Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,600 T→A 5.5% intergenic (+278/‑26) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,6000TA 5.5% 1511.0 / 9.3 580intergenic (+278/‑26)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (168/357);  new base A (33/9);  total (204/376)
Fisher's exact test for biased strand distribution p-value = 3.61e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 580 total aligned reads displayed.

TCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGC  >  NZ_CP034943/112462‑112738
                                                                                                                                           |                                                                                                                                          
    aGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTGTTTGGTGGAGAGGAAAAGAAAt                                                                                                                                       >  1:3912127/1‑140 (MQ=255)
       cggcCGCCGCCGTTCCAGGCTGAAGTTGGGCGAGCAGCCGTCTGCTTTTCCAACAGAGTGGACCCGCGCGGATGAAGCGTCTCTGTCATGTTTTCATGAAGAGGCGCTTTTTTTATGGGGGCAAGTGGTAATCAAATcga                                                                                                                                    >  2:3327358/1‑140 (MQ=255)
       cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATaga                                                                                                                                    >  1:4252178/1‑140 (MQ=255)
        ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                                   <  1:4526830/140‑1 (MQ=255)
        ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                                   <  1:3670905/140‑1 (MQ=255)
         gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATTCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATGAAATagaca                                                                                                                                  >  2:4047712/1‑138 (MQ=255)
         gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAgaga                                                                                                                                  >  1:77486/1‑140 (MQ=255)
         gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCACATAgaga                                                                                                                                  >  2:3725536/1‑140 (MQ=255)
         gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTGTTTTTTTTTGGGGAAGAGGAAATACAATAgaga                                                                                                                                  >  1:3350256/1‑140 (MQ=255)
          ccggcgccgTTACAGGCTGAAGGTGGGAGAGCAGAAGTCTGCTTTTCATTCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAAACAAATAGAGaa                                                                                                                                 <  2:1136983/140‑1 (MQ=255)
          ccgccgcagTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGaa                                                                                                                                 <  2:2177148/140‑1 (MQ=255)
                ccgTAACCGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCACACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGATTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggg                                                                                                                           <  1:5278049/140‑1 (MQ=255)
                  gTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggggg                                                                                                                         <  1:1768742/140‑1 (MQ=255)
                      cAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                                                                                     <  1:1246066/140‑1 (MQ=255)
                        ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                                                                                                   <  2:5491461/140‑1 (MQ=255)
                           tGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAGCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGAAGCAtgtg                                                                                                                >  2:2659850/1‑140 (MQ=255)
                             aaGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCTTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGtttt                                                                                                              >  2:2741460/1‑140 (MQ=255)
                                                cttcTTTTCAAACAGATTGGAACCGCGCTGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTTTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  1:3504563/137‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  1:2824374/140‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  1:4619240/140‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  1:1519462/140‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  1:4934921/140‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  1:1666306/140‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  2:3023023/140‑1 (MQ=255)
                                                cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                           <  2:5678413/140‑1 (MQ=255)
                                                 tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGTAGAGGAAATCAAATATAGAAGTGGGGGGAACCCGGTGTTTTTTTGTATTGTCAaataaa                                                                                          >  1:1888194/1‑139 (MQ=255)
                                                 tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTTGGGGGACGCCGGTGTTTTTTTTAAATCTCAaagaag                                                                                          >  1:2722984/1‑140 (MQ=255)
                                                  gattttCACACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGATGAAATCAAATAGAGAAGTGGGGGCAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  2:5078849/138‑1 (MQ=255)
                                                  gattttAAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGAGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  1:5440837/138‑1 (MQ=255)
                                                  gCTTTTCAAACAGAGTGGAACCGGGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  2:4839007/140‑1 (MQ=255)
                                                  gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  1:5098966/140‑1 (MQ=255)
                                                  gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  2:4748050/140‑1 (MQ=255)
                                                  gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  2:3604508/140‑1 (MQ=255)
                                                  gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  1:4414831/140‑1 (MQ=255)
                                                  gCTTTTCAAACAGAGTGGAACCGCGCGGTGTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  2:2309211/140‑1 (MQ=255)
                                                   cTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGAc                                                                                        <  1:4695676/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGACATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  1:1077712/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  2:775797/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  2:1820456/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  1:5590698/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  1:564014/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  2:1721340/140‑1 (MQ=255)
                                                          aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  1:709436/140‑1 (MQ=255)
                                                           aCAGTGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTATTGGGTGAAGAGGAAAACACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                 <  1:1015190/140‑1 (MQ=255)
                                                                gTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGGATTGGAAATCAAATAAAGAAGTTGGGGGGAGCCAGGTTTTTTTATTATTCACAAATAACACAATtataaaaggt                                                                           >  1:3775340/1‑134 (MQ=255)
                                                                gTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTGGGTGTATAGGAAAAAAAAAATAGTAGTGGGGGGAAGCACTTGTTTTTTATTATTCTCAAAAAAAAAAATTATAAAgtgt                                                                           >  1:2839895/1‑140 (MQ=255)
                                                                 tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                                          <  1:2593778/140‑1 (MQ=255)
                                                                  cgAACCGCGCGGTTTAAGCGGCTCTGTCATGTTTTCATTCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTc                                                                         <  2:3111575/139‑1 (MQ=255)
                                                                       cgcgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCa                                                                    <  2:4352658/140‑1 (MQ=255)
                                                                         cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTGGGTGAAGAGGAAAAAAAATATAGAAGTGGGGGGGAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATAAAGTGTTGGATCAAg                                                                  >  1:3048327/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGACGAGGAAAACAAATAGAGAACTGGGGGGAAGCATGTGTTTTTTAGAATGCACAAAGAACAAAATGATACAGTGGTAGAGAAAGAAACAGCAGCaagaa                                                   >  2:1486136/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATGAAATAGGGAAGTGGGGGGACGCCTGTGTTTTTTAGAATGGTCCAAGAAGACATTGATCCAGTGTTCGATCATGATCCAGCAGCgagaa                                                   >  2:5174439/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATATAGAAGTGGGGGGAAGCAGGTGTTTTTTAGAATGCTCAAAGAAGACATTGTTACAGTTTTAGATCAATATCCAGCAGCaagaa                                                   >  2:1390620/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTTGAATGCTCAAAGAATACATTGATACAGTTTTCGATCAAGATACAGCAGCaagaa                                                   >  2:5402458/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTCGATAAAGATCCAGCAGCaagaa                                                   >  2:1015972/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                   >  2:5748101/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGATCAAACAAGACATTGATACAGTGTTAGATAAAGATACAGCCGcaacaa                                                   >  2:3500836/1‑140 (MQ=255)
                                                                                        tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGGAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCCTGTGTTTTTTATAATTCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                   >  2:347593/1‑140 (MQ=255)
                                                                                         ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                                  <  1:1401771/140‑1 (MQ=255)
                                                                                         ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                                  <  1:4479193/140‑1 (MQ=255)
                                                                                         ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                                  <  1:395942/140‑1 (MQ=255)
                                                                                                 ttttCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATCAAATATAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACATTTATAAAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTAttttt                                          >  2:459464/1‑139 (MQ=255)
                                                                                                 ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                         <  1:341610/93‑1 (MQ=255)
                                                                                                 ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAGTGTTCGATAAAGATCCAGCAGCAAGAAGGTAttttt                                          >  2:3975031/1‑139 (MQ=255)
                                                                                                 ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTg                                          >  2:2536506/1‑140 (MQ=255)
                                                                                                  tttCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATCAAATATAGGAGTGGGGGGCAGCCTGGGTTTTTTTTAATGCGCAAAGAAGACATTGTTACAGTGTTAGATAAAGATACAGCAGCAAGAAGTTAATTTGa                                         >  1:4326670/1‑140 (MQ=255)
                                                                                                   ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                                        <  1:3578919/140‑1 (MQ=255)
                                                                                                      aTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGCAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAGTGTTCGATCAAGATCCAGCAGCCAGAAGGTATTTTGAAGTg                                     >  2:4959547/1‑140 (MQ=255)
                                                                                                         cAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAACGGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAtt                                  >  2:1219709/1‑140 (MQ=255)
                                                                                                           gagGCGCTTTTTTTATTTGGTGTAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCCTGGGTTTTTTTTAATGCTCAAATAAAACAATGATAAAGTGTTAGATGAAAATCCACCAGCAAGAAGTTATTTTTAAGTGAtttt                                >  1:4068440/1‑140 (MQ=255)
                                                                                                           gagGCGCTTTTTTTATTGGGTGAATAGGAAATAAAAAAGAGGAGTGGGGGGGAGCATGTGTTTTTTAGTATTCTAAAAAAAGACCTTGATACAGTGTTAGATCAAGATCCACCAGCAAGAAATTATTTTGTAGTGAtttt                                >  2:83963/1‑140 (MQ=255)
                                                                                                                     tttttATTGGGTGGAGAGGGAATCAAATAGAGAAGTGGGGGGCATCAAGTGTTTTTTATAATGCTCAAATAACACAATGATACAATGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTTAGTTTAAGTTATTc                       >  2:622658/1‑140 (MQ=255)
                                                                                                                     tttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc                       <  2:2829031/140‑1 (MQ=255)
                                                                                                                     ttattATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc                       <  1:5043758/137‑1 (MQ=255)
                                                                                                                        ttAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCgg                    <  2:1624583/140‑1 (MQ=255)
                                                                                                                          cttGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTGAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGtt                  <  1:5777770/139‑1 (MQ=255)
                                                                                                                            tGGGTGAAGAGGAAATCACATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                <  1:2018657/140‑1 (MQ=255)
                                                                                                                            tGGGTGAAGAGGAAATCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                <  1:640691/140‑1 (MQ=255)
                                                                                                                            tGGGTGAAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTGTAACTTATTCCGGTTTa                <  2:2107839/140‑1 (MQ=255)
                                                                                                                            tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                <  2:3858967/140‑1 (MQ=255)
                                                                                                                            tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                <  1:856294/140‑1 (MQ=255)
                                                                                                                             gggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAc               >  1:4752487/1‑140 (MQ=255)
                                                                                                                              ggTGCAGAGGACATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:3315370/140‑1 (MQ=255)
                                                                                                                              ggTGAAGCGCACATCAAAGAGCGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:326392/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGACATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTAGAAGGCTCACAGAAGACATTGATACAGGGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:2198324/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAACTCAACTAGAGCAGTGGGGGGAAGCATGTGTTTTTGAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:4159610/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGCAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:3857877/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:2141159/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:1855086/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:1937506/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:3407329/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:2010064/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:447252/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:4099019/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:2448585/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  1:2977689/140‑1 (MQ=255)
                                                                                                                              ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGATTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa              <  2:4176348/140‑1 (MQ=255)
                                                                                                                                    gagGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCtat        <  2:876971/140‑1 (MQ=255)
                                                                                                                                      ggAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTatat      <  2:5618142/140‑1 (MQ=255)
                                                                                                                                        aaaTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATgg    >  1:3105487/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGC  >  NZ_CP034943/112462‑112738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: