Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,591 A→T 8.7% intergenic (+269/‑35) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5910AT 8.7% 1657.2 / 21.3 586intergenic (+269/‑35)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (136/379);  new base T (48/2);  total (202/384)
Fisher's exact test for biased strand distribution p-value = 2.56e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 587 total aligned reads displayed.

TCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGC  >  NZ_CP034943/112455‑112729
                                                                                                                                         |                                                                                                                                          
  aGTATTCAATGGCGGCCGCCGGCGTTACAGTCTGAAGTTTGGAGAGCATAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGTgg                                                                                                                                       <  2:4948421/140‑1 (MQ=255)
  aGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAgg                                                                                                                                       <  2:545909/140‑1 (MQ=255)
     aTTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAAGCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGaaa                                                                                                                                    <  2:4515540/140‑1 (MQ=255)
            ggcggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATa                                                                                                                             <  2:5714063/140‑1 (MQ=255)
              cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCACACCGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCTTGCAGAGGCGCTTTTTTTATTGGGTGTCGAGGAACTCAAAgaga                                                                                                                           >  1:2207578/1‑140 (MQ=255)
               ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                          <  2:2039008/140‑1 (MQ=255)
               ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                          <  2:2753206/140‑1 (MQ=255)
                gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCCGAGGCGCTTTTTTTATTGGGTGAGGAGGCAATCCAATAgaga                                                                                                                         >  2:4747076/1‑140 (MQ=255)
                gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGGAATCAAATagagt                                                                                                                         >  1:4177336/1‑139 (MQ=255)
                gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAgaga                                                                                                                         >  1:3324071/1‑140 (MQ=255)
                gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAAAAgaga                                                                                                                         >  2:1963863/1‑140 (MQ=255)
                 gcgccgccgTTACCGGCTGAAGTTTGGAGCTCAGATGTCTGGTTTTCAACCAGCGGGGAACCGCGCGGTTTAATCGTCTCTGTCATGTTTTCATGGAGATGCTGTTTTTTTATTGGGTTAAGAGGAAAACAAATAcagat                                                                                                                        <  2:3167880/139‑2 (MQ=255)
                 ccgccgccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGTAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTTAAGAGGAAATCAAATAGAGaa                                                                                                                        <  2:5064364/140‑1 (MQ=255)
                          ttACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGa                                                                                                               <  1:4671126/140‑1 (MQ=255)
                             cAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                                                                            <  1:1858094/140‑1 (MQ=255)
                             cAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                                                                            <  1:1246066/140‑1 (MQ=255)
                                  tGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTTAAGAGGAAAAAAAATACAGTAGTGGGGGGAAGCAagtt                                                                                                       >  1:5598980/1‑139 (MQ=255)
                                      gTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGtttt                                                                                                   <  1:802117/140‑1 (MQ=255)
                                           gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                              <  2:2555324/140‑1 (MQ=255)
                                           gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                              <  1:3866702/140‑1 (MQ=255)
                                           gagaGCAGAAGTCTGCTTTTAAAACAGAGTGGAACCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAGGTGTTTTTTAGa                                                                                              <  1:5209335/140‑1 (MQ=255)
                                                 aGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTc                                                                                        <  1:4418506/140‑1 (MQ=255)
                                                 aGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTCTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTc                                                                                        <  1:4069686/140‑1 (MQ=255)
                                                     gTCTGCTTTTCACACTGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                    <  2:3752890/140‑1 (MQ=255)
                                                       cTGGTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGTCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                  <  2:34804/140‑1 (MQ=255)
                                                       cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                  <  1:3693643/140‑1 (MQ=255)
                                                       cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                  <  1:1519462/140‑1 (MQ=255)
                                                       cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                  <  1:1666306/140‑1 (MQ=255)
                                                       cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                  <  2:3329585/140‑1 (MQ=255)
                                                       cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTGATTGGGTGAAGAGGAAAGCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                  <  1:3055811/140‑1 (MQ=255)
                                                        tGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGTAGAGGTAATCAAATATAGAAGTGGGGGGCAGCATGTGTTTTTTATAATTCTCAaataaa                                                                                 >  1:4516518/1‑139 (MQ=255)
                                                        tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGTAGAGGAAATCAAATATAGAAGTGGGGGGAACCCGGTGTTTTTTTGTATTGTCAaataaa                                                                                 >  1:1888194/1‑139 (MQ=255)
                                                        tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTGTTTTTTTTGGTGTATGGGGAATCAAAAATAGAAGTGGGGGGAAGCATTTGGTTTTTTGAATTCTCAaagaac                                                                                 >  1:473292/1‑139 (MQ=255)
                                                         gCTTTTCTAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATTCAGAGTCGCTTTTTTTATTGGGTGAATAGGAAATCAAATAGAGAAGGGGGGGGAAGAATGTGTTTTTTAGAATGCTCAAagaaga                                                                                <  2:1401101/140‑1 (MQ=255)
                                                         gCTTTTCAAGCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                <  1:2659850/140‑1 (MQ=255)
                                                         gCTTTTCAAACAGAGTGGAACCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                <  2:2363702/140‑1 (MQ=255)
                                                         gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTGATGTTTTCATGCAGAGGAGCTTTTTTTATTGGGTGAAGAGCAAATCACATAGAGAAGTGGGGCGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                <  1:4824053/140‑1 (MQ=255)
                                                         gCTTTTCAAACAGAGTGGAACCGCGCGGATAAAGCGTCTCTGTCATGTTTTCCTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagcaga                                                                                <  1:2509285/140‑1 (MQ=255)
                                                                 aaCTGAGTGGAACCGCGCGGTTTAAGAGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                        <  1:5479823/140‑1 (MQ=255)
                                                                 aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGACATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                        <  1:1077712/140‑1 (MQ=255)
                                                                 aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                        <  2:2679267/140‑1 (MQ=255)
                                                                 aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                        <  2:1222995/140‑1 (MQ=255)
                                                                 aaCAGAGTGGAACCGCGCGGTTTAAGCGTATCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                        <  1:5082916/140‑1 (MQ=255)
                                                                  aCAGTGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTATTGGGTGAAGAGGAAAACACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                        <  1:1015190/140‑1 (MQ=255)
                                                                      agTGGAACCGCGCGGTTTAAGCGTCTCTGGCAAGATTTCATGCCCAGGCGCTGTTTGTAGTGTGTGAAAAAGACAACCAATATAGTAGAGGGGGGAAGCCTGTGTTATTTAGAAATATCAACTAAGACATAGATACAgtg                                                                   >  2:5156386/1‑140 (MQ=255)
                                                                            aCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAt                                                             <  2:4043411/140‑1 (MQ=255)
                                                                              cgcgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCa                                                           <  2:4352658/140‑1 (MQ=255)
                                                                                cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGTGGAAATAAAATAGATAAGTGGGGGGGAGCATGTGTTTTTTTTAATTCTAAAAATAAAAAATTATAAATTGTTCGAACaat                                                         >  1:2832304/1‑139 (MQ=255)
                                                                                cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGGAATGAAATAGAGAACTGGGGGGGAGCATGTGTTTTTTTTAAAGCTCAAAAAACACATTTTTACATTTTTCTATaaat                                                         >  1:400952/1‑139 (MQ=255)
                                                                                cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCCAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTTGAATGCTCAAAGAAGACATTGATACCTTGTTCGATCaat                                                         >  2:4225705/1‑139 (MQ=255)
                                                                                cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTGTTTTTATTGGGTGAAGATGAAATCAAATATAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTAAAAGAAGACATTGATAAATTGTTCGATCaat                                                         >  2:1089671/1‑139 (MQ=255)
                                                                                  cgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGAt                                                       <  1:5378792/140‑1 (MQ=255)
                                                                                             tctcTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGTGGAGAGGTAAACAAAAAAAGAACTGGGGGGGAACAAGTGTTTTTTTTAAATATCAAAGAAAAAAATTATAAACTTTTAGATCAAAAAACAGCACCaaca                                           >  1:970179/1‑138 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACACTTTTAGATCAAGATACAGCAGCaagaa                                          >  2:2198324/1‑140 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGGAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAAGACATTTATAAAGTGTTCGATCAAGATCCAGCAGCaagaa                                          >  2:3504206/1‑140 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGTAAGATGAAAACAAATAAATAAGTGGGGGGACGCAGGTTTTTTTTATTATGGTAAAAAAAGACATTTATAAACGTTTAGATAAAAATACAGCACCaaaaa                                          >  1:1780340/1‑140 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGTAATCAAATAGAGAAGGGGGGGGCAGCAGGTGTTTTTTAGAATTCTCCAAGTAGACATTGGTTCAGTGTTGGGGCAAGTTCCAGCAGCcagac                                          >  2:5542908/1‑139 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                          >  2:2838722/1‑140 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATAGAGAAGTGTGGGGAATCATGTGTTTTTTTGTATGCGCAAATAAGACATTGATCCATTGGTCGTTAAAGATCCAGCCGCaagaa                                          >  1:2735574/1‑140 (MQ=255)
                                                                                               tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTTGGGGTATAGGTAATCAAATAAATAAGTGGGGGGGAGCAAGTGTTTTTTTTAAATGTCAAATTAAACCTTTATACAGTGTTAGATCAAAATCCAGCAGCaaaaa                                          >  1:647239/1‑140 (MQ=255)
                                                                                                ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                         <  1:1401771/140‑1 (MQ=255)
                                                                                                ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                         <  1:2848451/140‑1 (MQ=255)
                                                                                                ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACCAATAGAGAAGTGGGGGGAAGCATGTCTTTTTTAGACTTCTCAAAGAAGACATTGATACAGTGTTGGATCAAGATCCAGCAGCaagaag                                         <  1:3195905/140‑1 (MQ=255)
                                                                                                        ttttCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAAAAAAAAATAGTAGTGGGGGGGAGCATGTGTTTTTTTGAATTCTCAAAGAAAAAAATTATAAAGTGTTAGATAAAGATACACCCGCAAGAAGTTAttttt                                 >  1:2988601/1‑139 (MQ=255)
                                                                                                        ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                >  2:341610/1‑93 (MQ=255)
                                                                                                        ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAGTGTTCGATAAAGATCCAGCAGCAAGAAGGTAttttt                                 >  2:3975031/1‑139 (MQ=255)
                                                                                                          ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                               <  1:3940279/140‑1 (MQ=255)
                                                                                                          ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                               <  2:483153/140‑1 (MQ=255)
                                                                                                          ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                               <  1:3369391/140‑1 (MQ=255)
                                                                                                                cAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAtt                         <  1:359824/140‑1 (MQ=255)
                                                                                                                cAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAtt                         <  1:4555497/140‑1 (MQ=255)
                                                                                                                  gagGCGCTTTTTTTATTGGGTGAATAGGAAATAAAAAAGAGGAGTGGGGGGGAGCATGTGTTTTTTAGTATTCTAAAAAAAGACCTTGATACAGTGTTAGATCAAGATCCACCAGCAAGAAATTATTTTGTAGTGAtttt                       >  2:83963/1‑140 (MQ=255)
                                                                                                                   agGCGCTTTTTTTATTGGGGGTAGAGGGAATAAAATAGAGAAGTGGGGGGCAGCATTTGTTTTTTATAAAGCGCAAAGAAGAAAATGATAAAATGTTAGATAAATATACAGCACCAAAAAGTTAGTTTTAAGTGATTTTa                      >  2:738481/1‑140 (MQ=255)
                                                                                                                        cTTTTTTTATTGGGTGAAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAAAGCTCAAAGAAGAAAATTATAAAGTGTTAGATAAAGATACATCAGCAAGAAGTTATTTTTAAGTTATTTTAAATTa                 >  2:5399411/1‑140 (MQ=255)
                                                                                                                        cTTTGTTTATTGGGTGAAGAGGCACTCAAATAGGGAAATGGGGGGAAGCATGTGTTTTTTAGAATGGTCAAACAAGACAATGAAACACAGTTCCGTCCACATCCCGCCGCAAGAACTTATTTTTGAGTGATTTTAACTTa                 >  2:458266/1‑140 (MQ=255)
                                                                                                                           tattttATTGGCTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTAtt               <  2:905039/138‑1 (MQ=255)
                                                                                                                            tttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc              <  1:3455160/140‑1 (MQ=255)
                                                                                                                            tttttATGGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc              <  2:1632617/140‑1 (MQ=255)
                                                                                                                             ttttATTGGGTGCCGAGCAAATCAAATAGAGAAGTGGGGGGAACCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTcc             <  1:4959547/140‑1 (MQ=255)
                                                                                                                             ttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTcc             <  2:3073563/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCACATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  1:2018657/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  2:5640091/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  1:425662/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  1:3543847/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  1:738481/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  2:1176154/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  2:3858967/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  2:3666599/140‑1 (MQ=255)
                                                                                                                                   tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa       <  2:1888194/140‑1 (MQ=255)
                                                                                                                                     ggTGAGGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGACGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTGAACTTATTCCGGTTTACa     <  1:433267/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGTAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  2:150438/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCACATAGAGAAGTTGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  1:582186/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  2:611134/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  1:2141159/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  2:4099019/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  2:1730979/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  2:326925/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  2:2125360/140‑1 (MQ=255)
                                                                                                                                     ggTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa     <  1:3797478/140‑1 (MQ=255)
                                                                                                                                        gAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGc  <  1:5748101/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGC  >  NZ_CP034943/112455‑112729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: