Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,139,480 A→C 7.3% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,139,4800AC 7.3% 1078.6 / 10.7 420A119A (GCT→GCGdeoDpurine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (257/109);  new base C (3/51);  total (260/160)
Fisher's exact test for biased strand distribution p-value = 1.12e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 434 total aligned reads displayed.

TTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATT  >  NZ_CP034943/2139342‑2139617
                                                                                                                                          |                                                                                                                                         
  gCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCg                                                                                                                                        >  1:5614674/1‑140 (MQ=255)
   ctaaTCGTCGTTTTAGAGTTGATCAGCTTTAAATACGCTTCCTACCGTCACCGGCACGCGTTTATCCTTTGGGGAATCATAGGAATTTTTTAAAAGCTCTAAATCTGCGAAAGGCGAAAAATCAATACTACCGAAAGCGa                                                                                                                                       <  2:1719025/138‑1 (MQ=255)
                  gAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACCTCCGAAAGCGACTCTGTTCATTtgtg                                                                                                                        >  1:761765/1‑140 (MQ=255)
                     cttaTCAGCGGTAAATCCGCTTCCTACCGTCCCCGGCACGCCTTTCTCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCGGCGAAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                     <  2:1431689/137‑1 (MQ=255)
                     cggATCAGCTGTAAATACGGTTCGTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCACTAAATCTGCGCAAGGCGCAAAATCAATACTACCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                     <  2:4727421/138‑1 (MQ=255)
                     cTGATCAGCTGTAAATCCGCTACCTACCGTCACCGGCACCCCTTAATCCTTTGCGGCATCATAGGCATTTTTTAAACGCTCTAAATCTGCGCAAGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                     <  2:1159318/140‑1 (MQ=255)
                     cTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                     >  1:903307/1‑140 (MQ=255)
                     cTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCCCAAAATCAATACTTCCGAAAGCGACACTGTTCATTTGTGAAt                                                                                                                     >  1:3478489/1‑140 (MQ=255)
                      tGATCCGCTGTAAAAACGATTACTACCCTAACCGCCACCCCTTTATCCTGTGCGCAATAAAAGTAATTTTTTAAAAGACCGAAATCTGCTCAAGGCGAAAAACCAATACTTCCGAAACCGACTCTGTTCATTTGTGAATc                                                                                                                    <  2:223552/140‑1 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATCCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:1950076/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAtacg                                                                                                                  >  2:3330922/1‑137 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:4806447/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:782689/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:1013826/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:3854370/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:3562833/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:2695118/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:428111/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:1663545/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:4949349/1‑140 (MQ=255)
                        aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTATGCGCCATCATAGGCAATTTTTAAAAGCACGAAATCTGAGCAATGCGCAAAATCAATACTACAGAAAGCAACTCAGTTCATATGAGAATCAg                                                                                                                  >  1:5561415/1‑140 (MQ=255)
                             cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCGTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGACTCAGTTGAg                                                                                                             >  2:3724132/1‑140 (MQ=255)
                             cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCACGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                                             >  2:3218300/1‑140 (MQ=255)
                                tAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGATATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGAt                                                                                                          >  2:582508/1‑140 (MQ=255)
                                tAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGAt                                                                                                          >  2:5010884/1‑140 (MQ=255)
                                tAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCAGTTGTGAATCAGTTGAGGAt                                                                                                          >  1:3708350/1‑140 (MQ=255)
                                  aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGt                                                                                                        >  2:2648792/1‑140 (MQ=255)
                                  aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGt                                                                                                        >  2:5710189/1‑140 (MQ=255)
                                     aCGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAt                                                                                                     <  2:624348/140‑1 (MQ=255)
                                       gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  1:4647895/1‑140 (MQ=255)
                                       gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  2:2050825/1‑140 (MQ=255)
                                        cTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGTGGATGTCATAGc                                                                                                  >  2:2821948/1‑140 (MQ=255)
                                        cTTCATACCGTCACCGGCACGCCTTTAACCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGc                                                                                                  <  2:4083684/140‑1 (MQ=255)
                                          tcctccCGTCACCGGACCCCCTTTATCCTTTCCGGCATAATAGGAATTTTTAAAACCCCCCAAATCGGCCAAAGGCCAAAAAAAAATACTCCCAAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                <  1:3607592/140‑1 (MQ=255)
                                          tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  1:1217379/1‑140 (MQ=255)
                                          tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  2:1290374/1‑140 (MQ=255)
                                          tCCTACCGTCACCGACACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  2:4116531/1‑140 (MQ=255)
                                           ccTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTTAATCAGTTGAGGATGTCATAGCCaa                                                                                               >  2:3420827/1‑140 (MQ=255)
                                                   cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                       >  1:423894/1‑140 (MQ=255)
                                                     ccGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                     >  2:3996096/1‑140 (MQ=255)
                                                     ccGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                     >  2:120855/1‑140 (MQ=255)
                                                               tttATCGTTAGCGCCACAAAAGGCATTTTTTAAAACCACGAACGCGCCGCACGCCGAAAAATCACTACTCCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGa                                                                           <  1:3771426/140‑1 (MQ=255)
                                                                 tATCCTTTGCGGCATCATAGGATTTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAAGACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                         <  1:5297661/140‑1 (MQ=255)
                                                                 tATCCTTTGCGCCAAAATTGGCTTTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAAAACTGCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                         <  1:3135884/140‑1 (MQ=255)
                                                                       ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                                   >  2:1862415/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGtt                                                                                                                <  2:182590/94‑1 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGtt                                                                                                                >  1:182590/1‑94 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCCTAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:4010973/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:2187853/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:5493192/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:1426308/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:2832577/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:148703/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:2564554/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:5218713/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:5037676/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:192537/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:4107364/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:4754849/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:4250865/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  2:802573/1‑140 (MQ=255)
                                                                        tGCGGCATCATAGGCATTTTTTAAAAGCTCCAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                            <  2:1989039/98‑1 (MQ=255)
                                                                          cGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCgg                                                                >  1:1028700/1‑140 (MQ=255)
                                                                          cGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCgg                                                                >  1:2440075/1‑140 (MQ=255)
                                                                            gCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGAt                                                              >  1:513454/1‑140 (MQ=255)
                                                                               tcatCGGAATTTTTCAAAAGCACCCAATCTGCTCACGGCGCAAAATCAATACTTCCTAACGCGACTCTGTTCATTTGTGACTCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACAGCTTTGCGGATAgc                                                           <  2:1075303/140‑1 (MQ=255)
                                                                                     gaaTTTTTTAAATGCTCGAAATCTGCGCATGGCGAAAAATCAATAGTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:1586510/138‑1 (MQ=255)
                                                                                     gaaTTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:3420813/138‑1 (MQ=255)
                                                                                     gaaTTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATCCTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:2751072/138‑1 (MQ=255)
                                                                                     gaaTTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATAAATACTGCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  2:3800128/138‑1 (MQ=255)
                                                                                     gaaTTTTTTAAAAGCTCCAAATCGGCCCAAGGCGCAAAACCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:3258572/138‑1 (MQ=255)
                                                                                     gCATTTTTTAAAAGCGCGAAATCGGCGCACGGCGAAAAATCAATACTCCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:1991899/140‑1 (MQ=255)
                                                                                         tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGGTGTCATAGCCAAAATGACGTGCCGCACTTTGTCATCTTTGCGGTGAGCGGCGCAAGag                                                 >  1:4496755/1‑139 (MQ=255)
                                                                                         tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGaa                                                 >  2:4591463/1‑140 (MQ=255)
                                                                                             tAAAAGCTCGAAATCTGCGCCCGGCGAAAAAAAAATCCTCCCTAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                                             <  1:1265056/140‑1 (MQ=255)
                                                                                              aacagatCGAAATCTGCGCAAGGCGAAAAATAAAGACTTCCTAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAc                                            <  1:2922209/134‑1 (MQ=255)
                                                                                              aaaaGCTATAAATCTGCGCAAGGCGAAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAc                                            <  1:4341929/140‑1 (MQ=255)
                                                                                                     cGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                     <  1:5710189/140‑1 (MQ=255)
                                                                                                              cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCCAGAACCTACTCTTATTAGATTTTGc                            >  2:2751363/1‑140 (MQ=255)
                                                                                                               gcACGGCGAAAAATCAATACTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  1:1716565/140‑1 (MQ=255)
                                                                                                               gaaaGGCGCAAAATCAATCCTACCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  2:2502373/138‑1 (MQ=255)
                                                                                                               gaaaGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  1:5513225/138‑1 (MQ=255)
                                                                                                                  aGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACAt                        >  1:4944448/1‑140 (MQ=255)
                                                                                                                   ggCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATc                       >  2:3151876/1‑140 (MQ=255)
                                                                                                                     cgcAAAATCAATCCTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGt                     <  2:1463625/140‑1 (MQ=255)
                                                                                                                      gcAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTa                    >  2:955802/1‑140 (MQ=255)
                                                                                                                      gcAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTa                    >  2:4424048/1‑140 (MQ=255)
                                                                                                                      gcAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTa                    >  2:3047412/1‑140 (MQ=255)
                                                                                                                         aaaTCAATCCTACCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                 <  2:3478489/140‑1 (MQ=255)
                                                                                                                          aaTCAAGACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCACAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCtt                <  2:2900517/140‑1 (MQ=255)
                                                                                                                          aaTCAAAACTTCCTAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCtt                <  2:884703/140‑1 (MQ=255)
                                                                                                                              actACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa            <  2:4307546/138‑1 (MQ=255)
                                                                                                                              actACTGCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTGTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa            <  2:4790205/138‑1 (MQ=255)
                                                                                                                              aaTACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa            <  2:5498792/140‑1 (MQ=255)
                                                                                                                                    tCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaattaatt      <  1:603772/140‑1 (MQ=255)
                                                                                                                                     ccGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattc     >  1:4300462/1‑140 (MQ=255)
                                                                                                                                      ccaaaGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattca    <  2:3854370/138‑1 (MQ=255)
                                                                                                                                      cGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattca    <  2:5197493/140‑1 (MQ=255)
                                                                                                                                       gAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                                             >  2:1937063/1‑98 (MQ=255)
                                                                                                                                        aaaGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattcatt  <  2:766197/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TTGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATT  >  NZ_CP034943/2139342‑2139617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: