Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,263,936 G→T 7.7% T83K (ACA→AAA)  fetB ← iron export ABC transporter permease subunit FetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,263,9360GT 7.7% 1056.1 / 18.2 379T83K (ACA→AAA) fetBiron export ABC transporter permease subunit FetB
Reads supporting (aligned to +/- strand):  ref base G (165/170);  new base T (2/42);  total (167/212)
Fisher's exact test for biased strand distribution p-value = 9.22e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 381 total aligned reads displayed.

TTTCCGATGACCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCG  >  NZ_CP034943/1263801‑1264075
                                                                                                                                       |                                                                                                                                           
tttCCGATGACCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTAtt                                                                                                                                         >  1:194293/1‑140 (MQ=255)
       tGACCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTtttcttt                                                                                                                                  <  2:1288577/140‑1 (MQ=255)
       tGACCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTtttcttt                                                                                                                                  <  2:246190/140‑1 (MQ=255)
         aCCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTTCACTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCg                                                                                                                                <  2:3413267/140‑1 (MQ=255)
         aCCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCCAACCGAACCGATTGTATTTTTCTTTCg                                                                                                                                <  1:861032/140‑1 (MQ=255)
             ttCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTg                                                                                                                            >  2:4827143/1‑140 (MQ=255)
                     aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtt                                                                                                                    >  1:4150675/1‑140 (MQ=255)
                      tCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATCTTTCTTTCGTTTGATAACAttt                                                                                                                   <  1:3693461/140‑1 (MQ=255)
                       cGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtttt                                                                                                                  >  1:3225981/1‑140 (MQ=255)
                       cGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtttt                                                                                                                  >  1:314005/1‑140 (MQ=255)
                          aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATa                                                                                                                         >  2:5023895/1‑130 (MQ=255)
                               cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                          >  1:2564778/1‑140 (MQ=255)
                               cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                          >  1:2222521/1‑140 (MQ=255)
                               cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                          >  1:3268614/1‑140 (MQ=255)
                               cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                          >  1:3638169/1‑140 (MQ=255)
                                atatCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTa                                                                                                         <  1:3790619/140‑1 (MQ=255)
                                atatCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTa                                                                                                         <  2:789109/140‑1 (MQ=255)
                                atatCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTa                                                                                                         <  2:3549710/140‑1 (MQ=255)
                                   tCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                      <  1:1726061/140‑1 (MQ=255)
                                    cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                     >  2:4122917/1‑140 (MQ=255)
                                     gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                    <  1:2759466/140‑1 (MQ=255)
                                     gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                    <  1:5256838/140‑1 (MQ=255)
                                     gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                    <  2:4701887/140‑1 (MQ=255)
                                      ggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCa                                                                                                   <  1:5499814/140‑1 (MQ=255)
                                       gCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCaa                                                                                                  >  1:4316346/1‑140 (MQ=255)
                                        ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                 >  1:5797710/1‑140 (MQ=255)
                                        ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                 <  1:4557019/140‑1 (MQ=255)
                                            aaGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTa                                                                                             <  2:2274496/140‑1 (MQ=255)
                                                  gatgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCa                                                                                       <  2:1657213/140‑1 (MQ=255)
                                                  gatgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCa                                                                                       <  2:3087964/140‑1 (MQ=255)
                                                  gatgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCa                                                                                       <  2:526153/140‑1 (MQ=255)
                                                   atgatATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCAc                                                                                      >  1:3956921/1‑140 (MQ=255)
                                                          gAAGCGACCCCAGAATTCCCTGCGTCACAAGTTCTACAATCGCTAACGCGGCGAACACCCTACACAACGCACGGATTGTATTCTTCTTGCCTTTGATAAAATTTTGCGCGGCTACTGCCAACCTTAACAGCACCATCAGc                                                                               <  2:4392246/140‑1 (MQ=255)
                                                          gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGTCAACATTAACAGCACCATCAGc                                                                               <  2:4465747/140‑1 (MQ=255)
                                                          gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                               <  1:963883/140‑1 (MQ=255)
                                                          gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                               <  1:2299568/140‑1 (MQ=255)
                                                          gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                               <  1:1806513/140‑1 (MQ=255)
                                                          gAAGAGACAGCAGAAGTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                               <  1:400754/140‑1 (MQ=255)
                                                            agagACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCaa                                                                             >  1:3902762/1‑140 (MQ=255)
                                                               gaCAGCAGAAGTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAACCGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGAt                                                                          <  1:5657846/140‑1 (MQ=255)
                                                                aaagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                         >  2:2534753/3‑140 (MQ=255)
                                                                aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                         >  1:1140258/1‑140 (MQ=255)
                                                                aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                         >  2:3693002/1‑140 (MQ=255)
                                                                aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                         >  1:506848/1‑140 (MQ=255)
                                                                aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                         >  2:2830284/1‑140 (MQ=255)
                                                                aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                         >  1:3749898/1‑140 (MQ=255)
                                                                 cagcggAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                        >  1:1040039/1‑140 (MQ=255)
                                                                 cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                        >  1:4521071/1‑140 (MQ=255)
                                                                 cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                        >  1:721188/1‑140 (MQ=255)
                                                                  agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCATTCGTTTGATAACATTTTGAGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaa                                                                       >  1:5139401/1‑140 (MQ=255)
                                                                   gcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAACCGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaa                                                                      <  2:5208682/140‑1 (MQ=255)
                                                                   gcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaa                                                                      <  1:4447140/140‑1 (MQ=255)
                                                                   gcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaa                                                                      <  2:3990793/140‑1 (MQ=255)
                                                                   gcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaa                                                                      <  1:654219/140‑1 (MQ=255)
                                                                   gcagAAGTCCCTGAGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaa                                                                      <  2:3960832/140‑1 (MQ=255)
                                                                       aaTTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAg                                                                  >  1:4651450/1‑140 (MQ=255)
                                                                              tccGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCTATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCACCATTAACAGCACCATAAGCAAGATAAAAACAGCAGGGtc                                                           >  1:3855716/3‑140 (MQ=255)
                                                                              tGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGAGTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtc                                                           >  1:4189698/1‑140 (MQ=255)
                                                                                cGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAtt                                                                                              <  2:507222/103‑1 (MQ=255)
                                                                                    acaATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCcgcg                                                     <  1:2128645/140‑1 (MQ=255)
                                                                                                aTCGCTAACGGGGCGAACCCCCTCCAAAACTAACCTTTTTTATTTTTATTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGTTGGTCTCCGCGAAAAATAAGCGa                                         <  1:1441992/140‑1 (MQ=255)
                                                                                                aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCgc                                         >  2:1521773/1‑139 (MQ=255)
                                                                                                aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                         >  2:1895214/1‑140 (MQ=255)
                                                                                                aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                         >  1:2455124/1‑140 (MQ=255)
                                                                                                aTCGCTAACGCGGCGAACACCCTCCAAAACCAACCGATTGTATTTTTCTTTCGTTTGATAACATTGTGCGCTGCTACGGCCAACATTAACAGCACCCTCCGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                         <  2:5371324/140‑1 (MQ=255)
                                                                                                aTCCCTAACGCGGCTACCCCCCTCAAAAACGAACCGATTTTATTTTTCTTTCTTTTTATAACATTTTGCGCTGCTACTCCCAACATTAACAGCACAACCAGCAAGATAAAAACAGGCGGGTCTCCGCGAAAAATACGCGa                                         <  1:4880579/140‑1 (MQ=255)
                                                                                                aTCCCTAACGCGGCGAACACCCTCAAAAACTAACCGATTGTATTTTTCTTTCTTTTTATAACATTTTGGGCGCCTACGGCAAAAATTAACAGCACAATCACCAAGATAAAAACAGGATGGTATCCGCGAAAAATAAGCGa                                         <  2:2148269/140‑1 (MQ=255)
                                                                                                  cGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACa                                       <  2:3749898/140‑1 (MQ=255)
                                                                                                    cTAACGCGGCGAACACCCACCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCTAACATTAACAGCACCATCAGCAATATAAAAACAGGATGGTCTCCGCGAAAAGTAAGCGTCAGt                                     >  1:4208210/1‑140 (MQ=255)
                                                                                                           gcacaaCACCCACCAAAACCACCCTTTTTTTTTTTTCTTTCTTTTGAAAAAATTTTGCCGTGATACTCCCAAAATTAACCCAACAATCAGAAACAAAAAAACCGGTTGGTCTCCGCTAAAAATAAGCGACAGTACATAAc                              <  1:3369717/136‑1 (MQ=255)
                                                                                                             ctaaCACCCACCAAAACCAACCTTTTGTTTTTTTTTTTGTTTTGATAAATTTTTGCGCGGCTACTGCAAAAATTAACACCACCATCAGCAATATAAAAACAGGTTGGTAGCCGCTAAAAATAAGCGACAGTACATAACCg                            <  1:1307898/138‑1 (MQ=255)
                                                                                                                   cccTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGAACCTACAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                      <  2:2222521/140‑1 (MQ=255)
                                                                                                                   cccTCCAAAACGAACCGATTGTATTTTTATTTCTTTTTATAACATTTTGCGCGGCTACTGCAAAAATTAACAGCACCATCAGCAAGATAAAAACAGGAGGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                      <  2:314005/140‑1 (MQ=255)
                                                                                                                   cccTCAAACACGACCCTTTTGTTTTTTTGTTTGGTTTTATAACATTTGGCGCTGCTACGGCAAAATTTAACCGCACCATCAGCACTATAAAAACAGTATGGTCTCCGCCAAAAATAAGCGACAGTACATAACCGATGATa                      <  1:1539328/140‑1 (MQ=255)
                                                                                                                   cccTCAAAACCGAACCGTTTTTATTTTTCTTTCGTTTGTTAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGAAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                      <  2:3268614/140‑1 (MQ=255)
                                                                                                                   cccACCAAAACCAACCGTTTTTATTTTTGTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                      <  2:3225981/140‑1 (MQ=255)
                                                                                                                   ccacccAAACCCACCCTATTTTATTTTTCTTTCTTTTTATAAAATTTTGCGCTGCTACTCCAAACATTAACAGCACAAACAGCAAGATAAAAACAGGATGGTCTCCGCCAAAAAAAAGCGACAGTACATAACCGATGATa                      <  1:1521773/136‑1 (MQ=255)
                                                                                                                   ccacaaaaaaCCAACGGATTTTATTTTTCTTTCTTTTGATAACATTTTGCGCTGCTACTGCAAACATAAACAGCACCAACAGCATTATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATa                      <  1:1610172/134‑1 (MQ=255)
                                                                                                                     ccccAAAACCAACCTTTTTTATTTTTCTTTCGTTTGATAACATTTGGCGCTGCTACTCCCAAAATAACCACCACCAACACCAAGAAAAAAACAGGATGGACTCCGCGAAAAATACGCGACAGTACATAACCGATGATAag                    <  1:2915466/138‑1 (MQ=255)
                                                                                                                     caccAAAACGAACCTTTTTTATTTTTTTTTCTTTTTATAACTTTTTGCGCTGATACTGCAAAAATTAACAGAACAATCACAAATATAAAAACTGGTTGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAag                    <  1:2539508/138‑1 (MQ=255)
                                                                                                                     cTCCAAAACTAACCTTTTTTATTTTTCTTTCTTTTTATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAag                    <  2:1960806/140‑1 (MQ=255)
                                                                                                                      tcaaaaaAGAACCGATTTTATTTTTCTGTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACACCAACATCAGCAATATAAAAAAAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                   <  2:3855716/137‑1 (MQ=255)
                                                                                                                      tCCAAACCGAACCGATTTTATTTTTCTTTCTTTTGATAACATTTTGCGCGGCTACTGCCAACATTAACAGCACCATCACCAAGATAAAAACCGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                   <  2:3638169/140‑1 (MQ=255)
                                                                                                                      tCCAAAACGAACCGATTTTATTTTTGTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTACCAGCACAAACAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                   <  2:4194013/140‑1 (MQ=255)
                                                                                                                      tCCAAAACGAACCGATTGTATTTTTCTTTCGATTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCAGCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATaatc                   >  2:2854902/1‑138 (MQ=255)
                                                                                                                      acaaaaaCGAACCGTTTTTATTTTAGTTTCTTTTTATAAAATTTTGCGCTGATACTGCCAACTTTAACACCACCATCAGCAAGATAAAAACTGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                   <  1:2830284/137‑1 (MQ=255)
                                                                                                                       ccAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagca                  >  1:2051886/1‑140 (MQ=255)
                                                                                                                        cAAACCCAACGGATTGTTTTTTTCTTTCTTTTTATAACTTTTTGCGCTGCTAGTGCAAAAATTACACGCACCATCACAATTATAAAAACCGGTTGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAagcag                 <  1:3039843/140‑1 (MQ=255)
                                                                                                                        cAAAACGAACAGTTTTTATTTTTCTGTCGTTTTATAACATTGTGCGCTGCTACTCCCAACATTAACACCACCATAAGCATGATAAAAACTGTTTGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagcag                 <  1:3521190/140‑1 (MQ=255)
                                                                                                                               aaCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAcc          >  1:2688861/1‑140 (MQ=255)
                                                                                                                               aaCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAcc          >  1:4776555/1‑140 (MQ=255)
                                                                                                                                cccGATTGTATTTTTCTTTCGTTTCATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg         <  2:1080488/139‑1 (MQ=255)
                                                                                                                                aCCGATTGTTTTTTTCTTTCTTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCAACTGCAATATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg         <  2:4323894/140‑1 (MQ=255)
                                                                                                                                aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg         >  2:5582027/1‑140 (MQ=255)
                                                                                                                                aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTCCCAACATTAACAGCACCATCATCAAGATAAAAACAGTATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg         >  1:497769/1‑140 (MQ=255)
                                                                                                                                aCCGATTGTATTTTTCTTTCGTTTGATAAAATTTTGCGCTGCTACTGCCAAAATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCTAAAAATAAGAGACAGTACATAACCGATGATAAGCAGCTGTACCg         >  2:5428651/1‑140 (MQ=255)
                                                                                                                                     ttGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTCCCAACATTAACAGCACCATCAGCAATATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGcc    <  2:1451469/140‑1 (MQ=255)
                                                                                                                                     ttGTATTTTTCTGTCGTTTGATAACATTTTGCGCGGCTACTGCCAACATTAACAGCACCAACAGCAAGATAAAAACAGGAGGGTCTCCGCGAAAAATACGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGcc    <  2:535265/140‑1 (MQ=255)
                                                                                                                                       gTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCAACAGCAAGATAAAAACAGGAGGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGGACCGCTGCCCg  <  2:2740623/140‑1 (MQ=255)
                                                                                                                                       gTATTTTTCTTTCGTTTGATAACATTGTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACCGGAGGGTCTCCGCGAAAAATACGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCg  <  2:580796/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
TTTCCGATGACCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCG  >  NZ_CP034943/1263801‑1264075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: