Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,034,593 C→A 16.1% intergenic (‑259/‑135) EO946_RS10175 ← / → EO946_RS10180 UPF0715 family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,034,5930CA16.1% 453.9 / 29.2 220intergenic (‑259/‑135)EO946_RS10175/EO946_RS10180UPF0715 family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (142/36);  new base A (2/40);  total (144/76)
Fisher's exact test for biased strand distribution p-value = 2.48e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

TTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATG  >  NZ_CP034943/2034454‑2034730
                                                                                                                                            |                                                                                                                                          
tttAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTc                                                                                                                                            >  2:1105705/1‑140 (MQ=255)
tttAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTc                                                                                                                                            >  2:2201324/1‑140 (MQ=255)
   aaTGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCCAACGTTTTGTACAGTGTTTCCCAGCTGTAGTCAGCTTCTTCATATACGACGTGTGCTAATATACTGTTTCaaa                                                                                                                                         >  2:3114404/1‑140 (MQ=255)
   aaTGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTGCATATACGAAGTGTGCTAATAAAATGTTTCaaa                                                                                                                                         >  2:495322/1‑140 (MQ=255)
         ggCTTTTTTGTTTACATGCATTTCCCTTCCCTAACCTGTCCTTATCGCCTTTTATTTTCAAAATTTTTTTTACTGTGTTTCCCAGCTTTAGTACGCTTTTTCATATACGATTTGTGATAATAAATTGTTTCAAAAACGAc                                                                                                                                  <  2:2377696/140‑1 (MQ=255)
         ggCTTTTATTTTTACCTGCTTATTCTTCCCCTAACCTCTCTTTATCACCGATTCTTTTTCAAACGTTTTGTAACGTTTTTCCACGATTAAGTCAGATTATTAATATACTCAGTGTGCTAATAAATTGTTAAAAAAACGAc                                                                                                                                  <  2:1357839/140‑1 (MQ=255)
          gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGaac                                                                                                                                 >  1:5406812/1‑138 (MQ=255)
          gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                 >  1:1703348/1‑140 (MQ=255)
          gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                 >  1:5664820/1‑140 (MQ=255)
          gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                 >  1:604986/1‑140 (MQ=255)
          gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                 >  1:1436668/1‑140 (MQ=255)
          gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                 >  2:3880265/1‑140 (MQ=255)
          gCTGTTATGTTTACATGCAGATTAATTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAATCGTTTTGTAAAGTGTTTAGCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATTTTTCTAAAACGaac                                                                                                                                 >  2:1436923/1‑138 (MQ=255)
               tATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAATCGACCGGGGt                                                                                                                            >  2:2577917/1‑140 (MQ=255)
                      aCATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGcct                                                                                                                     >  1:1826183/1‑138 (MQ=255)
                      aCATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTg                                                                                                                     >  1:389008/1‑140 (MQ=255)
                       attGCAGATTCCTTCCCTCCCCTGTCCTTATGTCCGTTTATTTTTCAAACTTTTTGTACAGTTTTTCCAAGCTTTAGTCTGCTTCTTCATATACTATTTGTGATAATAAAATTTTTCAAAAACGACCGGGGTAAAGCTGa                                                                                                                    <  2:5481620/138‑1 (MQ=255)
                        aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAGCGGGGTAAAGCTGAc                                                                                                                   >  2:3026138/1‑140 (MQ=255)
                        aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTCAAGCTGAc                                                                                                                   >  2:4797125/1‑140 (MQ=255)
                           cAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActct                                                                                                                >  2:2138059/1‑140 (MQ=255)
                             gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                              >  1:38109/1‑140 (MQ=255)
                                  cttccCTACCCTGACATTATATCCGTTAATTTTAAAACCTTTTTTTACTTTTTTACCCCCCTTTAGACTGATTGTACATATACCATGTTTGATAATAAATTTTTTAAAACACGAACGGGGTAAAGCTGACTCTCTTGTCt                                                                                                         <  2:2652783/140‑1 (MQ=255)
                                        tAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATTTTTCAAAAACGACCGGGGTAAATCTGACTCTCTTGTCTTAATgg                                                                                                   >  1:1897456/1‑140 (MQ=255)
                                        tAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTGTAGTCAGCTTCTTCAGATACGAAGTGTGCTAATAAAATGTTTCAAAAAAGACCGGGGTAAAGCTGACTCTCTTGTCTTAATgg                                                                                                   >  1:771616/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTCATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  1:4079578/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTTTATTAATGGGAAagag                                                                                            >  2:3588619/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  1:3676822/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  2:12483/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  1:3938093/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  1:3293462/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  1:5622253/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                            >  2:3731137/1‑140 (MQ=255)
                                               tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTGCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAACGCTGACTCTCTTGTCTTAATGGGCAagag                                                                                            >  1:1464463/1‑140 (MQ=255)
                                                    aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTACTAAAATGGTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGGCTTAATGGGAAAGAGTCAAc                                                                                       >  1:3139442/1‑140 (MQ=255)
                                                         cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                  >  1:5254098/1‑140 (MQ=255)
                                                         cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                  >  1:1359757/1‑140 (MQ=255)
                                                         cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                  >  1:2658775/1‑140 (MQ=255)
                                                         cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGGGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                  >  2:2486231/1‑140 (MQ=255)
                                                          tattttttttCAACCTTTTTGTACCGTTTTTCCCCTCTTTTGTCAGTTTCTTAATATACTATGTTTGATAATAAATTGTTTCAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaaa                                                                                 <  2:407006/135‑1 (MQ=255)
                                                          gCTTATTTTTAAAACTTTTTTAACTTTGTTTCCCAGCTTTAGTCAGGTTCTTAATACAGGATGTGTTCTAATAAACTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaaa                                                                                 <  2:2703722/140‑1 (MQ=255)
                                                           cttcttTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATAAGAAGTGTGGTAATAAAATGTTTCATAATCGACCGGGGTAAAGCTGTCTCTCTTGTCTTATTGGGAAAGAGTCAACGaaaaaa                                                                                >  1:3366459/1‑140 (MQ=255)
                                                               tttttAAAAGTTTTTTTACTGTTTTCCCCAGTTTTAGTCAGTTTATTAATAAACTATGTTTGATAATAAAATGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCg                                                                            <  2:4890109/140‑1 (MQ=255)
                                                                      acctttttttACTGTGTTTCCCACCTTTATTCTGCTAAGTCATAAACGATGGTTGATAATAAAATGTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCgaatgaat                                                                     <  2:1440314/136‑1 (MQ=255)
                                                                       aCGTTTTGGTACCTTTTTTCCCAGCTTTCTACAGGTTATTCATAACCGAGGTGTCCTAAAAAAATTTAAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCgaatgaat                                                                     <  1:4744561/140‑1 (MQ=255)
                                                                        cGTTTTTTACAGGGTTCCCCCGCTTTATTCAGCTTCTTCATATACTAAGTGTGATAATAAAATTTTACAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                   <  2:4695524/140‑1 (MQ=255)
                                                                        cGTTTTGTACGGTTGTGCCCATGTTTAGTCCGCTTTTTATTATACGTATTGTGCTAATAAATTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                   <  1:2183148/140‑1 (MQ=255)
                                                                        cGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                   >  1:1001081/1‑140 (MQ=255)
                                                                          ttttGTACTTTGTTTCCCCGTTTTTTTCTGTTTCTTCATATCCGATGTGTGCTAATAAAATTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCt                                                                 <  2:1968699/140‑1 (MQ=255)
                                                                          ttttGTACTGTGTTCCCCAGTTTTTTTCATCTTATAAATAAACTATGTGTGAAAAAAAAATTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCt                                                                 <  1:3029456/140‑1 (MQ=255)
                                                                                   tgtttttCCCAGTTTTTGTCCGTTTCTTCATAACCGATGTGTTAAAAAAAATTTTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAaca                                                         <  1:5057322/136‑1 (MQ=255)
                                                                                    gtgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacag                                                        >  1:2850831/1‑140 (MQ=255)
                                                                                    gtgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacag                                                        >  1:1284381/1‑140 (MQ=255)
                                                                                    gtgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacag                                                        >  1:3568794/1‑140 (MQ=255)
                                                                                     tttttCCCAGTTTTCTTCAGTTTCTTAATATACTATGTTTGAAAATAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       <  2:1748843/138‑1 (MQ=255)
                                                                                     tgtTTCCCTTTTTTAGTCAGCTTCTACATATACGATGTGTGATAATAAAATTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       <  1:1256060/140‑1 (MQ=255)
                                                                                     tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAATTGTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       <  2:3852773/140‑1 (MQ=255)
                                                                                     tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       >  2:3949418/1‑140 (MQ=255)
                                                                                     tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       >  1:1968699/1‑140 (MQ=255)
                                                                                     tgtTTCCATGTTTTATTCAGCTTCTTATTATACGTCGTTTTATAATAAAATGTTACAAACACGACCGGTGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       <  1:2469424/140‑1 (MQ=255)
                                                                                     tgtTGCCCCGGTTTCGTCCGGTTTTTCATATACGATGTGTGATAATAAAATGTTTCAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                       <  2:3552340/140‑1 (MQ=255)
                                                                                       tttCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagaca                                                     >  2:4339229/1‑140 (MQ=255)
                                                                                       tttCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagaca                                                     >  2:957361/1‑140 (MQ=255)
                                                                                       tttCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagaca                                                     >  2:4316353/1‑140 (MQ=255)
                                                                                          cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGaa                                                  >  1:4322706/1‑140 (MQ=255)
                                                                                          cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGaa                                                  >  2:437240/1‑140 (MQ=255)
                                                                                              cttttAGTCAGCTTCTTCATATACCAAGTGTGATAATAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaag                                              <  2:1930661/138‑1 (MQ=255)
                                                                                               cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                             >  1:1219757/1‑140 (MQ=255)
                                                                                               cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                             >  2:814085/1‑140 (MQ=255)
                                                                                               cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                             >  1:4141291/1‑140 (MQ=255)
                                                                                                 ttcctCAGCTTCTTCTAATACGATGTGTGCTAATAAAATGTTTAAAAAACGACCGGGGTAAAGCTGACTCTATTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaagaag                                           <  1:5742753/136‑1 (MQ=255)
                                                                                                 ttAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAACAAAAGCGAATGAATACCTGCCTAACAGAGAGAATaagaag                                           >  1:5380111/1‑140 (MQ=255)
                                                                                                 ttAGACAGCTTCTTCATATACGATTTGTCCTAATAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaagaag                                           <  2:2726888/140‑1 (MQ=255)
                                                                                                   aGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAagag                                         >  2:4524566/1‑140 (MQ=255)
                                                                                                      aagattcttcGAAGACGAAGTGTGATAATAAATTTTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTg                                      <  2:5327683/136‑1 (MQ=255)
                                                                                                         cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata                                   >  1:1633357/1‑140 (MQ=255)
                                                                                                         cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata                                   >  2:4133064/1‑140 (MQ=255)
                                                                                                         cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata                                   >  2:5579367/1‑140 (MQ=255)
                                                                                                              tcATATCCCAAGTGTGATAATAAAATGTTTCAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCaa                              <  2:3972399/140‑1 (MQ=255)
                                                                                                                   taCGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACtt                         >  2:2464437/1‑140 (MQ=255)
                                                                                                                   taCGAAGTGTCCTCATAAAATGTTTCAAAAACGAACGGGGTAAAGCTGACTCTCTTGTCTAAATGGGAAAGAGTCAACGAAAAAAATCTAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACtt                         >  1:993170/1‑140 (MQ=255)
                                                                                                                    tctaaGTGTGCTAATAAAATTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACttt                        <  2:1197505/137‑1 (MQ=255)
                                                                                                                    ccGAAGTTTGATAATAAATTTTTAAAAAAACGACCGGGGTAAATCTGACTCTGTTGTCTTAATGTGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACttt                        <  2:1706895/139‑1 (MQ=255)
                                                                                                                    actatttgttCTAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACttt                        <  2:3764735/134‑1 (MQ=255)
                                                                                                                       aaGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAGTGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCg                     >  2:5677491/1‑140 (MQ=255)
                                                                                                                       aaGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACttttag                     >  2:1124263/1‑138 (MQ=255)
                                                                                                                          tgtgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACACAATAAGAAGAGTTGATATTCAACACTTTTCGtta                  >  2:199167/1‑138 (MQ=255)
                                                                                                                            ttcTAATAAAATGTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGACTGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATCTTCAACACTGTTGGTACAg                >  2:3305751/3‑140 (MQ=255)
                                                                                                                            tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTACAg                >  1:5173247/1‑140 (MQ=255)
                                                                                                                            tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                >  2:4265848/1‑140 (MQ=255)
                                                                                                                            tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                >  2:1577128/1‑140 (MQ=255)
                                                                                                                            tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                >  2:5154076/1‑140 (MQ=255)
                                                                                                                            tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                >  1:4025802/1‑140 (MQ=255)
                                                                                                                            tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                >  2:2892035/1‑140 (MQ=255)
                                                                                                                               taataaAATGTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg             <  2:3801755/140‑1 (MQ=255)
                                                                                                                               taaaaaAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCGGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg             <  2:3249053/140‑1 (MQ=255)
                                                                                                                                  acaaaTCTTTAACACACGCCGGGGGTAAAGCTGACTATCTTGTCGTAATGGGAAATACTCAACGAAAAAAAGAGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGa          <  2:712325/138‑1 (MQ=255)
                                                                                                                                   taatcttttCAAAACACGACCGGGGTAACGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGa          <  2:3625520/134‑1 (MQ=255)
                                                                                                                                    aaaTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGaca        >  1:4616016/1‑140 (MQ=255)
                                                                                                                                    aaaTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGaca        >  1:2197921/1‑140 (MQ=255)
                                                                                                                                      aTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACATACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGGGACAcc      >  2:3188963/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATG  >  NZ_CP034943/2034454‑2034730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: