Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,408,718 G→C 7.8% P330A (CCG→GCG)  spoIIIAE ← stage III sporulation protein AE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,408,7180GC 7.8% 620.5 / 3.9 268P330A (CCG→GCG) spoIIIAEstage III sporulation protein AE
Reads supporting (aligned to +/- strand):  ref base G (108/112);  new base C (2/22);  total (110/158)
Fisher's exact test for biased strand distribution p-value = 6.74e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

GCGAGAGCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAA  >  NZ_CP034943/2408582‑2408856
                                                                                                                                        |                                                                                                                                          
gCGAGAGCTGCAAAAATATAAATGCCGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGaaa                                                                                                                                         <  1:3243905/140‑1 (MQ=255)
gCGAGAGCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGACTCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGaaa                                                                                                                                         <  2:3216403/140‑1 (MQ=255)
gCGAGAGCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGaaa                                                                                                                                         <  1:4417357/140‑1 (MQ=255)
gCGAGAGCTGCAAAAATATAAAGGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGaaa                                                                                                                                         <  1:2834772/140‑1 (MQ=255)
 cGAGAGCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGaaaa                                                                                                                                        <  1:3354567/140‑1 (MQ=255)
   agagCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGc                                                                                                                                      >  2:1139013/1‑140 (MQ=255)
   agagCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGc                                                                                                                                      >  1:2188138/1‑140 (MQ=255)
   agagCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGc                                                                                                                                      >  1:340066/1‑140 (MQ=255)
   agagCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGc                                                                                                                                      >  1:5007286/1‑140 (MQ=255)
     agCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCg                                                                                                                                    <  2:4655677/140‑1 (MQ=255)
      gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                   >  2:4168048/1‑140 (MQ=255)
      gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                   >  2:36711/1‑140 (MQ=255)
          cacaaaTATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               <  1:2057771/137‑1 (MQ=255)
          cAAAAATATAACTGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               <  1:3220700/140‑1 (MQ=255)
          cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               <  1:3564780/140‑1 (MQ=255)
          cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               >  1:2358529/1‑140 (MQ=255)
          cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               <  1:5599977/140‑1 (MQ=255)
          cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               >  1:5699210/1‑140 (MQ=255)
          cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               >  1:3120911/1‑140 (MQ=255)
          cAAAAATATAAATGACCCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               >  1:3378065/1‑140 (MQ=255)
                         cGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACa                                                                                                                             >  1:5570671/1‑127 (MQ=255)
                         cGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCg                                                                                                                >  2:2786442/1‑140 (MQ=255)
                         cGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCg                                                                                                                >  2:816798/1‑140 (MQ=255)
                          gCTTTTGCTGATGACTTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGa                                                                                                               >  1:4046934/1‑140 (MQ=255)
                              ttGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTCTAAATAAAAGCGAGGGACAGCACTTTGATTGCCGGAACAGCCGCAATACAGATCAAAATAGCGACTcc                                                                                                           >  1:5654022/1‑140 (MQ=255)
                                    ttgccGTCGCGGCAGTTAATGCCGGGCCCGCCTCCACGCGGCTGAAGAATCGCGGCTGCCAGTTTATAAATAAACGCGAGGGAAAGCACTTTGTTTGCCGCAAAACCCGAAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                     <  1:2472573/136‑1 (MQ=255)
                                    atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                     >  2:194106/1‑140 (MQ=255)
                                    atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGATTATAAATAAACGCGAGGGAAAGCACTTTTATTGCCGCAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                     <  2:4820596/140‑1 (MQ=255)
                                     agaCGTCGATGCAGTTAATGACGGGCCCGCCTCCAATCGGCTGAAGAATCGCGGCTGCCAGCTTAGAAAGAAAAGCGAGGTAAATCACTTTGATTGGCTGAAAAGACGAAATACAGATCAAAATACCGAATCCGAGCATc                                                                                                    >  1:4913685/2‑140 (MQ=255)
                                         gTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTa                                                                                                >  1:2409750/1‑140 (MQ=255)
                                           cGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACt                                                                                              >  2:4328232/1‑140 (MQ=255)
                                                   gTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAATCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAttttt                                                                                      >  1:1811124/1‑140 (MQ=255)
                                                    tAATGACGGGCCCGCCTCCACGCGGCTGAACAATCGCGGCTGCAAGCTTATAAACAAAAGCGAGGGAACGCACTTTGATTCCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                     <  2:4902995/140‑1 (MQ=255)
                                                    tAATGACGGGCCCGCCTCCAAGCGGCTGATGAATCGCGGCTGCCAGCTTATAAATAAACGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                     <  2:536525/140‑1 (MQ=255)
                                                    tAATGACGGGCCCGCCACCAAGCGGCTTAACAATGGCGGCTGCACGCTTATAAATAAACGCGCGGTAACGCACTTTGATTGCCGCAAACGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                     <  2:2518306/140‑1 (MQ=255)
                                                      aTGACGGCCCCCCCTCCACGCGGTTGAAGACGCGCGGCTGCCCGCTTATAAATAAACGCGAGGGAACGCACTTTCATTGCCGGAAACGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                   <  2:5053540/140‑1 (MQ=255)
                                                         cggggCCCGCCTCCCCGCGGCTGATGAATGGCGGGTGCCAGCTTTTAAATAAACGCGAGGTCACGCAATGTGATTGCGGGAAAAGCCGCAATACAGAGCATAATCGCGACTCCGAGAATCCCTACTGTATTTTTcagcag                                                                                <  2:1325490/138‑1 (MQ=255)
                                                         ccGGGCCCGCCTCCAAGCGGCTTAATAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcagcag                                                                                <  2:1347026/139‑1 (MQ=255)
                                                         aCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcctgtc                                                                                >  2:544677/1‑135 (MQ=255)
                                                             gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAACGCACTTTGATTGCCGGaaa                                                                                                                                         >  1:1785348/1‑79 (MQ=255)
                                                             gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                            >  1:2639137/1‑140 (MQ=255)
                                                             gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                            >  1:4736754/1‑140 (MQ=255)
                                                             gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATACCTACTGTATTTTTCAGCAGTAAg                                                                            >  2:4041999/1‑140 (MQ=255)
                                                                   cgccACGCGGCTGAAGACTCGCGGCTGCCCTCTTATAAATAAACGCGCGGGAAAGCACTTTGATTGCCGCAAACGCCGAATTACACAAAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                      <  1:3499843/138‑1 (MQ=255)
                                                                   cTCCAAGCGGATGAAGAATGGCGGCGGCCAGATTATAAATAAAACCGAGGAAAACCACTTTGATGGCCGCAAAAGCGCAAATAAAGATCAAAAAAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                      <  1:3831625/140‑1 (MQ=255)
                                                                     ccACGCGGCTGAAGAATCGCGGCTGCCATCTTATAAATAAAAGCGCGGCAAAGCACTTTGATTGCCGGAAAAGCCGAAATACTGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                    <  2:700579/140‑1 (MQ=255)
                                                                      cAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                               >  2:3491208/1‑80 (MQ=255)
                                                                        aGCGGCTTAATAATCGCGGCTCCAAGATTAAAAATAAATGCGAGTCAAAGAAATTTGATTGCCGGAAACGCCGAAATACTGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                 <  1:4473802/140‑1 (MQ=255)
                                                                        aGCGGCTTAAGAAGCGCGGCTGCCAGCTTATAAATACACCCCAGGGAAAGCAATTTGATGGCCGGAAAAGCCGCAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                 <  1:5511398/140‑1 (MQ=255)
                                                                         gCGGCTGACGATCCGCGGCTGCCATTTTATAAATAAAAGCGAGGGAACCAACTTTGATTGCCGGAAAAGCCCCAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATc                                                                <  1:1096651/140‑1 (MQ=255)
                                                                           ggCTTATGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGTAAAGCACTTTGATTGCCTGAAAAGCGGAATTACACATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                              <  2:1505475/140‑1 (MQ=255)
                                                                           ggCTGACGAATCGCGGCTGCAAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                              <  2:3848016/140‑1 (MQ=255)
                                                                           ggCTGAAGAATCGCGGCTGCCAGATTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                              <  2:2879937/140‑1 (MQ=255)
                                                                            gCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGaaa                                                                                                                                         >  2:1807998/1‑64 (MQ=255)
                                                                             cTGAAGAATCGCTGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCAGAGAATCCGTACTGTATTTTTCAGCAGTAAGGAAGCGCTCATcacat                                                            >  1:2401561/1‑139 (MQ=255)
                                                                             cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCACAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                            >  1:3465697/1‑140 (MQ=255)
                                                                             cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                            >  1:1516029/1‑140 (MQ=255)
                                                                             cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                            >  1:5435741/1‑140 (MQ=255)
                                                                                    aTCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGt                                                     <  2:5647071/140‑1 (MQ=255)
                                                                                     tCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTc                                                    >  2:707614/1‑140 (MQ=255)
                                                                                      cggcgCTGCCAGCTTATAAATAAAAGCGAGGGAAATCACTTTCATTGCCGTAAAAGCCCCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                   <  2:4860700/136‑1 (MQ=255)
                                                                                      cgcgGCTGCCAGCTTATAAATAAACGCGAGGGAAAGCACTTTGCTTCCCGGAAACCCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                   <  2:2155950/140‑1 (MQ=255)
                                                                                      cgcgGCTGCCAGATTATAAAAAACCGCGTGGCAAAGCACTTTGTTTGCCGCAAACGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                   <  1:816872/140‑1 (MQ=255)
                                                                                      cgcgGCTGCCAGATTAAAAATAAAAGCGAGGCAAAGCACTTTTATTGCCGCAAAAGCCGAAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                   <  2:2191704/140‑1 (MQ=255)
                                                                                      cgcgGCGGCCAGCTTATAATCAAAAGCTAGGGAAAGCACTTTTTTGCCCGAAAAAGCCGCAATCCTGACCAAAACAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                   <  1:3776485/140‑1 (MQ=255)
                                                                                       gcgGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcgc                                                  >  1:4920320/1‑140 (MQ=255)
                                                                                             gCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGt                                            >  2:2017013/1‑140 (MQ=255)
                                                                                             gCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGt                                            >  2:184199/1‑140 (MQ=255)
                                                                                             gCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGt                                            >  2:1648123/1‑140 (MQ=255)
                                                                                              ccAGTTTATAAAAAAAAGCGCGGGAAAGCACTTTTATTGCCGTAAAAGCCGAAATACAGATAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTa                                           <  1:5399214/140‑1 (MQ=255)
                                                                                              ccAGCTTATAAATAAACGCGAGGGAACGCACTTTGATTGCCGGAAAAGCCGCAATAAAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTa                                           <  1:4601837/140‑1 (MQ=255)
                                                                                              caatcTTATAAAAAAACGCGCGGTAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTa                                           <  1:4347477/136‑1 (MQ=255)
                                                                                                  cTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACa                                       <  2:5487252/140‑1 (MQ=255)
                                                                                                    tataAATAAAAGCGAGGGAAAGCAATTTGATTCCCGGAAAAGCCGCAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACAtt                                     <  1:5315367/140‑1 (MQ=255)
                                                                                                         atcaacgCGAGGCAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTAATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                <  2:1771467/134‑1 (MQ=255)
                                                                                                         ataaaCGGGTGGTAACGCACTTTTTTGGCCGTAACAGCCGAAATAAAGATAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                <  1:677319/140‑1 (MQ=255)
                                                                                                         ataaaAGCGAGGGAACGCAGTTTGATTGCCGGAAAACCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                <  2:2441374/140‑1 (MQ=255)
                                                                                                         ataaaAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                <  2:5301610/140‑1 (MQ=255)
                                                                                                         ataaaAGCGAGGGAAAGAAGTTTGATTGCCGGAAAAGCCGAAATACACATAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                <  1:1668126/140‑1 (MQ=255)
                                                                                                              cgCGAGGGAAAGCACTTTGTTTGCCGGAAACGCCGAAATACACATAAAAAACGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                           <  1:2965475/139‑1 (MQ=255)
                                                                                                              cgCGAGGCAACGAATTTTGATTCCCGCAAATGCCGAAATACAGATCAAAAGCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                           <  1:909233/139‑1 (MQ=255)
                                                                                                              aGCGAGGTAACCCATTTTGATTGCCGGAAAAGCCGAAATACAGAAAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                           <  1:2863443/140‑1 (MQ=255)
                                                                                                              aGCGAGGGAAAGCACTTTGATTGCCGTAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                           <  2:4126532/140‑1 (MQ=255)
                                                                                                                cGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACa                         <  2:38963/140‑1 (MQ=255)
                                                                                                                 gAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                >  1:1052238/1‑132 (MQ=255)
                                                                                                                  aGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAgg                       >  1:126998/1‑140 (MQ=255)
                                                                                                                        aGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATaaa                 >  1:797678/1‑140 (MQ=255)
                                                                                                                        aGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATaaa                 >  1:2580016/1‑140 (MQ=255)
                                                                                                                        aGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACAGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGAACAGGAATaaa                 >  2:4970960/1‑140 (MQ=255)
                                                                                                                        aGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAGCCCTACTGTATTTTTCAGCAGTACGGAAGCGATGATCACAGTATCTGTCGCGTCTCTAAACATACGGCCAAGTACAGGAATaaa                 >  1:1917350/1‑140 (MQ=255)
                                                                                                                          cACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                     >  1:4175131/1‑70 (MQ=255)
                                                                                                                          cACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                     <  2:4175131/70‑1 (MQ=255)
                                                                                                                               tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTCCTGTATTTTTCAGCAGTAAGGAAGAGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCg          >  2:4483718/1‑140 (MQ=255)
                                                                                                                               tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCg          >  1:5470364/1‑140 (MQ=255)
                                                                                                                               tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCg          >  1:4100882/1‑140 (MQ=255)
                                                                                                                               tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCg          >  2:5707342/1‑140 (MQ=255)
                                                                                                                               tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGAGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCg          >  2:3152886/1‑140 (MQ=255)
                                                                                                                               tGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTGTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCg          >  1:1901033/1‑140 (MQ=255)
                                                                                                                                    gCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                    >  1:4853692/1‑77 (MQ=255)
                                                                                                                                     ccGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTCCTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTtata    >  1:2505276/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GCGAGAGCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAA  >  NZ_CP034943/2408582‑2408856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: