Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,978,155 G→A 16.7% noncoding (2646/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,978,1550GA16.7% 0.8 / 10.8 38noncoding (2646/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (6/25);  new base A (2/4);  total (8/30)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

AGATGCTTTCAGCACTTATCCCGTCCGCACATAGCTACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGT  >  NZ_CP034943/2978016‑2978290
                                                                                                                                           |                                                                                                                                       
aaatccTTTCAGCACAAATCCCGTCCGCACAAAGCTACACAGAGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATACAGGTCCTCACGTACTAAGTACACCTCCTCTCAAATATCCGGCGCCCGCGACg                                                                                                                                         >  2:120340/6‑140 (MQ=1)
   tggtttCAGAACTTATCCCGTCCGCACATAGCAACCCAGCGTGGCCGTTGGAAGAACACGGGGTACACCCGGGGTGCTTCAATCCGGGGCCTATCTAACTACGCACAGCTCCTTAAAATTTTCCTGCGCCGGCGACgttt                                                                                                                                      <  2:275598/137‑4 (MQ=0)
   tgcgtgcAGCACTTCGCCCGTCCGCACATAGCGACCCCGCGAGGCCCGGGGCAGAACAACTGGGACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGAt                                                                                                                                      <  1:3843185/134‑1 (MQ=1)
             actgagccccTCCGCACCGAGCTCCCCAGCGAGGCCCCGGGCAGAACAACGCCTACACCAGCGGTGCGTCCCTCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGaa                                                                                                                            <  2:3453295/134‑1 (MQ=1)
             acgtATCCCGTCCGCACATCGCTACCCAGCGATGCCCTTCGCAGAACAACTGGTACACCAGCGGTGCGTCCAGCCCGGTCCTCTCCTACTAAGCACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGaa                                                                                                                            <  2:4477630/137‑1 (MQ=1)
             acgtATCCCGTCCGCACATAGCTACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGGACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGaa                                                                                                                            <  2:1527800/137‑1 (MQ=1)
              ctgaTCCCGGCCGCACCTAGCGACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAAc                                                                                                                           <  1:2201495/137‑1 (MQ=0)
                             cagaGCTACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTc                                                                                                            <  1:3031604/137‑1 (MQ=1)
                                 gcgaCCCAGCGATGCCCTTGGCAGAACAACTGGTACACCCGCGCTGCGTCCATCCCGGTCCGCTCGTACTAAGGACAGCTCCTCTCAAAATTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGaa                                                                                                        <  1:168528/137‑1 (MQ=1)
                                 gcgaCCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGaa                                                                                                        <  1:4430876/137‑1 (MQ=1)
                                          cgctGCCCGTGGCAGAACACCTGGTACACCAGCGGTGCGTCCAGCCCGGGCGTCTCGTGCTAAGGAGAGCGGCGCGCAACTTGCCTGCGCGCGCGACGGATCGGGCCCGCACTGTCTCACGACGTGCGGACCCCAGCTcg                                                                                               >  2:4186972/4‑140 (MQ=0)
                                              gcgcTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCGCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTGTGAACCCAGCTCGCGTa                                                                                           <  2:3952262/137‑1 (MQ=0)
                                              gcccggggCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTa                                                                                           <  2:4388865/134‑1 (MQ=1)
                                               ccgttGGCAGAACAACTGGTACAGCAGCGGTGCGTCCATCCCGGTCGTCTCGTACTAAGGACCGCTCCTCGCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCAAGACGTTCGGAACCCAGCTCGCGTAc                                                                                          >  1:2968287/4‑140 (MQ=1)
                                                         aaaaacTTGTACACCAGCGGTTCGTCCATAAAGGAACTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTACTGAACCCAGCTCGCGTACCGCTTTAATg                                                                                >  1:640263/4‑140 (MQ=1)
                                                               tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                          <  2:4661319/137‑1 (MQ=1)
                                                               tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                          <  1:467677/137‑1 (MQ=1)
                                                               tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                          <  2:4640963/137‑1 (MQ=1)
                                                               tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                          <  1:5649845/137‑1 (MQ=1)
                                                               tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGGACCGCTTTAATGGGCGaa                                                                          <  2:4020454/137‑1 (MQ=1)
                                                               tgcgaccccAGCGCGGCGTCCATCCCCGTCCTCACGTACCAAGGACAGCTCCTCTCAAATTTCCTGCCCCCGCGACCGATAGGGACCGAACTGTCACACGACGTTCTGAACCCAGCTCGCGAACCGCTTTAATGGGCGaa                                                                          <  1:3042538/133‑1 (MQ=1)
                                                               cgggacacCCGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                          <  2:3300131/136‑1 (MQ=0)
                                                               acggaccccAGCGGTGCGTCCATCCCGCTCCGCTCGTACTAAGCACACCTCCTCGCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                          <  2:3358332/133‑1 (MQ=1)
                                                                      cctgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                   <  2:3512047/137‑1 (MQ=1)
                                                                      ccggCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                   <  2:3751760/137‑1 (MQ=1)
                                                                      cccgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                   <  2:737406/137‑1 (MQ=1)
                                                                             ggcctccctcccGCTCCTCTCGGACTCAGGACAGCTGCTCTCAAATTTCCTGAGCCCGCGACGGATAGGGACCGAACTGGCTCACGACGTTCTGACCCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTg                                                            <  2:4352478/136‑1 (MQ=1)
                                                                                 tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                          >  2:3903664/7‑122 (MQ=0)
                                                                                 tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                          <  1:3903664/116‑1 (MQ=0)
                                                                                 tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGAc                                                        >  1:1733360/7‑140 (MQ=0)
                                                                                 gccctCCCGGTCCTCTCGTACTCAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGAc                                                        <  1:541993/136‑1 (MQ=0)
                                                                                            ccgctcGTACTAAGCACAGCTCCTCTCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGCGACCGACTACAGcc                                             <  2:4627007/137‑1 (MQ=0)
                                                                                                            catctcctcTCTAATTTCCTGCGCCCGCGACGGATCGGGCCCGAACTGTCTCACGCCGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACGACAGCCCCAGGATGCGATGAGc                             <  1:5029822/137‑1 (MQ=1)
                                                                                                               ctgctctCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCACAGGATGCGATGAGCCGa                          >  1:639076/4‑140 (MQ=1)
                                                                                                               ctgctcgCAAGTTTCCTGCGCCCGCGACGGGTAGGGACCGAACTGTCTCACGACGGTCTGCACCCAGCTCGCGTACCGCTTTAATGGGCGACCAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGa                          >  2:1447534/4‑140 (MQ=1)
                                                                                                                    ctaaaaTTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTATCACGACGTTCTGAACCCATCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGATCCGACATCg                     >  2:3707978/4‑140 (MQ=0)
                                                                                                                             ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa            <  2:1796060/137‑1 (MQ=1)
                                                                                                                             ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa            <  2:1462213/137‑1 (MQ=1)
                                                                                                                             ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa            <  2:1225802/137‑1 (MQ=1)
                                                                                                                                       cgccGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGt  <  1:1758396/137‑1 (MQ=1)
                                                                                                                                           |                                                                                                                                       
AGATGCTTTCAGCACTTATCCCGTCCGCACATAGCTACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGT  >  NZ_CP034943/2978016‑2978290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑