Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,978,204 G→A 16.0% noncoding (2597/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,978,2040GA16.0% 1.2 / 10.6 38noncoding (2597/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (6/25);  new base A (2/4);  total (8/30)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

AACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAG  >  NZ_CP034943/2978073‑2978335
                                                                                                                                   |                                                                                                                                   
aaaaacTTGTACACCAGCGGTTCGTCCATAAAGGAACTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTACTGAACCCAGCTCGCGTACCGCTTTAATg                                                                                                                             >  1:640263/4‑140 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                       <  2:4661319/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                       <  1:467677/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                       <  2:4640963/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                       <  1:5649845/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGGACCGCTTTAATGGGCGaa                                                                                                                       <  2:4020454/137‑1 (MQ=1)
      tgcgaccccAGCGCGGCGTCCATCCCCGTCCTCACGTACCAAGGACAGCTCCTCTCAAATTTCCTGCCCCCGCGACCGATAGGGACCGAACTGTCACACGACGTTCTGAACCCAGCTCGCGAACCGCTTTAATGGGCGaa                                                                                                                       <  1:3042538/133‑1 (MQ=1)
      cgggacacCCGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                       <  2:3300131/136‑1 (MQ=0)
      acggaccccAGCGGTGCGTCCATCCCGCTCCGCTCGTACTAAGCACACCTCCTCGCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                       <  2:3358332/133‑1 (MQ=1)
             cctgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                                <  2:3512047/137‑1 (MQ=1)
             ccggCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                                <  2:3751760/137‑1 (MQ=1)
             cccgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                                <  2:737406/137‑1 (MQ=1)
                    ggcctccctcccGCTCCTCTCGGACTCAGGACAGCTGCTCTCAAATTTCCTGAGCCCGCGACGGATAGGGACCGAACTGGCTCACGACGTTCTGACCCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTg                                                                                                         <  2:4352478/136‑1 (MQ=1)
                        tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                                                                       <  1:3903664/116‑1 (MQ=0)
                        tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                                                                       >  2:3903664/7‑122 (MQ=0)
                        tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGAc                                                                                                     >  1:1733360/7‑140 (MQ=0)
                        gccctCCCGGTCCTCTCGTACTCAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGAc                                                                                                     <  1:541993/136‑1 (MQ=0)
                                   ccgctcGTACTAAGCACAGCTCCTCTCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGCGACCGACTACAGcc                                                                                          <  2:4627007/137‑1 (MQ=0)
                                                   catctcctcTCTAATTTCCTGCGCCCGCGACGGATCGGGCCCGAACTGTCTCACGCCGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACGACAGCCCCAGGATGCGATGAGc                                                                          <  1:5029822/137‑1 (MQ=1)
                                                      ctgctctCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCACAGGATGCGATGAGCCGa                                                                       >  1:639076/4‑140 (MQ=1)
                                                      ctgctcgCAAGTTTCCTGCGCCCGCGACGGGTAGGGACCGAACTGTCTCACGACGGTCTGCACCCAGCTCGCGTACCGCTTTAATGGGCGACCAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGa                                                                       >  2:1447534/4‑140 (MQ=1)
                                                           ctaaaaTTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTATCACGACGTTCTGAACCCATCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGATCCGACATCg                                                                  >  2:3707978/4‑140 (MQ=0)
                                                                    ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                                         <  2:1796060/137‑1 (MQ=1)
                                                                    ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                                         <  2:1462213/137‑1 (MQ=1)
                                                                    ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                                         <  2:1225802/137‑1 (MQ=1)
                                                                              cgccGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGt                                               <  1:1758396/137‑1 (MQ=1)
                                                                                      agcgACCGCACGGTCTCACGACGTTCTGAACCCAGCGCGCGTACCGCTTTAATGCGCGAACAGCCCAACCCTTGGGACCGACGACACCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTgg                                       <  2:4668958/137‑1 (MQ=1)
                                                                                              aattGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCAAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTgg                               >  1:1171938/4‑140 (MQ=1)
                                                                                               acggTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTACTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                              <  2:2578478/137‑1 (MQ=1)
                                                                                               acggTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                              <  2:4139493/137‑1 (MQ=1)
                                                                                               acggTCTCACGACGTTCTGAACCCAGCGCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                              <  2:1827593/137‑1 (MQ=1)
                                                                                               acggTCTCACGACGGTCTGAACCCAGCTCGCGTACCGCTTTACTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                              <  2:4415419/137‑1 (MQ=0)
                                                                                               accgTCTCACCCCGTTCAGCACCCAGCCGCCGGAACGCCTTTATGGGCGTACAGCCCAACCCTTGGGAACGCATACACCCCCACGCAGCGCTGACCCGCAATCGATGGTCCAAACCCGCCCGTCGTTGTTGACTCGTggg                              <  2:1780327/137‑1 (MQ=0)
                                                                                                         gaagTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGc                    <  2:866846/137‑1 (MQ=1)
                                                                                                             ttatGAACCCAGCTCGCGTAACGCTGTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCACCATGATGCGATGACCATACATCGAGGTGACAAACCTCCCCGACGATGTGGACACTTGGGGGAGATAAGCCTGt                >  2:1953627/4‑140 (MQ=1)
                                                                                                                  aagccAGTTCGCGTACCGCTTTAATGGGCGAACAGGCCAACCCTTGGGACCGACTACAGCCCCAGGTTGCGATGAGCCGACATCGAGGTGCCATAACTCCCCGTCGATGTGGACTCTTGGGGGAGATATGCCTGTTATcc           <  2:5390252/137‑1 (MQ=1)
                                                                                                                        gcgcgcgTACCGCTTTAAGGGGCGAACAGCCCAACCCGTGGGACCGACGACCGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCgggg     <  2:4054061/137‑1 (MQ=1)
                                                                                                                           cgagTACCTCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAg  <  2:765582/137‑1 (MQ=1)
                                                                                                                                   |                                                                                                                                   
AACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAG  >  NZ_CP034943/2978073‑2978335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑