Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,709,410 T→C 5.1% L22L (TTA→CTA)  EO946_RS13995 → YsnF/AvaK domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,709,4100TC 5.1% 722.2 / 15.2 214L22L (TTA→CTA) EO946_RS13995YsnF/AvaK domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (117/84);  new base C (5/6);  total (124/90)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

TGTTTATTTTAAAGAGCATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATA  >  NZ_CP034943/2709271‑2709548
                                                                                                                                           |                                                                                                                                          
tGTTTATTTTAAAGAGCATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCttat                                                                                                                                            <  2:3246698/140‑1 (MQ=255)
tGTTTATTTTAAAGAGCATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCttat                                                                                                                                            >  1:3246698/1‑140 (MQ=255)
      ttttAAAGAGCATCGGGAATTACACACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACaa                                                                                                                                      <  1:1245188/140‑1 (MQ=255)
      ttttAAAGAGCATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACaa                                                                                                                                      <  1:2672719/140‑1 (MQ=255)
      ttttAAAGAGCATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACaa                                                                                                                                      <  1:42192/140‑1 (MQ=255)
                cATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGa                                                                                                                            >  1:2198427/1‑140 (MQ=255)
                  tCGGTAATTACAAACGACATTAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGAtt                                                                                                                          <  2:2890361/140‑1 (MQ=255)
                  tCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGAtt                                                                                                                          <  2:2178764/140‑1 (MQ=255)
                  tCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGAtt                                                                                                                          <  1:2279189/140‑1 (MQ=255)
                  tCGGCAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGAtt                                                                                                                          <  2:4991223/140‑1 (MQ=255)
                     ggAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTg                                                                                                                       >  2:583872/1‑140 (MQ=255)
                     ggAATTACAAACGACAAGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATAGGAAAAGTAAAGTTGGTGTCTACGAACCTCCGCAAGAAAACAGAGCCGCGATTGAAGGGTTAGTAACACAAGGCTATGATGCTg                                                                                                                       >  2:3752087/1‑140 (MQ=255)
                       atttACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTACTAACAAAAGGCTATGATTCTgac                                                                                                                     <  2:1293020/138‑1 (MQ=255)
                         ttACAAACGACATGAACTATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTgacga                                                                                                                   <  1:3077219/140‑1 (MQ=255)
                         ttACAAACGACATGAACGATAAAATGAATATTGATAAATGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTgacga                                                                                                                   <  2:1171567/140‑1 (MQ=255)
                          tACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTgacgac                                                                                                                  >  1:3429644/1‑140 (MQ=255)
                          tACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTgacgac                                                                                                                  >  2:2673099/1‑140 (MQ=255)
                                    aTGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCtgttg                                                                                                        >  1:3319056/1‑140 (MQ=255)
                                    aTGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCtgttg                                                                                                        >  1:5671870/1‑140 (MQ=255)
                                           aTAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATTATTCTGACGACATCTCTGTTGTGACaat                                                                                                 >  1:5730553/1‑139 (MQ=255)
                                           aTAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTgac                                                                                                                     <  2:162218/120‑1 (MQ=255)
                                           aTAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAg                                                                                                 >  2:4416697/1‑140 (MQ=255)
                                               aaTGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCgg                                                                                             <  2:1409117/140‑1 (MQ=255)
                                                aTGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGc                                                                                            <  2:1477600/140‑1 (MQ=255)
                                                 tGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGAtt                                                                                                                          >  2:2276719/1‑109 (MQ=255)
                                                    aaaTTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCgaga                                                                                        >  2:2519010/1‑140 (MQ=255)
                                                      aTTCATAAAGGAGGAGATTGATATGAAAAGCATAGTTGGTGTATATGAAACTCCGCAGGAAACCATCGCAGCGATTGAAGGCTTACTTACAAAAGGCTATGATTCTGATGACATTTCTGTTGTGACAAGCCGGCGAGATa                                                                                      >  1:448918/1‑140 (MQ=255)
                                                      aTTCATAAAGGAGGAGATTGATATGAAAAGCATAGTTGGTGTATATGAAACTCCGCAGGAAACCATCGCAGCGATTGAAGGCTTACTTACAAAAGGCTATGATTCTGATGACATTTCTGTTGTGACAAGCCGGCGAGATa                                                                                      >  1:4775672/1‑140 (MQ=255)
                                                      aTTCATAAAGGAGGAGATTGATATGAAAAGCATAGTTGGTGTATATGAAACTCCGCAGGAAACCATCGCAGCGATTGAAGGCTTACTTACAAAAGGCTATGATTCTGATGACATTTCTGTTGTGACAAGCCGGCGAGATa                                                                                      >  1:987396/1‑140 (MQ=255)
                                                                  ggagATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTg                                                                          >  1:1502375/1‑140 (MQ=255)
                                                                       ttAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAaca                                                                     <  1:3749611/140‑1 (MQ=255)
                                                                       ttAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAaca                                                                     <  1:1909044/140‑1 (MQ=255)
                                                                          atatGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGc                                                                  >  1:2878638/1‑140 (MQ=255)
                                                                          atatGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGc                                                                  >  1:5266508/1‑140 (MQ=255)
                                                                           tatGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGca                                                                 >  1:2150757/1‑140 (MQ=255)
                                                                            atGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                <  1:3531849/140‑1 (MQ=255)
                                                                            atGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                <  1:115855/140‑1 (MQ=255)
                                                                            atGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                <  1:4619380/140‑1 (MQ=255)
                                                                            atGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                <  1:536746/140‑1 (MQ=255)
                                                                              gAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAg                                                              <  2:2017269/140‑1 (MQ=255)
                                                                                aaaGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGTCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGa                                                            >  2:810151/1‑140 (MQ=255)
                                                                                aaaGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGa                                                            >  2:1528166/1‑140 (MQ=255)
                                                                                aaaGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGa                                                            >  2:1353263/1‑140 (MQ=255)
                                                                                aaaGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGa                                                            >  1:2271675/1‑140 (MQ=255)
                                                                                aaaGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGa                                                            >  2:224251/1‑140 (MQ=255)
                                                                                 aaGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGATTATTAACAAAAGTCTATGATTCTGACGACAACTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGaa                                                           <  2:456160/140‑1 (MQ=255)
                                                                                  aGTATCGTTGTTGTATACGAAACGCCGCAACCAACCATTGGAGCGATTGAAGGCTTATTACCAAAAGGATATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAAc                                                          <  2:2935633/140‑1 (MQ=255)
                                                                                      taGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGaa                                                      <  2:5357417/140‑1 (MQ=255)
                                                                                      taGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGaa                                                      <  2:5671870/140‑1 (MQ=255)
                                                                                           ggTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCaa                                                 >  1:7100/1‑140 (MQ=255)
                                                                                            gtgtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAAt                                                <  1:1003462/140‑1 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTCTGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGACCAGAAGTCAATcc                                              >  1:679961/1‑139 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAAt                                                >  2:714051/1‑138 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:2694017/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:2570847/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:2490130/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:312482/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  2:5231032/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:1898393/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:1842727/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:5574454/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  2:5717816/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCa                                              >  1:1641569/1‑140 (MQ=255)
                                                                                              gtATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTAATAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAACCACGCCCAGGAACTGAAGTCAATCa                                              >  1:5142941/1‑140 (MQ=255)
                                                                                                 taCGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGACCAGAAGTCAATCAGGc                                           >  1:3980173/1‑140 (MQ=255)
                                                                                                 taCGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGc                                           >  1:3798438/1‑140 (MQ=255)
                                                                                                 taCGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGc                                           >  1:2838338/1‑140 (MQ=255)
                                                                                                    gAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCAt                                        <  2:417320/140‑1 (MQ=255)
                                                                                                    gAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCAt                                        <  2:87716/140‑1 (MQ=255)
                                                                                                      aaCTCCGCAAGAAACCATTGCCGCCATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTCTCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTg                                      >  1:4015975/1‑140 (MQ=255)
                                                                                                      aaCTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACCAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGACCAGAAGTCAATCAGGCCATTg                                      >  2:5100421/1‑140 (MQ=255)
                                                                                                      aaCTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGa                                                                                  >  1:355673/1‑96 (MQ=255)
                                                                                                       aCTCCGCAAGAAACCATTGCAGCGATTGAAGGCGTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAATCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGa                                     >  1:2800508/1‑140 (MQ=255)
                                                                                                        cTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGAt                                    <  2:4084124/140‑1 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                <  1:5686987/111‑1 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                >  2:2818715/1‑111 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                >  2:4724416/1‑111 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATg                                   >  2:5172834/1‑140 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATg                                   >  1:807299/1‑140 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATg                                   >  2:3605384/1‑140 (MQ=255)
                                                                                                         tCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATg                                   >  2:1166498/1‑140 (MQ=255)
                                                                                                           cGCAGGAAACCATCGCAGCGATTGAAGGCTTACTTACAAAAGGCTATGATTCTGATGACATTTCTGTTGTGACAAGCCGGCGAGATACAGATTATTTGGAGTCACGTACAGGAACAGAAGTCAATCAAGCTATCGATg                                   <  1:4116206/138‑1 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACg                                                                   >  2:3509182/1‑106 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGcac                                                                >  2:5511654/1‑109 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGaa                                                      >  1:4911963/1‑119 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGaa                                                      <  2:4911963/119‑1 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGc                                           <  2:3425805/130‑1 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCAt                                        >  2:4632396/1‑133 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCAt                                        >  2:1739878/1‑133 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCa                                 >  1:886089/1‑140 (MQ=255)
                                                                                                           cGCAAGAAACCATTGCAGCGATTGAAGGCTGATTAACAAAAGGCTAAGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGACATTGATGCa                                 >  1:5414058/1‑140 (MQ=255)
                                                                                                             cAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACg                               >  1:4074755/1‑140 (MQ=255)
                                                                                                                               aTTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATGGATGCACGTGAGGGCgaatctgaatc             >  1:215921/1‑140 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATg                                   <  1:1701667/107‑1 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGACTCTGAATCTTTTTTCGATa  >  2:4282970/1‑140 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATa  >  2:2126241/1‑140 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATa  >  1:3840880/1‑140 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATa  >  1:5069332/1‑140 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATa  >  2:3584474/1‑140 (MQ=255)
                                                                                                                                          attaACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATa  >  2:3894619/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGTTTATTTTAAAGAGCATCGGGAATTACAAACGACATGAACGATAAAATGAAAATTGATAAAGGAGGAGATTAATATGAAAAGTATAGTTGGTGTATACGAAACTCCGCAAGAAACCATTGCAGCGATTGAAGGCTTATTAACAAAAGGCTATGATTCTGACGACATCTCTGTTGTGACAAGCCGGCGAGATACAGATTATCTTGAAACACGCACAGGAACAGAAGTCAATCAGGCCATTGATGCACGTGAGGGCGAATCTGAATCTTTTTTCGATA  >  NZ_CP034943/2709271‑2709548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: