Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 934,369 G→T 7.1% A340S (GCA→TCA)  ssuD → FMNH2‑dependent alkanesulfonate monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943934,3690GT 7.1% 943.2 / 15.9 369A340S (GCA→TCA) ssuDFMNH2‑dependent alkanesulfonate monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (196/134);  new base T (36/2);  total (232/137)
Fisher's exact test for biased strand distribution p-value = 3.92e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

TGGTCAGGGGCGGGGCAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGCCCCCGAAC  >  NZ_CP034943/934232‑934508
                                                                                                                                         |                                                                                                                                           
tGGTCAGGGGCGGGGCAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACAGTTAGAAGAAGCGTATTATTTTGCa                                                                                                                                           <  2:2808780/140‑1 (MQ=255)
 ggTCAGGGGCGGGGCAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGACTCGTTTATATTCTCTGGATATCCACCTTTAGATGAATCGGATTATTTTTCat                                                                                                                                          >  1:5241598/1‑139 (MQ=255)
  gTCAGGGGCGGGGCAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACAGTTAGAAGAAGCGTATTATTTTTCaga                                                                                                                                         >  1:2941785/1‑140 (MQ=255)
  gTCAGGGGCGGGGCAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTGTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCaga                                                                                                                                         >  1:108051/1‑140 (MQ=255)
      ggggcggggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCtt                                                                                                                                     <  2:5615269/140‑1 (MQ=255)
       gggcggggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTCGAAGACTCGGATTATTTTGCAGAGCTtg                                                                                                                                    >  2:3184357/1‑140 (MQ=255)
       gggcggggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTtg                                                                                                                                    >  2:1889547/1‑140 (MQ=255)
            gggcCGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGGTTGTATTCTCTGGATATCCCCATTTATCAGTAGCGGATTATTTTGCAGAGCTTGTGttt                                                                                                                               >  1:3727644/1‑140 (MQ=255)
            gggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATGGTTTATATTCTATTGATATACAAATTTAGAATAAGCGTATTATTTTGCAGAGCTTGTtttt                                                                                                                               >  1:5637892/1‑140 (MQ=255)
            gggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTTTGttt                                                                                                                               >  2:1796135/1‑140 (MQ=255)
            gggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGttt                                                                                                                               >  2:1407556/1‑140 (MQ=255)
            gggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGttt                                                                                                                               >  2:2522897/1‑140 (MQ=255)
            gggcAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTGTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGttt                                                                                                                               >  2:642970/1‑140 (MQ=255)
                 ggAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCa                                                                                                                                           >  1:4579293/1‑123 (MQ=255)
                 ggAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCt                                                                                                                          >  2:1117887/1‑140 (MQ=255)
                 ggAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCt                                                                                                                          >  2:4752272/1‑140 (MQ=255)
                 ggAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATACACATTTAGTAGAAGCGTATTATTTTTCAGAGCTTGTGTTTACGCt                                                                                                                          >  1:3577669/1‑140 (MQ=255)
                 ggAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATACAAATTTAGAAGAAGCGTATTATTTTGCAGAGATTTTGTTTCAGCt                                                                                                                          >  2:2105200/1‑140 (MQ=255)
                 ggAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCAGGATAACCACATTTAGAACAAGCGGATTATTTTGCAGACCTATTGTTTACGCt                                                                                                                          >  1:3811871/1‑140 (MQ=255)
                   aaCAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCAACATTTAGAAGAAGCGTATTATTTTGCAGAGATTGTGTTTCCGCttt                                                                                                                        >  2:875353/1‑140 (MQ=255)
                       gCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGGACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTATAACACCCGTACTATTTTCCAGAGCTTGTGGGTCCGCTATTGcc                                                                                                                    <  2:352646/140‑1 (MQ=255)
                        ctctTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGTGCTTGTGTTTCCGCTTTTGCCg                                                                                                                   >  2:2628339/1‑140 (MQ=255)
                             gTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGa                                                                                                              >  2:2370170/1‑140 (MQ=255)
                             gTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTGTCTATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGGTTGa                                                                                                              >  2:2263859/1‑140 (MQ=255)
                                  tGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATg                                                                                                         >  2:2971347/1‑140 (MQ=255)
                                  tGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATg                                                                                                         >  2:760309/1‑140 (MQ=255)
                                     tCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGc                                                                                                      <  2:1081851/140‑1 (MQ=255)
                                       cGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTTCCGTTTGAAAATGAGCgg                                                                                                    >  2:1023983/1‑140 (MQ=255)
                                       cGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCgg                                                                                                    >  2:3823517/1‑140 (MQ=255)
                                                ttGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTGCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAg                                                                                           >  1:5101756/1‑140 (MQ=255)
                                                      aCCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAg                                                                                     <  1:4231984/140‑1 (MQ=255)
                                                         ggATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAAc                                                                                  <  1:3313591/140‑1 (MQ=255)
                                                           aTAGCTGAGTACCATGAACTCGGCATAGAATCTTTTATATTATCTTGATCTACACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTTTTTCCGCTTTTTCCGTTTGAAAATGAGCGGACGTGAAAGCTTCAGAACaa                                                                                <  2:3757347/140‑1 (MQ=255)
                                                           aTAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACaa                                                                                <  2:381546/140‑1 (MQ=255)
                                                            tAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACaaa                                                                               <  1:2370170/140‑1 (MQ=255)
                                                                  aGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGga                                                                         >  1:4156509/1‑140 (MQ=255)
                                                                    tACCAGGCACTCGGCATAGAATCGTTTATATTCTGTGGATATCCACATTTAGAATAAGCGTATTATTTTTCAGAGCTTTGGTTTCCGCTTTTGCCGGTTGAAAATGAGCGGCCCAGAAAGCTTCAGAACAAAAGGGgaga                                                                       >  1:3109093/1‑140 (MQ=255)
                                                                    tACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGgaga                                                                       >  2:2027455/1‑140 (MQ=255)
                                                                    tACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGACGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGgaga                                                                       >  2:1502910/1‑140 (MQ=255)
                                                                    tACCAGGCACTCGGCATAGAATCGTGTATATTCTCTGGATATCCACATTTAGAAGAAGGGTATTATTTTGCCGTGCTTTTGTTTCCGCTTTTGCCGGTTGAAAATGAGCGGCCGAGAAAGCGTCAGAACAAAACTGgaga                                                                       >  1:3422407/1‑140 (MQ=255)
                                                                     aCCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGATTCAGAACAAAAGGGGAGaa                                                                      >  2:3707285/1‑140 (MQ=255)
                                                                         ggCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTATAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCg                                                                  >  1:3865404/1‑140 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             <  2:5110219/140‑1 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             <  1:1532296/140‑1 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             <  2:5656380/140‑1 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             <  2:2195863/140‑1 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             >  2:482879/1‑140 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTCTCTGGATATACACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             >  1:3822055/1‑140 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATATTATCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             >  2:1754242/1‑140 (MQ=255)
                                                                              tCGGCATAGAATCGTTTATAGTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             <  2:5301854/140‑1 (MQ=255)
                                                                                ggCATAGAATCGTGTATATTCTCTGGATATCCACAGTTAGAAGAAGCGGATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGAAGAGAAACCTTAAGAAAAAAAGGGGAGAAGCAGTCGGGaa                                                           >  2:4640980/1‑140 (MQ=255)
                                                                                        aTCGTGTATATTCTCTGGATATCCACAATTAGAAGAAGGGTATTATTTTTCAGAGCTTGTGTTTACGCTTTTGCCGTTTTAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTAtt                                                   >  1:4787772/1‑140 (MQ=255)
                                                                                           gTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTTCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTGCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTg                                                >  1:5552164/1‑140 (MQ=255)
                                                                                            tttATATTCTCTGGATATCCACATTTAGAAGAAGCGTAATATTTTTCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAAAGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGt                                               >  1:5776897/1‑140 (MQ=255)
                                                                                                   tctcTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAagag                                        >  2:4298591/1‑140 (MQ=255)
                                                                                                     tctGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGaa                                      >  1:2668273/1‑140 (MQ=255)
                                                                                                      ctGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGATTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCTGACGAGAAAGCTTCAGAACACAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGaaa                                     >  2:4548918/1‑140 (MQ=255)
                                                                                                       tGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGaaaa                                    >  1:756799/1‑140 (MQ=255)
                                                                                                            atCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCg                               <  1:142672/140‑1 (MQ=255)
                                                                                                            atCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCg                               <  2:4938275/140‑1 (MQ=255)
                                                                                                            atCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCg                               <  2:1281586/140‑1 (MQ=255)
                                                                                                            atCCACATGTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGGTTCCGCTATTGCGGTTTGATAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATATTGTCAAAGAGAACAATGCg                               <  1:1657092/140‑1 (MQ=255)
                                                                                                             tCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGt                              <  1:482879/140‑1 (MQ=255)
                                                                                                             tCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGt                              <  1:489378/140‑1 (MQ=255)
                                                                                                             tCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGt                              <  2:5270831/140‑1 (MQ=255)
                                                                                                             tCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGt                              <  1:920047/140‑1 (MQ=255)
                                                                                                              ccACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAATAGGGGAGAAGCCGTCGGGACCACTTATTTTGTCAAAGAGAAAAATGCGTc                             >  2:3594929/1‑140 (MQ=255)
                                                                                                               cacaTTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCa                            >  1:399137/1‑140 (MQ=255)
                                                                                                                 caTTTAGAAGAAGCTTATTATTTTGCCGAGCTTGTGTTTCCTCTTTTGCCGTTTGAATATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTGGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGt                          <  2:162652/140‑1 (MQ=255)
                                                                                                                 caTTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGt                          <  1:1192359/140‑1 (MQ=255)
                                                                                                                 caTTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGt                          >  1:2918537/1‑140 (MQ=255)
                                                                                                                 caTTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGt                          <  1:2273611/140‑1 (MQ=255)
                                                                                                                   tttAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGttt                        <  1:4278029/140‑1 (MQ=255)
                                                                                                                   tttAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGttt                        <  1:2543013/140‑1 (MQ=255)
                                                                                                                        aagaagCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGttt                        <  2:2138479/135‑1 (MQ=255)
                                                                                                                        aagaagCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTa                   >  1:5044160/1‑140 (MQ=255)
                                                                                                                           aagCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGACCAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTaaaa                <  2:2912152/140‑1 (MQ=255)
                                                                                                                           aagCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGTGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTaaaa                >  2:4011262/1‑140 (MQ=255)
                                                                                                                            agCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCggg                                                             >  1:2146022/1‑94 (MQ=255)
                                                                                                                            agCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAac               >  2:58747/1‑140 (MQ=255)
                                                                                                                            agCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTGCGGACAAAATGGGAGGAGCCCTCGGGAACAAATATTTTTTGCAAGATAAAAAATCGTCAGTTTAAGTAAAaca              >  1:5700823/1‑140 (MQ=255)
                                                                                                                             gCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGt                              <  2:4219197/124‑1 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             <  2:4156509/140‑1 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             >  2:4409367/1‑140 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             <  1:247343/140‑1 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             >  1:4070533/1‑140 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             >  1:2513824/1‑140 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             >  1:5463523/1‑140 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             <  1:5351777/140‑1 (MQ=255)
                                                                                                                              cGTATTATTTTGCAGAGCTTGTGTGTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAacac             <  2:5710118/140‑1 (MQ=255)
                                                                                                                                tattatTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAg           >  1:1826452/1‑140 (MQ=255)
                                                                                                                                tattatTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCGTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAg           >  1:284696/1‑140 (MQ=255)
                                                                                                                                   tattTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGccc        <  1:4409367/140‑1 (MQ=255)
                                                                                                                                    attTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGcccc       <  2:1940997/140‑1 (MQ=255)
                                                                                                                                     ttttGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGccccc      >  2:3395525/1‑140 (MQ=255)
                                                                                                                                        tGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAATTGAGCGGACGAGAAAGCTTCAGACCAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACAAAGCCCCCGaa   >  1:1937360/1‑140 (MQ=255)
                                                                                                                                        tGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGCCCCCGaa   <  2:5357400/140‑1 (MQ=255)
                                                                                                                                        tGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGCCCCCGaa   <  2:2671851/140‑1 (MQ=255)
                                                                                                                                        tGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGCCCCCGaa   <  1:2120440/140‑1 (MQ=255)
                                                                                                                                        tGCAGAGCTTGTGTTCCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGCCCCCGaa   >  1:1595424/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGGTCAGGGGCGGGGCAGGAACAGCTCTTGTCGGTGATCCGAAGACTATTGCCGACCGGATAGCGGAGTACCAGGCACTCGGCATAGAATCGTTTATATTCTCTGGATATCCACATTTAGAAGAAGCGTATTATTTTGCAGAGCTTGTGTTTCCGCTTTTGCCGTTTGAAAATGAGCGGACGAGAAAGCTTCAGAACAAAAGGGGAGAAGCCGTCGGGAACACTTATTTTGTCAAAGAGAAAAATGCGTCAGTTTAAGTAAAACACAGCCCCCGAAC  >  NZ_CP034943/934232‑934508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: