Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,151,967 T→G 12.5% K79T (AAA→ACA)  EO946_RS05920 ← GNAT family N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,151,9670TG12.5% 455.8 / 11.0 212K79T (AAA→ACA) EO946_RS05920GNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (79/96);  new base G (35/2);  total (114/98)
Fisher's exact test for biased strand distribution p-value = 7.55e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTG  >  NZ_CP034943/1151828‑1152106
                                                                                                                                           |                                                                                                                                            
tCGTACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTGGGCTGAGACACGCGTTCACCATCAGCCGTCCGAGACCGAGCCGtt                                                                                                                                              <  2:5017413/140‑1 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCCCACCTTGTTTTGTGGCGCAAAGCCTGATAGCGGTGCGTTCATCCGGTTGGCTGTTACACGCGTGCACCATCCGCCGTCCGAGACCGAGCGGtt                                                                                                                                              >  2:2862380/1‑140 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAAGCTGCTTTTGTGGCGCAACGCCTGATAGCGGTGCTTTCATCCGTTGGGCTGATACACGCGTTCACCTTCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                              >  2:3417446/1‑140 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                              <  2:2756421/140‑1 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                              <  2:5188195/140‑1 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                              >  1:1994421/1‑140 (MQ=255)
ccGGACTGAACCCGCTGTTTGAAAACCCGCGTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                              <  2:4394140/139‑1 (MQ=255)
  ggACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTg                                                                                                                                            >  1:3153938/1‑140 (MQ=255)
    aCTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTa                                                                                                                                          <  1:1205055/140‑1 (MQ=255)
    aCTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTa                                                                                                                                          <  2:1021167/140‑1 (MQ=255)
    aCTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTa                                                                                                                                          <  2:693781/140‑1 (MQ=255)
    aCTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTa                                                                                                                                          <  2:834868/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                      >  2:5663334/1‑140 (MQ=255)
          cccGCTGTTTGAAAACCCGCTTTCTCATAACGCGGCAAACCTTCTGTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCCTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTAttt                                                                                                                                    >  1:3841575/1‑140 (MQ=255)
          cccGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATCCACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTGttt                                                                                                                                    >  1:136011/1‑140 (MQ=255)
          cccGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCgtt                                                                                                                                    >  1:864906/1‑140 (MQ=255)
            cGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGt                                                                                                                                  <  1:2902897/140‑1 (MQ=255)
                 tttGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACg                                                                                                                             <  1:5791508/140‑1 (MQ=255)
                  ttGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGCCCGAGCCGTGTGTATTCTTTGTCTACGa                                                                                                                            >  2:1773275/1‑140 (MQ=255)
                  ttGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGa                                                                                                                            >  2:4008168/1‑140 (MQ=255)
                  ttGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGa                                                                                                                            >  2:1384330/1‑140 (MQ=255)
                      aaaCCCGCTTTCTCCTAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                        <  1:276429/140‑1 (MQ=255)
                      aaaCCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                        <  1:2322667/140‑1 (MQ=255)
                      aaaCCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                        <  2:5132621/140‑1 (MQ=255)
                         cccGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAg                                                                                                                     >  2:3968017/1‑140 (MQ=255)
                         cccGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTATTTTTCTACGATGACGAg                                                                                                                     >  1:1176499/1‑140 (MQ=255)
                         cccGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTAGTCGTTGTCAACGAAGACGAg                                                                                                                     >  2:5602909/1‑140 (MQ=255)
                          ccGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGt                                                                                                                    <  1:4275996/140‑1 (MQ=255)
                           cGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTc                                                                                                                   <  2:2348647/140‑1 (MQ=255)
                           cGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTc                                                                                                                   >  2:166197/1‑140 (MQ=255)
                           cGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTc                                                                                                                   <  2:1247204/140‑1 (MQ=255)
                           cGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGGTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTGTGTCTACGATGACGAGTc                                                                                                                   >  2:4960553/1‑140 (MQ=255)
                              tttCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGa                                                                                                                <  1:5588517/140‑1 (MQ=255)
                              tttCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGa                                                                                                                <  2:4614423/140‑1 (MQ=255)
                               ttCTCCTAAAGCGGCAAACCATCATTTGTGGCGCACAGCCTGAGAGCGGTGCTTACATCCGTTTGGCTGATACCCGCGTTCACCATCCGCCGTCCGAGACCGACCCGATTGTATTCTTTGTCAACGATGACGAGTCCgct                                                                                                               <  2:5645915/140‑3 (MQ=255)
                               ttCTCATACAGCGGCAAACCTTCTTTGGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCGGATACCCGCGTTCACCAGCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCGACGATGACGAGTCCGAt                                                                                                               <  1:141337/140‑1 (MQ=255)
                               gtctcATACAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGAGAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGAGGACGAGTCCGAt                                                                                                               <  2:3679732/139‑1 (MQ=255)
                                    aTAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                              <  2:3385275/104‑1 (MQ=255)
                                        aGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAg                                                                                                      >  2:4865510/1‑140 (MQ=255)
                                                       tttGTGGCGCAAAGCGTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCaaacaaaaa                                                                                      <  1:3704264/140‑1 (MQ=255)
                                                       tttGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCaaacaaaaa                                                                                      <  1:379437/140‑1 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCAACCATGAAGAGTCCGATAGAGGCAAGAACGCCcaacaaaaaacagc                                                                                 >  1:5217711/1‑135 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATGACGAGTCCGATAGATGAAATACCGCCAAAAAAAAACAAGc                                                                                 >  2:2447293/1‑140 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATGACGACTACGATAGAGGCAATACGGCCaaacaaaaacaacc                                                                                 >  2:3878058/1‑138 (MQ=255)
                                                                 aaaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTACGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATg                                                                            <  2:5438075/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                           <  1:1309372/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                           <  1:5668067/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                           <  1:166197/140‑1 (MQ=255)
                                                                  aaGCCTGAGAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                           <  2:4357054/140‑1 (MQ=255)
                                                                   aGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCa                                                                          <  1:3034352/140‑1 (MQ=255)
                                                                       tGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGaa                                                                      <  1:2562358/140‑1 (MQ=255)
                                                                       tGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGaa                                                                      <  1:3380111/140‑1 (MQ=255)
                                                                        gATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTGTTTTTATACGAATAACAGTACGATAGACGCAAGACGGCCAAAAAAAAAAAAGCAGGTGCAGGaaa                                                                     >  2:1524023/1‑140 (MQ=255)
                                                                        gATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTATTTGTGTACGATGACGAGGCCGATAGAGGCAACACGGCCCAACAAAAAAAACCCGAATCAGGaaa                                                                     >  1:3069668/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGAAGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAACCCGATGCAGGAAATAATGtc                                                              >  2:222749/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTAAGATGACGAGTGCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGtc                                                              >  2:4264227/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGGCTAACATGCCCTGTGCGCGATAGGCCACACGGCCAAACAAAAACAAGCCGATGCCGGCAATAAAGtc                                                              >  2:5760770/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTTTTCTTTTTATACCGTGAAGACTCCGCATGAGGGAACACAACCACACAAAAAAAACACGATGAAGGAGAAAATGta                                                              >  1:1583914/1‑139 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTGTTTGTCTAAGGTTACCAGTCCGATAGTGGGAACACGGCCAAACACAAAAAATCCGATGCAGGGAAAAAAGta                                                              >  1:5417629/1‑139 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATACCCCGTCCGAGACCGAGCCGTTAGTATTTTTTGTCTACGATGCCGAGTCCGAAAGAGGCAAAACCGCCCAACAAAAAAAAGCCGCTGCAGGAAAATATGtg                                                              >  2:2032474/1‑139 (MQ=255)
                                                                                  cTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGACGAAGCCGGACATAAAGTCTcc                                                           >  2:3704922/1‑140 (MQ=255)
                                                                                     tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                        <  1:4695536/140‑1 (MQ=255)
                                                                                     tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTTTTTTTTTTTTGTACCATTACGAGTGCGCTAGAGGCAACACCGGCAAAAAAAAAAAATACCATGCAGGGAAAAATTTCTCcgcg                                                        >  1:464465/1‑140 (MQ=255)
                                                                                       aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTGTTTGTCTACGATGACGGAGCGGCTTGTGGCAAGACGGGGAATCGCTAAGAAGCCGGTGGCGGTCATTATGTGTCCGCggc                                                      >  2:3053632/1‑139 (MQ=255)
                                                                                       aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATTACCAGTCCGATCTAGGCAAGACGGGCAAACAAAAACAAGCCGATGCAGGAAATAATGTCGCCGCGGa                                                      >  2:1354733/1‑140 (MQ=255)
                                                                                       aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAAACGGCCAAACAAAAAAAAGCCGATGCACGTAAAAATTTCTACGCggc                                                      >  2:3649894/1‑139 (MQ=255)
                                                                                       aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTAAGATGACGAGTCCGATATTGGGAAGACGGCCAAACAAAAAAAATCCGATGCAGGAAATAATGTCTCCGCGGa                                                      >  2:2524788/1‑140 (MQ=255)
                                                                                        tCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGAc                                                     <  1:2501030/140‑1 (MQ=255)
                                                                                            tttGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACAttt                                                 >  2:4880145/1‑140 (MQ=255)
                                                                                            tttGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAAAAAAAACAAGCCGCTGCAGGAAATAATGTCTCCGCGGACCttt                                                 >  2:4569965/1‑140 (MQ=255)
                                                                                              tGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTATTTGTCTACGATGACGAGGCCGATAGAGGCAAGACGGCCAAACAAAAACAATCCGATGCAGGAAATAAAGTCTCCGCGGACAttttt                                               >  2:2819668/1‑139 (MQ=255)
                                                                                               ggCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCGTTTTGTACCATGACGAGTGCGCTAGAGGCAAGCCCGCCCAAACAAAAAAACCCCATGGAGGAAAAAAAGTGTCCGCGGGCATTTTgt                                              >  1:4832969/1‑139 (MQ=255)
                                                                                                 cTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGCATTCTTTGTCTACGATTACGAGTCCGATAGAGGCAAGACGGCCCAACAAAAACAAACCGATGCACGAAATAATGTCTCCCCGGACATTTTtata                                            >  2:3501728/1‑140 (MQ=255)
                                                                                                          gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTAAGATTACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAAGCCGATGCAGGAAATAATGTCTACGCGGACCTTTTTATAACCGaaaaa                                   >  2:3114127/1‑140 (MQ=255)
                                                                                                           cgTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTTTTCTTTTTAGACGATGAGGGGTCCGATAGGCGCAACACGGCCAAACCAAAAAAAGCCGAAGGCGGAAATAATGTCTGCGCGGACAATTTTTTAAACGAAAAAg                                  >  1:2438154/1‑140 (MQ=255)
                                                                                                             ttCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGGGGACATTTTGATAACCGAAAAAgcg                                <  2:383082/140‑1 (MQ=255)
                                                                                                              tCACCATCCGCCGTCCGAGACCGAGCCTTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGTAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcgc                               <  1:221792/140‑1 (MQ=255)
                                                                                                              tCACCATCCGCCGTCCGAGACCGAGCCTTTTGTATGCTTGGTCTACGATGACGAGGCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCGCCGCGGACATTTTGATACCCGAAAAAgcgc                               <  1:4428731/140‑1 (MQ=255)
                                                                                                              tCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcgc                               <  1:820073/140‑1 (MQ=255)
                                                                                                               cACCATCCGCCGTCCGAGACCGAGCCGTTTGTCTTATTTGTCTACGATGACGAGTCCGATAGAGGCAAGAAGGCCCAACAAAAAAAATCCGATGGAGGAAATAATTTCTCCCCGGCCAATTTGATAACCGCAAAACCGcc                              >  2:932288/1‑140 (MQ=255)
                                                                                                               cACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTGTTTGTCTACGATGACGAGTGCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCCGGAAATAATGTCTCCGCGGACATTTTTTTAACCGAAAAAGCGcc                              >  2:38445/1‑140 (MQ=255)
                                                                                                                   aTCCGCCGTCCTAGACCGCGACGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCCAGACGGCCAAACAAAAACAAGCCTATGCAGGAAATATTGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTg                          <  1:1936771/140‑1 (MQ=255)
                                                                                                                   aTCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTg                          <  1:4083/140‑1 (MQ=255)
                                                                                                                        ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                     <  2:3774457/140‑1 (MQ=255)
                                                                                                                            ccGATACCGCGCCGTGTGAATTATCTCTCTACGATGACGAGTCCGATACAGGCAAGACGGCCAAACAAAAACAAGCCTATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCTAAAAAGCGCCCCTGTTGGACAAg                 <  1:3259930/140‑1 (MQ=255)
                                                                                                                            ccGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAg                 <  2:725735/140‑1 (MQ=255)
                                                                                                                            ccGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAg                 <  2:1503837/140‑1 (MQ=255)
                                                                                                                                gaCCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAAt             <  1:3403819/140‑1 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTGTTTGTCTACGATGACGAGTCCGAAAGAGGCAAGACGGCCCAACAAAAACAAGCCCATGCAGGAAAAAATGTCTCCGCGGACAATTTGATAACCGAAAAAGCGCCCCGGTTGGACAAGCCATGCCAg        >  2:3026099/1‑140 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTCTTTGTCTACGATTAAGAGTCCGATAGAGGCAAGACGGCCAAAAAAAAACAAGCCGATGCAGGTAATAATGTGTGCGCGGACATTTTGATAACCGAAAAAGCGCCCCGGTTGGAGAAGCAATggccg        >  2:1940746/1‑136 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTCTTTGTCTACGATGACGAGTGCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGGACGAAAAAATGTCTCCGCGGTCATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGcccg        >  2:5523517/1‑138 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAAAAATGTGTCCGCGGACATTTTTATAAACGAAAAAGCGCCCCTGTTTGACAAGCAATGCCAg        >  2:4543681/1‑140 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTCTTTGTCTAAGATGACCAGTCCGATTGAGGGAAGACGGCCAAACAAAAACAAGCCCTTGCAGGAAATAATGTCTCCGCGGAAATTTTTATAACCGAAAAAGAGCCCCTGTTTGACAAGCAATGCCAg        >  2:5456603/1‑140 (MQ=255)
                                                                                                                                     aGCCGTTTGGATTCTTTGTCTACGATGACGAGTCCGAAAGAGGCAAGACGGCCAAACAAAAACAAGCCGAAGCAGGAAATAAAGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCAGTTGGACAAGCAATGCCac        >  2:1422771/1‑139 (MQ=255)
                                                                                                                                     aGCCGTGTGTATTGTTTGTGTAAGATTACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAAACCCAAAACCGCCCCGGTTGGACCAGCAATGcccg        >  2:1800090/1‑138 (MQ=255)
                                                                                                                                     aGCCGTGTGTATTCTTTTTCTACGATGACGAGTCCGATAGAGGCAAGACGGGCCAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAg        >  2:2045823/1‑140 (MQ=255)
                                                                                                                                     aGCCGTGTGTATTCTTTTTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAg        >  2:4150871/1‑140 (MQ=255)
                                                                                                                                          tttGTATTCTTTTTCTACGATTACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAATCCGATGCAGGAAAAAATGTCTCCGCGGACATTTTGATAAACGAAAAAGCGCCCCTGTTTGACAAACAATGCCAGCTCtt   >  2:5728590/1‑140 (MQ=255)
                                                                                                                                           ttGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTg  <  2:2317488/140‑1 (MQ=255)
                                                                                                                                           ttGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTg  <  2:1306128/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTG  >  NZ_CP034943/1151828‑1152106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: