Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,978,212 G→T 14.4% noncoding (2589/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,978,2120GT14.4% 2.6 / 10.6 42noncoding (2589/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (5/31);  new base T (2/4);  total (7/35)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

AACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTT  >  NZ_CP034943/2978073‑2978347
                                                                                                                                           |                                                                                                                                       
aaaaacTTGTACACCAGCGGTTCGTCCATAAAGGAACTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTACTGAACCCAGCTCGCGTACCGCTTTAATg                                                                                                                                         >  1:640263/4‑140 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                                   <  2:4661319/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                                   <  2:4640963/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                                   <  1:467677/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                                   <  1:5649845/137‑1 (MQ=1)
      tgctACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGGACCGCTTTAATGGGCGaa                                                                                                                                   <  2:4020454/137‑1 (MQ=1)
      tgcgaccccAGCGCGGCGTCCATCCCCGTCCTCACGTACCAAGGACAGCTCCTCTCAAATTTCCTGCCCCCGCGACCGATAGGGACCGAACTGTCACACGACGTTCTGAACCCAGCTCGCGAACCGCTTTAATGGGCGaa                                                                                                                                   <  1:3042538/133‑1 (MQ=1)
      cgggacacCCGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                                   <  2:3300131/136‑1 (MQ=0)
      acggaccccAGCGGTGCGTCCATCCCGCTCCGCTCGTACTAAGCACACCTCCTCGCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGaa                                                                                                                                   <  2:3358332/133‑1 (MQ=1)
             cctgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                                            <  2:3512047/137‑1 (MQ=1)
             ccggCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                                            <  2:3751760/137‑1 (MQ=1)
             cccgCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCa                                                                                                                            <  2:737406/137‑1 (MQ=1)
                    ggcctccctcccGCTCCTCTCGGACTCAGGACAGCTGCTCTCAAATTTCCTGAGCCCGCGACGGATAGGGACCGAACTGGCTCACGACGTTCTGACCCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTg                                                                                                                     <  2:4352478/136‑1 (MQ=1)
                        tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                                                                                   <  1:3903664/116‑1 (MQ=0)
                        tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGaa                                                                                                                                   >  2:3903664/7‑122 (MQ=0)
                        tccactccGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGAc                                                                                                                 >  1:1733360/7‑140 (MQ=0)
                        gccctCCCGGTCCTCTCGTACTCAGGACAGCTCCTCTCAAATGTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGAc                                                                                                                 <  1:541993/136‑1 (MQ=0)
                                   ccgctcGTACTAAGCACAGCTCCTCTCAAAGTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGCGACCGACTACAGcc                                                                                                      <  2:4627007/137‑1 (MQ=0)
                                                   catctcctcTCTAATTTCCTGCGCCCGCGACGGATCGGGCCCGAACTGTCTCACGCCGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACGACAGCCCCAGGATGCGATGAGc                                                                                      <  1:5029822/137‑1 (MQ=1)
                                                      ctgctctCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCACAGGATGCGATGAGCCGa                                                                                   >  1:639076/4‑140 (MQ=1)
                                                      ctgctcgCAAGTTTCCTGCGCCCGCGACGGGTAGGGACCGAACTGTCTCACGACGGTCTGCACCCAGCTCGCGTACCGCTTTAATGGGCGACCAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGa                                                                                   >  2:1447534/4‑140 (MQ=1)
                                                           ctaaaaTTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTATCACGACGTTCTGAACCCATCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGATCCGACATCg                                                                              >  2:3707978/4‑140 (MQ=0)
                                                                    ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                                                     <  2:1462213/137‑1 (MQ=1)
                                                                    ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                                                     <  2:1225802/137‑1 (MQ=1)
                                                                    ccagcgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCaa                                                                     <  2:1796060/137‑1 (MQ=1)
                                                                              cgccGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGt                                                           <  1:1758396/137‑1 (MQ=1)
                                                                                      agcgACCGCACGGTCTCACGACGTTCTGAACCCAGCGCGCGTACCGCTTTAATGCGCGAACAGCCCAACCCTTGGGACCGACGACACCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTgg                                                   <  2:4668958/137‑1 (MQ=1)
                                                                                              aattGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCAAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTgg                                           >  1:1171938/4‑140 (MQ=1)
                                                                                               acggTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTACTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                                          <  2:2578478/137‑1 (MQ=1)
                                                                                               acggTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                                          <  2:4139493/137‑1 (MQ=1)
                                                                                               acggTCTCACGACGTTCTGAACCCAGCGCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                                          <  2:1827593/137‑1 (MQ=1)
                                                                                               acggTCTCACGACGGTCTGAACCCAGCTCGCGTACCGCTTTACTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTggg                                          <  2:4415419/137‑1 (MQ=0)
                                                                                               accgTCTCACCCCGTTCAGCACCCAGCCGCCGGAACGCCTTTATGGGCGTACAGCCCAACCCTTGGGAACGCATACACCCCCACGCAGCGCTGACCCGCAATCGATGGTCCAAACCCGCCCGTCGTTGTTGACTCGTggg                                          <  2:1780327/137‑1 (MQ=0)
                                                                                                         gaagTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGc                                <  2:866846/137‑1 (MQ=1)
                                                                                                             ttatGAACCCAGCTCGCGTAACGCTGTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCACCATGATGCGATGACCATACATCGAGGTGACAAACCTCCCCGACGATGTGGACACTTGGGGGAGATAAGCCTGt                            >  2:1953627/4‑140 (MQ=1)
                                                                                                                  aagccAGTTCGCGTACCGCTTTAATGGGCGAACAGGCCAACCCTTGGGACCGACTACAGCCCCAGGTTGCGATGAGCCGACATCGAGGTGCCATAACTCCCCGTCGATGTGGACTCTTGGGGGAGATATGCCTGTTATcc                       <  2:5390252/137‑1 (MQ=1)
                                                                                                                        gcgcgcgTACCGCTTTAAGGGGCGAACAGCCCAACCCGTGGGACCGACGACCGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCgggg                 <  2:4054061/137‑1 (MQ=1)
                                                                                                                           cgagTACCTCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAg              <  2:765582/137‑1 (MQ=1)
                                                                                                                                  cggtttAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGTTGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTAt       <  1:2356097/137‑1 (MQ=1)
                                                                                                                                  cgatttAATGGGCGAACAGCCCACCCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTAt       <  2:587955/137‑1 (MQ=1)
                                                                                                                                      ttcaTGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGt   <  2:1510555/137‑1 (MQ=1)
                                                                                                                                       tactGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGtt  <  2:346860/137‑1 (MQ=1)
                                                                                                                                       tactGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGAGGGGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGtt  <  2:4792378/137‑1 (MQ=0)
                                                                                                                                           |                                                                                                                                       
AACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTT  >  NZ_CP034943/2978073‑2978347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑