Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,586 G→T 7.4% intergenic (+264/‑40) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5860GT 7.4% 1314.1 / 20.8 517intergenic (+264/‑40)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (146/314);  new base T (55/2);  total (201/316)
Fisher's exact test for biased strand distribution p-value = 1.15e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 521 total aligned reads displayed.

TTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAC  >  NZ_CP034943/112448‑112725
                                                                                                                                           |                                                                                                                                            
   gAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTg                                                                                                                                          <  2:2094047/140‑1 (MQ=255)
   gAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTg                                                                                                                                          <  1:174160/140‑1 (MQ=255)
         aGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAgg                                                                                                                                   <  1:4067610/140‑1 (MQ=255)
         aGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAgg                                                                                                                                   <  2:1116486/140‑1 (MQ=255)
                      ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGCAATCAAATagag                                                                                                                      <  2:1037129/140‑1 (MQ=255)
                      ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                      <  1:3904912/140‑1 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAATAGGAAATCAAATAtaga                                                                                                                     >  1:3703434/1‑140 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATTCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATGAAATagaca                                                                                                                     >  2:4047712/1‑138 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATc                                                                                                                              <  2:5411888/131‑1 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATc                                                                                                                              <  2:2719960/131‑1 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAgaga                                                                                                                     >  1:3324071/1‑140 (MQ=255)
                        ccgccgccgTTACAGGCTTAAGTTGGGAGAGCATAAGTATGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTATCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCACATAGAGaa                                                                                                                    <  2:2630574/140‑1 (MQ=255)
                        ccgccgcagTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGaa                                                                                                                    <  2:2177148/140‑1 (MQ=255)
                         cgccgccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAg                                                                                                                   <  2:398317/140‑1 (MQ=255)
                                 ttACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATATAGAAGTGGGGGGa                                                                                                           >  2:126237/1‑140 (MQ=255)
                                    cAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                                                                        <  2:4148875/140‑1 (MQ=255)
                                    cAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                                                                        <  1:1246066/140‑1 (MQ=255)
                                     aGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCa                                                                                                       <  1:1878722/140‑1 (MQ=255)
                                      ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGAGGTTTAAGCGTCTCTGTCATTTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                                                                                      <  2:1771994/140‑1 (MQ=255)
                                             gTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGtttt                                                                                               <  1:4104326/140‑1 (MQ=255)
                                                  gagaGCAGAATTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                          <  1:3953119/140‑1 (MQ=255)
                                                  gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                          <  1:859274/140‑1 (MQ=255)
                                                  gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                          <  1:4541761/140‑1 (MQ=255)
                                                     agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATg                                                                                       <  2:642817/140‑1 (MQ=255)
                                                        aGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTc                                                                                    <  1:2047884/140‑1 (MQ=255)
                                                        aGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTc                                                                                    <  1:4418506/140‑1 (MQ=255)
                                                          aaGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTc                                                                                    >  2:5728330/1‑138 (MQ=255)
                                                            gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                <  2:3324071/140‑1 (MQ=255)
                                                              cttcTTTTCAAACAGAGTGGAACCGCGCGTTTTAAGCGTATCTGTAATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:2500938/137‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGTTTTAAGCGTCTCTGTCATGTGTTCATGCTGAGGCGCTTTTTTTATTGGGTGAAGCGGAAAGCAAATAGAGAAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:3650363/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:183275/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:1519462/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:2824374/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:1666306/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:4297032/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAACCATGTGTTTGTTAGAATGCTCAaagaa                                                                              <  1:4630307/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:5678413/140‑1 (MQ=255)
                                                               tGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGTAGAGGTAATCAAATATAGAAGTGGGGGGCAGCATGTGTTTTTTATAATTCTCAaataaa                                                                             >  1:4516518/1‑139 (MQ=255)
                                                               tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTTGGGGGACGCCGGTGTTTTTTTTAAATCTCAaagaag                                                                             >  1:2722984/1‑140 (MQ=255)
                                                               tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGGAGGGGGAAACCAATAGAGAAGGGGGGGGGAGCATGTGTTTTTTTGTATTCTCAaagaag                                                                             >  1:1927913/1‑140 (MQ=255)
                                                                gattttAAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGAGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:5440837/138‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:5098966/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:525860/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:708337/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTAATGCCGAGTCGCTTTTTTTAGTTGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  2:4839089/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTGTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  2:2309211/140‑1 (MQ=255)
                                                                 cTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGTAATCAAATCTAGAAGTGGGGGGAAGCATGTGTGTTTTAGAATGCTCAAACAAGAc                                                                           >  2:1423663/1‑140 (MQ=255)
                                                                 cTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGAc                                                                           <  1:4695676/140‑1 (MQ=255)
                                                                 cTTTTCAAACAGAGGGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAATGGGTGAAGAGGAAATAAAAAAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTGAAAGAAAAc                                                                           >  2:1997129/1‑140 (MQ=255)
                                                                      cAAACAGAGTGGAACCGCGCGGTGTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGATTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCCCAAAGAAGACATTGa                                                                      <  2:3712427/140‑1 (MQ=255)
                                                                        aaCAGCGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTGTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  1:3123116/140‑1 (MQ=255)
                                                                        aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAACTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:2958181/140‑1 (MQ=255)
                                                                        aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  1:564014/140‑1 (MQ=255)
                                                                        aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  1:5590698/140‑1 (MQ=255)
                                                                        aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:4660921/140‑1 (MQ=255)
                                                                        aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  1:5768382/140‑1 (MQ=255)
                                                                         aCAGTGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTATTGGGTGAAGAGGAAAACACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  1:1015190/140‑1 (MQ=255)
                                                                          cAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACa                                                                  <  2:480134/140‑1 (MQ=255)
                                                                           agagTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCCAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAATAAGAAATTTATACAg                                                                 >  2:4414831/1‑140 (MQ=255)
                                                                             agTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAgtg                                                               >  2:3578639/1‑140 (MQ=255)
                                                                              gTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTGGGTGTATAGGAAAAAAAAAATAGTAGTGGGGGGAAGCACTTGTTTTTTATTATTCTCAAAAAAAAAAATTATAAAgtgt                                                              >  1:2839895/1‑140 (MQ=255)
                                                                               tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                             <  1:2593778/140‑1 (MQ=255)
                                                                                   aCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAACTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAt                                                         <  2:84211/140‑1 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTCGCGGGCAACGAATATAGAGCTGGGGGGCAGCATGGGTTTTTTATTATGGTCAAAGCAGACAGTGTTACACTGTTGGagaaat                                                     >  1:3456339/1‑135 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGCACTCAAATAGAGAAGGGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATCAGGTGTTCGATCAAg                                                     >  2:3096455/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCATAGAAGAAATTGATAAAGTGTTCGATCaat                                                     >  2:5479274/1‑139 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            >  2:1908307/1‑117 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACACTGTTAGATCAAg                                                     >  2:3208530/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATATAGAACTGGGGGGAAGCAAGTGTTTTTTAGAATGGTCAAAGAAGACAATGATACACTGTTAGATCAAg                                                     >  2:778638/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGAAGAGGAAAAAAAATATAGAAGTGGGGGGGAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATAAAGTGTTGGATCAAg                                                     >  1:3048327/1‑140 (MQ=255)
                                                                                          ggTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATc                                                  <  2:1182584/140‑1 (MQ=255)
                                                                                               aaGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGGATAGGAAATCAAAAAGAGAAGGGGGGGGAACCATGTGTTTTTTATAATGCTCACAGAAGACATTGATACAGTGTTCGATCAATATGgagca                                             >  1:3819362/1‑140 (MQ=255)
                                                                                                  cGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAgcagca                                          <  1:4931568/140‑1 (MQ=255)
                                                                                                    tctcTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaag                                        <  1:772569/140‑1 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGATGGAAAGCAATAGAGGAGTGGGGGGGAGCCTGTGTTTTTTATAAAGGTCAAAAAAAACAATGATACAGTGTTCGATCAAGATACCGCCGCaaaga                                      >  2:4295712/1‑137 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGGATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                      >  2:2769936/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATGAAATAGGGAAGTGGGGGGACGCCTGTGTTTTTTAGAATGGTCCAAGAAGACATTGATCCAGTGTTCGATCATGATCCAGCAGCgagaa                                      >  2:5174439/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGATAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTAGATCAAGATGCAGCAGCaagaa                                      >  2:3850402/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAGTGTTCGATAAAGATACAGCACcaacaa                                      >  2:5081363/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGGAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCCTGTGTTTTTTATAATTCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                      >  2:347593/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGTAGAGGGAAACAAATAGAGAAGTGGGGGGGAGCATGTGTTTTTTTGAATTCTCAAAGAAGACAATGATACACTGTTCGATAAAGATACAGCAGCaagaa                                      >  1:1079286/1‑140 (MQ=255)
                                                                                                      tctGGCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGCACACGAAATCAAATAGCGAAGTGGGGGGAAGCATGGGTTTTTTAGAATGCTCAAATAAGACCTTGATACAGTGTTCGATCCAGATCCAGCCGCaagaa                                      >  2:163565/1‑140 (MQ=255)
                                                                                                       ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                     <  1:1401771/140‑1 (MQ=255)
                                                                                                       ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                     <  1:2162861/140‑1 (MQ=255)
                                                                                                       ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAAGAGAGAAGTGGGGGGAAGCATGTGTTGTTTACAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                     <  1:338933/140‑1 (MQ=255)
                                                                                                       ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                     <  2:3912127/140‑1 (MQ=255)
                                                                                                             tGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTTTTACAGTGTTCGATCAAGATACAGCAGCAACAAGTTAttt                               >  2:1537240/1‑140 (MQ=255)
                                                                                                               ttttCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAAAAAAAAATAGTAGTGGGGGGGAGCATGTGTTTTTTTGAATTCTCAAAGAAAAAAATTATAAAGTGTTAGATAAAGATACACCCGCAAGAAGTTAttttt                             >  1:2988601/1‑139 (MQ=255)
                                                                                                               ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            >  2:341610/1‑93 (MQ=255)
                                                                                                                 ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  1:63720/140‑1 (MQ=255)
                                                                                                                    aTGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGCAATCAAATAGAGTAGTGGGGGGAAGGCTGTGTTTTTTAGAATGCTGAAAGAAGACATTGATAAAGTGTTAGATCAACATCCAGCAGCACGAAGTTATtgtgtcgtt                        >  2:3494558/1‑135 (MQ=255)
                                                                                                                    aTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGCAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAGTGTTCGATCAAGATCCAGCAGCCAGAAGGTATTTTGAAGTg                        >  2:4959547/1‑140 (MQ=255)
                                                                                                                          agGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTa                  >  2:5543199/1‑140 (MQ=255)
                                                                                                                            gcgcTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAAc                <  1:2415832/140‑1 (MQ=255)
                                                                                                                             cgcTTTTTTTCTTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACt               <  1:420787/140‑1 (MQ=255)
                                                                                                                                  tattttATTGGCTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTAtt           <  2:905039/138‑1 (MQ=255)
                                                                                                                                   ttattATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc          <  1:5043758/137‑1 (MQ=255)
                                                                                                                                        aTTGGGTGCAGAGGAAATCACCTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCGCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTCTTTTGAAGTGATTTTAACTTATTCCGGtt     <  1:4747076/140‑1 (MQ=255)
                                                                                                                                          tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa   <  2:4516592/140‑1 (MQ=255)
                                                                                                                                          ggggTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa   <  1:3545583/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAC  >  NZ_CP034943/112448‑112725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: