BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,488,182	G→T	8.1%	A575S (GCA→TCA)	ade →	adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,488,182	0	G	T	8.1%	511.5 / 7.9	200	A575S (GCA→TCA)	ade	adenine deaminase
Reads supporting (aligned to +/- strand): ref base G (77/99); new base T (18/6); total (95/105)
Fisher's exact test for biased strand distribution p-value = 4.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGA  >  NZ_CP034943/1488046‑1488320

tGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATattgcat                                                                                                                                         >  2:226815/1‑140 (MQ=255)

tGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATattgcat                                                                                                                                         <  2:5150305/140‑1 (MQ=255)

  aTAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCTGTCATTCCTGACATCAAAATTAAGACAACAGGATTATTTTATGTAAAATCAATTAACCATatttcattg                                                                                                                                       >  2:4376744/1‑140 (MQ=255)

    aaaTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATAttgcattgca                                                                                                                                     <  2:5552665/140‑1 (MQ=255)

      aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTACTGACATAAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTAAGCATATTGCATTGCAAt                                                                                                                                   >  2:1596798/1‑140 (MQ=255)

        tGTTATTAGTCGGTTTCACAGGCGGGTTTACTCCCTTTTTGACGCTGTCGTTTTTATCTTTTCCGGGAATTCCGGACAACAAAATGACGACAAAAGGCTTATTTTTGTTAAAACAAATTAAGCATAATGTATTGCAaaaa                                                                                                                                 >  1:613038/1‑140 (MQ=255)

        tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATAATTTCAGCATATTTCATTGCAATaa                                                                                                                                 >  2:4650097/1‑140 (MQ=255)

        tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTACTGACATCAAAATGACGACAAAAGGATTATTTGATGTAAAATAATTTCAGCAAATTTAATTTAAATaa                                                                                                                                 >  1:393030/1‑140 (MQ=255)

                gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGATGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                         <  2:4721203/140‑1 (MQ=255)

                 tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                        <  1:679172/140‑1 (MQ=255)

                 tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                        <  2:4123078/140‑1 (MQ=255)

                 tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                        <  1:869109/140‑1 (MQ=255)

                 tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGGCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                        >  1:1220335/1‑140 (MQ=255)

                  cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:2575398/140‑1 (MQ=255)

                  cGGTTTCACAGGCGGGTTTAAGCCATTTTTGACGCTGTCGTTTTTAGCTTTGCGTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:3265722/140‑1 (MQ=255)

                  agttttCACAGGCGGGTTTAATCCATTTTTGACGCTGTAGTTTTTAGCTTTGACTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  2:2682109/137‑1 (MQ=255)

                   ggTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCa                                                                                                                      <  2:805704/140‑1 (MQ=255)

                    gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATAAATTCACCAAATTTCAATGCAATAAAAAAATATTCAc                                                                                                                     >  2:5799930/1‑140 (MQ=255)

                       tCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTcc                                                                                                                  <  2:2239152/140‑1 (MQ=255)

                               gggTTTAATCCATTTTTGACGCTGTCGTTTTTTTCTTTTCCTGTCATTACTGACATAAAAATTAAGAAAACAGGATTATTTTATTTAAAATCATTTGAGCAAATTTCATTGAAATAAAAAAATATTCACTCCAAaatgat                                                                                                          >  1:3335410/1‑139 (MQ=255)

                                       tCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACAACAAAATTACGACAACAGGATTATTTGATGTAAAATCATTTAAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCtt                                                                                                  >  2:1519862/1‑140 (MQ=255)

                                           tttttGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCtttttt                                                                                              >  2:5409444/1‑140 (MQ=255)

                                           tttttGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAAATCAAAATGACGACAACAGGATTATTTTATGTAAAATAATTTCAGCATATTGCATTGCAATAAAAAAAGATTCACTCCAAATTGATTGCATCtttttt                                                                                              >  2:2061555/1‑140 (MQ=255)

                                           tttttGACGCTGTCGTTTTTAGCTTTGCCTGTAATTCATGACATCAAAATTAAGACAACAGGAGTATTTGATGTAAAATCAATTCCGCATAATGCATGTCAATAAAAAAAGATGCAAACCAAAGTGAGTGCCTCtttttt                                                                                              >  2:1035288/1‑140 (MQ=255)

                                            ttttGACGCTGTCGTGTTTTTCTGTGCCTTTCATTCCTGACATCAAAATGTCGACAACAGGATTTTTTTTTGTAAAATAATTTTATCAAATTTCATTGCAATAAAAAAAATTTGAATCCAAAGTGGGTGGATGttttttt                                                                                             >  1:2190585/1‑140 (MQ=255)

                                              ttGACGCTGTCGTTTTTAGCTTTGCCTGACATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTa                                                                                           <  1:4376744/140‑1 (MQ=255)

                                              ttGACGCTGTCGTTTTTAGCTGTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTa                                                                                           <  2:1185614/140‑1 (MQ=255)

                                                gACGCTGTCGTTTTTCGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTaaa                                                                                         <  2:4180970/140‑1 (MQ=255)

                                                     tGTCGTTTTTAGCTTTTCCTTTCATTCCTGACATCAAAATTACGACAAAAGGATTATTTTTTGTTAAATCATTTCAGCATATTTAATTGCAAAAAAAAAATATTCACTCCAAAGTTCTTGCATCTTTTTTTTAAAAttac                                                                                    >  1:3891898/1‑138 (MQ=255)

                                                           ttttAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCatgatg                                                                              <  1:4598647/140‑1 (MQ=255)

                                                                gCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAATTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCgg                                                                         <  1:2693455/140‑1 (MQ=255)

                                                                 cTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGa                                                                        >  2:3830216/1‑140 (MQ=255)

                                                                 cTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTAAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGa                                                                        <  1:602093/140‑1 (MQ=255)

                                                                   ttGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATATGATGTAAAATCATTTCAGCATATTGCATTGCACTAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCTGAAg                                                                      >  1:1816213/1‑140 (MQ=255)

                                                                    tGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAg                                                                                                                           >  1:3791234/1‑86 (MQ=255)

                                                                    tGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGTTGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAgg                                                                     >  2:1406570/1‑140 (MQ=255)

                                                                           cATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCg                                                              >  1:4997372/1‑140 (MQ=255)

                                                                           cATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCg                                                              >  1:1432934/1‑140 (MQ=255)

                                                                             ttCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGa                                                            <  1:226815/140‑1 (MQ=255)

                                                                             ttCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGa                                                            <  2:3725410/140‑1 (MQ=255)

                                                                                cTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACgg                                                         >  2:2624130/1‑140 (MQ=255)

                                                                                      tCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGAGCATCGGACGGcgtttc                                                   >  2:2919703/1‑140 (MQ=255)

                                                                                       cAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAGGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcg                                                  <  2:451314/140‑1 (MQ=255)

                                                                                        aaaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCCTTGCAATAAAAAAAGAAGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGTcgtttcgt                                                 >  2:3235190/1‑140 (MQ=255)

                                                                                        aaaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGCTCATCGGACGGcgtttcgt                                                 >  2:5359497/1‑140 (MQ=255)

                                                                                         aaaTGACGACAACAGGCTTATTTGAGGTAAAATCATTTCAGCATATTGCAGAGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCAAGATCATCGGAAGGCTCATCGGACGGcggttcgtt                                                >  2:3594405/1‑140 (MQ=255)

                                                                                         aaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgtt                                                >  2:3819728/1‑140 (MQ=255)

                                                                                         aaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgtt                                                >  2:1056705/1‑140 (MQ=255)

                                                                                         aaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgtt                                                >  2:1900885/1‑140 (MQ=255)

                                                                                         aaaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgtt                                                >  2:1543852/1‑140 (MQ=255)

                                                                                          aaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgttt                                               >  2:983086/1‑140 (MQ=255)

                                                                                          aaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgttt                                               >  2:138002/1‑140 (MQ=255)

                                                                                          aaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgttt                                               >  2:1072958/1‑140 (MQ=255)

                                                                                          aaTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgttt                                               >  1:834822/1‑140 (MQ=255)

                                                                                            tGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGttttt                                             <  1:4243843/140‑1 (MQ=255)

                                                                                            tGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGttttt                                             <  1:783538/140‑1 (MQ=255)

                                                                                             gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:1212180/140‑1 (MQ=255)

                                                                                             gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:4611256/140‑1 (MQ=255)

                                                                                             gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  1:3575042/140‑1 (MQ=255)

                                                                                             gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:5377748/140‑1 (MQ=255)

                                                                                             gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  2:249406/140‑1 (MQ=255)

                                                                                             gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            >  2:1749908/1‑140 (MQ=255)

                                                                                               cgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCa                                          <  2:1624129/140‑1 (MQ=255)

                                                                                               cgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGAATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCa                                          >  1:34089/1‑140 (MQ=255)

                                                                                                  caacaTGTTTATTTGATGTAAAATCATTTCAGCATTTTGCATTGCAATAAAATAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCatat                                       <  2:3790915/140‑1 (MQ=255)

                                                                                                       gtattattTGATGTAACATCATTTCAGCATATTGCATTGAAATAAAAAAAGTTTCACTCCAAAGTGAGTGCATGTTTTTTTTAAACTTCCATGATTATCGGAAGGATCATCGGACGGGGTTTCGTTTTTTCATATACGaa                                  <  2:5503157/138‑1 (MQ=255)

                                                                                                       ggATTATTTTATGTAAAATCATTTCAGCATATTTCATTTCAATAAAAAAATATGCACTCCAAAGTGAGTGAATCTTTTTTTTAAACTTCCATGATGATCGGAAGTATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:3306616/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTTCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACGTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:505007/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:803882/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:3918461/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:3966754/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:3124731/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:2508226/140‑1 (MQ=255)

                                                                                                       ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:5784476/140‑1 (MQ=255)

                                                                                                       ggATTATTGGATCTCACATCCTTTCAGCCTATTGCTTTGAAATAAAAAAAGAGTCACTCCAAAGTGAGTGCATCTTTTTTTTCAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:862010/140‑1 (MQ=255)

                                                                                                       ggATTATTAGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:3366257/140‑1 (MQ=255)

                                                                                                             tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTGTCGTTTTTTCATATACGAACGgcgc                            >  1:1774151/1‑140 (MQ=255)

                                                                                                             tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATATTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc                            >  2:1982281/1‑140 (MQ=255)

                                                                                                              ttGATTTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCAAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCa                           <  2:675029/140‑1 (MQ=255)

                                                                                                              ttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCa                           <  1:4650097/140‑1 (MQ=255)

                                                                                                              ttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCa                           <  1:5409444/140‑1 (MQ=255)

                                                                                                              ttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACGTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCa                           <  1:4813630/140‑1 (MQ=255)

                                                                                                              ttGATGTAAAAACATTTCAGCATATTGCATTGCAATAAAACAAGATGCACTCCACAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCa                           <  1:4111032/140‑1 (MQ=255)

                                                                                                                 aTGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGACCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGc                        <  2:3335410/140‑1 (MQ=255)

                                                                                                                  tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       <  2:4893683/140‑1 (MQ=255)

                                                                                                                  tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:1578541/1‑140 (MQ=255)

                                                                                                                  tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:1234794/1‑140 (MQ=255)

                                                                                                                    tAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCgtg                     <  1:1072958/140‑1 (MQ=255)

                                                                                                                     aaaaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCgtgt                    <  1:467969/140‑1 (MQ=255)

                                                                                                                      aaaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTc                   <  1:3366388/140‑1 (MQ=255)

                                                                                                                       aaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGAGGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCt                  >  2:403130/1‑140 (MQ=255)

                                                                                                                       aaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:3529115/124‑1 (MQ=255)

                                                                                                                       aaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:1050707/124‑1 (MQ=255)

                                                                                                                       aaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  >  2:693681/1‑124 (MQ=255)

                                                                                                                       aaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCt                  >  2:2447543/1‑140 (MQ=255)

                                                                                                                       aaTCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCt                  >  2:2183250/1‑140 (MQ=255)

                                                                                                                                  cATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCAtt       >  1:2095169/1‑140 (MQ=255)

                                                                                                                                     attgcattgcAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAg                                    <  2:799657/108‑1 (MQ=255)

                                                                                                                                     attgcattgcAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCAttttt    <  1:1015026/140‑1 (MQ=255)

                                                                                                                                       tgcattgcaATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGACGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCTAGCGTGTCTGTAATCTCATTTTTGa  >  1:4158431/1‑140 (MQ=255)

TGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGA  >  NZ_CP034943/1488046‑1488320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`