Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,979,248 T→C 37.7% noncoding (1553/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,979,2480TC37.7% ‑2.7 / 44.2 45noncoding (1553/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (5/23);  new base C (2/15);  total (7/38)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCA  >  NZ_CP034943/2979114‑2979383
                                                                                                                                      |                                                                                                                                       
ctgaGGATTCTCTCCTCGCCGACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGaa                                                                                                                                    <  2:4826188/137‑1 (MQ=1)
 tttggATTCTCTCCTCGCCTACCTGTGTCTGTTTGCGGTACGGGCACCTCTCACCTCGCTATAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGaaa                                                                                                                                   <  1:33583/137‑1 (MQ=0)
         ctatcctcGCCTACCTGTGTCGCTTTGCGGTACCGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTg                                                                                                                           >  1:3195586/4‑140 (MQ=0)
          tcgcctcGCCTACCTGTGTCGGTTTGCTGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGACTCAGGAACTTCGCTACTACATTTCGCTCTCCATCACAGCTCAGACGTATGGGAAACCGATTTGc                                                                                                                          >  1:4805592/4‑140 (MQ=1)
          tcacctcGCCTACCTGTGTCGTTTTTAGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGc                                                                                                                          >  2:2002830/4‑140 (MQ=0)
                  ccgaCCTGTTGCGGTTTGCGGTACGGGCACCTCTCCCCTCGCTAGAGGCTGGTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTGACGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  2:3312221/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCTGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  2:3245943/137‑1 (MQ=0)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTGTGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:5132077/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:4722487/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:1563309/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:2338610/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:2128902/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCGAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  2:3183086/137‑1 (MQ=1)
                  ccgaCCTGGGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATGTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:5615645/137‑1 (MQ=0)
                      ccggtgtCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGc                                                                                                              >  2:2511538/4‑140 (MQ=1)
                                   gctgTACGGGCAGCTCTCACCTCGCTAGAGTCTTTTCTTGTCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACATCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGa                                                                                                 <  1:3089417/137‑1 (MQ=1)
                                        actggCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCgg                                                                                            <  1:4825196/137‑1 (MQ=1)
                                        accggCACCTCTCACCTCGCGAGAGGCTTTTCTGGGCAGTGTGGAATCAGGAACGTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCgg                                                                                            <  1:258076/137‑1 (MQ=0)
                                                   tctccTCGCTAGAGTCTTTTCTTGGCAGTGTGGAATCATGAACTTCGCTACTACTTTTCGCTCGCCATCACAGCTGAGCCTTATGGGAAACGGATTTTCCTATTTCCCAGCCTAACTGCTTTGACTCGGATATCCTATAc                                                                                 <  2:3512114/137‑1 (MQ=1)
                                                   tctccTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTGGCTACTACATTTCGCTCGACATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCGAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  1:2374111/137‑1 (MQ=1)
                                                   tccccTCGCTCGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTGCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATGTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  2:5626859/137‑1 (MQ=1)
                                                   tccccTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  1:1903623/137‑1 (MQ=1)
                                                   tccccTCGCGAGAGGCGTTTCTGGGCAGTGTGGAATCAGGAACTTCGCTACTCCCTGTCGCTCGCCATCACAGCTCCGCCTTCTGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  2:4676071/137‑1 (MQ=1)
                                                        tctcTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCgcgc                                                                            <  2:3483971/137‑1 (MQ=1)
                                                        tctcTAGAGGCTTTTCTTGGAAGTTTTGTATCAGTAACTTCGCTACTACATTTCGCTCGCGATCACAGCTCAGCCTTACGGGAAACGGATTTGCGTATTTCCCAGACTTACTGCTTGGACGCGGATATCCAATACCgcgc                                                                            <  1:5796007/137‑1 (MQ=1)
                                                          gcgagagGGTTTTCTTGGCAGTGTGGAATCAGGAAGTTCGCTACTACATGTGGCTAGCCATCACAGCTCAGCCTTATGGGAAACGGAGTGGCGTAGTTCGCAGCGTCACGGCTTGGACGCGGAGATCCAATCCCgcgcgt                                                                          >  2:315096/4‑138 (MQ=1)
                                                             agatgattttCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTAcc                                                                       <  2:869259/134‑1 (MQ=1)
                                                                                   gactCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAAGACCGCGCTTACCCTATCCTCCTGCGTCCCCCCAt                                                 <  1:1446344/137‑1 (MQ=0)
                                                                                     atgaGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTg                                               <  2:705842/137‑1 (MQ=1)
                                                                                         ggaccgtCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACGGCTTGGACGCGGTTATCCAATACCGCGCTTACCCTCTCCTCCTGCGTCCCCCCCTTGCTCa                                           >  2:4654889/7‑140 (MQ=1)
                                                                                                  ctcctacATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCACAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTgag                                  >  1:1412869/4‑140 (MQ=0)
                                                                                                  ctcctacATATCGCTCCCCATCACAGCTCAGCCTTATGGGAAACTGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGTATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCACCCATTGCTCAAATGTTgag                                  <  2:3277357/137‑1 (MQ=1)
                                                                                                    acgacATGTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTgagga                                <  1:4386435/137‑1 (MQ=0)
                                                                                                      tataTTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg                              <  2:5641474/137‑1 (MQ=1)
                                                                                                      tacctgtcgcgcgcCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg                              <  1:867839/134‑1 (MQ=0)
                                                                                                      gacctttCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg                              <  2:3178857/136‑1 (MQ=1)
                                                                                                         ccctccctcgcCAACACACCACACCCCGACGGGAAACGGATGTGCCTAGTTCCCACCCGAACCGCTTGGACGCGGATATCCAATACCCCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAggtgg                           <  2:3747072/134‑1 (MQ=1)
                                                                                                         atttatctcgcCATCACAGCTCAGCCTTATGGGAATCGGATTTGCCTATTTCACAGACTAACTGCTTGGCCGCGGATCTCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATGGCTCAAATGGTGAGGAggtgg                           <  1:1397201/134‑1 (MQ=1)
                                                                                                         atgtcgctcgcCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGAGATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAggtgg                           <  1:645748/137‑1 (MQ=1)
                                                                                                         atgtcgctcgcCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAggtgg                           <  2:3065404/137‑1 (MQ=1)
                                                                                                              gatctccATGACATCTCAGCCTTATGGGAAACGGATTGGACTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCACTACCGCGCTGACCCGATCCTCCTGCGTCCCCCCCTTGCTCAAATGGTGAGGAGGTGGTACAg                      <  1:3258404/135‑1 (MQ=1)
                                                                                                                gcgacATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGa                    <  2:4118136/136‑1 (MQ=1)
                                                                                                                           tctcatacTTACGGGAAACGTATTTGCCTATTTCCCAGCGTATCTGCTTGTACGCGGATATCCTATTCTGCGATTACCCTATCCTACTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCTACCtg         <  2:5660422/133‑1 (MQ=1)
                                                                                                                                  ctgaTGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCa  <  2:5490498/137‑1 (MQ=1)
                                                                                                                                  ctgaTGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAAGACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCa  <  1:3327315/137‑1 (MQ=1)
                                                                                                                                      |                                                                                                                                       
CTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCA  >  NZ_CP034943/2979114‑2979383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑