Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,730,084 G→C 17.7% D169H (GAT→CAT)  rodZ → cell shape determination protein RodZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0840GC17.7% 237.1 / 7.3 121D169H (GAT→CAT) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (58/30);  new base C (2/20);  total (60/61)
Fisher's exact test for biased strand distribution p-value = 1.33e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATT  >  NZ_CP034943/1729949‑1730218
                                                                                                                                       |                                                                                                                                      
aaTGCAGCAGCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACTCTAAAATATAATCAAAGCAAAAGCTCTGAAAAAGAGGCAGAAACTAAAAAAGAAACGAAAGAAAAAGAAGAGaa                                                                                                                                    >  1:1785994/1‑140 (MQ=255)
    ccGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTCAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaaa                                                                                                                                >  2:3393519/1‑140 (MQ=255)
      gCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGACACGAAAGAACAAGAAGATAAAAaaga                                                                                                                              >  2:1601108/1‑140 (MQ=255)
      gCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaaga                                                                                                                              >  2:3989886/1‑140 (MQ=255)
      gCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaaga                                                                                                                              >  2:2867967/1‑140 (MQ=255)
       cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                             >  2:1059813/1‑140 (MQ=255)
               aaaTCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGa                                                                                                                     >  2:448113/1‑140 (MQ=255)
                    aaTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTg                                                                                                                >  1:4439102/1‑140 (MQ=255)
                    aaTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTg                                                                                                                >  1:2738529/1‑140 (MQ=255)
                                     caaaGAACGAAATCCCAAAAGATAAAACGCAAAAACAAAACAAAACCAACCGCTCTGAAAAAGTGCCAGACACAAAAAACCAAACCAAACAAAAACAACAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                               <  1:237196/138‑1 (MQ=255)
                                     acAATTACTAAATCCAAAAATCTTCAACGCTAAAAGACAATAAAAGCAACAGCTATTAAAAAGAGGCATACACTAAAAAATAAAACAAAGAAAAACACTATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTaaa                                                                                               <  2:5527826/139‑1 (MQ=255)
                                        aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                            >  2:5493047/1‑140 (MQ=255)
                                        aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                            >  1:4723827/1‑140 (MQ=255)
                                        aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTCATAAACTGGAGATTAAAGCa                                                                                            >  2:4253835/1‑140 (MQ=255)
                                          tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                          >  2:736888/1‑140 (MQ=255)
                                            ctaaaTCCCAAAAGATTCAACGCTAAAAGAGAATAAAAGAAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACTAAACAAAAAGAAGATAAAAAACACGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                        <  2:2738529/138‑1 (MQ=255)
                                            ctaaaTCACAAAAGATTAAACGCTAAAAGATAATAAAAGCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACCAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                        <  2:4439102/138‑1 (MQ=255)
                                            ctaaaTCAAAAAATATACAACGATAAAATACAAAAAAAGAAACATAGCTTAAAAATAGGCATAAACTAAAACAGAAACTAAACACAAACAACATAAAAACCACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                        <  2:2955966/138‑1 (MQ=255)
                                            cGAAATCCAAAAATATCCAAGGCTAAAAGACAATAAAAGAACCAGATCTGAAAATGAGGAATACACTAAAAAATAAACGAAAGAAAAACACGTTAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                        <  2:377077/140‑1 (MQ=255)
                                              aaaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACATCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGAAAGTGATAAACTGGAGATTAAAGCAACTGGc                                                                                      <  2:2862153/140‑1 (MQ=255)
                                              aaaGCCCAAACTATTCAACGCTAAAACAGAAACAACGAAACAGCTGTCAAAAAGAGGCACAAAATAAAAAACAAACCACACAAAAACAACATAAACACCAACAACGCGACAGGGATAAACTGGAGATTAAAGCAACTGGc                                                                                      <  1:1345363/140‑1 (MQ=255)
                                                 tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAATAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                   >  1:197759/1‑140 (MQ=255)
                                                 tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                   >  1:1605662/1‑140 (MQ=255)
                                                    cAAAAGATCCAACGCTAAAAGAGAATAAAAGCAACAGATCTGAAAAAGAGGAACACACTAAAAAAGAAACCAAAGAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                <  2:1830149/140‑1 (MQ=255)
                                                    cAAAAGATACAACGCTAAAACAGAATCAAACCAACAGCTCTCAAAAACAGGCAGACACTAAAAAATAAACCAAACAAAAACAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                <  2:1753993/140‑1 (MQ=255)
                                                           ttCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAt                                                                         >  2:4077343/1‑140 (MQ=255)
                                                           ttCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAt                                                                         >  2:865700/1‑140 (MQ=255)
                                                           ttCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAt                                                                         >  2:1160147/1‑140 (MQ=255)
                                                           ttCAACGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGAGATAAACTGGAGATTAAAGCAAATCGCACTGAAGCGTCAt                                                                         >  2:1007574/1‑140 (MQ=255)
                                                               cctatAAAACAGAAACAAAGCAACAGCTCTAAAAAAGAGGAATACACTAAAAAAGAAACCAAACAAAACTAACATAAAAACTACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:3109186/136‑1 (MQ=255)
                                                               acgacaaaaCAGAAACAACGCAACAGCTCTCAAAAAGAGGCAGACACTAAAAAACACACCAACCAAACACAAGATAAAAACCACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:5384922/135‑1 (MQ=255)
                                                               accatAAAACAAAATCAAAGAAACAGCTATCAAAAAGAGCCAGACAATAAAAAACAACCCAAACAAAAACAATATAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:1682600/136‑1 (MQ=255)
                                                               aCGCTAACAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAAGAAGATAAAAACGAAGCAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  2:1433266/140‑1 (MQ=255)
                                                               aCGCTAAAAGATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  2:3700290/140‑1 (MQ=255)
                                                               aCGCTAAAAGAGAATAAACGCAAACGCTCTCAAAAATCGGCAGACACTAAAAAAGAAACGAAACAAAAAGACCATAAAAACCAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:2570826/140‑1 (MQ=255)
                                                               aCGCAAAAAGACAATCAACCAAACAGCACGTAAAAACAGGCACACACTAAAAAACAACCCAACGAAAAACAACAGAAAACAGAACCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:1059813/140‑1 (MQ=255)
                                                               aCGCAAAAACACAAACAAACCAACAGCTCTTAAAAACAGCAATAACCTAAAAAACAAACCAAAAAAAACCAACAAAAAAAACAACAACGCGCCAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:5383959/140‑1 (MQ=255)
                                                               aCGATAAAAGAGAATCAAAGCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACTAAAGAAAAAGAAGATAAAAAAGCCGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  2:5775999/140‑1 (MQ=255)
                                                               aCGATAAAAGAGAATAAAAGAAACAGCTCTTAAAAAGAGGCACACACTAAAAAACAAACCAAACAAAACGAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                     <  1:4217651/140‑1 (MQ=255)
                                                                 gatAAAATACAATCAAACAAACAGCTATTAAAAAGAGGAAGACACTAAAAAATAAACGAAACAAAAAGAACATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGa                                                                   <  2:603917/138‑1 (MQ=255)
                                                                  atAAAAGATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAAGAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGAc                                                                  <  2:3526311/139‑1 (MQ=255)
                                                                   tAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACt                                                                 >  2:2233411/1‑140 (MQ=255)
                                                                    aaaaGATAATAAAACAAACATCTCTTAAAAATTGCAACAAAATAAAAAATAAACCAAAAAAAAATAATAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACtt                                                                <  1:5356574/140‑1 (MQ=255)
                                                                         acaaTCAAACCAACACCTCTCAAAAAGAGCCCCACACTAAAAAAGAAACCACCCAACACCACCAGAAAAACCAAGAACGCGACAGAGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                           <  1:3742299/138‑1 (MQ=255)
                                                                           aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                         >  1:4916343/1‑140 (MQ=255)
                                                                             tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                       >  1:5280044/1‑140 (MQ=255)
                                                                             tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                       >  1:4493626/1‑140 (MQ=255)
                                                                             tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                       >  1:4246255/1‑140 (MQ=255)
                                                                             tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                       >  1:1260226/1‑140 (MQ=255)
                                                                             tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGAGAAAAAAGAAGAAAGCGACAGTGATCAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                       >  1:4161839/1‑140 (MQ=255)
                                                                                 aGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCgg                                                   >  2:888846/1‑140 (MQ=255)
                                                                                     aCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCt                                               >  1:221143/1‑140 (MQ=255)
                                                                                        gCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgag                                            >  2:2500207/1‑140 (MQ=255)
                                                                                        gCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgag                                            >  2:4633034/1‑140 (MQ=255)
                                                                                        gCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTTGCACTGAATGGTCATTAACGACTTAAGACTTGTCAGGCGCTgat                                            >  2:4355100/1‑139 (MQ=255)
                                                                                         ctctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                                           >  1:620942/1‑140 (MQ=255)
                                                                                           ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGaaa                                         >  2:2539384/1‑140 (MQ=255)
                                                                                           ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGaaa                                         >  1:539177/1‑140 (MQ=255)
                                                                                           ctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGaaa                                         >  2:1652371/1‑140 (MQ=255)
                                                                                              aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                      >  1:3240323/1‑140 (MQ=255)
                                                                                              aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                      >  1:326033/1‑140 (MQ=255)
                                                                                                      gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGa                              >  1:640795/1‑140 (MQ=255)
                                                                                                      gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGa                              >  1:2714765/1‑140 (MQ=255)
                                                                                                      gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGa                              >  1:423963/1‑140 (MQ=255)
                                                                                                       cAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGaa                             <  2:5606988/140‑1 (MQ=255)
                                                                                                       cAGACAATAAAAAACAAACGAAACAAAAACAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGaa                             <  2:4759601/140‑1 (MQ=255)
                                                                                                       aataaacTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGaa                             <  2:3814259/135‑1 (MQ=255)
                                                                                                           ccatAAAAACCAACCCAAAAAAAAAGACCATAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:771197/137‑1 (MQ=255)
                                                                                                           cacaAAAAAAGAAACCAAATAAAAAGAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:1371903/140‑1 (MQ=255)
                                                                                                           cacTAAAAAACAAACCAAAGAACAACAAGACAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:2600186/140‑1 (MQ=255)
                                                                                                           cacTAAAAAAAAAACCAAACAAAAATAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:1968814/140‑1 (MQ=255)
                                                                                                           aacTAAAAAATAAACGAAAGAAAACGAACATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCAATGACGGGGCATTAACGACTTATGAAGTGTCCGGCGCTGAGACATTCGAGCTCGAACTGa                         <  2:4917975/139‑1 (MQ=255)
                                                                                                           aacTAAAAAAGAAACGAAATAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  2:1999683/139‑1 (MQ=255)
                                                                                                           aaatAAAAAACAACCGAAACAAAAACAATATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:1580966/137‑1 (MQ=255)
                                                                                                           aaatAAAAAACAAACTAAAGAAAAAGAAGATAAAAAAGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  2:2158731/137‑1 (MQ=255)
                                                                                                           aaatAAAAAACAAACTAAAGAAAAACAAAATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:2860658/137‑1 (MQ=255)
                                                                                                           aaaaaaaaaaCAAACTAAACAAAAACAATATAAAAACCAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                         <  1:2304656/136‑1 (MQ=255)
                                                                                                             cTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAg                       <  2:4208151/140‑1 (MQ=255)
                                                                                                             atAAAAAACAAACGAAACAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAg                       <  2:5285307/139‑1 (MQ=255)
                                                                                                                aaaaaTAAAATAAACAAAACGAACACAAAAAACAACCACGCGACAGTGATAAACGGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGATGAGAAATTCGAGCTCGAACTGAAGGCa                    <  1:5685975/140‑1 (MQ=255)
                                                                                                                aaaaaCAAAGGAAACAAAAAGAAGATACAAACCAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                    <  1:3888699/140‑1 (MQ=255)
                                                                                                                aaaaaCAAACCAAACAAAAACACCCTAAAAAAGAACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                    <  1:4731560/140‑1 (MQ=255)
                                                                                                                     taaaCCAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa               <  1:2775727/139‑1 (MQ=255)
                                                                                                                     caaaCGAAAGAAAAACAATATAAAAAATAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa               <  2:2046709/139‑1 (MQ=255)
                                                                                                                     caaaCCAAACAAAAATAACATAAAAAACACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa               <  1:2220724/139‑1 (MQ=255)
                                                                                                                     caaaCCAAACAAAAAGAAGATAAAAAACACCACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa               <  1:930511/139‑1 (MQ=255)
                                                                                                                     caaaCCAAACAAAAAGAACATAAAAAATAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa               <  1:3989886/139‑1 (MQ=255)
                                                                                                                      aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc              >  1:187873/1‑140 (MQ=255)
                                                                                                                       aaCTAAATAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa             <  2:3644551/140‑1 (MQ=255)
                                                                                                                       aaCTAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa             <  2:1190653/140‑1 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  1:1279463/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  1:5578041/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  1:2510281/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  2:1781382/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  1:3302421/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  2:3344325/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  1:3318098/1‑140 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAACATAAAAAAGAATAAAGCGACATTGATAAACTGGAGATTAAAGCAACTGGCACTCAAGTGTCATTAACGACTTATCAAGTGTCAGGCGCTGAGAAATTCGAGATCGAACTGAAAGCATCAGACac            >  2:3002141/1‑139 (MQ=255)
                                                                                                                        aCGAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg            >  2:836828/1‑140 (MQ=255)
                                                                                                                                  cagaagaTAAAAACGACGAACGCGACAGTGATAAACTGGAGAGTAAAGCAACGGGCACTGAAGGGTCATTAACGACTTATGAAGTGGCCGGCGCTGAGAACTTCGAGCTCGAACTGAAGGCATCAGACAGTTATTGGAtt  <  2:2502339/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATT  >  NZ_CP034943/1729949‑1730218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: