTTTTTCCTTCGTCGTGCTGGCTCACTCTAGAATACGTAGACTTGCTTTCGCGGCTTCCGCTGTTATTCTGGCTGGATGAAAAGCCGGATAAAATGAAAAAGGCGATTAATAAAAAACTCAATATAGc                                                                                    >  2:2482803/1‑140 (MQ=255)
                                                         ccGCTTACTGTCGTTTTTCCTTCGTCGTGCTGGATCACTCTAGAATACTTAGACATGCTTTCCCTGCCACCCCTGTTATTATGGCTGGATGAACAGCCGCATACAAAGAAACAGGCGAAGAAAAAAACACTCATGATacc                                                                                    >  2:5573438/1‑138 (MQ=255)
                                                         ccGCTTACTGTCGTTTTTCCATCTTGGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCCCGGCTTCCGCTGTTATTATGGCTGGCAGAACACCCCGATACAATGAAACAGGGGATTAAGAAAACACGAGTGATAGc                                                                                    >  2:4673648/1‑140 (MQ=255)
                                                         ccGCTTACTGTCGTTTTTACATATTCGTGCTGGCTCACTCAATAAACCATAGACTTGCTTTAGCTGCGTCCCCAGTTATTGTGGCTGGATGAAAACCCCGATAAAATGAAAAAGGCGATTAAAAAAAAACACATGATAGc                                                                                    >  2:1017900/1‑140 (MQ=255)
                                                          cGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCa                                                                                   <  2:295136/140‑1 (MQ=255)
                                                                    cGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGt                                                                         <  1:2530586/140‑1 (MQ=255)
                                                                          tCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAAt                                                                   <  2:2030329/140‑1 (MQ=255)
                                                                            cttcttCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAg                                                                 <  2:3448602/140‑1 (MQ=255)
                                                                                   gTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCa                                                          <  1:4356301/140‑1 (MQ=255)
                                                                                         gATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCAt                                                    >  1:2196012/1‑140 (MQ=255)
                                                                                              ctctAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTgcg                                                            >  1:1002263/1‑127 (MQ=255)
                                                                                               tctAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTcc                                              >  2:288301/1‑140 (MQ=255)
                                                                                                   gAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGAGCATGTCATTCTTCCtttt                                          >  2:3911436/1‑140 (MQ=255)
                                                                                                      tCCTTAGACTTGCTTTCTCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTc                                       <  2:169527/140‑1 (MQ=255)
                                                                                                      tCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTc                                       >  1:4987677/1‑140 (MQ=255)
                                                                                                      tCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTc                                       <  2:1238206/140‑1 (MQ=255)
                                                                                                      tCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTc                                       <  1:4272902/140‑1 (MQ=255)
                                                                                                        cTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTACtttttaaaa                                     >  2:134763/1‑136 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGc                                                                                                                               <  1:4062026/49‑1 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAgt                                    >  1:3894936/1‑139 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAg                                     <  2:5394705/139‑1 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa                                    <  2:3337416/140‑1 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa                                    >  2:2991984/1‑140 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa                                    >  2:728010/1‑140 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa                                    >  2:813261/1‑140 (MQ=255)
                                                                                                         ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa                                    >  2:1439491/1‑140 (MQ=255)
                                                                                                           aGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAAGAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGt                                                                         <  2:654358/101‑1 (MQ=255)
                                                                                                           aGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGAtt                                  <  1:288301/140‑1 (MQ=255)
                                                                                                                 gCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTaa                            >  2:608396/1‑140 (MQ=255)
                                                                                                                 gCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTaa                            >  2:4630352/1‑140 (MQ=255)
                                                                                                                 gCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTaa                            >  2:4562487/1‑140 (MQ=255)
                                                                                                                 gCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTaa                            >  1:3333730/1‑140 (MQ=255)
                                                                                                                  cTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATATCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTAAGATTTAGTAAt                           >  2:3946553/1‑140 (MQ=255)
                                                                                                                  cTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAAt                           >  2:4478912/1‑140 (MQ=255)
                                                                                                                     tCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATgag                        <  1:1914455/140‑1 (MQ=255)
                                                                                                                     tCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATgag                        <  1:4477106/140‑1 (MQ=255)
                                                                                                                      cGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACATGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATgaga                       <  1:1967953/140‑1 (MQ=255)
                                                                                                                      cGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATgaga                       <  2:5776757/140‑1 (MQ=255)
                                                                                                                      cGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATgaga                       <  1:4769729/140‑1 (MQ=255)
                                                                                                                       gctgctTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGaa                      <  2:3547547/140‑1 (MQ=255)
                                                                                                                       gctgctTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCGTCCTTTTTTCAGATTTAGTAATGAGaa                      <  2:4218909/140‑1 (MQ=255)
                                                                                                                           ctTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTACAGATTTAGTAATGAGAATCAt                  <  2:1800194/140‑1 (MQ=255)
                                                                                                                              ccGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTAt               <  1:1791616/140‑1 (MQ=255)
                                                                                                                                 cTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGAATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATcac            >  2:2403921/1‑140 (MQ=255)
                                                                                                                                     tATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGt        >  1:1087641/1‑140 (MQ=255)
                                                                                                                                      aTTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTa       <  1:5727881/140‑1 (MQ=255)
                                                                                                                                      aTTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTa       <  1:1548690/140‑1 (MQ=255)
                                                                                                                                      aTTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTa       <  1:1847615/140‑1 (MQ=255)
                                                                                                                                      aTTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTa       <  1:3600971/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:2641606/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:522010/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:2755286/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:4501125/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:1338841/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:813261/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  2:1092048/140‑1 (MQ=255)
                                                                                                                                        tCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTaaa     <  1:1187777/140‑1 (MQ=255)
                                                                                                                                          tggctggATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAaca   <  1:2256462/140‑1 (MQ=255)
                                                                                                                                          tggctggATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAaca   <  1:3101119/140‑1 (MQ=255)
                                                                                                                                          tggctggATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAaca   <  1:2702501/140‑1 (MQ=255)
                                                                                                                                          tggctggATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAaca   <  1:1473568/140‑1 (MQ=255)
                                                                                                                                          tggctggATGAACAGCCGGATACAATGAAACAGGAGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAaca   <  2:4938937/140‑1 (MQ=255)
                                                                                                                                           ggctggATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAacac  >  1:3976033/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAATGAGAATCATTATCACATGTAAACAC  >  NZ_CP034943/803139‑803417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: