Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,753,598 G→T 9.2% intergenic (+289/‑216) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,753,5980GT 9.2% 827.0 / 10.7 366intergenic (+289/‑216)fabD/EO946_RS09090ACP S‑malonyltransferase/acyltransferase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (135/182);  new base T (46/2);  total (182/184)
Fisher's exact test for biased strand distribution p-value = 1.48e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 367 total aligned reads displayed.

AAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATT  >  NZ_CP034943/1753459‑1753735
                                                                                                                                           |                                                                                                                                         
  aaCATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGtt                                                                                                                                         >  1:4192333/1‑140 (MQ=255)
      tGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTatat                                                                                                                                     <  2:900322/140‑1 (MQ=255)
      tGTCCACAAGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTatat                                                                                                                                     <  2:810454/140‑1 (MQ=255)
        tCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCa                                                                                                                                   <  2:202270/140‑1 (MQ=255)
        tCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCATTTTTGAAACAGTCATTAAGTTatataa                                                                                                                                   >  2:5203532/1‑138 (MQ=255)
               gATATTTGAACTATGTCAGTCCTCCTGCAGATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTga                                                                                                                            <  2:3599955/140‑1 (MQ=255)
               gATATTTGAACTATGTCAGTCAGCCGGCATATGATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTga                                                                                                                            <  2:5342440/140‑1 (MQ=255)
               gATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTTTCCTGTCATTATGTTATATCAATTTTga                                                                                                                            >  2:5144163/1‑140 (MQ=255)
                 tatTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTgaga                                                                                                                          >  2:5238697/1‑140 (MQ=255)
                    ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                                       <  2:3961071/140‑1 (MQ=255)
                    ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                                       <  1:4809228/140‑1 (MQ=255)
                    ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                                       <  2:217331/140‑1 (MQ=255)
                    ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                                       <  2:3650308/140‑1 (MQ=255)
                         cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATACTGTCATTATTTTATTTAAATTTTGATATATCaaaa                                                                                                                  >  2:1206381/1‑138 (MQ=255)
                         cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:4713711/140‑1 (MQ=255)
                         cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:1613698/140‑1 (MQ=255)
                         cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:3592059/140‑1 (MQ=255)
                         cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:4564623/140‑1 (MQ=255)
                         cTATGTCAGTCAGCCGGCATATCATGATAGACCAACGCTCTCAACTCAAGATGGCCTGCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTGGTGATCCTGTCATTATATTCTAACACATTTGAGATATCacac                                                                                                                  >  2:3364834/1‑137 (MQ=255)
                          tATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCAGTCATTATGTTATATCAATTTTGAGATATCAACAt                                                                                                                 <  1:2828194/140‑1 (MQ=255)
                           aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTCGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTGTATCAAATTTGAGATATCAACATa                                                                                                                >  2:1227781/1‑140 (MQ=255)
                           aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATCCTGTCATTATTGTATATCAATTTTGAGATATCAAAATa                                                                                                                >  2:2615028/1‑140 (MQ=255)
                           aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGTCATTATGTTATATAAATTTTGAGATATAAACaaa                                                                                                                >  2:2234027/1‑138 (MQ=255)
                           aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTTATCCTGTCATTATGTTATATCAATTTTGATATATCAAAATa                                                                                                                >  2:3005026/1‑140 (MQ=255)
                            tGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATac                                                                                                               <  1:1325326/140‑1 (MQ=255)
                               cagtcagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACAcc                                                                                                            <  2:5495569/140‑1 (MQ=255)
                                    agCCTGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:1346867/140‑1 (MQ=255)
                                    agCCGTCATATCATGATAGACCATCGCTCTCAACTCAAGATTGGCTTATATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATTTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:4495158/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGTTGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:4833236/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:1957276/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:4712381/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:4342962/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:631127/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:104017/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:5403504/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:2615028/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:4480595/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:637037/140‑1 (MQ=255)
                                    agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:4426905/140‑1 (MQ=255)
                                      ccggactatCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCAAAATCTTAGCGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  1:861245/134‑1 (MQ=255)
                                      ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACAt                                                                                                                 <  2:4644275/128‑1 (MQ=255)
                                      ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  1:3414363/140‑1 (MQ=255)
                                      ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  2:4381986/140‑1 (MQ=255)
                                      ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  2:4961162/140‑1 (MQ=255)
                                      ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTGATCCTGTGATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     >  1:3886527/1‑140 (MQ=255)
                                         gCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACtt                                                                                                  <  1:5031762/140‑1 (MQ=255)
                                         gCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATACCAACATACACCTAGACCACtt                                                                                                  <  2:2443260/140‑1 (MQ=255)
                                                aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                           <  2:172523/140‑1 (MQ=255)
                                                aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                           <  2:4192333/140‑1 (MQ=255)
                                                aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                           <  2:4261016/140‑1 (MQ=255)
                                                   aTAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCTGTAATTATGTTATATAAATTTTGAGATATCAACATACAACTATAACACTTCATAATagag                                                                                        >  2:1551512/1‑140 (MQ=255)
                                                   aTAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATTTTATATCAATTTTTATATATAAAAATACACCTAGACCACTTCATAATagag                                                                                        >  2:3816010/1‑140 (MQ=255)
                                                    tAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGTTCCTGTAATTATGTTATATCAATTTTGAGATATGAACATACACCTAGACCACTTCATAATAGAGc                                                                                       >  1:5467427/1‑140 (MQ=255)
                                                    tAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTTCTCCTTTCAATATGTTATATGAATTTTGAGGTTTCAACCAAAACCTAGACCACGTCATAATTGAGc                                                                                       >  1:5112426/1‑140 (MQ=255)
                                                       aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:4570284/121‑1 (MQ=255)
                                                       aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTGTTCCGGTCCTTATGTTATATCAATTTTGAGATATCAACATAAACCTAGAACACTTCATAATAGAGCCGc                                                                                    >  2:730355/1‑140 (MQ=255)
                                                         cATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTCTCCGGTCATTATGTTATATAAATTTTTAGATATCAAAATAAACCTAGAACACTTCATAATAGAGCCGCAt                                                                                  >  2:3491555/1‑140 (MQ=255)
                                                         cATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACtt                                                                                                  >  2:4302165/1‑124 (MQ=255)
                                                         cATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTTTTGATCCTGTGATTATGTTATATCAATTTTGAGTTATAAACATACACCTAGACCACTTCATAAAAGAGCCGAAt                                                                                  >  2:2362152/1‑140 (MQ=255)
                                                         cATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGGCGTTGTTGATCCTGGCAATATGTTTTATCAAATTTGAGCTATCAACATACACCTAGACCACGTCCTAAGAGTGCCGcct                                                                                  >  2:5427377/1‑138 (MQ=255)
                                                           tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATAAACCGAGACCACTTCATAATAGAGCCGCCTcc                                                                                >  1:3545890/1‑140 (MQ=255)
                                                           tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATcc                                                                                >  2:5614645/1‑140 (MQ=255)
                                                           tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATcc                                                                                >  2:4086326/1‑140 (MQ=255)
                                                           tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTGATGCTGTCATTATGTTATATCAATTTTTATATTTCAACATACACCTAGACCACTTAATAATATAGCCGcctac                                                                                >  1:3288200/1‑136 (MQ=255)
                                                           tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGGTGTTTGTTGTTCCTGGCATTATGTTATATCAATTTTGTGATATAAACAAAAACCTAGACCACGTCATAAAATATCCGCATcc                                                                                >  1:2890244/1‑140 (MQ=255)
                                                            cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGTTCCTGTGATTATGTTATATGAATTTTGTGATATAAACATACAACTAGACCCCTTAATAATAGAGcccccacca                                                                               >  1:1875382/1‑133 (MQ=255)
                                                            cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCa                                                                               <  2:5796153/140‑1 (MQ=255)
                                                                   aactcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCAcc                                                                        <  2:5694995/140‑1 (MQ=255)
                                                                   aactcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCAcc                                                                        <  1:2234027/140‑1 (MQ=255)
                                                                   aactcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGGTATATCAATTTTGTGATATCAACCTACACCTAGACCACTTCATAATATAGCCGCATCCAGAGCaac                                                                        >  2:415501/1‑138 (MQ=255)
                                                                   aactcaaGAGGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCAcc                                                                        <  1:992413/140‑1 (MQ=255)
                                                                       caaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCAATATGTTATATCAATTTTTAGATATCAACAAAAAACTAGACCACTTCATAATATAGCCGCATACAGAGCACCaaaa                                                                    >  2:1152493/1‑140 (MQ=255)
                                                                        aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAg                                                                   >  2:227446/1‑140 (MQ=255)
                                                                        aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAg                                                                   >  2:3163383/1‑140 (MQ=255)
                                                                          gATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCa                                                                 <  1:1745945/140‑1 (MQ=255)
                                                                             ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTTTTCCTGGCATTATGTTATATCAATTTTGAGATATAAACATACACCGAGACCACGTCATAATAGAGCCGCATCCCGAGCCCCAAAAGccaac                                                              >  1:4149586/1‑136 (MQ=255)
                                                                             ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTTATCCTGTCATTATTTTATATGAATTTTGTGATATAAAAATAAACCAAGAACAAGTCATAATAGATCCGCATCCACAGCAACAAAAACaaaa                                                              >  1:558124/1‑138 (MQ=255)
                                                                             ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                                              <  2:252211/140‑1 (MQ=255)
                                                                             ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTGTTGATCCGGTCATTATTGTATATAAATTTTGAGAAATCAACATAAACCTAGACCCCATCATAATAGAGCCGCATCCAGAGCACCAAAAGCaaac                                                              >  1:1376956/1‑138 (MQ=255)
                                                                             ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGGTCTTTGTTTATCCTGTGATTATTTTATATCAATTTTGTGATATCAACAAACAACGAGACCCCTTCAGAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                                              >  1:4638921/1‑140 (MQ=255)
                                                                               ccTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATGCTGTCATTATGTTATATAAATTTTTAGATATCAACATACACCTAGACCACTTCATAATATAGCCGCATCCAGAGCACCAAAAGCAAaccg                                                            >  1:1099894/1‑139 (MQ=255)
                                                                               ccTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACAACTAGACCACTTCATAATATAGCCGCATCCAGAGCACCAAAAGCAAtcct                                                            >  2:2954799/1‑140 (MQ=255)
                                                                                   cTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTa                                                        <  1:5725759/140‑1 (MQ=255)
                                                                                   cTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTa                                                        <  1:735682/140‑1 (MQ=255)
                                                                                              cACCCAAATCTTAGAGTCGATGTTGTTTTTTTATCCTGTCATTATTTTATATAAATTTTTAGATATAAACATAAACCGAGACCACATGATAAAATAGCCGCATCCACAGCACCAAAAGCAAACCGCCTATTAGTTGAttg                                             >  1:367414/1‑139 (MQ=255)
                                                                                              cACCCAAATCTTAGAGTCGATGATCTTTGTTGATGCTGTAATTATGTTATATCAAATTTGTGATATCAACCTAACCCAAGACCACGGCCTAATAGAGCCGCCTCCAGAGCAGCAAAAGCAAGCCGCCGATTAGGTGGtta                                             >  2:5458751/1‑139 (MQ=255)
                                                                                              cACCCAAATCTTAGAGTCGAGGTTCTTTTTTGTTACGGTCATTATGTTATATCAAATTTTAGATATCCACATAAACCTAACACACTTGATAATAGAGCCCCATCCAGACCACCAAAAGCAAACCGCCTAGTAGTTGAttc                                             >  1:2049965/1‑140 (MQ=255)
                                                                                                  cAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGCGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATtcttct                                         <  1:3913056/140‑1 (MQ=255)
                                                                                                         tAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                                  <  1:5238697/140‑1 (MQ=255)
                                                                                                          agagTCGATGTTCTTTTTTGTTCCTGTCATTATTTTATATCAATTTTGAGATATCAACCTACACCAAGACCACTTCATAAAAGAGCCCCATCCAGATCACCAAAAACAAACCTCCTCTTAGTTGATTCTTCTTAtttttt                                 >  1:3970892/1‑140 (MQ=255)
                                                                                                                          gttgATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCa                 <  2:2674637/140‑1 (MQ=255)
                                                                                                                           ttgATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCaa                <  1:3098478/140‑1 (MQ=255)
                                                                                                                              aTCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                                  >  1:2428290/1‑119 (MQ=255)
                                                                                                                              aTCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTa             >  1:831642/1‑140 (MQ=255)
                                                                                                                              aTCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTa             >  2:4936016/1‑140 (MQ=255)
                                                                                                                                 cTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAat          >  2:1840978/1‑140 (MQ=255)
                                                                                                                                   gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat        >  1:2360025/1‑140 (MQ=255)
                                                                                                                                   gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat        <  2:2798235/140‑1 (MQ=255)
                                                                                                                                    tCATTATGTTATATCAATTTTGAGCTATCAACATACACCTAGACCACTTCATAATAGAGCCGCCTCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATGCTTCTTATTTTTTACAGTATGTCTTTTCAATTCAGTATc       >  2:3708073/1‑140 (MQ=255)
                                                                                                                                         aTGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACAtt  <  1:1228369/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATT  >  NZ_CP034943/1753459‑1753735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: