Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,978,186 G→A 14.6% noncoding (2615/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,978,1860GA14.6% 1.9 / 10.4 41noncoding (2615/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (8/27);  new base A (2/4);  total (10/31)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

GCTACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGT  >  NZ_CP034943/2978049‑2978321
                                                                                                                                         |                                                                                                                                       
gcgaCCCAGCGATGCCCTTGGCAGAACAACTGGTACACCCGCGCTGCGTCCATCCCGGTCCGCTCGTACTAAGGACAGCTCCTCTCAAAATTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGaa                                                                                                                                       <  1:168528/137‑1 (MQ=1)
gcgaCCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGaa                                                                                                                                       <  1:4430876/137‑1 (MQ=1)
         cgctGCCCGTGGCAGAACACCTGGTACACCAGCGGTGCGTCCAGCCCGGGCGTCTCGTGCTAAGGAGAGCGGCGCGCAACTTGCCTGCGCGCGCGACGGATCGGGCCCGCACTGTCTCACGACGTGCGGACCCCAGCTcg                                                                                                                              >  2:4186972/4‑140 (MQ=0)
             gcgcTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCGCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTGTGAACCCAGCTCGCGTa                                                                                                                          <  2:3952262/137‑1 (MQ=0)
             gcccggggCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTa                                                                                                                          <  2:4388865/134‑1 (MQ=1)
              ccgttGGCAGAACAACTGGTACAGCAGCGGTGCGTCCATCCCGGTCGTCTCGTACTAAGGACCGCTCCTCGCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCAAGACGTTCGGAACCCAGCTCGCGTAc                                                                                                                         >  1:2968287/4‑140 (MQ=1)
                        aaaaacTTGTACACCAGCGGTTCGTCCATAAAGGAACTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTACTGAACCCAGCTCGCGTACCGCTTTAATg                                                                                                               >  1:640263/4‑140 (MQ=1)
                              tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                         <  2:4661319/137‑1 (MQ=1)
                              tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                         <  2:4640963/137‑1 (MQ=1)
                              tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                         <  1:467677/137‑1 (MQ=1)
                              tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                         <  1:5649845/137‑1 (MQ=1)
                              tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGGACCGCTTTAATGGGCGaa                                                                                                         <  2:4020454/137‑1 (MQ=1)
                              tgcgaccccAGCGCGGCGTCCATCCCCGTCCTCACGTACCAAGGACAGCTCCTCTCAAATTTCCTGCCCCCGCGACCGATAGGGACCGAACTGTCACACGACGTTCTGAACCCAGCTCGCGAACCGCTTTAATGGGCGaa                                                                                                         <  1:3042538/133‑1 (MQ=1)
                              cgggacacCCGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                         <  2:3300131/136‑1 (MQ=0)
                              acggaccccAGCGGTGCGTCCATCCCGCTCCGCTCGTACTAAGCACACCTCCTCGCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                         <  2:3358332/133‑1 (MQ=1)
                                     cctgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                  <  2:3512047/137‑1 (MQ=1)
                                     ccggCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                  <  2:3751760/137‑1 (MQ=1)
                                     cccgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                  <  2:737406/137‑1 (MQ=1)
                                            ggcctccctcccGCTCCTCTCGGACTCAGGACAGCTGCTCTCAAATTTCCTGAGCCCGCGACGGATAGGGACCGAACTGGCTCACGACGTTCTGACCCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTg                                                                                           <  2:4352478/136‑1 (MQ=1)
                                                tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                                                         <  1:3903664/116‑1 (MQ=0)
                                                tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                                                         >  2:3903664/7‑122 (MQ=0)
                                                tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGAc                                                                                       >  1:1733360/7‑140 (MQ=0)
                                                gccctCCCGGTCCTCTCGTACTCAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGAc                                                                                       <  1:541993/136‑1 (MQ=0)
                                                           ccgctcGTACTAAGCACAGCTCCTCTCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGCGACCGACTACAGcc                                                                            <  2:4627007/137‑1 (MQ=0)
                                                                           catctcctcTCTAATTTCCTGCGCCCGCGACGGATCGGGCCCGAACTGTCTCACGCCGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACGACAGCCCCAGGATGCGATGAGc                                                            <  1:5029822/137‑1 (MQ=1)
                                                                              ctgctctCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCACAGGATGCGATGAGCCGa                                                         >  1:639076/4‑140 (MQ=1)
                                                                              ctgctcgCAAGTTTCCTGCGCCCGCGACGGGTAGGGACCGAACTGTCTCACGACGGTCTGCACCCAGCTCGCGTACCGCTTTAATGGGCGACCAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGa                                                         >  2:1447534/4‑140 (MQ=1)
                                                                                   ctaaaaTTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTATCACGACGTTCTGAACCCATCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGATCCGACATCg                                                    >  2:3707978/4‑140 (MQ=0)
                                                                                            ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                           <  2:1796060/137‑1 (MQ=1)
                                                                                            ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                           <  2:1225802/137‑1 (MQ=1)
                                                                                            ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                           <  2:1462213/137‑1 (MQ=1)
                                                                                                      cgccGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGt                                 <  1:1758396/137‑1 (MQ=1)
                                                                                                              agcgACCGCACGGTCTCACGACGTTCTGAACCCAGCGCGCGTACCGCTTTAATGCGCGAACAGCCCAACCCTTGGGACCGACGACACCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTgg                         <  2:4668958/137‑1 (MQ=1)
                                                                                                                      aattGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCAAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTgg                 >  1:1171938/4‑140 (MQ=1)
                                                                                                                       acggTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTACTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                <  2:2578478/137‑1 (MQ=1)
                                                                                                                       acggTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                <  2:4139493/137‑1 (MQ=1)
                                                                                                                       acggTCTCACGACGTTCTGAACCCAGCGCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                <  2:1827593/137‑1 (MQ=1)
                                                                                                                       acggTCTCACGACGGTCTGAACCCAGCTCGCGTACCGCTTTACTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                <  2:4415419/137‑1 (MQ=0)
                                                                                                                       accgTCTCACCCCGTTCAGCACCCAGCCGCCGGAACGCCTTTATGGGCGTACAGCCCAACCCTTGGGAACGCATACACCCCCACGCAGCGCTGACCCGCAATCGATGGTCCAAACCCGCCCGTCGTTGTTGACTCGTggg                <  2:1780327/137‑1 (MQ=0)
                                                                                                                                 gaagTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGc      <  2:866846/137‑1 (MQ=1)
                                                                                                                                     ttatGAACCCAGCTCGCGTAACGCTGTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCACCATGATGCGATGACCATACATCGAGGTGACAAACCTCCCCGACGATGTGGACACTTGGGGGAGATAAGCCTGt  >  2:1953627/4‑140 (MQ=1)
                                                                                                                                         |                                                                                                                                       
GCTACCCAGCGATGCCCTTGGCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGT  >  NZ_CP034943/2978049‑2978321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑