BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,528	G→T	8.7%	Q57K (CAA→AAA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,528	0	G	T	8.7%	806.6 / 17.6	347	Q57K (CAA→AAA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base G (61/243); new base T (33/9); total (94/253)
Fisher's exact test for biased strand distribution p-value = 1.11e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

CCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTA  >  NZ_CP034943/2214389‑2214665

ccgcctTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTg                                                                                                                                           <  2:5416574/140‑1 (MQ=255)

      tAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTaaaaaa                                                                                                                                     >  2:389460/1‑140 (MQ=255)

       aaTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaataat                                                                                                                                    <  1:139158/140‑1 (MQ=255)

        aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATg                                                                                                                                   >  2:5487881/1‑140 (MQ=255)

        aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATg                                                                                                                                   >  2:3483825/1‑140 (MQ=255)

        aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATg                                                                                                                                   >  2:4032786/1‑140 (MQ=255)

        aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTGCCGGCCGTTTATATTTTTTAaataatt                                                                                                                                   >  2:2012008/1‑139 (MQ=255)

           ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGttt                                                                                                                                <  2:2855076/140‑1 (MQ=255)

                taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTc                                                                                                                           <  2:1601999/140‑1 (MQ=255)

                   tccttttGCTCAGTTTTTGGATGTTTTTCTCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  2:4616175/137‑1 (MQ=255)

                   taaTTTTGCTCTGTTTTTGGATGTTTTTCGCTTTATTACTATACAAACCGATTGATTTAATATCCTGCTGATGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGTCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  2:362605/138‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGTATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  2:4886814/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACCAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATGTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:4037204/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAAtttttttttaaat                                                                                                                        >  2:2550113/1‑133 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:1577746/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:1469994/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:4246864/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  2:3732906/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:5377205/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:2629159/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:5679818/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  2:452834/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        >  2:2616289/1‑140 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:580491/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGCTTTTGTCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGAGAAACAGCCAAATAGTCTTCCGGAGGTTTATATGTTTTAAATTATGTTTTGGTCagt                                                                                                                        <  2:234462/140‑3 (MQ=255)

                    cATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCActc                                                                                                                       <  1:743372/140‑1 (MQ=255)

                        ttGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGtt                                                                                                                   <  2:1115476/140‑1 (MQ=255)

                              agattttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAGATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                             <  1:2167023/137‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATTTTTTTGTGACTCTGTTTaaaaa                                                                                                             >  1:5199993/1‑139 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTCCGATACAAACCGATTGATTTACTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  1:3812549/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  2:4751223/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  2:4980457/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  2:3218806/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  1:5414869/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  2:4286250/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                           <  2:3777733/140‑1 (MQ=255)

                                   ttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCAt                                                                                                        <  1:1172751/140‑1 (MQ=255)

                                     ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                      <  1:5080717/140‑1 (MQ=255)

                                     ggATGTTTGTCGCTTTATTACGATACACACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                      <  1:3469132/140‑1 (MQ=255)

                                        tGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTa                                                                                                   <  1:1591977/140‑1 (MQ=255)

                                          tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCACATAGTCTTCCGGCCGTTTATATTTTTGAAATAATTGTTTGTTCACTATGTTTACAAGTGCAACAGTACa                                                                                                 >  2:3565798/1‑140 (MQ=255)

                                          tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATTAATTTTTGTTGAAGGTGTTTACAAGTGGATATTTACa                                                                                                 >  1:2072016/1‑140 (MQ=255)

                                          tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTGAAATAATGTTTTTGTGACTCTGTTTTAAAGTGGATCTGTaac                                                                                                 >  1:2306285/1‑138 (MQ=255)

                                             ttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTg                                                                                              <  2:3703911/140‑1 (MQ=255)

                                               cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                            <  2:2240665/140‑1 (MQ=255)

                                               cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                            <  2:1334547/140‑1 (MQ=255)

                                               cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                            <  1:5130466/140‑1 (MQ=255)

                                               cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                            <  1:3769716/140‑1 (MQ=255)

                                                    tattaCGATACAAACGGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTTTTTACAAGTGCATCTGTACATTGCGCAGAt                                                                                       <  1:2430869/140‑1 (MQ=255)

                                                          gATACAAACCTATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTAAATTGCGCAGATAAAGCg                                                                                 <  1:18574/140‑1 (MQ=255)

                                                            taaaaaCCTATTGATTTAATATCCTGCTGAAGCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGAc                                                                               <  1:4499056/137‑1 (MQ=255)

                                                            tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                        <  1:3079971/99‑1 (MQ=255)

                                                            tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                             >  2:3807728/1‑110 (MQ=255)

                                                            tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTAAATAATGTTTTTGTGACAATGTTTTAAAATTCATATGTAAAATGCGCAGATAAATCGAc                                                                               >  1:3566227/1‑140 (MQ=255)

                                                                 aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTAGGTTTAAAAGTGCATATTTACATTGCGCAGATAAAGCGACTGCTa                                                                          >  2:2425540/1‑140 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATTTTTTTGTGACTCTGTTTACAAGTTCATATGTACATTGCGCAGATAAAGCGACTGCTACCACt                                                                     >  2:157463/1‑140 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAAAGTTTTTGTCACACTGTTTAAAAGTGCATATGTACATTTCGCAGATAAACCGACTGCTACCaaa                                                                     >  1:4724606/1‑138 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTTAATAAATTTTTTGTCACACTTTTTAAAACTGAATATGTACAATTCGCAGATAAAGCCAATGCTACAAca                                                                     >  1:275269/1‑139 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTGAAATAATGTTTTTGTGACTCTGTTTAAAAGTTGATATGTAAAATTGGCAGATAAAACGACCGCTAacaca                                                                     >  1:4833621/1‑139 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATTTTTTTGTGAAAATGTTTACAAGTTCATCTGTACAATGCGCAGATAAAGCGACTGCTACCAca                                                                     >  1:2623941/1‑139 (MQ=255)

                                                                             aaTATCCTGCTGAAGCTCTTCCCGCGAAACTGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                              <  1:2826061/140‑1 (MQ=255)

                                                                             aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATGTGTAAATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                              <  1:3265084/140‑1 (MQ=255)

                                                                             aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGAAGATAAAGCGACTGCTACCACTAATTCaa                                                              <  1:5561221/140‑1 (MQ=255)

                                                                                   ctgcttAAGCTTGTCCAGCGAAACTGACAAATAGTCTTGGGGCCGTTTATCTTTTTGATATAATGTTTTGGTCACTCTTTTTACAAGTGCATCTGTCCATTGCGCCGTTACAGCGACTGCTACCACTAATTCAAAAGGAt                                                        <  1:1895381/140‑1 (MQ=255)

                                                                                   ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATATTGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                        <  1:837552/140‑1 (MQ=255)

                                                                                   ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTTGTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACAAATTCAAAAGGAt                                                        >  2:4403551/1‑140 (MQ=255)

                                                                                   ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                        <  1:1785135/140‑1 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCGAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTTGTCACTCTGTTTACAAGTGCATGTTTACATTGCGGAGTTTAATCGACTGCTAGCACTAATTAAAAATGAtt                                                       >  2:3404401/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAATAATTTTTTGGTGACTCTGTTTAAAAGTGCATCTTTAAATTTCGCACATAAAACGACTGCTAACACAAAAAAAAAAAGAtt                                                       >  1:5045185/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGAGCAGATAAAGCGACTGCTAACACAAATTCAAAAGGAtt                                                       >  2:5548196/1‑140 (MQ=255)

                                                                                       tgAAGCTCTTCCAGCGAAACAGCAAAATATTATTCCGGCGGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGa                                                    <  1:4566740/140‑1 (MQ=255)

                                                                                        gAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                   <  2:1878665/140‑1 (MQ=255)

                                                                                        gAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTGGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                   <  2:101435/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACCGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAAGTCAAAAGGATTGGAATGaa                                               <  2:1040185/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                               <  1:3346155/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCAAAATATTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                               <  1:2384408/140‑1 (MQ=255)

                                                                                            ctctCCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                               <  1:5628630/140‑1 (MQ=255)

                                                                                              ctTCCAGCGAAACCGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                             <  1:4435385/140‑1 (MQ=255)

                                                                                              ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                             <  2:4649429/140‑1 (MQ=255)

                                                                                              cgtCCAGCGAAACAGCCAAATAGTCTTGCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                             <  2:5261456/138‑1 (MQ=255)

                                                                                                tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATGTTTTGGTGACTCTGTTTTCAAGTGCATCTGTACATTGCGCAGATAAATCGGCTGCTAACACAAATTAAAAATGATTTGAATGAAACAg                                           >  1:4124158/1‑140 (MQ=255)

                                                                                                tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATTTTTTGTTGACTCTGTTTAACAGTGCATATGTACAATTCGCAGATAAAACGCCTGGTACCAAAAAATAAAAAGGATTGGTATTAAACaa                                           >  1:359621/1‑139 (MQ=255)

                                                                                                  cAGCGAAACCGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                         <  2:716045/140‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                         <  2:5401741/140‑1 (MQ=255)

                                                                                                      gAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTcaca                                     <  1:3003858/140‑1 (MQ=255)

                                                                                                        aaCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACAct                                   <  1:2517922/140‑1 (MQ=255)

                                                                                                         aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAATAATGTTTTGGTGACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAAATCAAAAGGATTGGAATGTACCAGTTCacacgc                                  >  2:803783/1‑138 (MQ=255)

                                                                                                         aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGACATAATGTTTTTGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCTATAAAGCGACTGCTAACCCGAATTCACAAGGATTGGAATGAAACAGTTcacaata                                  >  2:2964543/1‑137 (MQ=255)

                                                                                                            gCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                               <  2:5199993/140‑1 (MQ=255)

                                                                                                            gCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                               <  1:3142838/140‑1 (MQ=255)

                                                                                                                         ccGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTAACACTAATTCAAAAGGATTGGAATGAAACAGTTCACACTCTGCGTGTGGAAACATg                  >  2:5792768/1‑140 (MQ=255)

                                                                                                                             ccGTTTATACTTTTGAAATAATTTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc              <  1:2419906/140‑1 (MQ=255)

                                                                                                                               gTTTATATTTTTGAAATAATGTTTTGTTCACTATGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCt            <  2:1715565/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt          <  1:1043767/140‑1 (MQ=255)

                                                                                                                                 ttATATTTATGAAAGAATGTTTGGGTCACTCTGTTTACACGTGCAACTGTACATTGCGCAGATAAAGCGACTGCTACCACTCATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt          <  1:5282316/140‑1 (MQ=255)

                                                                                                                                 ctataTTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACAGTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt          <  2:1422837/139‑1 (MQ=255)

                                                                                                                                        tttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCt   <  1:2061082/140‑1 (MQ=255)

                                                                                                                                         ttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTa  >  1:1948032/1‑140 (MQ=255)

                                                                                                                                         ttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTa  >  2:3064479/1‑140 (MQ=255)

                                                                                                                                         ttGAAATAATGTTTTGGTCACACTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGTATAGGAATGAACCAGTTCACACTCTGCGAGTGGAAACATGTCGCCTATCTAGTCTa  >  1:3933615/1‑140 (MQ=255)

CCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTA  >  NZ_CP034943/2214389‑2214665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`