Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,254 G→T 9.3% Q270K (CAA→AAA)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2540GT 9.3% 472.9 / 5.0 214Q270K (CAA→AAA) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base G (89/96);  new base T (26/3);  total (115/99)
Fisher's exact test for biased strand distribution p-value = 1.53e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGA  >  NZ_CP034943/2502117‑2502393
                                                                                                                                         |                                                                                                                                           
tgTCTGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATAAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTATTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGaa                                                                                                                                           <  2:3536596/139‑1 (MQ=255)
ggTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGaa                                                                                                                                           <  2:3530182/140‑1 (MQ=255)
  tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                                         <  2:4750297/140‑1 (MQ=255)
  tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                                         <  2:266675/140‑1 (MQ=255)
  tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                                         >  2:1854033/1‑140 (MQ=255)
  tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                                         <  2:3804182/140‑1 (MQ=255)
    ggACCGATGATCTCAATTGAGCCGATCTGCTTCTGATCAACTGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAg                                                                                                                                       <  1:3392092/140‑1 (MQ=255)
      aCCGATGATCGCATTTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCt                                                                                                                                     <  2:2996628/140‑1 (MQ=255)
      aCCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCt                                                                                                                                     <  2:745941/140‑1 (MQ=255)
      aCCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTGTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCt                                                                                                                                     <  2:2569218/140‑1 (MQ=255)
         gatgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCAACTCTTCATAGTCGTTTTCTTTCCCCATTTTAAACCAAAACCCCGGCTGTGGGGATAGAACCAGCCTTa                                                                                                                                  >  2:4694945/1‑140 (MQ=255)
          atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGGTTTTAATCGAAATACCCGTGTGTGGGGATTTAACGAGCTTTaa                                                                                                                                 >  1:5291852/1‑140 (MQ=255)
          atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGGTTTTAATCGAAATCCCCGGGTGGGGGGCTTGAACGAGCTTTaa                                                                                                                                 >  2:4893294/1‑140 (MQ=255)
           tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                                                                <  1:3503639/140‑1 (MQ=255)
           tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGAGTTTAATCGAAAAACCCGGGGGTGGGGATTTTACGAGCTTTaaa                                                                                                                                >  1:2501164/1‑140 (MQ=255)
           cgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTGTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                                                                <  1:5710491/139‑1 (MQ=255)
               cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCCTGTGTGGGGATTGAACGAGCTTTAAAAAAt                                                                                                                            >  2:5304517/1‑140 (MQ=255)
                gCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCGTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCATTAAAACATc                                                                                                                           >  2:2793770/1‑140 (MQ=255)
                gCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAAAGATCTTTAAAACATc                                                                                                                           >  2:1444397/1‑140 (MQ=255)
                    ttGAGCCGATCTGCTTCTGATCAACGGAATATGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAATACCCGTGTGTGGGGTTTGAACGCGCGTTAAAACATCCTGt                                                                                                                       >  1:1602537/1‑140 (MQ=255)
                         ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTAACGAATTTAATAGAAAAACCCGGGTTTGGGGATTTAAAGAGCAATAAAAAATCCAGTTAttt                                                                                                                  >  1:4999411/1‑140 (MQ=255)
                         ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                                  <  1:3443584/140‑1 (MQ=255)
                         ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                                  <  1:1854033/140‑1 (MQ=255)
                         ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTCCCCATTTTTATCGAAATCCCCGTGTGTGGGGATTGAAAGAGCTTTAAAAAATACTGTTCttt                                                                                                                  >  1:1697959/1‑140 (MQ=255)
                            aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttta                                                                                                               >  2:5371698/1‑139 (MQ=255)
                            aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCGTTAAAAGATACTGTTCttttta                                                                                                               >  2:3294992/1‑139 (MQ=255)
                            aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGCTTGAACGAGCTTTATAACATGCTGTTCtttttg                                                                                                               >  2:3696421/1‑139 (MQ=255)
                            aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGGGGGGATTGAACGAGCGTTAAAACATCCGGTTCtttttg                                                                                                               >  1:3552098/1‑139 (MQ=255)
                                 cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGGGCTTTTAATCAATCTGTTCTTTTTCAAttg                                                                                                          >  2:3074094/1‑139 (MQ=255)
                                      gATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCGTCATAGTCGTTTTCTGTCCCCATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATtag                                                                                                     >  2:1741613/1‑140 (MQ=255)
                                       aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAATCCCCGGGTGGGGGGCTTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgaga                                                                                                    >  2:2278340/1‑140 (MQ=255)
                                       aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGTGCTGTAAAACCTCCTGTTCTTTTTAAATTAATtaga                                                                                                    >  2:5224949/1‑140 (MQ=255)
                                           aCGGAATACGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                                <  2:4494469/140‑1 (MQ=255)
                                           aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGGGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                                >  2:5515764/1‑140 (MQ=255)
                                           aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGAGTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGATAGAAg                                                                                                >  2:183869/1‑140 (MQ=255)
                                           aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAg                                                                                                >  2:4663956/1‑140 (MQ=255)
                                            cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGCTTTTAATAGAAATCCCCGGGGGTGGGGATTGACCGAGCGTTAAAAACTGCTGTTGTTTTTCGATTAATGGGAGAAGc                                                                                               >  1:3376743/1‑140 (MQ=255)
                                                aTACGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAAGGAGAGAAGCGAcc                                                                                           <  1:239104/140‑1 (MQ=255)
                                                aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                                           <  1:4886822/140‑1 (MQ=255)
                                                aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                                           <  1:1103281/140‑1 (MQ=255)
                                                   agaagCCTTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGCGAGAAGCGACCGaa                                                                                        <  1:2116933/140‑1 (MQ=255)
                                                   agaagCCGTAATCAGACTGCATTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGATTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                                                                        <  1:1817804/140‑1 (MQ=255)
                                                   agaagCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                                                                        <  2:5314805/140‑1 (MQ=255)
                                                   agaagCCGTAATCAGACTGCAGTTTTCCAACTCTTCATCGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGGGGGGATTGAACGAGCTTTAAAACATCGTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                                                                        <  1:845866/140‑1 (MQ=255)
                                                     aagCCTTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                                                                      <  2:2014206/140‑1 (MQ=255)
                                                     aagCCGTAATCAGACTGCCGTTTGCCAGCTCTTCATCGTCGTTGTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                                                                      <  1:3659850/140‑1 (MQ=255)
                                                     aagCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                                                                      <  2:1128576/140‑1 (MQ=255)
                                                      agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATGCGGTTCTTTTTCAATTAATGAGAGAAGCGACCGAActc                                                                                     >  2:3202813/1‑140 (MQ=255)
                                                      agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAActc                                                                                     <  2:1641575/140‑1 (MQ=255)
                                                      agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAActc                                                                                     <  2:2123686/140‑1 (MQ=255)
                                                       gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTAAATTAATTAGAGAAGCGACCGAActct                                                                                    >  2:1868344/1‑140 (MQ=255)
                                                        ccGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCGGTTCTTTTTCAATTAATGAGAGAAGCGACCGAActcgg                                                                                   >  2:3994087/1‑138 (MQ=255)
                                                         cGTAATCAGACTGCTGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                                  <  1:548428/140‑1 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTATTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                                  >  2:5095464/1‑140 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAAACCCCGGGTGTGGGGATTTGACGAGCGTTAAAACATCCTGTTCTTTTTAAATTAATGATAGAAGCGAACGAActaggg                                                                                  >  1:3213015/1‑136 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGAGTTTAATCGAAATCCCCGGGGGTGGGGGTTTAAAGAGCTTTAAAAAATCCTGTTCTTTTTAAATTAATTAGAGAAGCGACCCAActaggg                                                                                  >  1:632974/1‑136 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGCTTGAACGTGCTTTAAAACAGACTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                                  >  2:1030577/1‑140 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                                  >  2:2235848/1‑140 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACCTCCTGTTCTTTTTCAATTAATGAGAGAATCGACCGAACTCggg                                                                                  >  2:4850420/1‑140 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGTTTTAACGAGCTTTAAAACATCCTGTTGTTTTTGAATTAATGAGAGAAGCGACCCaaagcggg                                                                                  >  1:2288409/1‑134 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATAGAAATACCCGTGTGTGGGGCTTGAACGAGCTTTAAAACATCCTGTTATTTTTGAATTAATGATAGAAGCGACCGACCGCTgg                                                                                  >  1:1679066/1‑140 (MQ=255)
                                                         cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCGTTCCCCATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCGTTAAAACCTCCGGTTCTTTGTCAATTAAGGGGAGAAGCGACCGAACTCTgg                                                                                  >  2:4344673/1‑140 (MQ=255)
                                                         cGTAATCAGACTGCAGTGTTCCATCTCTTCATAGTCGTGTTGGTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGTTTGTACGAGCGTTAAAACATGCTGTTATTTTTCAATTAATTAGAGAAGCGACCGAACTCggg                                                                                  >  2:2002805/1‑140 (MQ=255)
                                                              tCAGAGTGCTTTTTTCCATCTCTTAATAGTCTTTTTCTTTCCCGATTTTATTCGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTATTGAGAGAAGCGACCGAACTCTGGTGata                                                                             <  1:90503/140‑1 (MQ=255)
                                                              tCAGAGTGCAGTTTTCCATCTCTTCATAGTCTTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCACTTAATGAGAGAAGCGACCGAACTCTGGTGata                                                                             <  1:69275/140‑1 (MQ=255)
                                                              tCAGACTGCAGTTTTCCATCTCTTCATAGTCTTTTTCTTTCCCGATTTTAATATAAATCCCCGTGTGTGGGTATTGAACGAGATTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                                                                             <  2:4601684/140‑1 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCAt                                                                         <  2:106064/140‑1 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAAGAGGTTTAAAAGATCCTGTTGTTTTTGAATTAATGAGTGAATCGGCCGAACACGGGTGATATCAt                                                                         >  1:5628527/1‑140 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGGGGGTGGGGCGTGAACGAGCTTTAAAACATACTGTTCTTTTTGAATTAATGGTATAAGCGACCGAAATATGGGGATATCAt                                                                         >  1:3953066/1‑140 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAATCCCCGGGTGTGGGGATTGTACGAGCGTTAAAAAATCCGGTTATTTTTAAATTAAAGAGAGAAGCGACCGGAAGCTGGTGATATAAt                                                                         >  1:3448303/1‑140 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGTAATACCCGGGGGGGGGGTTTGTACGAGCGTTAAAAAATACTGTTGTTTTTGAATTAATTTGTGTATCGTCCGAACTCGGGTGGTATCAt                                                                         >  1:1905075/1‑140 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATACCCCGGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTATTTTTTAAATAATGATGGTAGCGACCGAACTCTGGTGttatgaa                                                                         >  1:3669992/1‑137 (MQ=255)
                                                                  aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTACCGATTTTAAAAGAAAAACCCGGGTGTGGGGATTTAAACAGCTTTAAAAAATACTGTTGTTTTTAAATTAATTATATAAGCGACCGAACGATGGTGATATCAt                                                                         >  1:3007571/1‑140 (MQ=255)
                                                                   cTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATg                                                                        <  1:649519/140‑1 (MQ=255)
                                                                        gTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACt                                                                   <  1:2837841/140‑1 (MQ=255)
                                                                             ccctctcTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                              <  1:4407747/137‑1 (MQ=255)
                                                                             cccgctctTCATCGTCCTGTTCTTTCCCGATTGTACTCGAAAGCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                              <  2:3336983/136‑1 (MQ=255)
                                                                             ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                              <  2:4447688/140‑1 (MQ=255)
                                                                             ccATCACTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                              <  2:4197975/140‑1 (MQ=255)
                                                                                tctcTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATTATTAAAGCGAACGAACGCGGGTGATATACTGGAAAACCGGCTgg                                                           >  2:1362351/1‑140 (MQ=255)
                                                                                  tctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                                         <  2:2613854/140‑1 (MQ=255)
                                                                                   ctTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAAACCCCGTGTGTGGGGATTTAACGAGCTTTAAAAAATCCTGTTATTTTTAAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTc                                                        >  2:876530/1‑140 (MQ=255)
                                                                                   ctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTATTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGGGATATCATGGAACTCCGGCTGGTTc                                                        >  2:600687/1‑140 (MQ=255)
                                                                                     tCATAGTCGTGTTGTTTCCCGATTTTAATAGAAATACCCGGGTGTGGGGATTGAACGAGCGTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAATCGACCGAAATCTGGTGATATCATGGAAATACGGCTGGTTCAg                                                      >  2:173714/1‑140 (MQ=255)
                                                                                      cATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCCTGTGTGGGGATTGAACGAGCTTTAAAACAACCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtggtgc                                                     >  2:5713165/1‑135 (MQ=255)
                                                                                           tCGTTTTCTTTCCCGATTTTAATAGAAAAACCCGTGGGTGGGGATTGAACGAGCTTTAAAACAACCTGTTCTTTTTCAATTAAAGAGAGAAGCGACCGAACTCTGGTGATATCATGGAAATCCGGCTGGTTCAGCATAtt                                                >  2:1097210/1‑140 (MQ=255)
                                                                                              ttttCTTTACCGATTTTAATCGAAATACCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGTGAGAAGACACCGAACTCTGGTGATATAATGGAACTCCGGCTGGTTCAGCATATTGAt                                             >  2:3476770/1‑140 (MQ=255)
                                                                                                     tcCCGATTTTAATCGAAAACCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATGCAGTTCTTTTTAAATTAATGAGAGAAGCGACCGAAATCTTGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGAtttttgag                                      >  1:4139518/1‑137 (MQ=255)
                                                                                                           ttttAATCGAAAGCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGaaa                                <  2:3912011/140‑1 (MQ=255)
                                                                                                             ttAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGaaaaa                              <  2:3631918/140‑1 (MQ=255)
                                                                                                                     aaTCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAAttttt                      >  2:1292961/1‑140 (MQ=255)
                                                                                                                     aaTCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAAttttt                      >  2:5457885/1‑140 (MQ=255)
                                                                                                                     aaTCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAAttttt                      >  2:2516909/1‑140 (MQ=255)
                                                                                                                             tgtgtgGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGa              >  2:4947438/1‑140 (MQ=255)
                                                                                                                             tgtgtgGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGa              >  1:2677531/1‑140 (MQ=255)
                                                                                                                                   gggATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCAGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTtgtg        >  2:666713/1‑140 (MQ=255)
                                                                                                                                     gATTGCACGAGCATTAAAACATCCTGTTCATTTACAATTCAGGACAGAAGCGACCGAACTCTGGTGATATCAGGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGa      <  1:756682/140‑1 (MQ=255)
                                                                                                                                        tGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATg   >  2:1556430/1‑140 (MQ=255)
                                                                                                                                         gAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGa  >  2:3420147/1‑140 (MQ=255)
                                                                                                                                         gAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGa  >  1:1523086/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGA  >  NZ_CP034943/2502117‑2502393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: